The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
Abstract
Global energy and environmental problems have stimulated increased efforts towards synthesizing biofuels from renewable resources. Compared to the traditional biofuel, ethanol, higher alcohols offer advantages as gasoline substitutes because of their higher energy density and lower hygroscopicity. In addition, branched-chain alcohols have higher octane numbers compared with their straight-chain counterparts. However, these alcohols cannot be synthesized economically using native organisms. Here we present a metabolic engineering approach using Escherichia coli to produce higher alcohols including isobutanol, 1-butanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from glucose, a renewable carbon source. This strategy uses the host's highly active amino acid biosynthetic pathway and diverts its 2-keto acid intermediates for alcohol synthesis. In particular, we have achieved high-yield, high-specificity production of isobutanol from glucose. The strategy enables the exploration of biofuels beyond those naturally accumulated to high quantities in microbial fermentation.
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“The cyanobacterial cumulative titre of 1247 mg/L over 58 days equates to approximately 21.5 mg L−1 d−1 average daily productivity, compared to approximately 10,900 mg L−1 d−1 for E. coli—a several-hundred-fold difference reflecting light-dependent growth constraints rather than poor pathway design.”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [175]
In the citing paper
“The longer chain alcohols possess higher energy content and can be stored and transported easier than ethanol (Atsumi et al. 2008).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Atsumi et al. 2008
In the citing paper
“The production of longer chain alcohols (C5–C–8) in E. coli bacteria has been achieved by the overproduction of 2-keto acids, intermediates of the amino acid biosynthesis. These intermediates were later converted to butanol derivatives by the heterologous expression of 2-keto acid decarboxylase and alcohol dehydrogenase (Atsumi et al. 2008; Zhang et al. 2008).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Atsumi et al. 2008
In the citing paper
“It is difficult to identify a microorganism suitable to produce C5 and other C4 substituted alcohols from sugar glucose while minimising the steps in the process and making it cost effective [141].”
From Investigation of Biofuel as a Potential Renewable Energy Source (2021) · cites this paper as [141]
Citations
Cites
Cox, B. M., Opheim, K. E., Teschemacher, H., & D, A. G. M. (1975) · Life Sciences · doi:10.1016/0024-3205(75)90272-6 · Cited by 1,708
Lutz, R. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.6.1203 · Cited by 1,694
Engineering Yeast Transcription Machinery for Improved Ethanol Tolerance and Production
Alper, H., Moxley, J., Nevoigt, E., Fink, G. R., & Stephanopoulos, G. (2006) · Science · doi:10.1126/science.1131969 · Cited by 823
Improving lycopene production in Escherichia coli by engineering metabolic control
Farmer, W. R., & Liao, J. C. (2000) · Nature Biotechnology · doi:10.1038/75398 · Cited by 525
Self-citationBalancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli
Pitera, D. J., Paddon, C. J., Newman, J. D., & Keasling, J. D. (2006) · Metabolic Engineering · doi:10.1016/j.ymben.2006.11.002 · Cited by 440
The Catabolism of Amino Acids to Long Chain and Complex Alcohols in Saccharomyces cerevisiae
Dickinson, J. R., Salgado, L. E. J., & Hewlins, M. J. E. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m211914200 · Cited by 375
Enteric Bacterial Catalysts for Fuel Ethanol Production
Ingram, L. O., Aldrich, H. C., Borges, A., Causey, T. B., Martı́nez, A., Martínez‐Morales, F., Saleh, A. Z., Underwood, S. A., Yomano, L. P., York, S. W., et al. (1999) · Biotechnology Progress · doi:10.1021/bp9901062 · Cited by 272
Metabolic engineering for drug discovery and development
Khosla, C., & Keasling, J. D. (2003) · Nature Reviews Drug Discovery · doi:10.1038/nrd1256 · Cited by 208
Vuralhan, Z., de Morais, M. A., Tai, S. L., Piper, M. D. W., & Pronk, J. T. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.8.4534-4541.2003 · Cited by 206
Nucleotide sequence of the yeast alcohol dehydrogenase II gene.
