The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels
Abstract
Global energy and environmental problems have stimulated increased efforts towards synthesizing biofuels from renewable resources. Compared to the traditional biofuel, ethanol, higher alcohols offer advantages as gasoline substitutes because of their higher energy density and lower hygroscopicity. In addition, branched-chain alcohols have higher octane numbers compared with their straight-chain counterparts. However, these alcohols cannot be synthesized economically using native organisms. Here we present a metabolic engineering approach using Escherichia coli to produce higher alcohols including isobutanol, 1-butanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from glucose, a renewable carbon source. This strategy uses the host's highly active amino acid biosynthetic pathway and diverts its 2-keto acid intermediates for alcohol synthesis. In particular, we have achieved high-yield, high-specificity production of isobutanol from glucose. The strategy enables the exploration of biofuels beyond those naturally accumulated to high quantities in microbial fermentation.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“The cyanobacterial cumulative titre of 1247 mg/L over 58 days equates to approximately 21.5 mg L−1 d−1 average daily productivity, compared to approximately 10,900 mg L−1 d−1 for E. coli—a several-hundred-fold difference reflecting light-dependent growth constraints rather than poor pathway design.”
From Cyanobacteria as a Photosynthetic Chassis for Metabolic Pathway Engineering with Heterologous Gene Expression (2026) · cites this paper as [175]
In the citing paper
“The longer chain alcohols possess higher energy content and can be stored and transported easier than ethanol (Atsumi et al. 2008).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Atsumi et al. 2008
In the citing paper
“The production of longer chain alcohols (C5–C–8) in E. coli bacteria has been achieved by the overproduction of 2-keto acids, intermediates of the amino acid biosynthesis. These intermediates were later converted to butanol derivatives by the heterologous expression of 2-keto acid decarboxylase and alcohol dehydrogenase (Atsumi et al. 2008; Zhang et al. 2008).”
From Renewable energy from Cyanobacteria: energy production optimization by metabolic pathway engineering (2011) · cites this paper as Atsumi et al. 2008
In the citing paper
“It is difficult to identify a microorganism suitable to produce C5 and other C4 substituted alcohols from sugar glucose while minimising the steps in the process and making it cost effective [141].”
From Investigation of Biofuel as a Potential Renewable Energy Source (2021) · cites this paper as [141]
Citations
Cites
Cox, B. M., Opheim, K. E., Teschemacher, H., & D, A. G. M. (1975) · Life Sciences · doi:10.1016/0024-3205(75)90272-6 · Cited by 1,708
Lutz, R. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.6.1203 · Cited by 1,694
Engineering Yeast Transcription Machinery for Improved Ethanol Tolerance and Production
Alper, H., Moxley, J., Nevoigt, E., Fink, G. R., & Stephanopoulos, G. (2006) · Science · doi:10.1126/science.1131969 · Cited by 823
Improving lycopene production in Escherichia coli by engineering metabolic control
Farmer, W. R., & Liao, J. C. (2000) · Nature Biotechnology · doi:10.1038/75398 · Cited by 525
Self-citationBalancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli
Pitera, D. J., Paddon, C. J., Newman, J. D., & Keasling, J. D. (2006) · Metabolic Engineering · doi:10.1016/j.ymben.2006.11.002 · Cited by 440
The Catabolism of Amino Acids to Long Chain and Complex Alcohols in Saccharomyces cerevisiae
Dickinson, J. R., Salgado, L. E. J., & Hewlins, M. J. E. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m211914200 · Cited by 375
Enteric Bacterial Catalysts for Fuel Ethanol Production
Ingram, L. O., Aldrich, H. C., Borges, A., Causey, T. B., Martı́nez, A., Martínez‐Morales, F., Saleh, A. Z., Underwood, S. A., Yomano, L. P., York, S. W., et al. (1999) · Biotechnology Progress · doi:10.1021/bp9901062 · Cited by 272
Metabolic engineering for drug discovery and development
Khosla, C., & Keasling, J. D. (2003) · Nature Reviews Drug Discovery · doi:10.1038/nrd1256 · Cited by 208
Vuralhan, Z., de Morais, M. A., Tai, S. L., Piper, M. D. W., & Pronk, J. T. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.8.4534-4541.2003 · Cited by 206
Nucleotide sequence of the yeast alcohol dehydrogenase II gene.
