Cited by 1,945Microbial Metabolic Engineering and BioproductionBiofuel production and bioconversionBiochemical and biochemical processes

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Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels

Atsumi, Shota, Hanai, Taizo, Liao, James C.·2008·Nature

DOI 10.1038/nature06450

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Abstract

Global energy and environmental problems have stimulated increased efforts towards synthesizing biofuels from renewable resources. Compared to the traditional biofuel, ethanol, higher alcohols offer advantages as gasoline substitutes because of their higher energy density and lower hygroscopicity. In addition, branched-chain alcohols have higher octane numbers compared with their straight-chain counterparts. However, these alcohols cannot be synthesized economically using native organisms. Here we present a metabolic engineering approach using Escherichia coli to produce higher alcohols including isobutanol, 1-butanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from glucose, a renewable carbon source. This strategy uses the host's highly active amino acid biosynthetic pathway and diverts its 2-keto acid intermediates for alcohol synthesis. In particular, we have achieved high-yield, high-specificity production of isobutanol from glucose. The strategy enables the exploration of biofuels beyond those naturally accumulated to high quantities in microbial fermentation.

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Authors: Atsumi, S., Hanai, T., & Liao, J. C.
Affiliations: University of California, Los Angeles (US)
Corresponding author: Liao, James C.
ORCID: Atsumi, Shota
Hanai, Taizo
Liao, James C.
Venue: Nature
Publisher: Springer Science and Business Media LLC
Published: 2008-01
Volume: 451
Issue: 7174
Pages: 86-89
ISSN: 0028-0836 (ISSN-L)
1476-4687
DOI: 10.1038/nature06450
OpenAlex: W1978119727
Cited by: 1,945
References: 32
Citations by year: 2026: 50 · 2025: 55 · 2024: 66 · 2023: 72 · 2022: 113 · 2021: 111 · 2020: 121 · 2019: 86 · 2018: 98 · 2017: 103 · 2016: 131 · 2015: 137 · 2014: 148 · 2013: 153 · 2012: 162
FWCI: 46.30
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature (publishedVersion)
PubMed (publishedVersion)
RePEc: Research Papers in Economics (submittedVersion)
Funders: U.S. Department of Energy
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Biochemical and biochemical processes
Keywords: isobutanol, n-butanol, 2-phenylethanol, Escherichia coli metabolic engineering, glucose fermentation, biofuels, gasoline substitute, octane number
MeSH terms: Butanols, Bioelectric Energy Sources, Escherichia coli, Fermentation, Genetic Engineering, Glucose

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Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels

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