Russell, D. W., Smith, M. J., Williamson, V. M., & Young, E. T. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)32979-x · Cited by 205
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Protein design for pathway engineering
Eriksen, D. T., Lian, J., & Zhao, H. (2013) · Journal of Structural Biology · doi:10.1016/j.jsb.2013.03.011 · Cited by 71
Li, S., Huang, D., Li, Y., Wen, J., & Jia, X. (2012) · Microbial Cell Factories · doi:10.1186/1475-2859-11-101 · Cited by 71
Alternative Approaches for p -Xylene Production from Starch: Techno-Economic Analysis
Lin, Z., Nikolakis, V. A., & Ierapetritou, M. (2014) · Industrial & Engineering Chemistry Research · doi:10.1021/ie402469j · Cited by 71
Mapping the Emergence of Synthetic Biology
Raimbault, B., Cointet, J., & Joly, P. (2016) · PLoS ONE · doi:10.1371/journal.pone.0161522 · Cited by 73
Motagamwala, A. H., Won, W., Maravelias, C. T., & Dumesic, J. A. (2016) · Green Chemistry · doi:10.1039/c6gc02297a · Cited by 71
Blue-Green Algae in Surface Water: Problems and Opportunities
Vu, H. P., Nguyen, L. N., Zdarta, J., Nga, T. T. V., & Nghiem, L. D. (2020) · Current Pollution Reports · doi:10.1007/s40726-020-00140-w · Cited by 72
Biobutanol Production from Acetone–Butanol–Ethanol Fermentation: Developments and Prospects
Lin, Z., Cong, W., & Zhang, J. (2023) · Fermentation · doi:10.3390/fermentation9090847 · Cited by 70
Engineering specialized metabolic pathways—is there a room for enzyme improvements?
Bar‐Even, A., & Tawfik, D. S. (2012) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2012.10.006 · Cited by 69
Algal biofuels in Canada: Status and potential
Scaife, M. A., Merkx-Jacques, A., Woodhall, D. L., & Armenta, R. E. (2015) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2014.12.024 · Cited by 70
Autonomous bacterial localization and gene expression based on nearby cell receptor density
Wu, H., Tsao, C., Quan, D. N., Cheng, Y., Servinsky, M. D., Carter, K. K., Jee, K. J., Terrell, J. L., Zargar, A., Rubloff, G. W., et al. (2013) · Molecular Systems Biology · doi:10.1038/msb.2012.71 · Cited by 69
Engineering butanol‐tolerance in escherichia coli with artificial transcription factor libraries
Lee, J. Y., Yang, K. S., Jang, S. A., Sung, B. H., & Kim, S. C. (2010) · Biotechnology and Bioengineering · doi:10.1002/bit.22989 · Cited by 70
Luan, G., Cai, Z., Li, Y., & Ma, Y. (2013) · Biotechnology for Biofuels · doi:10.1186/1754-6834-6-137 · Cited by 69
Amino Acid Production by Corynebacterium glutamicum
Ikeda, M., & Takeno, S. (2012) · Microbiology monographs · doi:10.1007/978-3-642-29857-8_4 · Cited by 70
Shen, L., Nishimura, Y., Matsuda, F., Ishii, J., & Kondo, A. (2016) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2015.12.022 · Cited by 70
Furfural tolerance and detoxification mechanism in Candida tropicalis
Wang, S., Cheng, G., Joshua, C. J., He, Z., Sun, X., Li, R., Liu, L., & Yuan, Q. (2016) · Biotechnology for Biofuels · doi:10.1186/s13068-016-0668-x · Cited by 70
Lim, H. G., Lee, J. H., Noh, M. H., & Jung, G. Y. (2018) · Journal of Agricultural and Food Chemistry · doi:10.1021/acs.jafc.8b00458 · Cited by 72
Production of bulk chemicals via novel metabolic pathways in microorganisms
Shin, J. H., Kim, H. U., Kim, D. I., & Lee, S. Y. (2012) · Biotechnology Advances · doi:10.1016/j.biotechadv.2012.12.008 · Cited by 69
Wheeldon, I. R., Christopher, P., & Blanch, H. W. (2017) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2017.02.019 · Cited by 70
Jain, R., Sun, X., Yuan, Q., & Yan, Y. (2014) · ACS Synthetic Biology · doi:10.1021/sb500345t · Cited by 69
Fuels and Chemicals from Hemicellulose Sugars
Ji, X., Huang, H., Nie, Z.-K., Qu, L., Xu, Q. C., & Tsao, G. T. N. (2011) · Advances in biochemical engineering, biotechnology · doi:10.1007/10_2011_124 · Cited by 69
Robinson, C. J., Carbonell, P., Jervis, A. J., Yan, C., Hollywood, K. A., Dunstan, M. S., Currin, A., Swainston, N., Spiess, R., Taylor, S., et al. (2020) · Metabolic Engineering · doi:10.1016/j.ymben.2020.04.008 · Cited by 68
Wang, J., Wu, Y., Sun, X., Yuan, Q., & Yan, Y. (2017) · ACS Synthetic Biology · doi:10.1021/acssynbio.7b00136 · Cited by 68
Understanding and application of Bacillus nitrogen regulation: A synthetic biology perspective
He, H., Li, Y., Zhang, L., Ding, Z., & Shi, G. (2022) · Journal of Advanced Research · doi:10.1016/j.jare.2022.09.003 · Cited by 67
Computational evaluation of Synechococcus sp. PCC 7002 metabolism for chemical production
Vu, T. T., Hill, E. A., Kucek, L. A., Konopka, A. E., Beliaev, A. S., & Reed, J. L. (2013) · Biotechnology Journal · doi:10.1002/biot.201200315 · Cited by 67
Which is a better transportation fuel - butanol or ethanol?
