Cited by 1,945Microbial Metabolic Engineering and BioproductionBiofuel production and bioconversionBiochemical and biochemical processes

The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.

Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels

Atsumi, Shota, Hanai, Taizo, Liao, James C.·2008·Nature

DOI 10.1038/nature06450

Open this paper in CoChat — free account

Abstract

Global energy and environmental problems have stimulated increased efforts towards synthesizing biofuels from renewable resources. Compared to the traditional biofuel, ethanol, higher alcohols offer advantages as gasoline substitutes because of their higher energy density and lower hygroscopicity. In addition, branched-chain alcohols have higher octane numbers compared with their straight-chain counterparts. However, these alcohols cannot be synthesized economically using native organisms. Here we present a metabolic engineering approach using Escherichia coli to produce higher alcohols including isobutanol, 1-butanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from glucose, a renewable carbon source. This strategy uses the host's highly active amino acid biosynthetic pathway and diverts its 2-keto acid intermediates for alcohol synthesis. In particular, we have achieved high-yield, high-specificity production of isobutanol from glucose. The strategy enables the exploration of biofuels beyond those naturally accumulated to high quantities in microbial fermentation.

Integrity signals

Registry

Crossref · 10.1038/nature06450

Cites

32 of 32 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Purification and properties
Cox, B. M., Opheim, K. E., Teschemacher, H., & D, A. G. M. (1975) · Life Sciences · doi:10.1016/0024-3205(75)90272-6 · Cited by 1,708
Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements
Lutz, R. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.6.1203 · Cited by 1,694
Engineering Yeast Transcription Machinery for Improved Ethanol Tolerance and Production
Alper, H., Moxley, J., Nevoigt, E., Fink, G. R., & Stephanopoulos, G. (2006) · Science · doi:10.1126/science.1131969 · Cited by 823
Improving lycopene production in Escherichia coli by engineering metabolic control
Farmer, W. R., & Liao, J. C. (2000) · Nature Biotechnology · doi:10.1038/75398 · Cited by 525
Self-citation
Balancing a heterologous mevalonate pathway for improved isoprenoid production in Escherichia coli
Pitera, D. J., Paddon, C. J., Newman, J. D., & Keasling, J. D. (2006) · Metabolic Engineering · doi:10.1016/j.ymben.2006.11.002 · Cited by 440
The Catabolism of Amino Acids to Long Chain and Complex Alcohols in Saccharomyces cerevisiae
Dickinson, J. R., Salgado, L. E. J., & Hewlins, M. J. E. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m211914200 · Cited by 375
Enteric Bacterial Catalysts for Fuel Ethanol Production
Ingram, L. O., Aldrich, H. C., Borges, A., Causey, T. B., Martı́nez, A., Martínez‐Morales, F., Saleh, A. Z., Underwood, S. A., Yomano, L. P., York, S. W., et al. (1999) · Biotechnology Progress · doi:10.1021/bp9901062 · Cited by 272
Metabolic engineering for drug discovery and development
Khosla, C., & Keasling, J. D. (2003) · Nature Reviews Drug Discovery · doi:10.1038/nrd1256 · Cited by 208
Identification and Characterization of Phenylpyruvate Decarboxylase Genes in Saccharomyces cerevisiae
Vuralhan, Z., de Morais, M. A., Tai, S. L., Piper, M. D. W., & Pronk, J. T. (2003) · Applied and Environmental Microbiology · doi:10.1128/aem.69.8.4534-4541.2003 · Cited by 206
Nucleotide sequence of the yeast alcohol dehydrogenase II gene.
Russell, D. W., Smith, M. J., Williamson, V. M., & Young, E. T. (1983) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)32979-x · Cited by 205

