The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
Abstract
Global energy and environmental problems have stimulated increased efforts towards synthesizing biofuels from renewable resources. Compared to the traditional biofuel, ethanol, higher alcohols offer advantages as gasoline substitutes because of their higher energy density and lower hygroscopicity. In addition, branched-chain alcohols have higher octane numbers compared with their straight-chain counterparts. However, these alcohols cannot be synthesized economically using native organisms. Here we present a metabolic engineering approach using Escherichia coli to produce higher alcohols including isobutanol, 1-butanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from glucose, a renewable carbon source. This strategy uses the host's highly active amino acid biosynthetic pathway and diverts its 2-keto acid intermediates for alcohol synthesis. In particular, we have achieved high-yield, high-specificity production of isobutanol from glucose. The strategy enables the exploration of biofuels beyond those naturally accumulated to high quantities in microbial fermentation.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“The cyanobacterial cumulative titre of 1247 mg/L over 58 days equates to approximately 21.5 mg L−1 d−1 average daily productivity, compared to approximately 10,900 mg L−1 d−1 for E. coli—a several-hundred-fold difference reflecting light-dependent growth constraints rather than poor pathway design.”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [175]
In the citing paper
“The longer chain alcohols possess higher energy content and can be stored and transported easier than ethanol (Atsumi et al. 2008).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Atsumi et al. 2008
In the citing paper
“The production of longer chain alcohols (C5–C–8) in E. coli bacteria has been achieved by the overproduction of 2-keto acids, intermediates of the amino acid biosynthesis. These intermediates were later converted to butanol derivatives by the heterologous expression of 2-keto acid decarboxylase and alcohol dehydrogenase (Atsumi et al. 2008; Zhang et al. 2008).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Atsumi et al. 2008
In the citing paper
“It is difficult to identify a microorganism suitable to produce C5 and other C4 substituted alcohols from sugar glucose while minimising the steps in the process and making it cost effective [141].”
From Investigation of Biofuel as a Potential Renewable Energy Source (2021) · cites this paper as [141]
Citations
Cites
Cox, B. M., Opheim, K. E., Teschemacher, H., & D, A. G. M. (1975) · Life Sciences · doi:10.1016/0024-3205(75)90272-6 · Cited by 1,708
Lutz, R. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.6.1203 · Cited by 1,694
Engineering Yeast Transcription Machinery for Improved Ethanol Tolerance and Production
Alper, H., Moxley, J., Nevoigt, E., Fink, G. R., & Stephanopoulos, G. (2006) · Science · doi:10.1126/science.1131969 · Cited by 823
Improving lycopene production in Escherichia coli by engineering metabolic control
Farmer, W. R., & Liao, J. C. (2000) · Nature Biotechnology · doi:10.1038/75398 · Cited by 525
Self-citationBalancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli
Pitera, D. J., Paddon, C. J., Newman, J. D., & Keasling, J. D. (2006) · Metabolic Engineering · doi:10.1016/j.ymben.2006.11.002 · Cited by 440
The Catabolism of Amino Acids to Long Chain and Complex Alcohols in Saccharomyces cerevisiae
Dickinson, J. R., Salgado, L. E. J., & Hewlins, M. J. E. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m211914200 · Cited by 375
Enteric Bacterial Catalysts for Fuel Ethanol Production
Ingram, L. O., Aldrich, H. C., Borges, A., Causey, T. B., Martı́nez, A., Martínez‐Morales, F., Saleh, A. Z., Underwood, S. A., Yomano, L. P., York, S. W., et al. (1999) · Biotechnology Progress · doi:10.1021/bp9901062 · Cited by 272
Metabolic engineering for drug discovery and development
Khosla, C., & Keasling, J. D. (2003) · Nature Reviews Drug Discovery · doi:10.1038/nrd1256 · Cited by 208
Vuralhan, Z., de Morais, M. A., Tai, S. L., Piper, M. D. W., & Pronk, J. T. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.8.4534-4541.2003 · Cited by 206
Nucleotide sequence of the yeast alcohol dehydrogenase II gene.