Szulczyk, K. (2010) · International journal of energy and environment · Cited by 67
Systems Biology – A Guide for Understanding and Developing Improved Strains of Lactic Acid Bacteria
Liu, J., Chan, S. H. J., Chen, J., Solem, C., & Jensen, P. R. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.00876 · Cited by 68
Enabling technology and core theory of synthetic biology
Zhang, X., Liu, C., Dai, J., Yuan, Y., Gao, C., Feng, Y. F., Wu, B., Wei, P., You, C., Wang, X., et al. (2023) · Science China Life Sciences · doi:10.1007/s11427-022-2214-2 · Cited by 69
Enzymatic Decarboxylation—An Emerging Reaction for Chemicals Production from Renewable Resources
Kourist, R., Guterl, J., Miyamoto, K., & Sieber, V. (2014) · ChemCatChem · doi:10.1002/cctc.201300881 · Cited by 67
Two-dimensional isobutyl acetate production pathways to improve carbon yield
Tashiro, Y., Desai, S. H., & Atsumi, S. (2015) · Nature Communications · doi:10.1038/ncomms8488 · Cited by 66
Self-citationMatsumoto, T., Tanaka, T., & Kondo, A. (2017) · Bioresource Technology · doi:10.1016/j.biortech.2017.05.008 · Cited by 66
Li, Z., Qiu, L., Cheng, X., Li, Y., & Wu, H. (2017) · Fuel · doi:10.1016/j.fuel.2017.09.036 · Cited by 66
Dhamankar, H., & Prather, K. L. J. (2011) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2011.05.001 · Cited by 65
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Zhang, J., Wei, L., Man, X., Jiang, X. D., Zhang, Y., Hu, E., & Huang, Z. (2012) · Energy & Fuels · doi:10.1021/ef3005042 · Cited by 65
Zhang, S., Liu, Y., & Bryant, D. A. (2015) · Metabolic Engineering · doi:10.1016/j.ymben.2015.10.001 · Cited by 65
Muniraj, I. K., Uthandi, S., Hu, Z., Xiao, L., & Zhan, X. (2015) · Environmental Technology Reviews · doi:10.1080/21622515.2015.1018340 · Cited by 64
Barajas, J. F., Phelan, R. M., Schaub, A. J., Kliewer, J. T., Kelly, P. J., Jackson, D. R., Luo, R., Keasling, J. D., & Tsai, S. (2015) · Chemistry & Biology · doi:10.1016/j.chembiol.2015.06.022 · Cited by 65
Recent trends in metabolic engineering of microorganisms for the production of advanced biofuels
Cheon, S., Kim, H. M., Gustavsson, M., & Lee, S. Y. (2016) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2016.08.003 · Cited by 65
Hellingwerf, K. J., & Teixeira de Mattos, M. J. (2009) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2009.02.002 · Cited by 64
Chemocatalytic upgrading of tailored fermentation products toward biodiesel
Sreekumar, S., Baer, Z. C., Gross, E., Padmanaban, S., Goulas, K., Gunbas, G., Alayoglu, S., Blanch, H. W., Clark, D. S., & Toste, F. D. (2014) · ChemSusChem · doi:10.1002/cssc.201402244 · Cited by 64
Comparative analysis of the chemical and biochemical synthesis of keto acids
Luo, Z., Yu, S., Zeng, W., & Zhou, J. (2021) · Biotechnology Advances · doi:10.1016/j.biotechadv.2021.107706 · Cited by 65
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Koide, T., Pang, W. L., & Baliga, N. S. (2009) · Nature Reviews Microbiology · doi:10.1038/nrmicro2107 · Cited by 65
Zhang, H., Chong, H., Ching, C. B., Song, H., & Jiang, R. (2012) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-012-4012-5 · Cited by 63
Evolutionary Engineering Improves Tolerance for Replacement Jet Fuels in Saccharomyces cerevisiae
Brennan, T. C. R., Williams, T. C., Schulz, B. L., Palfreyman, R. W., Krömer, J. O., & Nielsen, L. K. (2015) · Applied and Environmental Microbiology · doi:10.1128/aem.04144-14 · Cited by 64
McNerney, M. P., Watstein, D. M., & Styczynski, M. P. (2015) · Metabolic Engineering · doi:10.1016/j.ymben.2015.06.011 · Cited by 64
