The DOI matches its Crossref record; 92 of the 92 references it cites were found in a registry or an index.
Community-wide transcriptome of the oral microbiome in subjects with and without periodontitis
Open accessAbstract
Despite increasing knowledge on phylogenetic composition of the human microbiome, our understanding of the in situ activities of the organisms in the community and their interactions with each other and with the environment remains limited. Characterizing gene expression profiles of the human microbiome is essential for linking the role of different members of the bacterial communities in health and disease. The oral microbiome is one of the most complex microbial communities in the human body and under certain circumstances, not completely understood, the healthy microbial community undergoes a transformation toward a pathogenic state that gives rise to periodontitis, a polymicrobial inflammatory disease. We report here the in situ genome-wide transcriptome of the subgingival microbiome in six periodontally healthy individuals and seven individuals with periodontitis. The overall picture of metabolic activities showed that iron acquisition, lipopolysaccharide synthesis and flagellar synthesis were major activities defining disease. Unexpectedly, the vast majority of virulence factors upregulated in subjects with periodontitis came from organisms that are not considered major periodontal pathogens. One of the organisms whose gene expression profile was characterized was the uncultured candidate division TM7, showing an upregulation of putative virulence factors in the diseased community. These data enhance understanding of the core activities that are characteristic of periodontal disease as well as the role that individual organisms in the subgingival community play in periodontitis.
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“Application of transcriptomic approaches has proved extremely significant for delineating both microbial and host gene expression in the context of oral health and pathology.14, 68, 94”
From The oral microbiome: Role of key organisms and complex networks in oral health and disease (2021) · cites this paper as [68]
In the citing paper
“Culture‐independent methods have improved our understanding of microbial diversity in the oral cavity.”
From The oral microbiome: Role of key organisms and complex networks in oral health and disease (2021) · cites this paper as [68]
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Cites
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REVIGO summarizes and visualizes long lists of gene ontology terms
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Legendre, P., & Gallagher, E. D. (2001) · Oecologia · doi:10.1007/s004420100716 · Cited by 5,556
Microbial complexes in subgingival plaque
Socransky, S. S., Haffajee, A. D., Cugini, M. A., Smith, C., & Kent, R. L. (1998) · Journal of Clinical Periodontology · doi:10.1111/j.1600-051x.1998.tb02419.x · Cited by 5,050
High-throughput functional annotation and data mining with the Blast2GO suite
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Self-citationBacterial Diversity in Human Subgingival Plaque
Paster, B. J., Boches, S. K., Galvin, J. L., Ericson, R. E., Lau, C. N., Levanos, V. A., Sahasrabudhe, A., & Dewhirst, F. E. (2001) · Journal of Bacteriology · doi:10.1128/jb.183.12.3770-3783.2001 · Cited by 2,008
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Prevalence of Periodontitis in Adults in the United States: 2009 and 2010
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Cited by
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Microbiota in health and diseases
Hou, K., Wu, Z.-X., Chen, X.-Y., Wang, J.-Q., Zhang, D., Xiao, C., Zhu, D., Koya, J. B., Wei, L., Li, J., et al. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-00974-4 · Cited by 3,171
“In periodontal disease, inflammation has been found to be an important driver for the growth of pathogenic microorganisms since inflammation can cause tissue destruction, which provides nutrient to microbiomes.(108) However, inflammation could subsequently trigger bactericidal activity of the immune system.”
BackgroundThe Oral Microbiota: Dynamic Communities and Host Interactions
Lamont, R. J., Koo, H., & Hajishengallis, G. (2018) · Nature Reviews Microbiology · doi:10.1038/s41579-018-0089-x · Cited by 2,217
Species-level functional profiling of metagenomes and metatranscriptomes
Franzosa, E. A., McIver, L. J., Rahnavard, G., Thompson, L. R., Schirmer, M., Weingart, G., Lipson, K. S., Knight, R., Caporaso, J. G., Segata, N., et al. (2018) · Nature Methods · doi:10.1038/s41592-018-0176-y · Cited by 1,693
The oral microbiome: Role of key organisms and complex networks in oral health and disease
Sedghi, L., DiMassa, V., Harrington, A., Lynch, S. V., & Kapila, Y. L. (2021) · Periodontology 2000 · doi:10.1111/prd.12393 · Cited by 755
The role of the microbiota in periodontal disease
Curtis, M. A., Diaz, P. I., & Van Dyke, T. E. (2020) · Periodontology 2000 · doi:10.1111/prd.12296 · Cited by 698
Metadata
- Authors
- Duran-Pinedo, A. E., Chen, T., Teles, R., Starr, J. R., Wang, X., Krishnan, K., & Frias-Lopez, J.
- Affiliations
- The Forsyth Institute (US)
- Harvard University (US)
- Department of Microbiology, Forsyth Institute , Cambridge, MA, USA
- Venue
- The ISME Journal
- Publisher
- Oxford University Press (OUP)
- Published
- 2014-03-06
- Volume
- 8
- Issue
- 8
- Pages
- 1659-1672
- ISSN
- 1751-7362 (ISSN-L)
- 1751-7370
- PMC
- PMC4817619
- OpenAlex
- W2080580603
- Cited by
- 327
- References
- 92
- Citations by year
- 2026: 15 · 2025: 19 · 2024: 17 · 2023: 21 · 2022: 36 · 2021: 42 · 2020: 38 · 2019: 26 · 2018: 25 · 2017: 28 · 2016: 21 · 2015: 26 · 2014: 12
- FWCI
- 38.27
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- The ISME Journal (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- National Institute of Dental and Craniofacial Research (DE021127, U01DE021127, DE021553, R01DE021553)
- Topics
- Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
- Keywords
- periodontitis, oral microbiota, subgingival microbiota, microbial gene expression, virulence factors, iron acquisition, lipopolysaccharide biosynthesis, flagellar synthesis, polymicrobial disease, microbial interactions
- MeSH terms
- Adult, Bacteria, Humans, Periodontitis, Phylogeny, Virulence Factors, Metagenome, Transcriptome, Microbiota
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