Russell, D. W., Smith, M. J., Williamson, V. M., & Young, E. T. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)32979-x · Cited by 205
Flagged first, then by citations
Cited by
Sorted by citations
Items 401–500 of 1,004
Biological Conversion of Amino Acids to Higher Alcohols
El-Dalatony, M. M., Saha, S., Govindwar, S. P., Abou-Shanab, R. A. I., & Jeon, B. (2019) · Trends in biotechnology · doi:10.1016/j.tibtech.2019.01.011 · Cited by 94
Policy Implications of Uncertainty in Modeled Life-Cycle Greenhouse Gas Emissions of Biofuels ⊥
Ichael, W. M., Riffin, G., & Cott, H. S. (2011) · Cited by 94
Metabolic engineering of Saccharomyces cerevisiae for production of butanol isomers
Generoso, W. C., Schadeweg, V., Oreb, M., & Boles, E. (2014) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2014.09.004 · Cited by 94
Zhuang, Q., Wang, Q., Liang, Q., & Qi, Q. (2014) · Metabolic Engineering · doi:10.1016/j.ymben.2014.05.004 · Cited by 93
Large-Scale Bi-Level Strain Design Approaches and Mixed-Integer Programming Solution Techniques
Kim, J., Reed, J. L., & Maravelias, C. T. (2011) · PLoS ONE · doi:10.1371/journal.pone.0024162 · Cited by 93
de Jong, B. W., Siewers, V., & Nielsen, J. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.11.021 · Cited by 93
Synthesis of n‐Propanol from CO 2 Electroreduction on Bicontinuous Cu 2 O/Cu Nanodomains
Zhang, R., Zhang, J., Wang, S., Tan, Z., Yang, Y., Song, Y., Li, M., Zhao, Y., Wang, H., Han, B., et al. (2024) · Angewandte Chemie International Edition · doi:10.1002/anie.202405733 · Cited by 95
Impact of bioethanol impurities on steam reforming for hydrogen production: A review
Sánchez, N., Ruíz, Y., Hacker, V., & Cobo, M. I. (2020) · International Journal of Hydrogen Energy · doi:10.1016/j.ijhydene.2020.02.159 · Cited by 92
Engineering modular ester fermentative pathways in Escherichia coli
Layton, D. S., & Trinh, C. T. (2014) · Metabolic Engineering · doi:10.1016/j.ymben.2014.09.006 · Cited by 89
A Novel Muconic Acid Biosynthesis Approach by Shunting Tryptophan Biosynthesis via Anthranilate
Sun, X., Lin, Y., Huang, Q., Yuan, Q., & Yan, Y. (2013) · Applied and Environmental Microbiology · doi:10.1128/aem.00859-13 · Cited by 92
Dynamic gene expression engineering as a tool in pathway engineering
Shen, X., Wang, J., Li, C., Yuan, Q., & Yan, Y. (2019) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2019.03.019 · Cited by 90
Synthesis at the Interface of Chemistry and Biology
Wu, X., & Schultz, P. G. (2009) · Journal of the American Chemical Society · doi:10.1021/ja9026067 · Cited by 90
Michener, J. K., Nielsen, J., & Smolke, C. D. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1212287109 · Cited by 91
Schiel‐Bengelsdorf, B., Montoya, J. Z., Linder, S., & Dürre, P. (2013) · Environmental Technology · doi:10.1080/09593330.2013.827746 · Cited by 91
Wiatrowski, M., Klein, B. C., Davis, R. W., Quiroz-Arita, C., Tan, E. C. D., Hunt, R. W., & Davis, R. E. (2022) · Biotechnology for Biofuels and Bioproducts · doi:10.1186/s13068-021-02098-3 · Cited by 90
Rate constants for hydrogen-abstraction by O˙H from n-butanol
Zhou, C., Simmie, J. M., & Curran, H. J. (2010) · Combustion and Flame · doi:10.1016/j.combustflame.2010.11.002 · Cited by 90
Utilizing an endogenous pathway for 1-butanol production in Saccharomyces cerevisiae