Priority-based multiple products from microalgae: review on techniques and strategies
Sarkar, S., Manna, M. S., Bhowmick, T. K., & Gayen, K. (2020) · Critical Reviews in Biotechnology · doi:10.1080/07388551.2020.1753649 · Cited by 64
Bioengineered microbial platforms for biomass-derived biofuel production – A review
Lu, H., Yadav, V., Zhong, M., Bilal, M., Taherzadeh, M. J., & Iqbal, H. M. N. (2021) · Chemosphere · doi:10.1016/j.chemosphere.2021.132528 · Cited by 63
Pathway and protein engineering approaches to produce novel and commodity small molecules
Bhan, N., Xu, P., & Koffas, M. A. G. (2013) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2013.02.019 · Cited by 63
Synthetic Genomics and Synthetic Biology Applications Between Hopes and Concerns
König, H., Frank, D. O., Heil, R., & Coenen, C. (2013) · Current Genomics · doi:10.2174/1389202911314010003 · Cited by 64
Ensemble modeling for strain development of l-lysine-producing Escherichia coli
Contador, C. A., Rizk, M. L., Asenjo, J. A., & Liao, J. C. (2009) · Metabolic Engineering · doi:10.1016/j.ymben.2009.04.002 · Cited by 63
Self-citationSoma, Y., Inokuma, K., Tanaka, T., Ogino, C., Kondo, A., Okamoto, M., & Hanai, T. (2012) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2012.02.019 · Cited by 63
Self-citationHwang, J., Park, J., Seo, J., Cha, M., Cho, B., Kim, J., & Kim, B. (2008) · Biotechnology and Bioengineering · doi:10.1002/bit.22178 · Cited by 63
Relative Reactivities of the Isomeric Butanols and Ethanol in an Ignition Quality Tester
Haas, F. M., Ramcharan, A. M., & Dryer, F. L. (2011) · Energy & Fuels · doi:10.1021/ef2008024 · Cited by 63
Wang, J., Shen, X., Jain, R., Wang, J., Yuan, Q., & Yan, Y. (2017) · Metabolic Engineering · doi:10.1016/j.ymben.2017.03.003 · Cited by 63
Liu, X., Xie, H., Roussou, S., & Lindblad, P. (2022) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2021.07.014 · Cited by 62
Ida, Y., Furusawa, C., Hirasawa, T., & Shimizu, H. (2011) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2011.09.019 · Cited by 62
Guo, D., Zhu, J., Deng, Z., & Liu, T. (2014) · Metabolic Engineering · doi:10.1016/j.ymben.2014.01.003 · Cited by 62
The imminent role of protein engineering in synthetic biology
Foo, J. L., Ching, C. B., Chang, M. W., & Leong, S. S. J. (2011) · Biotechnology Advances · doi:10.1016/j.biotechadv.2011.09.008 · Cited by 65
Chong, H., Geng, H., Zhang, H., Song, H., Huang, L., & Jiang, R. (2013) · Biotechnology and Bioengineering · doi:10.1002/bit.25134 · Cited by 62
McKee, A. E., Rutherford, B. J., Chivian, D. C., Baidoo, E., Juminaga, D., Kuo, D., Benke, P. I., Dietrich, J. A., Ma, S. M., Arkin, A. P., et al. (2012) · Microbial Cell Factories · doi:10.1186/1475-2859-11-79 · Cited by 62
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Lopes, M. S. G. (2015) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-015-1606-9 · Cited by 63
Nour, M., Sun, Z., EL‐Seesy, A. I., & Li, X. (2021) · Fuel · doi:10.1016/j.fuel.2021.121144 · Cited by 61
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Pan, H., Wang, J., Wu, H., Li, Z., & Lian, J. (2021) · Biotechnology for Biofuels · doi:10.1186/s13068-021-02063-0 · Cited by 61
Development of microbial cell factories for bio-refinery through synthetic bioengineering
Kondo, A., Ishii, J., Hara, K. Y., Hasunuma, T., & Matsuda, F. (2012) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2012.05.021 · Cited by 62
Synthetic biology and biomass conversion: a match made in heaven?