Flagged first, then by citations

Show all 32 references

Sorted by citations

Items 601–700 of 1,004

Reducing the allowable kinetic space by constructing ensemble of dynamic models with the same steady-state flux
Tan, Y., Rivera, J. G. L., Contador, C. A., Asenjo, J. A., & Liao, J. C. (2010) · Metabolic Engineering · doi:10.1016/j.ymben.2010.11.001 · Cited by 58
Self-citation
Dehydratase mediated 1-propanol production in metabolically engineered Escherichia coli
Jain, R., & Yan, Y. (2011) · Microbial Cell Factories · doi:10.1186/1475-2859-10-97 · Cited by 57
Engineering Escherichia coli to produce branched-chain fatty acids in high percentages
Bentley, G. J., Jiang, W. J., Guamán, L. P., Xiao, Y., & Zhang, F. (2016) · Metabolic Engineering · doi:10.1016/j.ymben.2016.07.003 · Cited by 57
Escherichia coli as a platform microbial host for systems metabolic engineering
Yang, D., Prabowo, C. P. S., Eun, H., Park, S. Y., Cho, I., Jiao, S., & Lee, S. Y. (2021) · Essays in Biochemistry · doi:10.1042/ebc20200172 · Cited by 56
Characterization of a thiamin diphosphate‐dependent phenylpyruvate decarboxylase from Saccharomyces cerevisiae
Kneen, M. M., Stan, R. C., Yep, A., Tyler, R. P., Saehuan, C., & McLeish, M. J. (2011) · FEBS Journal · doi:10.1111/j.1742-4658.2011.08103.x · Cited by 56
Design and development of synthetic microbial platform cells for bioenergy
Lee, S. J., Lee, S. J., & Lee, D.-W. (2013) · Frontiers in Microbiology · doi:10.3389/fmicb.2013.00092 · Cited by 57
Biosynthesis and genomic analysis of medium-chain hydrocarbon production by the endophytic fungal isolate Nigrograna mackinnonii E5202H
Shaw, J. J., Spakowicz, D. J., Dalal, R. S., Davis, J., Lehr, N. A., Dunican, B. F., Orellana, E. A., Narváez‐Trujillo, A., & Strobel, S. A. (2015) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-6206-5 · Cited by 56
In silico metabolic engineering of Bacillus subtilis for improved production of riboflavin, Egl-237, ( R , R )-2,3-butanediol and isobutanol
Hao, T., Han, B., Ma, H., Fu, J., Wang, H., Wang, Z., Tang, B., Chen, T., & Zhao, X. (2013) · Molecular BioSystems · doi:10.1039/c3mb25568a · Cited by 57
Branched-Chain Amino Acids
Yamamoto, K., Tsuchisaka, A., & Yukawa, H. (2016) · Advances in biochemical engineering, biotechnology · doi:10.1007/10_2016_28 · Cited by 56
Mini-review: In vitro Metabolic Engineering for Biomanufacturing of High-value Products
Guo, W., Sheng, J., & Feng, X. (2017) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2017.01.006 · Cited by 56
Genetic engineering of non-native hosts for 1-butanol production and its challenges: a review
Nawab, S., Wang, N., Ma, X., & Huo, Y. (2020) · Microbial Cell Factories · doi:10.1186/s12934-020-01337-w · Cited by 55
Microbial engineering for the production of isobutanol: current status and future directions
Lakshmi, N. M., Binod, P., Sindhu, R., Awasthi, M. K., & Pandey, A. (2021) · Bioengineered · doi:10.1080/21655979.2021.1978189 · Cited by 55
Metabolic Regulation and Coordination of the Metabolism in Bacteria in Response to a Variety of Growth Conditions
Shimizu, K. (2015) · Advances in biochemical engineering, biotechnology · doi:10.1007/10_2015_320 · Cited by 54
Global Metabolomic and Network analysis of Escherichia coli Responses to Exogenous Biofuels
Wang, J., Chen, L., Tian, X., Gao, L., Niu, X., Shi, M., & Zhang, W. (2013) · Journal of Proteome Research · doi:10.1021/pr400640u · Cited by 56