Russell, D. W., Smith, M. J., Williamson, V. M., & Young, E. T. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)32979-x · Cited by 205
Cited by
Sorted by citations
Items 101–200 of 1,004
Designing cell function: assembly of synthetic gene circuits for cell biology applications
Xie, M., & Fussenegger, M. (2018) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-018-0024-z · Cited by 304
Advances on waste valorization: new horizons for a more sustainable society
Arancon, R. A. D., Lin, C. S. K., Chan, K. M., Kwan, T. H., & Luque, R. (2013) · Energy Science & Engineering · doi:10.1002/ese3.9 · Cited by 291
Ren, D., Wong, N. T., Handoko, A. D., Huang, Y., & Yeo, B. S. (2015) · The Journal of Physical Chemistry Letters · doi:10.1021/acs.jpclett.5b02554 · Cited by 283
Pfromm, P. H., Amanor‐Boadu, V., Nelson, R., Vadlani, P. V., & Madl, R. L. (2010) · Biomass and Bioenergy · doi:10.1016/j.biombioe.2009.12.017 · Cited by 277
Adaptive laboratory evolution principles and applications in industrial biotechnology
Mavrommati, M., Daskalaki, A., Papanikolaou, S., & Aggelis, G. (2021) · Biotechnology Advances · doi:10.1016/j.biotechadv.2021.107795 · Cited by 272
Sainsbury, P. D., Hardiman, E. M., Ahmad, M., Otani, H., Seghezzi, N., Eltis, L. D., & Bugg, T. D. H. (2013) · ACS Chemical Biology · doi:10.1021/cb400505a · Cited by 279
Cyanobacterial biofuel production
Machado, I. M. P., & Atsumi, S. (2012) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2012.03.005 · Cited by 271
Self-citationToward low-cost biological and hybrid biological/catalytic conversion of cellulosic biomass to fuels
Lynd, L. R., Beckham, G. T., Guss, A. M., Jayakody, L. N., Karp, E. M., Maranas, C. D., McCormick, R. L., Amador‐Noguez, D., Bomble, Y. J., Davison, B. H., et al. (2022) · Energy & Environmental Science · doi:10.1039/d1ee02540f · Cited by 268
ePathBrick: A Synthetic Biology Platform for Engineering Metabolic Pathways in E. coli
Xu, P., Vansiri, A., Bhan, N., & Koffas, M. A. G. (2012) · ACS Synthetic Biology · doi:10.1021/sb300016b · Cited by 258
Second‐generation biofuels and local bioenergy systems
Antízar-Ladislao, B., & Turrion-Gomez, J. L. (2008) · Biofuels Bioproducts and Biorefining · doi:10.1002/bbb.97 · Cited by 257
Baez, A., Cho, K.-M., & Liao, J. C. (2011) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-011-3173-y · Cited by 255
Self-citationIntegrating Biological Redesign: Where Synthetic Biology Came From and Where It Needs to Go
Way, J. C., Collins, J. J., Keasling, J. D., & Silver, P. A. (2014) · Cell · doi:10.1016/j.cell.2014.02.039 · Cited by 259
Adegboye, M. F., Ojuederie, O. B., Talia, P. M., & Babalola, O. O. (2021) · Biotechnology for Biofuels · doi:10.1186/s13068-020-01853-2 · Cited by 267
Cell‐Free Metabolic Engineering: Production of Chemicals by Minimized Reaction Cascades
Guterl, J., Garbe, D., Carsten, J. M., Steffler, F., Sommer, B., Reiße, S., Philipp, A., Haack, M., Rühmann, B., Koltermann, A., et al. (2012) · ChemSusChem · doi:10.1002/cssc.201200365 · Cited by 251
Biofuel production in Escherichia coli: the role of metabolic engineering and synthetic biology
Clomburg, J. M., & González, R. (2010) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-010-2446-1 · Cited by 250
Atsumi, S., & Liao, J. C. (2008) · Applied and Environmental Microbiology · doi:10.1128/aem.02046-08 · Cited by 249
Self-citationEngineering microbial factories for synthesis of value-added products
