Cited by 141Nutritional Studies and DietPhytochemicals and Antioxidant ActivitiesExercise and Physiological Responses

Fruit and vegetable consumption and proinflammatory gene expression from peripheral blood mononuclear cells in young adults: a translational study

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Hermsdorff, Helen Hermana M, Zulet, María Ángeles, Puchau, Blanca, Martínez, José Alfredo·2010·Nutrition & Metabolism

Abstract

BACKGROUND: Fruits and vegetables are important sources of fiber and nutrients with a recognized antioxidant capacity, which could have beneficial effects on the proinflammatory status as well as some metabolic syndrome and cardiovascular disease features. The current study assessed the potential relationships of fruit and vegetable consumption with the plasma concentrations and mRNA expression values of some proinflammatory markers in young adults. METHODS: One-hundred and twenty healthy subjects (50 men/70 women; 20.8 +/- 2.6 y; 22.3 +/- 2.8 kg/m2) were enrolled. Experimental determinations included anthropometry, blood pressure and lifestyle features as well as blood biochemical and inflammatory measurements. The mRNA was isolated from peripheral blood mononuclear cells (PBMC) and the gene expression concerning selected inflammatory markers was assessed by quantitative real-time PCR. Nutritional intakes were estimated by a validated semi-quantitative food-frequency questionnaire. RESULTS: The highest tertile of energy-adjusted fruit and vegetable consumption (>660 g/d) was associated with lower plasma concentrations of C-reactive protein (CRP) and homocysteine and with lower ICAM1, IL1R1, IL6, TNFalpha and NFkappaB1 gene expression in PBMC (P for trend < 0.05), independently of gender, age, energy intake, physical activity, smoking, body mass index, systolic blood pressure and circulating non-esterified fatty acids. In addition, plasma CRP, homocysteine and TNFalpha concentrations and ICAM1, TNFalpha and NFkappaB1 gene expression in PBMC showed a descending trend as increased fiber intake (>19.5 g/d) from fruits and vegetables (P for trend < 0.05). Furthermore, the participants within the higher tertile (>11.8 mmol/d) of dietary total antioxidant capacity showed lower plasma CRP and mRNA values of ICAM1, IL1R1, IL6, TNFalpha and NFkappaB1 genes (P for trend < 0.05). The inverse association between fruit and vegetable consumption and study proinflammatory markers followed the same trend and remained statistically significant, after the inclusion of other foods/nutrients in the linear regression models. CONCLUSION: A higher fruit and vegetable consumption was independently associated not only with reduced CRP and homocysteine concentrations but also with a lower mRNA expression in PBMC of some relevant proinflammatory markers in healthy young adults.

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  • Grimble et al. showed that the ability of fish oil to lower LPS-stimulated TNF-a production by blood mononuclear cells was determined in part by polymorphisms within TNF-a and TNF-b genes.

    “This was first identified by Grimble et al. (649) who showed that the ability of fish oil to lower the LPS-stimulated production of TNF-a by blood mononuclear cells was determined in part by polymorphisms within the TNF-a and TNF-b genes.”

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Authors
Hermsdorff, H. H. M., Zulet, M. Á., Puchau, B., & Martínez, J. A.
Affiliations
Universidad de Navarra (ES)
Corresponding author
Martínez, José Alfredo
Venue
Nutrition & Metabolism
Published
2010-05-13
Volume
7
Issue
1
Pages
42-42
ISSN
1743-7075 (ISSN-L)
OpenAlex
W2161028038
Cited by
141
References
58
Citations by year
2026: 2 · 2025: 7 · 2024: 8 · 2023: 7 · 2022: 4 · 2021: 14 · 2020: 9 · 2019: 11 · 2018: 13 · 2017: 9 · 2016: 6 · 2015: 8 · 2014: 8 · 2013: 13 · 2012: 6
FWCI
11.13
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Nutrition & Metabolism (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Deposito Adademico Digital Universidad De Navarra (University of Navarra) (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
Funders
  • Universidad de Navarra (LE/97)
  • Gobierno de Navarra
Topics
  • Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Nutritional Studies and Diet
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
Keywords
fruit and vegetable intake, dietary fiber, pro-inflammatory gene expression, peripheral blood mononuclear cells, C-reactive protein, homocysteine, TNF-α, NFKB1, ICAM-1, IL-1 receptor, interleukin-6, total antioxidant capacity, young adults, inflammation

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