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Cited by 269Antioxidant Activity and Oxidative StressNF-κB Signaling PathwaysNatural Compounds in Disease Treatment

β-Carotene inhibits inflammatory gene expression in lipopolysaccharide-stimulated macrophages by suppressing redox-based NF-κB activation

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Bai, Se-Kyung, Lee, Seon-Jin, Na, Hee-Jun, Ha, Kwon-Soo, Han, Jeong-A, Lee, Hansoo, Kwon, Young-Guen, Chung, Cha-Kwon +1 more·2005·Experimental & Molecular Medicine
DOI 10.1038/emm.2005.42
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Abstract

beta-Carotene has shown antioxidant and anti-inflammatory activities; however, its molecular mechanism has not been clearly defined. We examined in vitro and in vivo regulatory function of beta-carotene on the production of nitric oxide (NO) and PGE(2) as well as expression of inducible NO synthase (iNOS), cyclooxygenase-2, TNF-alpha, and IL-1beta. beta-Carotene inhibited the expression and production of these inflammatory mediators in both LPS-stimulated RAW264.7 cells and primary macrophages in a dose-dependent fashion as well as in LPS-administrated mice. Furthermore, this compound suppressed NF-kappaB activation and iNOS promoter activity in RAW264.7 cells stimulated with LPS. beta-Carotene blocked nuclear translocation of NF-kappaB p65 subunit, which correlated with its inhibitory effect on IkappaBalpha phosphorylation and degradation. This compound directly blocked the intracellular accumulation of reactive oxygen species in RAW264.7 cells stimulated with LPS as both the NADPH oxidase inhibitor diphenylene iodonium and antioxidant pyrrolidine dithiocarbamate did. The inhibition of NADPH oxidase also inhibited NO production, iNOS expression, and iNOS promoter activity. These results suggest that beta-carotene possesses anti-inflammatory activity by functioning as a potential inhibitor for redox-based NF-kappaB activation, probably due to its antioxidant activity.

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  • β-carotene directly blocked intracellular accumulation of ROS in murine macrophage cell line RAW264.7.

    “In addition, Bai et al. showed that β-carotene directly blocked the intracellular accumulation of ROS in murine macrophage cell line RAW264.7 [35]”

    From Beneficial Effects of Spirulina Consumption on Brain Health (2022) · cites this paper as [35]

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Authors
Bai, S.-K., Lee, S.-J., Na, H.-J., Ha, K.-S., Han, J.-A., Lee, H., Kwon, Y.-G., Chung, C.-K., & Kim, Y.-M.
Affiliations
  • Kangwon National University (KR)
  • Hallym University (KR)
ORCID
Venue
Experimental & Molecular Medicine
Publisher
Springer Science and Business Media LLC
Published
2005-08-01
Volume
37
Issue
4
Pages
323-334
ISSN
  • 1226-3613 (ISSN-L)
  • 2092-6413
DOI
10.1038/emm.2005.42
OpenAlex
W2030470859
Cited by
269
References
20
Citations by year
2026: 11 · 2025: 18 · 2024: 13 · 2023: 14 · 2022: 22 · 2021: 25 · 2020: 19 · 2019: 14 · 2018: 7 · 2017: 18 · 2016: 14 · 2015: 9 · 2014: 11 · 2013: 8 · 2012: 11
FWCI
3.46
Type
journal article
Language
en
Open access
Licence
Locations
  • Experimental & Molecular Medicine (publishedVersion, cc-by-nc)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › NF-κB Signaling Pathways
  • Health Sciences › Medicine › Complementary and alternative medicine › Natural Compounds in Disease Treatment
Keywords
beta-carotene, anti-inflammatory activity, NF-κB activation, redox signaling, macrophages, lipopolysaccharide, nitric oxide, inducible nitric oxide synthase, cyclooxygenase-2, TNF-α, IL-1β, reactive oxygen species, NADPH oxidase, IκBα phosphorylation, antioxidant activity
MeSH terms
Animals, Anti-Inflammatory Agents, Antioxidants, Female, Lipopolysaccharides, Macrophages, Mice, Inbred BALB C, Nitric Oxide, Oxidation-Reduction, Dinoprostone, Gene Expression, NF-kappa B, Inflammation Mediators, beta Carotene, Mice

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