Si, T., Luo, Y., Xiao, H., & Zhao, H. (2014) · Metabolic Engineering · doi:10.1016/j.ymben.2014.01.002 · Cited by 90
The heat release analysis of bio-butanol/gasoline blends on a high speed SI (spark ignition) engine
Deng, B., Fu, J., Zhang, D., Yang, J., Feng, R., Liu, J., Li, K., & Liu, X. (2013) · Energy · doi:10.1016/j.energy.2013.07.055 · Cited by 90
Metabolic engineering of Zymomonas mobilis for anaerobic isobutanol production
Qiu, M., Shen, W., Yan, X., He, Q., Cai, D., Chen, S., Wei, H., Knoshaug, E. P., Zhang, M., Himmel, M. E., et al. (2020) · Biotechnology for Biofuels · doi:10.1186/s13068-020-1654-x · Cited by 90
Isobutanol production freed from biological limits using synthetic biochemistry
Sherkhanov, S., Korman, T. P., Chan, S., Faham, S., Liu, H., Sawaya, M. R., Hsu, W.-T., Vikram, E. P., Cheng, T., & Bowie, J. U. (2020) · Nature Communications · doi:10.1038/s41467-020-18124-1 · Cited by 93
Optimized gene expression from bacterial chromosome by high-throughput integration and screening
Saleski, T. E., Chung, M. T., Carruthers, D. N., Khasbaatar, A., Kurabayashi, K., & Lin, X. N. (2021) · Science Advances · doi:10.1126/sciadv.abe1767 · Cited by 90
Efficient way for detection of alcohols using hollow core photonic crystal fiber sensor
Habib, M. A., Anower, M. S., AlGhamdi, A. S., Faragallah, O. S., Eid, M. M. A., & Rashed, A. N. Z. (2021) · Optical Review · doi:10.1007/s10043-021-00672-6 · Cited by 90
Biological conversion of carbon dioxide to photosynthetic fuels and electrofuels
Li, H., & Liao, J. C. (2013) · Energy & Environmental Science · doi:10.1039/c3ee41847b · Cited by 92
Self-citationA Synthetic Metabolic Pathway for Production of the Platform Chemical Isobutyric Acid
Zhang, K., Woodruff, A. P., Xiong, M., Zhou, J., & Dhande, Y. K. (2011) · ChemSusChem · doi:10.1002/cssc.201100045 · Cited by 90
Synthetic Biology Guides Biofuel Production
Connor, M. R., & Atsumi, S. (2010) · BioMed Research International · doi:10.1155/2010/541698 · Cited by 91
Self-citationAb Initio Study of the Decomposition of 2,5-Dimethylfuran
Simmie, J. M., & Metcalfe, W. K. (2011) · The Journal of Physical Chemistry A · doi:10.1021/jp2039477 · Cited by 91
Rational engineering of diol dehydratase enables 1,4-butanediol biosynthesis from xylose
Wang, J., Jain, R., Shen, X., Sun, X., Cheng, M., Liao, J. C., Yuan, Q., & Yan, Y. (2017) · Metabolic Engineering · doi:10.1016/j.ymben.2017.02.003 · Cited by 90
Self-citationConverting Sugars to Biofuels: Ethanol and Beyond
Kang, A., & Lee, T. S. (2015) · Bioengineering · doi:10.3390/bioengineering2040184 · Cited by 90
Engineering of transcriptional regulators enhances microbial stress tolerance
Lin, Z., Zhang, Y., & Wang, J. (2013) · Biotechnology Advances · doi:10.1016/j.biotechadv.2013.02.010 · Cited by 87
Metabolic Engineering of Microorganisms for the Production of Higher Alcohols
Choi, Y. J., Lee, J., Jang, Y., & Lee, S. Y. (2014) · mBio · doi:10.1128/mbio.01524-14 · Cited by 89
Short-chain ketone production by engineered polyketide synthases in Streptomyces albus
Yuzawa, S., Mirsiaghi, M., Jocic, R., Fujii, T., Masson, F., Benites, V. T., Baidoo, E. E. K., Sundstrom, E. R., Tanjore, D., Pray, T. R., et al. (2018) · Nature Communications · doi:10.1038/s41467-018-07040-0 · Cited by 88
Formation Enthalpies and Bond Dissociation Energies of Alkylfurans. The Strongest C—X Bonds Known?