Du, J., Shao, Z., & Zhao, H. (2011) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-011-0970-3 · Cited by 253
Jiang, Y., Xu, C., Dong, F., Yang, Y., Jiang, W., & Yang, S. (2009) · Metabolic Engineering · doi:10.1016/j.ymben.2009.06.002 · Cited by 247
Recent advances in systems metabolic engineering tools and strategies
Chae, T. U., Choi, S. Y., Kim, J. W., Ko, Y., & Lee, S. Y. (2017) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2017.06.007 · Cited by 251
Sustainable oil palm industry: The possibilities
Khatun, R., Reza, M. I. H., Moniruzzaman, M., & Yaakob, Z. (2017) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2017.03.077 · Cited by 276
Metabolic engineering of Pichia pastoris for production of isobutanol and isobutyl acetate
Siripong, W., Wolf, P., Kusumoputri, T. P., Downes, J. J., Kocharin, K., Tanapongpipat, S., & Runguphan, W. (2018) · Biotechnology for Biofuels · doi:10.1186/s13068-017-1003-x · Cited by 242
Tao, L., Tan, E. C. D., McCormick, R., Zhang, M., Aden, A., He, X., & Zigler, B. T. (2014) · Biofuels, Bioproducts and Biorefining · doi:10.1002/bbb.1431 · Cited by 241
Synthesis of Methyl Halides from Biomass Using Engineered Microbes
Bayer, T. S., Widmaier, D. M., Temme, K., Mirsky, E. A., Santi, D. V., & Voigt, C. A. (2009) · Journal of the American Chemical Society · doi:10.1021/ja809461u · Cited by 241
Lennen, R. M., Braden, D. J., West, R. M., Dumesic, J. A., & Pfleger, B. F. (2010) · Biotechnology and Bioengineering · doi:10.1002/bit.22660 · Cited by 238
How well can renewable resources mimic commodity monomers and polymers?
Mathers, R. T. (2011) · Journal of Polymer Science Part A Polymer Chemistry · doi:10.1002/pola.24939 · Cited by 246
Customized optimization of metabolic pathways by combinatorial transcriptional engineering
Du, J., Yuan, Y., Si, T., Lian, J., & Zhao, H. (2012) · Nucleic Acids Research · doi:10.1093/nar/gks549 · Cited by 239
Dynamic balancing of intestinal short-chain fatty acids: The crucial role of bacterial metabolism
Xu, Y., Zhu, Y., Li, X., & Sun, B. (2020) · Trends in Food Science & Technology · doi:10.1016/j.tifs.2020.02.026 · Cited by 239
Computational tools for the synthetic design of biochemical pathways
Medema, M. H., van Raaphorst, R., Takano, E., & Breitling, R. (2012) · Nature Reviews Microbiology · doi:10.1038/nrmicro2717 · Cited by 230
Chen, X., Zhou, L., Tian, K., Kumar, A., Singh, S., Prior, B. A., & Wang, Z. (2013) · Biotechnology Advances · doi:10.1016/j.biotechadv.2013.02.009 · Cited by 236
Dong, T., Knoshaug, E. P., Davis, R., Laurens, L. M. L., Van Wychen, S., Pienkos, P. T., & Nagle, N. (2016) · Algal Research · doi:10.1016/j.algal.2015.12.021 · Cited by 224
The Impact of Microalgae in Food Science and Technology
Matos, Â. P. (2017) · Journal of the American Oil Chemists Society · doi:10.1007/s11746-017-3050-7 · Cited by 222
López‐Gallego, F., & Schmidt‐Dannert, C. (2009) · Current Opinion in Chemical Biology · doi:10.1016/j.cbpa.2009.11.023 · Cited by 219
Metabolic engineering of Escherichia coli for the production of cadaverine: A five carbon diamine
Qian, Z., Xia, X., & Lee, S. Y. (2010) · Biotechnology and Bioengineering · doi:10.1002/bit.22918 · Cited by 220
Oßwald, P., Güldenberg, H., Kohse‐Höinghaus, K., Yang, B., Yuan, T., & Qi, F. (2010) · Combustion and Flame · doi:10.1016/j.combustflame.2010.06.003 · Cited by 217
Kim, J., & Reed, J. L. (2010) · BMC Systems Biology · doi:10.1186/1752-0509-4-53 · Cited by 218