Photosynthetic terpene hydrocarbon production for fuels and chemicals
Wang, X., Ort, D. R., & Yuan, J. S. (2015) · Plant Biotechnology Journal · doi:10.1111/pbi.12343 · Cited by 55
Effects of direct injection timing and premixed ratio on combustion and emissions characteristics of RCCI (Reactivity Controlled Compression Ignition) with N-heptane/gasoline-like fuels
Zhu, L., Qian, Y., Wang, X., & Lü, X. (2015) · Energy · doi:10.1016/j.energy.2015.09.069 · Cited by 55
Recent advances and challenges of the use of cyanobacteria towards the production of biofuels
Singh, V., Chaudhary, D. K., Mani, I., & Dhar, P. K. (2016) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2016.01.099 · Cited by 56
Biocatalysis – Key enabling tools from biocatalytic one-step and multi-step reactions to biocatalytic total synthesis
Wohlgemuth, R. (2020) · New Biotechnology · doi:10.1016/j.nbt.2020.08.006 · Cited by 55
Nature‐Inspired Construction of MOF@COF Nanozyme with Active Sites in Tailored Microenvironment and Pseudopodia‐Like Surface for Enhanced Bacterial Inhibition
Zhang, L., Liu, Z., Deng, Q., Sang, Y., Dong, K., Ren, J., & Qu, X. (2020) · Angewandte Chemie · doi:10.1002/ange.202012487 · Cited by 54
Development of Isopentanol Reaction Mechanism Reproducing Autoignition Character at High and Low Temperatures
Tsujimura, T., Pitz, W. J., Gillespie, F., Curran, H. J., Weber, B. W., Zhang, Y., & Sung, C. (2012) · Energy & Fuels · doi:10.1021/ef300879k · Cited by 54
Decreased production of higher alcohols by Saccharomyces cerevisiae for Chinese rice wine fermentation by deletion of Bat aminotransferases
Zhang, C., Qi, Y., Ma, H.-X., Li, W., Dai, L., & Xiao, D. (2015) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-015-1583-z · Cited by 54
Establishment of a novel gene expression method, BICES (biomass-inducible chromosome-based expression system), and its application to the production of 2,3-butanediol and acetoin
Nakashima, N., Akita, H., & Hoshino, T. (2014) · Metabolic Engineering · doi:10.1016/j.ymben.2014.07.011 · Cited by 54
Experimental and Kinetic Modeling Study of 2-Methylfuran Pyrolysis at Low and Atmospheric Pressures
Cheng, Z., He, S., Xing, L., Wei, L., Li, W., Li, T., Yan, B., Ma, W., & Chen, G. (2016) · Energy & Fuels · doi:10.1021/acs.energyfuels.6b02399 · Cited by 54
Engineered microbial biofuel production and recovery under supercritical carbon dioxide
Boock, J. T., Freedman, A. J., Tompsett, G. A., Muse, S. K., Allen, A. J., Jackson, L. A., Dominguez, B. C., Timko, M. T., Prather, K. L. J., & Thompson, J. R. (2019) · Nature Communications · doi:10.1038/s41467-019-08486-6 · Cited by 54
Whole Cell Actinobacteria as Biocatalysts
Anteneh, Y. S., & Franco, C. M. M. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.00077 · Cited by 57
A Pseudomonas putida efflux pump acts on short-chain alcohols
Basler, G., Thompson, M. G., Tullman‐Ercek, D., & Keasling, J. D. (2018) · Biotechnology for Biofuels · doi:10.1186/s13068-018-1133-9 · Cited by 53
Consolidated Bioprocessing: Synthetic Biology Routes to Fuels and Fine Chemicals
Banner, A., Toogood, H. S., & Scrutton, N. S. (2021) · Microorganisms · doi:10.3390/microorganisms9051079 · Cited by 57
Metabolic engineering of Corynebacterium glutamicum for producing branched chain amino acids
Yu, S., Zheng, B., Chen, Z., & Huo, Y. (2021) · Microbial Cell Factories · doi:10.1186/s12934-021-01721-0 · Cited by 53