Simmie, J. M., & Curran, H. J. (2009) · The Journal of Physical Chemistry A · doi:10.1021/jp810315n · Cited by 88
Yang, J. E., Choi, Y. J., Lee, S. J., Kang, K. H., Lee, H., Oh, Y. H., Lee, S. H., Park, S. J., & Lee, S. Y. (2013) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-013-5285-z · Cited by 87
Engineering microbial chemical factories to produce renewable “biomonomers”
Adkins, J., Pugh, S., McKenna, R., & Nielsen, D. R. (2012) · Frontiers in Microbiology · doi:10.3389/fmicb.2012.00313 · Cited by 88
de Leeuw, K. D., Buisman, C. J. N., & Strik, D. P. B. T. B. (2019) · Environmental Science & Technology · doi:10.1021/acs.est.8b07256 · Cited by 86
Characteristics of Isopentanol as a Fuel for HCCI Engines
Yang, Y., Dec, J. E., Dronniou, N., & Simmons, B. A. (2010) · SAE international journal of fuels and lubricants · doi:10.4271/2010-01-2164 · Cited by 87
Park, S.-H., Kim, S., & Hahn, J. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-6081-0 · Cited by 87
Miao, R., Liu, X., Englund, E., Lindberg, P., & Lindblad, P. C. (2017) · Metabolic Engineering Communications · doi:10.1016/j.meteno.2017.07.003 · Cited by 88
Soma, Y., Fujiwara, Y., Nakagawa, T., Tsuruno, K., & Hanai, T. (2017) · Metabolic Engineering · doi:10.1016/j.ymben.2017.08.002 · Cited by 86
Self-citationThe fourth generation of biofuel
Moravvej, Z., Makarem, M. A., & Rahimpour, M. R. (2019) · Elsevier eBooks · doi:10.1016/b978-0-12-815162-4.00020-3 · Cited by 92
An experimental investigation on multi-cylinder RCCI engine fueled with 2-butanol/diesel
Pan, S., Li, X., Han, W., & Huang, Y. (2017) · Energy Conversion and Management · doi:10.1016/j.enconman.2017.10.047 · Cited by 86
Metabolic engineering of a synergistic pathway for n-butanol production in Saccharomyces cerevisiae
Shi, S., Si, T., Liu, Z., Zhang, H., Ang, E. L., & Zhao, H. (2016) · Scientific Reports · doi:10.1038/srep25675 · Cited by 85
Engineering Synechococcus elongatus PCC 7942 for Continuous Growth under Diurnal Conditions
McEwen, J. T., Machado, I. M. P., Connor, M. R., & Atsumi, S. (2012) · Applied and Environmental Microbiology · doi:10.1128/aem.03326-12 · Cited by 84
Self-citationZhu, C., Shen, T., Liu, D., Wu, J., Chen, Y., Wang, L., Guo, K., Ying, H., & Ouyang, P. (2015) · Green Chemistry · doi:10.1039/c5gc02414e · Cited by 86
Renata, H., Wang, Z. J., & Arnold, F. H. (2015) · Angewandte Chemie · doi:10.1002/ange.201409470 · Cited by 84
Establishment of BmoR-based biosensor to screen isobutanol overproducer
Yu, H., Wang, N., Huo, W., Zhang, Y., Zhang, W., Yang, Y., Chen, Z., & Huo, Y. (2019) · Microbial Cell Factories · doi:10.1186/s12934-019-1084-2 · Cited by 84
Antonyraj, A. P. M., & Muralitharan, G. (2014) · Bioresource Technology · doi:10.1016/j.biortech.2014.11.003 · Cited by 84
Energy coupling in Saccharomyces cerevisiae: selected opportunities for metabolic engineering
de Kok, S., Kozak, B. U., Pronk, J. T., & van Maris, A. J. A. (2012) · FEMS Yeast Research · doi:10.1111/j.1567-1364.2012.00799.x · Cited by 85
Engineering Biomass Conversion Processes: A Systems Perspective
Daoutidis, P., Marvin, W. A., Rangarajan, S., & Torres, A. I. (2012) · AIChE Journal · doi:10.1002/aic.13978 · Cited by 87
The role of alcohol biofuels in advanced combustion: An analysis
Gainey, B., & Lawler, B. J. (2020) · Fuel · doi:10.1016/j.fuel.2020.118915 · Cited by 83