Chen, X., Nielsen, K. F., Borodina, I., Kielland‐Brandt, M. C., & Karhumaa, K. (2011) · Biotechnology for Biofuels · doi:10.1186/1754-6834-4-21 · Cited by 216
Beckham, G. T., Matthews, J. F., Peters, B., Bomble, Y. J., Himmel, M. E., & Crowley, M. F. (2011) · The Journal of Physical Chemistry B · doi:10.1021/jp1106394 · Cited by 212
Expanding ester biosynthesis in Escherichia coli
Rodriguez, G. M., Tashiro, Y., & Atsumi, S. (2014) · Nature Chemical Biology · doi:10.1038/nchembio.1476 · Cited by 215
Self-citationEngineering metabolism through dynamic control
Venayak, N., Anesiadis, N., Cluett, W. R., & Mahadevan, R. (2015) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2014.12.022 · Cited by 211
Valorization of greenhouse carbon dioxide emissions into value-added products by catalytic processes
Olajire, A. A. (2013) · Journal of CO2 Utilization · doi:10.1016/j.jcou.2013.10.004 · Cited by 222
Butanol production by Clostridium beijerinckii ATCC 55025 from wheat bran
Liu, Z., Yu, Y., Li, F., Ma, C., & Xu, P. (2010) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-010-0695-8 · Cited by 210
Application of synthetic biology in cyanobacteria and algae
Wang, B., Wang, J., Zhang, W., & Meldrum, D. R. (2012) · Frontiers in Microbiology · doi:10.3389/fmicb.2012.00344 · Cited by 211
Biosolutions to the energy problem
Demain, A. L. (2009) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-008-0521-8 · Cited by 211
Metabolic Engineering: Methodologies and Applications
Volk, M., Tran, V. G., Tan, S., Mishra, S., Fatma, Z., Boob, A. G., Li, H., Xue, P., Martin, T. A., & Zhao, H. (2022) · Chemical Reviews · doi:10.1021/acs.chemrev.2c00403 · Cited by 217
Zymomonas mobilis as a model system for production of biofuels and biochemicals
Yang, S., Fei, Q., Zhang, Y., Contreras, L. M., Utturkar, S. M., Brown, S. D., Himmel, M. E., & Zhang, M. (2016) · Microbial Biotechnology · doi:10.1111/1751-7915.12408 · Cited by 204
Biomanufacturing: history and perspective
Zhang, Y. ‐. P., Sun, J., & Ma, Y. (2016) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-016-1863-2 · Cited by 224
Zymomonas mobilis: a novel platform for future biorefineries
He, M., Wu, B., Qin, H., Ruan, Z., Tan, F., Wang, J., Shui, Z., Dai, L., Zhu, Q., Pan, K., et al. (2014) · Biotechnology for Biofuels · doi:10.1186/1754-6834-7-101 · Cited by 203
Dietrich, J. A., Shis, D. L., Alikhani, A., & Keasling, J. D. (2012) · ACS Synthetic Biology · doi:10.1021/sb300091d · Cited by 206
Scalable production of mechanically tunable block polymers from sugar
Xiong, M., Schneiderman, D. K., Bates, F. S., Hillmyer, M. A., & Zhang, K. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1404596111 · Cited by 203
The path to next generation biofuels: successes and challenges in the era of synthetic biology
Dellomonaco, C., Fava, F., & González, R. (2010) · Microbial Cell Factories · doi:10.1186/1475-2859-9-3 · Cited by 202
Vapor Pressures of Alcohol−Gasoline Blends
Andersen, V. F., Anderson, J. E., Wallington, T. J., Mueller, S. A., & Nielsen, O. J. (2010) · Energy & Fuels · doi:10.1021/ef100254w · Cited by 201
Quantitative analysis and engineering of fatty acid biosynthesis in E. coli
Liu, T., Vora, H., & Khosla, C. (2010) · Metabolic Engineering · doi:10.1016/j.ymben.2010.02.003 · Cited by 203
An integrated network approach identifies the isobutanol response network of Escherichia coli
Brynildsen, M. P., & Liao, J. C. (2009) · Molecular Systems Biology · doi:10.1038/msb.2009.34 · Cited by 201