Bioconversion of Hemicellulose from Sugarcane Biomass Into Sustainable Products
Canilha, L., Rodrigues, R. D. C. L. B., Fernandes, F. A., Kumar, A., Milessi, T. S., Felipe, M. D. G. D. A., & da, S. S. (2013) · InTech eBooks · doi:10.5772/53832 · Cited by 54
1-Butanol ignition delay times at low temperatures: An application of the constrained-reaction-volume strategy
Zhu, Y., Davidson, D. F., & Hanson, R. K. (2013) · Combustion and Flame · doi:10.1016/j.combustflame.2013.06.028 · Cited by 53
Biological Production of Muconic Acid via a Prokaryotic 2,3‐Dihydroxybenzoic Acid Decarboxylase
Sun, X., Lin, Y., Yuan, Q., & Yan, Y. (2014) · ChemSusChem · doi:10.1002/cssc.201402092 · Cited by 53
The application of powerful promoters to enhance gene expression in industrial microorganisms
Zhou, S., Du, G., Kang, Z., Li, J., Chen, J., Li, H., & Zhou, J. (2017) · World Journal of Microbiology and Biotechnology · doi:10.1007/s11274-016-2184-3 · Cited by 53
Extremely Fast NADH-Regeneration Using Phosphonic Acid as Hydride Source and Iridium-pyridine-2-sulfonamidate Catalysts
Tensi, L., & Macchioni, A. (2020) · ACS Catalysis · doi:10.1021/acscatal.0c02261 · Cited by 53
Light-energy conversion in engineered microorganisms
Johnson, E. T., & Schmidt‐Dannert, C. (2008) · Trends in biotechnology · doi:10.1016/j.tibtech.2008.09.002 · Cited by 53
Metabolic engineering of clostridia for the production of chemicals
Cho, C., Jang, Y., Moon, H. G., Lee, J., & Lee, S. Y. (2014) · Biofuels Bioproducts and Biorefining · doi:10.1002/bbb.1531 · Cited by 52
Sooting behaviors of n-butanol and n-dodecane blends
Ghiassi, H., Tóth, P., & Lighty, J. S. (2013) · Combustion and Flame · doi:10.1016/j.combustflame.2013.10.011 · Cited by 52
Engineering synthetic recursive pathways to generate non-natural small molecules
Felnagle, E. A., Chaubey, A., Noey, E. L., Houk, K. N., & Liao, J. C. (2012) · Nature Chemical Biology · doi:10.1038/nchembio.959 · Cited by 52
Self-citation
The predictive capability of an automatically generated combustion chemistry mechanism: Chemical structures of premixed iso-butanol flames
Hansen, N., Merchant, S. S., Harper, M. R., & Green, W. H. (2013) · Combustion and Flame · doi:10.1016/j.combustflame.2013.05.013 · Cited by 52
Physiological adaptations of Saccharomyces cerevisiae evolved for improved butanol tolerance
Ghiaci, P., Norbeck, J., & Larsson, C. (2013) · Biotechnology for Biofuels · doi:10.1186/1754-6834-6-101 · Cited by 52
Eliminating the isoleucine biosynthetic pathway to reduce competitive carbon outflow during isobutanol production by Saccharomyces cerevisiae
Ida, K., Ishii, J., Matsuda, F., Kondo, T., & Kondo, A. (2015) · Microbial Cell Factories · doi:10.1186/s12934-015-0240-6 · Cited by 52
Syntrophic co-culture amplification of production phenotype for high-throughput screening of microbial strain libraries
Saleski, T. E., Kerner, A., Chung, M. T., Jackman, C. M., Khasbaatar, A., Kurabayashi, K., & Lin, X. N. (2019) · Metabolic Engineering · doi:10.1016/j.ymben.2019.04.007 · Cited by 53
Systems Metabolic Engineering of Escherichia coli
Choi, K. R., Shin, J. H., Cho, J. S., Yang, D., & Lee, S. Y. (2016) · EcoSal Plus · doi:10.1128/ecosalplus.esp-0010-2015 · Cited by 53
Metabolic Engineering of Escherichia coli for Production of 2-phenylethanol from Renewable Glucose
Kang, Z., Zhang, C., Du, G., & Chen, J. (2013) · Applied Biochemistry and Biotechnology · doi:10.1007/s12010-013-0659-3 · Cited by 51