Lang, K., Zierow, J., Bühler, K., & Schmid, A. (2014) · Microbial Cell Factories · doi:10.1186/1475-2859-13-2 · Cited by 83
Biofuel production by in vitro synthetic enzymatic pathway biotransformation
Zhang, Y. P., Sun, J., & Zhong, J. (2010) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2010.05.005 · Cited by 87
Bacterial synthesis of C3-C5 diols via extending amino acid catabolism
Wang, J., Li, C., Zou, Y., & Yan, Y. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2003032117 · Cited by 82
He, N., Zhu, X., Liu, F., Yu, R., Xue, Z., & Liu, X. (2022) · Chemical Engineering Journal · doi:10.1016/j.cej.2022.136543 · Cited by 82
Direct Extraction of Photosynthetic Electrons from Single Algal Cells by Nanoprobing System
Ryu, W., Bai, S. J., Park, J. S., Huang, Z., Moseley, J. L., Fabian, T., Fasching, R. J., Grossman, A. R., & Prinz, F. B. (2010) · Nano Letters · doi:10.1021/nl903141j · Cited by 82
Role of Escherichia coli in Biofuel Production
Koppolu, V., & Vasigala, V. K. (2016) · Microbiology Insights · doi:10.4137/mbi.s10878 · Cited by 84
Chen, T., Wang, J., Yang, R., Li, J., Lin, M., & Lin, Z. (2011) · PLoS ONE · doi:10.1371/journal.pone.0016228 · Cited by 81
Fisher, A. K., Freedman, B. G., Bevan, D. R., & Senger, R. S. (2014) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2014.08.010 · Cited by 81
Biobutanol Production from Crystalline Cellulose through Consolidated Bioprocessing
Xin, F., Dong, W., Zhang, W., Ma, J., & Jiang, M. (2018) · Trends in biotechnology · doi:10.1016/j.tibtech.2018.08.007 · Cited by 81
Elfasakhany, A. (2015) · Fuel · doi:10.1016/j.fuel.2015.09.059 · Cited by 80
Zhan, Y., Shi, J., Xiao, Y., Zhou, F., Wang, H., Xu, H., Li, Z., Yang, S., Cai, D., & Chen, S. (2022) · Metabolic Engineering · doi:10.1016/j.ymben.2022.01.007 · Cited by 80
Guo, B., Zhang, J., Zhang, W., Chen, F., & Liu, B. (2023) · Critical Reviews in Food Science and Nutrition · doi:10.1080/10408398.2023.2272769 · Cited by 88
An Expanded Synthetic Biology Toolkit for Gene Expression Control in Acetobacteraceae
Teh, M. Y., Ooi, K. H., Teo, S. X. D., Mansoor, M. E. B., Lim, W. Z. S., & Tan, M. H. (2019) · ACS Synthetic Biology · doi:10.1021/acssynbio.8b00168 · Cited by 79
New Octane Booster Molecules for Modern Gasoline Composition
Badia, J. H., Ramírez, E., Bringué, R., Cunill, F., & Delgado, J. (2021) · Energy & Fuels · doi:10.1021/acs.energyfuels.1c00912 · Cited by 79
Zhu, L., Dong, H., Zhang, Y., & Li, Y. (2011) · Metabolic Engineering · doi:10.1016/j.ymben.2011.01.009 · Cited by 79
Griffin, M. A., Spakowicz, D. J., Gianoulis, T. A., & Strobel, S. A. (2010) · Microbiology · doi:10.1099/mic.0.041327-0 · Cited by 79
Engineered biosynthesis of medium-chain esters in Escherichia coli
Tai, Y.-S., Xiong, M., & Zhang, K. (2014) · Metabolic Engineering · doi:10.1016/j.ymben.2014.10.004 · Cited by 79
Recent advances on bio-based isobutanol separation
Fu, C., Li, Z., Jia, C., Zhang, W., Zhang, Y., Yi, C., & Xie, S. (2020) · Energy Conversion and Management X · doi:10.1016/j.ecmx.2020.100059 · Cited by 78
Redox-switch regulatory mechanism of thiolase from Clostridium acetobutylicum
Kim, S., Jang, Y., Ha, S.-C., Ahn, J., Kim, E., Lim, J. H., Cho, C., Ryu, Y. S., Lee, S. K., Lee, S. Y., et al. (2015) · Nature Communications · doi:10.1038/ncomms9410 · Cited by 78