Self-citationMicrobial synthesis of n-butanol, isobutanol, and other higher alcohols from diverse resources
Lan, E. I., & Liao, J. C. (2012) · Bioresource Technology · doi:10.1016/j.biortech.2012.09.104 · Cited by 201
Self-citationCO2 fixation by anaerobic non-photosynthetic mixotrophy for improved carbon conversion
Jones, S. W., Fast, A. G., Carlson, E. D., Wiedel, C. A., Au, J., Antoniewicz, M. R., Papoutsakis, E. T., & Tracy, B. P. (2016) · Nature Communications · doi:10.1038/ncomms12800 · Cited by 192
Expanding the Fluorine Chemistry of Living Systems Using Engineered Polyketide Synthase Pathways
Walker, M. C., Thuronyi, B. W., Charkoudian, L. K., Lowry, B., Khosla, C., & Chang, M. C. Y. (2013) · Science · doi:10.1126/science.1242345 · Cited by 198
Lau, N., Matsui, M., & Abdullah, A. A. (2015) · BioMed Research International · doi:10.1155/2015/754934 · Cited by 213
Systems metabolic engineering for chemicals and materials
Lee, J. W., Kim, T. Y., Jang, Y., Choi, S., & Lee, S. Y. (2011) · Trends in biotechnology · doi:10.1016/j.tibtech.2011.04.001 · Cited by 194
Recent progress in the development of biofuel 2,5-dimethylfuran
Qian, Y., Zhu, L., Wang, Y., & Lü, X. (2014) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2014.08.085 · Cited by 193
Microbial production of advanced transportation fuels in non-natural hosts
Connor, M. R., & Liao, J. C. (2009) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2009.04.002 · Cited by 193
Self-citationChen, X., Gao, C., Guo, L., Hu, G., Luo, Q., Liu, J., Nielsen, J., Chen, J., & Liu, L. (2017) · Chemical Reviews · doi:10.1021/acs.chemrev.6b00804 · Cited by 191
Biosensors and their applications in microbial metabolic engineering
Zhang, F., & Keasling, J. D. (2011) · Trends in Microbiology · doi:10.1016/j.tim.2011.05.003 · Cited by 192
Panahi, H. K. S., Dehhaghi, M., Kinder, J. E., & Ezeji, T. C. (2019) · Biofuel Research Journal · doi:10.18331/brj2019.6.3.2 · Cited by 191
Zhang, Y. ‐. P. (2014) · Biotechnology Advances · doi:10.1016/j.biotechadv.2014.10.009 · Cited by 193
Biobutanol: the outlook of an academic and industrialist
Bankar, S. B., Survase, S. A., Ojamo, H., & Granström, T. B. (2013) · RSC Advances · doi:10.1039/c3ra43011a · Cited by 193
Metabolic engineering of microorganisms for biofuel production
Majidian, P., Tabatabaei, M., Zeinolabedini, M., Naghshbandi, M. P., & Chisti, Y. (2017) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2017.10.085 · Cited by 195
Dien, S. V. (2013) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2013.03.002 · Cited by 184
Isoprenoid drugs, biofuels, and Chemicals–Artemisinin, farnesene, and beyond
George, K. W., Alonso-Gutierrez, J., Keasling, J. D., & Lee, T. S. (2015) · Advances in biochemical engineering, biotechnology · doi:10.1007/10_2014_288 · Cited by 185
Minty, J. J., Lesnefsky, A. A., Lin, F., Chen, Y., Zaroff, T. A., Veloso, A. B., Xie, B., McConnell, C. A., Ward, R. J., Schwartz, D. R., et al. (2011) · Microbial Cell Factories · doi:10.1186/1475-2859-10-18 · Cited by 185
Zhang, Y. ‐. P. (2009) · Biotechnology and Bioengineering · doi:10.1002/bit.22630 · Cited by 184
Feasibilities of consolidated bioprocessing microbes: From pretreatment to biofuel production
Parisutham, V., Kim, T. H., & Lee, S. K. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2014.03.114 · Cited by 189