Biomass Production from Electricity Using Ammonia as an Electron Carrier in a Reverse Microbial Fuel Cell
Khunjar, W., Sahin, A., West, A. C., Chandran, K., & Banta, S. A. (2012) · PLoS ONE · doi:10.1371/journal.pone.0044846 · Cited by 51
Alternative Acetate Production Pathways in Chlamydomonas reinhardtii during Dark Anoxia and the Dominant Role of Chloroplasts in Fermentative Acetate Production
Yang, W., Catalanotti, C., D’Adamo, S., Wittkopp, T. M., Ingram-Smith, C. J., Mackinder, L. C. M., Miller, T. E., Heuberger, A. L., Peers, G., Smith, K. S., et al. (2014) · The Plant Cell · doi:10.1105/tpc.114.129965 · Cited by 51
Engineering strategy of yeast metabolism for higher alcohol production
Matsuda, F., Furusawa, C., Kondo, T., Ishii, J., Shimizu, H., & Kondo, A. (2011) · Microbial Cell Factories · doi:10.1186/1475-2859-10-70 · Cited by 52
Biotechnological and bioinformatics approaches for augmentation of biohydrogen production: A review
Kumar, G. R., & Chowdhary, N. (2015) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2015.12.022 · Cited by 51
Study and reengineering of the binding sites and allosteric regulation of biosynthetic threonine deaminase by isoleucine and valine in Escherichia coli
Chen, L., Chen, Z., Zheng, P., Sun, J., & Zeng, A. (2012) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-012-4176-z · Cited by 51
Quantitative analysis of volatile metabolites released in vitro by bacteria of the genus Stenotrophomonas for identification of breath biomarkers of respiratory infection in cystic fibrosis.
Shestivská, V., Dryahina, K., Nunvář, J., Sovová, K., Elhottová, D., Nemec, A., Smith, D. C., & Španěl, P. (2015) · Journal of Breath Research · doi:10.1088/1752-7155/9/2/027104 · Cited by 50
Enhancement of (R)-1,3-butanediol production by engineered Escherichia coli using a bioreactor system with strict regulation of overall oxygen transfer coefficient and pH
Kataoka, N., Vangnai, A. S., Ueda, H., Tajima, T., Nakashimada, Y., & Kato, J. (2014) · Bioscience Biotechnology and Biochemistry · doi:10.1080/09168451.2014.891933 · Cited by 52
Engineering cyanobacteria for fuels and chemicals production
Zhou, J., & Li, Y. (2010) · Protein & Cell · doi:10.1007/s13238-010-0043-9 · Cited by 53
Isobutanol production from cellobiose in Escherichia coli
Desai, S. H., Rabinovitch-Deere, C. A., Tashiro, Y., & Atsumi, S. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-013-5504-7 · Cited by 50
Self-citation
Molecular Breeding of Advanced Microorganisms for Biofuel Production
Sakuragi, H., Kuroda, K., & Ueda, M. (2011) · BioMed Research International · doi:10.1155/2011/416931 · Cited by 50
Structural synthetic biotechnology: from molecular structure to predictable design for industrial strain development
Chen, Z., Wilmanns, M., & Zeng, A. (2010) · Trends in biotechnology · doi:10.1016/j.tibtech.2010.07.004 · Cited by 50
Optimal metabolic route search based on atom mappings
Latendresse, M., Krummenacker, M., & Karp, P. D. (2014) · Bioinformatics · doi:10.1093/bioinformatics/btu150 · Cited by 50
Elucidation of the roles of adhE1 and adhE2 in the primary metabolism of Clostridium acetobutylicum by combining in-frame gene deletion and a quantitative system-scale approach
Yoo, M., Croux, C., Meynial‐Salles, I., & Soucaille, P. (2016) · Biotechnology for Biofuels · doi:10.1186/s13068-016-0507-0 · Cited by 50