Reassessing Escherichia coli as a cell factory for biofuel production
Wang, C., Pfleger, B. F., & Kim, S.-W. (2017) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2017.02.010 · Cited by 78
Li, J., Zhang, W., Li, X., Ye, T., Gan, Y., Ai, Z., Hong, C., Xue, G., & Liu, Y. (2017) · Bioresource Technology · doi:10.1016/j.biortech.2017.09.186 · Cited by 78
Incorporation of unnatural amino acids for synthetic biology
Волощук, Н. І., & Montclare, J. K. (2009) · Molecular BioSystems · doi:10.1039/b909200p · Cited by 77
Metabolic engineering for sustainability and health
Kim, G. B., Choi, S. Y., Cho, I., Ahn, D., & Lee, S. Y. (2023) · Trends in biotechnology · doi:10.1016/j.tibtech.2022.12.014 · Cited by 77
Gas Fermentation for Commercial Biofuels Production
Min, F., Köpke, M., & Dennis, S. (2013) · InTech eBooks · doi:10.5772/52164 · Cited by 83
Biotechnological production of alpha-keto acids: Current status and perspectives
Song, Y., Li, J., Shin, H., Liu, L., Du, G., & Chen, J. (2016) · Bioresource Technology · doi:10.1016/j.biortech.2016.08.015 · Cited by 77
A molecular rheostat maintains ATP levels to drive a synthetic biochemistry system
Opgenorth, P. H., Korman, T. P., Iancu, L., & Bowie, J. U. (2017) · Nature Chemical Biology · doi:10.1038/nchembio.2418 · Cited by 78
Protein engineering approaches to chemical biotechnology
Chen, Z., & Zeng, A. (2016) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2016.07.007 · Cited by 76
Synthetic Metabolism: Engineering Biology at the Protein and Pathway Scales
Martin, C. H., Nielsen, D. R., Solomon, K., & Prather, K. L. J. (2009) · Chemistry & Biology · doi:10.1016/j.chembiol.2009.01.010 · Cited by 76
Al-Shorgani, N. K. N., Ali, E., Kalil, M. S., & Yusoff, W. M. W. (2011) · BioEnergy Research · doi:10.1007/s12155-011-9126-6 · Cited by 76
Walther, T., Topham, C. M., Irague, R., Auriol, C., Baylac, A., Cordier, H., Dressaire, C., Lozano-Huguet, L., Tarrat, N., Martineau, N., et al. (2017) · Nature Communications · doi:10.1038/ncomms15828 · Cited by 76
Igbokwe, V. C., Ezugworie, F. N., Onwosi, C. O., Aliyu, G. O., & Obi, C. J. (2021) · Journal of Environmental Management · doi:10.1016/j.jenvman.2021.114333 · Cited by 77
Enhancing the Co-utilization of Biomass-Derived Mixed Sugars by Yeasts
Gao, M., Ploessl, D. S., & Shao, Z. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.03264 · Cited by 79
Engineering Escherichia coli to convert acetic acid to free fatty acids
Xiao, Y., Ruan, Z., Liu, Z., Wu, S. G., Varman, A. M., Liu, Y., & Tang, Y. (2013) · Biochemical Engineering Journal · doi:10.1016/j.bej.2013.04.013 · Cited by 74
Kanno, M., Katayama, T., Tamaki, H., Mitani, Y., Meng, X., Hori, T., Narihiro, T., Morita, N., Hoshino, T., Yumoto, I., et al. (2013) · Applied and Environmental Microbiology · doi:10.1128/aem.02900-13 · Cited by 74
Wang, J., Mahajani, M., Jackson, S. L., Yang, Y., Chen, M., Ferreira, E. M., Lin, Y., & Yan, Y. (2017) · Metabolic Engineering · doi:10.1016/j.ymben.2017.09.011 · Cited by 73
Wieczorek, A. S., & Martin, V. J. J. (2010) · Microbial Cell Factories · doi:10.1186/1475-2859-9-69 · Cited by 73
A novel MVA-mediated pathway for isoprene production in engineered E. coli
Yang, J., Nie, Q., Liu, H., Xian, M., & Liu, H. (2016) · BMC Biotechnology · doi:10.1186/s12896-016-0236-2 · Cited by 73
Production of 2-methyl-1-butanol and 3-methyl-1-butanol in engineered Corynebacterium glutamicum