French, C. E. (2009) · Journal of The Royal Society Interface · doi:10.1098/rsif.2008.0527.focus · Cited by 61
Biological Production of 2‐Butanone in Escherichia coli
Yoneda, H., Tantillo, D. J., & Atsumi, S. (2013) · ChemSusChem · doi:10.1002/cssc.201300853 · Cited by 63
Self-citationTao, H., Guo, D., Zhang, Y., Deng, Z., & Liu, T. (2015) · Biotechnology for Biofuels · doi:10.1186/s13068-015-0270-7 · Cited by 61
Yuan, J., & Ching, C. B. (2014) · ACS Synthetic Biology · doi:10.1021/sb500079f · Cited by 61
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Lippow, S. M., Moon, T. S., Basu, S., Yoon, S., Li, X., Chapman, B., Robison, K. E., Lipovšek, D., & Prather, K. L. J. (2010) · Chemistry & Biology · doi:10.1016/j.chembiol.2010.10.012 · Cited by 60
Isobutanol production from cellobionic acid in Escherichia coli
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Self-citationToward nitrogen neutral biofuel production
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Self-citationNielsen, D. R., Amarasiriwardena, G. S., & Prather, K. L. J. (2009) · Bioresource Technology · doi:10.1016/j.biortech.2009.12.003 · Cited by 60
Metabolic engineering of strains: from industrial-scale to lab-scale chemical production
Sun, J., & Alper, H. S. (2014) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-014-1539-8 · Cited by 60
Sarma, M. K., Kaushik, S., & Goswami, P. (2016) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2016.03.043 · Cited by 62
Reaction Design for the Compartmented Combination of Heterogeneous and Enzyme Catalysis
Sperl, J., Carsten, J. M., Guterl, J., Lommes, P., & Sieber, V. (2016) · ACS Catalysis · doi:10.1021/acscatal.6b01276 · Cited by 60
Zhang, Y., Lane, S., Chen, J., Hammer, S. K., Luttinger, J., Yang, L., Jin, Y., & Avalos, J. L. (2019) · Biotechnology for Biofuels · doi:10.1186/s13068-019-1560-2 · Cited by 60
Zareian, M., Böhner, N., Loos, H. M., Silcock, P. J., Bremer, P. J., & Beauchamp, J. D. (2018) · Food Packaging and Shelf Life · doi:10.1016/j.fpsl.2018.08.001 · Cited by 60
Biotechnology and biofuels: paving the way towards a sustainable and equitable energy for the future
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De-novo synthesis of 2-phenylethanol by Enterobactersp. CGMCC 5087
Zhang, H., Cao, M., Jiang, X., Zou, H., Wang, C., Xu, X., & Xian, M. (2014) · BMC Biotechnology · doi:10.1186/1472-6750-14-30 · Cited by 59
Wu, X., Huang, Z., Jin, C., Wang, X., & Wei, L. (2010) · Combustion Science and Technology · doi:10.1080/00102202.2010.516037 · Cited by 59
Digital and analog gene circuits for biotechnology
Roquet, N., & Lu, T. K. (2014) · Biotechnology Journal · doi:10.1002/biot.201300258 · Cited by 58
Recent progress in synthetic biology for microbial production of C3–C10 alcohols
Lamsen, E. N., & Atsumi, S. (2012) · Frontiers in Microbiology · doi:10.3389/fmicb.2012.00196 · Cited by 59
Self-citationSong, Z., Huang, Y., Xu, W. L., Wang, L., Bao, Y., Li, S., & Yu, M. (2015) · Scientific Reports · doi:10.1038/srep13981 · Cited by 62
Biosynthesis of floral scent 2-phenylethanol in rose flowers
Hirata, H., Ohnishi, T., & Watanabe, N. (2016) · Bioscience Biotechnology and Biochemistry · doi:10.1080/09168451.2016.1191333 · Cited by 59
Modular pathway engineering for the microbial production of branched-chain fatty alcohols
Jiang, W. J., Qiao, J. B., Bentley, G. J., Liu, D., & Zhang, F. (2017) · Biotechnology for Biofuels · doi:10.1186/s13068-017-0936-4 · Cited by 59
