The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.
Genome‐scale engineering for systems and synthetic biology
Open accessAbstract
Genome‐modification technologies enable the rational engineering and perturbation of biological systems. Historically, these methods have been limited to gene insertions or mutations at random or at a few pre‐defined locations across the genome. The handful of methods capable of targeted gene editing suffered from low efficiencies, significant labor costs, or both. Recent advances have dramatically expanded our ability to engineer cells in a directed and combinatorial manner. Here, we review current technologies and methodologies for genome‐scale engineering, discuss the prospects for extending efficient genome modification to new hosts, and explore the implications of continued advances toward the development of flexibly programmable chasses, novel biochemistries, and safer organismal and ecological engineering.
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Citations
Cites
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Cited by
Multigene editing in the Escherichia coli genome via the CRISPR-Cas9 system
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Pleiotropy in complex traits: challenges and strategies
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Systems Biology of Microbial Exopolysaccharides Production
Ates, O. (2015) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2015.00200 · Cited by 283
Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria
Farasat, I., Kushwaha, M., Collens, J., Easterbrook, M., Guido, M., & Salis, H. M. (2014) · Molecular Systems Biology · doi:10.15252/msb.20134955 · Cited by 235
Zhen, S., Hua, L., Liu, Y.-H., Gao, L.-C., Fu, J., Wan, D.-Y., Dong, L.-H., Song, H.-F., & Gao, X. (2015) · Gene Therapy · doi:10.1038/gt.2015.2 · Cited by 215
Metadata
- Authors
- Esvelt, K. M., & Wang, H. H.
- Venue
- Molecular Systems Biology
- Published
- 2013-01
- Volume
- 9
- Issue
- 1
- Pages
- 641-641
- ISSN
- 1744-4292 (ISSN-L)
- OpenAlex
- W2167299815
- Cited by
- 320
- References
- 19
- FWCI
- 18.83
- Type
- journal article
- Language
- en
- Open access
- gold
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