Metabolically engineered Saccharomyces cerevisiae for enhanced isoamyl alcohol production
Yuan, J., Chen, X., Mishra, P., & Ching, C. (2016) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-016-7970-1 · Cited by 50
Isobutanol and 2-ketoisovalerate production by Klebsiella pneumoniae via a native pathway
Gu, J., Zhou, J., Zhang, Z., Kim, C. H., Jiang, B., Shi, J., & Hao, J. (2017) · Metabolic Engineering · doi:10.1016/j.ymben.2017.07.003 · Cited by 50
Metabolic engineering of Escherichia coli for the de novo stereospecific biosynthesis of 1,2-propanediol through lactic acid
Niu, W., Kramer, L., Mueller, J., Liu, K., & Guo, J. (2018) · Metabolic Engineering Communications · doi:10.1016/j.mec.2018.e00082 · Cited by 50
A roadmap towards integrated catalytic systems of the future
Burgener, S., Luo, S., McLean, R. P., Miller, T. E., & Erb, T. J. (2020) · Nature Catalysis · doi:10.1038/s41929-020-0429-x · Cited by 50
An updated review on advancement in fermentative production strategies for biobutanol using Clostridium spp.
Vamsi Krishna, K., Bharathi, N., George Shiju, S., Alagesan Paari, K., & Malaviya, A. (2022) · Environmental Science and Pollution Research · doi:10.1007/s11356-022-20637-9 · Cited by 51
Rapid dissection of a complex phenotype through genomic-scale mapping of fitness altering genes
Warnecke, T., Lynch, M., Karimpour‐Fard, A., Lipscomb, M. L., Handke, P., Mills, T., Ramey, C. J., Hoang, T., & Gill, R. T. (2010) · Metabolic Engineering · doi:10.1016/j.ymben.2009.12.002 · Cited by 49
Protein design in metabolic engineering and synthetic biology
Pleiss, J. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.03.004 · Cited by 49
Protein engineering for metabolic engineering: Current and next‐generation tools
Marcheschi, R. J., Gronenberg, L. S., & Liao, J. C. (2013) · Biotechnology Journal · doi:10.1002/biot.201200371 · Cited by 49
Self-citation
Microbial 1‐butanol production: Identification of non‐native production routes and in silico engineering interventions
Ranganathan, S., & Maranas, C. D. (2010) · Biotechnology Journal · doi:10.1002/biot.201000171 · Cited by 49
An ancient Chinese wisdom for metabolic engineering: Yin-Yang
Wu, S. G., He, L., Wang, Q., & Tang, Y. (2015) · Microbial Cell Factories · doi:10.1186/s12934-015-0219-3 · Cited by 52
Improvement of isopropanol tolerance of Escherichia coli using adaptive laboratory evolution and omics technologies
Horinouchi, T., Sakai, A., Kotani, H., Tanabe, K., & Furusawa, C. (2017) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2017.06.408 · Cited by 50
Toward industrial production of isoprenoids in Escherichia coli: Lessons learned from CRISPR‐Cas9 based optimization of a chromosomally integrated mevalonate pathway
Alonso-Gutiérrez, J., Koma, D., Hu, Q., Yang, Y., Chan, L. J. G., Petzold, C. J., Adams, P. D., Vickers, C. E., Nielsen, L. K., Keasling, J. D., et al. (2017) · Biotechnology and Bioengineering · doi:10.1002/bit.26530 · Cited by 49
Systems biology based metabolic engineering for non-natural chemicals
Biz, A., Proulx, S., Xu, Z.-Q. J., Siddartha, K., Indrayanti, A. M., & Mahadevan, R. (2019) · Biotechnology Advances · doi:10.1016/j.biotechadv.2019.04.001 · Cited by 49
Microbial Engineering for Production of N‐Functionalized Amino Acids and Amines
Mindt, M., Walter, T., Kugler, P., & Wendisch, V. F. (2020) · Biotechnology Journal · doi:10.1002/biot.201900451 · Cited by 49