Vogt, M., Brüsseler, C., van Ooyen, J., Bott, M. H., & Marienhagen, J. (2016) · Metabolic Engineering · doi:10.1016/j.ymben.2016.10.007 · Cited by 72
Qu, G., Guo, J., Yang, D., & Sun, Z. (2018) · Green Chemistry · doi:10.1039/c7gc03046k · Cited by 73
Experimental studies on combustion and emissions of RCCI fueled with n-heptane/alcohols fuels
Qian, Y., Ouyang, L., Wang, X., Zhu, L., & Lü, X. (2015) · Fuel · doi:10.1016/j.fuel.2015.09.022 · Cited by 72
Fang, M., Wang, T., Zhang, C., Bai, J., Zheng, X., Zhao, X., Lou, C., & Xing, X. (2015) · Metabolic Engineering · doi:10.1016/j.ymben.2015.10.006 · Cited by 73
Biofuels: Biomolecular Engineering Fundamentals and Advances
Li, H., Cann, A. F., & Liao, J. C. (2010) · Annual Review of Chemical and Biomolecular Engineering · doi:10.1146/annurev-chembioeng-073009-100938 · Cited by 71
Self-citationUllah, M. W., Ul‐Islam, M., Khan, S., Shah, N., & Park, J. K. (2017) · Korean Journal of Chemical Engineering · doi:10.1007/s11814-017-0121-2 · Cited by 73
Siemerink, M. A. J., Kuit, W., López-Contreras, A. M., Eggink, G., John van der, O. O. S. T., & Kengen, S. W. M. (2011) · Applied and Environmental Microbiology · doi:10.1128/aem.01616-10 · Cited by 71
Gao, Q., Cao, X. D., Huang, Y., Yang, J., Chen, J., Wei, L., & Hua, Q. (2018) · ACS Synthetic Biology · doi:10.1021/acssynbio.7b00453 · Cited by 71
Zhang, Y., Cortez, J. D., Hammer, S. K., Carrasco‐López, C., Echauri, S. A. G., Wiggins, J. B., Wang, W., & Avalos, J. L. (2022) · Nature Communications · doi:10.1038/s41467-021-27852-x · Cited by 71
Chen, Z., Wu, Y., Huang, J., & Liu, D. (2015) · Bioresource Technology · doi:10.1016/j.biortech.2015.08.086 · Cited by 71
Synergy as design principle for metabolic engineering of 1-propanol production in Escherichia coli
Shen, C. R., & Liao, J. C. (2013) · Metabolic Engineering · doi:10.1016/j.ymben.2013.01.008 · Cited by 71
Self-citationThe antioxidant hydroxytyrosol: biotechnological production challenges and opportunities
Achmon, Y., & Fishman, A. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-6310-6 · Cited by 71
Industrial fermentation of renewable diesel fuels
Westfall, P. J., & Gardner, T. S. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.04.023 · Cited by 71
Metadata
- Authors
- Atsumi, S., Hanai, T., & Liao, J. C.
- Affiliations
- University of California, Los Angeles (US)
- Corresponding author
- Liao, James C.
- Venue
- Nature
- Published
- 2008-01
- Volume
- 451
- Issue
- 7174
- Pages
- 86-89
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W1978119727
- Cited by
- 1,945
- References
- 32
- Citations by year
- 2026: 50 · 2025: 55 · 2024: 66 · 2023: 72 · 2022: 113 · 2021: 111 · 2020: 121 · 2019: 86 · 2018: 98 · 2017: 103 · 2016: 131 · 2015: 137 · 2014: 148 · 2013: 153 · 2012: 162
- FWCI
- 46.30
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Funders
- U.S. Department of Energy
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Biochemical and biochemical processes
- Keywords
- isobutanol, n-butanol, 2-phenylethanol, Escherichia coli metabolic engineering, glucose fermentation, biofuels, gasoline substitute, octane number
- MeSH terms
- Butanols, Bioelectric Energy Sources, Escherichia coli, Fermentation, Genetic Engineering, Glucose
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