Production of fuels and chemicals from renewable resources using engineered Escherichia coli
Zhao, C., Zhang, Y., & Li, Y. (2019) · Biotechnology Advances · doi:10.1016/j.biotechadv.2019.06.001 · Cited by 59
Advances in Synthetic Biology and Biosafety Governance
Li, J., Zhao, H., Zheng, L., & An, W. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.598087 · Cited by 59
Effect of Formic Acid on Conversion of Fructose to 5-Hydroxymethylfurfural in Aqueous/Butanol Media
Jiang, N., Huang, R., Qi, W. D., Su, R., & He, Z. (2011) · BioEnergy Research · doi:10.1007/s12155-011-9141-7 · Cited by 58
Lin, X., Li, R., Wen, Q., Wu, J., Fan, J., Jin, X., Qian, W., Liu, D., Chen, X., Chen, Y., et al. (2013) · Biotechnology and Bioprocess Engineering · doi:10.1007/s12257-012-0549-5 · Cited by 58
Ninh, P. H., Honda, K., Sakai, T., Okano, K., & Ohtake, H. (2014) · Biotechnology and Bioengineering · doi:10.1002/bit.25338 · Cited by 58
Holistic bioengineering: rewiring central metabolism for enhanced bioproduction
Aslan, S., Noor, Ε., & Bar‐Even, A. (2017) · Biochemical Journal · doi:10.1042/bcj20170377 · Cited by 56
Comparing proteins and nucleic acids for next-generation biomolecular engineering
Pugh, G., Burns, J. R., & Howorka, S. (2018) · Nature Reviews Chemistry · doi:10.1038/s41570-018-0015-9 · Cited by 59
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Bai, W., Geng, W., Wang, S., & Zhang, F. (2019) · Biotechnology for Biofuels · doi:10.1186/s13068-019-1424-9 · Cited by 58
Escher-FBA: a web application for interactive flux balance analysis
Rowe, E., Palsson, B. Ø., & King, Z. A. (2018) · BMC Systems Biology · doi:10.1186/s12918-018-0607-5 · Cited by 58
Microbial 2-butanol production with Lactobacillus diolivorans
Rußmayer, H., Marx, H., & Sauer, M. (2019) · Biotechnology for Biofuels · doi:10.1186/s13068-019-1594-5 · Cited by 58
Ameen, S., Ahmad, M. Z., Mohsin, M., Qureshi, M. I., Ibrahim, M. M., Abdin, M. Z., & Ahmad, A. (2016) · Journal of Nanobiotechnology · doi:10.1186/s12951-016-0204-y · Cited by 57
Lee, S.-H., Yun, E. J., Kim, J., Lee, S. J., Um, Y., & Kim, K. H. (2016) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-016-7760-9 · Cited by 58
Mazzoli, R., & Olson, D. G. (2020) · Advances in applied microbiology · doi:10.1016/bs.aambs.2020.07.004 · Cited by 57
Guo, S., Asset, T., & Atanassov, P. B. (2021) · ACS Catalysis · doi:10.1021/acscatal.0c04862 · Cited by 61
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Metadata
- Authors
- Atsumi, S., Hanai, T., & Liao, J. C.
- Affiliations
- University of California, Los Angeles (US)
- Corresponding author
- Liao, James C.
- Venue
- Nature
- Published
- 2008-01
- Volume
- 451
- Issue
- 7174
- Pages
- 86-89
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1978119727
- Cited by
- 1,945
- References
- 32
- Citations by year
- 2026: 50 · 2025: 55 · 2024: 66 · 2023: 72 · 2022: 113 · 2021: 111 · 2020: 121 · 2019: 86 · 2018: 98 · 2017: 103 · 2016: 131 · 2015: 137 · 2014: 148 · 2013: 153 · 2012: 162
- FWCI
- 46.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- U.S. Department of Energy
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Biochemical and biochemical processes
- Keywords
- isobutanol, n-butanol, 2-phenylethanol, Escherichia coli metabolic engineering, glucose fermentation, biofuels, gasoline substitute, octane number
- MeSH terms
- Butanols, Bioelectric Energy Sources, Escherichia coli, Fermentation, Genetic Engineering, Glucose
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