Ledesma‐Amaro, R., Dulermo, R., Niehus, X., & Nicaud, J. (2016) · Metabolic Engineering · doi:10.1016/j.ymben.2016.06.004 · Cited by 183
Consolidated bioprocessing of cellulose to isobutanol using Clostridium thermocellum
Lin, P. P.-C., Mi, L., Morioka, A. H., Yoshino, K. M., Konishi, S., Xu, S. C., Papanek, B., Riley, L. A., Guss, A. M., & Liao, J. C. (2015) · Metabolic Engineering · doi:10.1016/j.ymben.2015.07.001 · Cited by 181
Self-citationInokuma, K., Liao, J. C., Okamoto, M., & Hanai, T. (2010) · Journal of Bioscience and Bioengineering · doi:10.1016/j.jbiosc.2010.07.010 · Cited by 180
Self-citationSadhukhan, J., Lloyd, J. R., Scott, K., Premier, G. C., Yu, E. H., Curtis, T. P., & Head, I. M. (2015) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2015.11.015 · Cited by 179
Experimental and modeling study of the auto-ignition of n-heptane/n-butanol mixtures
Zhang, J., Niu, S., Zhang, Y., Tang, C., Jiang, X. D., Hu, E., & Huang, Z. (2012) · Combustion and Flame · doi:10.1016/j.combustflame.2012.09.006 · Cited by 179
Relative potential of biosynthetic pathways for biofuels and bio-based products
Dugar, D., & Stephanopoulos, G. N. (2011) · Nature Biotechnology · doi:10.1038/nbt.2055 · Cited by 179
Rapid and Efficient One-Step Metabolic Pathway Integration in E. coli
Bassalo, M. C., Garst, A. D., Halweg‐Edwards, A. L., Grau, W. C., Domaille, D. W., Mutalik, V. K., Arkin, A. P., & Gill, R. T. (2016) · ACS Synthetic Biology · doi:10.1021/acssynbio.5b00187 · Cited by 179
Metabolic Engineering of Synechocystis sp. Strain PCC 6803 for Isobutanol Production
Varman, A. M., Xiao, Y., Pakrasi, H. B., & Tang, Y. (2012) · Applied and Environmental Microbiology · doi:10.1128/aem.02827-12 · Cited by 181
Zheng, Z., Li, C., Liu, H., Zhang, Y., Zhong, X., & Yao, M. (2014) · Fuel · doi:10.1016/j.fuel.2014.10.053 · Cited by 177
Synthetic Biology and Metabolic Engineering Approaches To Produce Biofuels
Rabinovitch-Deere, C. A., Oliver, J. W. K., Rodriguez, G. M., & Atsumi, S. (2013) · Chemical Reviews · doi:10.1021/cr300361t · Cited by 177
Self-citationMicrobial biosynthesis of the anticoagulant precursor 4-hydroxycoumarin
Lin, Y., Shen, X., Yuan, Q., & Yan, Y. (2013) · Nature Communications · doi:10.1038/ncomms3603 · Cited by 175
De novo biosynthetic pathways: rational design of microbial chemical factories
Prather, K. L. J., & Martin, C. H. (2008) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2008.07.009 · Cited by 173
Principal component analysis of proteomics (PCAP) as a tool to direct metabolic engineering
Alonso-Gutiérrez, J., Kim, E., Batth, T. S., Cho, N. H., Hu, Q., Chan, L. J. G., Petzold, C. J., Hillson, N. J., Adams, P. D., Keasling, J. D., et al. (2014) · Metabolic Engineering · doi:10.1016/j.ymben.2014.11.011 · Cited by 174
Towards systems metabolic engineering of microorganisms for amino acid production
Park, J. H., & Lee, S. Y. (2008) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2008.08.007 · Cited by 171
Cofactor engineering for more efficient production of chemicals and biofuels
Wang, M., Chen, B., Fang, Y., & Tan, T. (2017) · Biotechnology Advances · doi:10.1016/j.biotechadv.2017.09.008 · Cited by 171
l -Malate Production by Metabolically Engineered Escherichia coli
Zhang, X., Wang, X., Shanmugam, K. T., & Ingram, L. O. (2010) · Applied and Environmental Microbiology · doi:10.1128/aem.01971-10 · Cited by 171
Metabolic engineering delivers next-generation biofuels
Keasling, J. D., & Chou, H. H. (2008) · Nature Biotechnology · doi:10.1038/nbt0308-298 · Cited by 170
