The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.
Human MIEF1 recruits Drp1 to mitochondrial outer membranes and promotes mitochondrial fusion rather than fission
Open accessAbstract
Mitochondrial morphology is controlled by two opposing processes: fusion and fission. Drp1 (dynamin-related protein 1) and hFis1 are two key players of mitochondrial fission, but how Drp1 is recruited to mitochondria and how Drp1-mediated mitochondrial fission is regulated in mammals is poorly understood. Here, we identify the vertebrate-specific protein MIEF1 (mitochondrial elongation factor 1; independently identified as MiD51), which is anchored to the outer mitochondrial membrane. Elevated MIEF1 levels induce extensive mitochondrial fusion, whereas depletion of MIEF1 causes mitochondrial fragmentation. MIEF1 interacts with and recruits Drp1 to mitochondria in a manner independent of hFis1, Mff (mitochondrial fission factor) and Mfn2 (mitofusin 2), but inhibits Drp1 activity, thus executing a negative effect on mitochondrial fission. MIEF1 also interacts with hFis1 and elevated hFis1 levels partially reverse the MIEF1-induced fusion phenotype. In addition to inhibiting Drp1, MIEF1 also actively promotes fusion, but in a manner distinct from mitofusins. In conclusion, our findings uncover a novel mechanism which controls the mitochondrial fusion-fission machinery in vertebrates. As MIEF1 is vertebrate-specific, these data also reveal important differences between yeast and vertebrates in the regulation of mitochondrial dynamics.
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“MIEF1/MiD51 and the variant MIEF2/MiD49 recruit DRP1 to mitochondria independent of MFF, but in contrast to MFF, inhibit DRP1 GTPase activity leading to mitochondrial elongation when MiD49/51 is overexpressed [92, 95, 96].”
From The connection between the dynamic remodeling of the mitochondrial network and the regulation of muscle mass (2021) · cites this paper as [95]
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Cites
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Mitochondrial dynamics: overview of molecular mechanisms
Tilokani, L., Nagashima, S., Paupe, V., & Prudent, J. (2018) · Essays in Biochemistry · doi:10.1042/ebc20170104 · Cited by 1,397
Fis1, Mff, MiD49, and MiD51 mediate Drp1 recruitment in mitochondrial fission
Losón, O. C., Song, Z., Chen, H., & Chan, D. C. (2013) · Molecular Biology of the Cell · doi:10.1091/mbc.e12-10-0721 · Cited by 1,235
Fusion and fission: interlinked processes critical for mitochondrial health
Chan, D. C. (2012) · Annual Review of Genetics · doi:10.1146/annurev-genet-110410-132529 · Cited by 1,196
Mitochondrial Dynamics — Mitochondrial Fission and Fusion in Human Diseases
Archer, S. L. (2013) · New England Journal of Medicine · doi:10.1056/nejmra1215233 · Cited by 1,120
Mechanisms of Mitochondrial Fission and FusionPreprint
van der Bliek, A. M., Shen, Q., & Kawajiri, S. (2013) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a011072 · Cited by 903
Metadata
- Authors
- Zhao, J., Liu, T., Jin, S., Wang, X., Qu, M., Uhlén, P., Tomilin, N., Shupliakov, O., Lendahl, U., & Nistér, M.
- Affiliations
- Karolinska University Hospital (SE)
- Karolinska Institutet (SE)
- Corresponding author
- Zhao, Jian
- Lendahl, Urban
- Nistér, Monica
- Venue
- The EMBO Journal
- Published
- 2011-06-24
- Volume
- 30
- Issue
- 14
- Pages
- 2762-2778
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- PMC
- PMC3160255
- OpenAlex
- W1960363048
- Cited by
- 370
- References
- 54
- Citations by year
- 2026: 8 · 2025: 13 · 2024: 14 · 2023: 22 · 2022: 17 · 2021: 30 · 2020: 25 · 2019: 19 · 2018: 27 · 2017: 27 · 2016: 27 · 2015: 33 · 2014: 38 · 2013: 37 · 2012: 26
- FWCI
- 14.04
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- The EMBO Journal (publishedVersion, cc-by-nc-sa)
- Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- University of Rochester
- Cancerfonden
- Karolinska Institutet
- Knut och Alice Wallenbergs Stiftelse
- Vetenskapsrådet
- Deutsches Zentrum für Neurodegenerative Erkrankungen
- Barncancerfonden
- Cancerföreningen i Stockholm
- National Institutes of Health
- National Institute of Neurological Disorders and Stroke
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Keywords
- MiD51, Drp1, mitochondrial fusion, mitochondrial fission, mitochondrial dynamics, mitochondrial outer membrane, mitochondrial fragmentation, mitofusins
- MeSH terms
- Cross-Linking Reagents, Cytoplasm, Fluorescent Antibody Technique, Glioma, HeLa Cells, Humans, Immunoenzyme Techniques, Membrane Fusion, Membrane Proteins, Microtubule-Associated Proteins, Mitochondria, Neuroblastoma, Peptide Elongation Factors, Protein Binding, RNA, Messenger, Subcellular Fractions, Tumor Cells, Cultured, Blotting, Western, Apoptosis, Reverse Transcriptase Polymerase Chain Reaction, GTP Phosphohydrolases, Mitochondrial Proteins, Dynamins, RNA, Small Interfering, Immunoprecipitation, Mitochondrial Membranes
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