Kumar, B. R., Saravanan, S., Rana, D. K., & Nagendran, A. (2016) · Energy Conversion and Management · doi:10.1016/j.enconman.2016.04.053 · Cited by 170
Separation and purification of biobutanol during bioconversion of biomass
Huang, H., Ramaswamy, S., & Liu, Y. (2014) · Separation and Purification Technology · doi:10.1016/j.seppur.2014.06.013 · Cited by 170
Engineering microbial consortia to enhance biomining and bioremediation
Brune, K. D., & Bayer, T. S. (2012) · Frontiers in Microbiology · doi:10.3389/fmicb.2012.00203 · Cited by 169
Engineering synergy in biotechnology
Nielsen, J., Fussenegger, M., Keasling, J. D., Lee, S. Y., Liao, J. C., Prather, K. L. J., & Palsson, B. Ø. (2014) · Nature Chemical Biology · doi:10.1038/nchembio.1519 · Cited by 167
Self-citationKondo, T., Tezuka, H., Ishii, J., Matsuda, F., Ogino, C., & Kondo, A. (2012) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2012.01.022 · Cited by 168
Autoignition of n-butanol at elevated pressure and low-to-intermediate temperature
Weber, B. W., Kumar, K., Zhang, Y., & Sung, C. (2011) · Combustion and Flame · doi:10.1016/j.combustflame.2011.02.005 · Cited by 165
Production of biofuels and chemicals from xylose using native and engineered yeast strains
Kwak, S., Jo, J.-H., Yun, E. J., Jin, Y., & Seo, J. (2018) · Biotechnology Advances · doi:10.1016/j.biotechadv.2018.12.003 · Cited by 165
Jang, Y., Park, J. M., Choi, S., Choi, Y. J., Seung, D. Y., Cho, J. H., & Lee, S. Y. (2011) · Biotechnology Advances · doi:10.1016/j.biotechadv.2011.08.015 · Cited by 166
Recent advances in microbial production of δ-aminolevulinic acid and vitamin B12
Kang, Z., Zhang, J., Zhou, J., Qi, Q., Du, G., & Chen, J. (2012) · Biotechnology Advances · doi:10.1016/j.biotechadv.2012.04.003 · Cited by 166
Fermentative production of butanol — the academic perspective
Dürre, P. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.04.010 · Cited by 163
Engineering of an Escherichia coli Strain for the Production of 3-Methyl-1-Butanol
Connor, M. R., & Liao, J. C. (2008) · Applied and Environmental Microbiology · doi:10.1128/aem.00468-08 · Cited by 163
Self-citationEngineering Escherichia coli for efficient production of 5-aminolevulinic acid from glucose
Kang, Z., Wang, Y., Gu, P., Wang, Q., & Qi, Q. (2011) · Metabolic Engineering · doi:10.1016/j.ymben.2011.05.003 · Cited by 163
Metadata
- Authors
- Atsumi, S., Hanai, T., & Liao, J. C.
- Affiliations
- University of California, Los Angeles (US)
- Corresponding author
- Liao, James C.
- Venue
- Nature
- Published
- 2008-01
- Volume
- 451
- Issue
- 7174
- Pages
- 86-89
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1978119727
- Cited by
- 1,945
- References
- 32
- Citations by year
- 2026: 50 · 2025: 55 · 2024: 66 · 2023: 72 · 2022: 113 · 2021: 111 · 2020: 121 · 2019: 86 · 2018: 98 · 2017: 103 · 2016: 131 · 2015: 137 · 2014: 148 · 2013: 153 · 2012: 162
- FWCI
- 46.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- U.S. Department of Energy
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Biochemical and biochemical processes
- Keywords
- isobutanol, n-butanol, 2-phenylethanol, Escherichia coli metabolic engineering, glucose fermentation, biofuels, gasoline substitute, octane number
- MeSH terms
- Butanols, Bioelectric Energy Sources, Escherichia coli, Fermentation, Genetic Engineering, Glucose
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