Multi-feedstock lignocellulosic biorefineries based on biological processes: An overview
García‐Ochoa, F., Vergara, P., Wojtusik, M., Gutiérrez, S., Santos, V. E., Ladero, M., & Villar, J. C. (2021) · Industrial Crops and Products · doi:10.1016/j.indcrop.2021.114062 · Cited by 49
One-step continuous process for the production of 1-butanol and 1-hexanol by catalytic conversion of bio-ethanol at its sub-/supercritical state
Ghaziaskar, H. S., & Xu, C. C. (2013) · RSC Advances · doi:10.1039/c3ra00134b · Cited by 49
Reverse β-oxidation pathways for efficient chemical production
Tarasava, K., Lee, S. H., Chen, J., Köpke, M., Jewett, M. C., & González, R. (2022) · Journal of Industrial Microbiology & Biotechnology · doi:10.1093/jimb/kuac003 · Cited by 48
Advances in metabolic engineering of cyanobacteria for production of biofuels
Mund, N. K., Liu, Y., & Chen, S. (2022) · Fuel · doi:10.1016/j.fuel.2022.124117 · Cited by 48
Conversion of Biomass into Bioplastics and Their Potential Environmental Impacts
Pei, L., Schmidt, M. R., & Wei, W. (2011) · InTech eBooks · doi:10.5772/18042 · Cited by 47
Systematic engineering of microorganisms to improve alcohol tolerance
Jia, K., Zhang, Y., & Li, Y. (2010) · Engineering in Life Sciences · doi:10.1002/elsc.201000076 · Cited by 48
Biobutanol from cheese whey
Becerra, M., Cerdán, M. E., & González-Siso, M.-I. (2015) · Microbial Cell Factories · doi:10.1186/s12934-015-0200-1 · Cited by 48
Cellulosic Butanol Production from Agricultural Biomass and Residues: Recent Advances in Technology
Qureshi, N., Liu, S., & Ezeji, T. C. (2012) · Springer eBooks · doi:10.1007/978-1-4614-3348-4_15 · Cited by 48
Engineering the leucine biosynthetic pathway for isoamyl alcohol overproduction in Saccharomyces cerevisiae
Yuan, J., Mishra, P., & Ching, C. B. (2016) · Journal of Industrial Microbiology & Biotechnology · doi:10.1007/s10295-016-1855-2 · Cited by 48
Unravelling water effects on solid acid catalysts: Case study of TiO 2 /SiO 2 as a catalyst for the dehydration of isobutanol
Buniazet, Z., Couble, J., Bianchi, D., Rivallan, M., Cabiac, A., Maury, S., & Loridant, S. (2017) · Journal of Catalysis · doi:10.1016/j.jcat.2016.12.007 · Cited by 48
Protein engineering of oxidoreductases utilizing nicotinamide-based coenzymes, with applications in synthetic biology
You, C., Huang, R., Wei, X., Zhu, Z., & Zhang, Y. ‐. P. (2017) · Synthetic and Systems Biotechnology · doi:10.1016/j.synbio.2017.09.002 · Cited by 48
One‐Pot Production of Natural 2‐Phenylethanol from L‐Phenylalanine via Cascade Biotransformations
Lukito, B. R., Wu, S., Saw, H. J. J., & Li, Z. (2018) · ChemCatChem · doi:10.1002/cctc.201801613 · Cited by 48
A Feasibility Study of Cellulosic Isobutanol Production—Process Simulation and Economic Analysis
Roussos, A., Misailidis, N., Koulouris, A., Zimbardi, F., & Petrides, D. P. (2019) · Processes · doi:10.3390/pr7100667 · Cited by 48
Evaluation of the potential feedstock for biojet fuel production: Focus in the Brazilian context
Escalante, E. S. R., Ramos, L. S., Coronado, C. J. R., & de Carvalho, J. A. (2021) · Renewable and Sustainable Energy Reviews · doi:10.1016/j.rser.2021.111716 · Cited by 53
Ethanol photocatalysis on rutile TiO 2 (110): the role of defects and water
Walenta, C. A., Kollmannsberger, S. L., Kiermaier, J., Winbauer, A., Tschurl, M., & Heiz, U. (2015) · Physical Chemistry Chemical Physics · doi:10.1039/c5cp03550c · Cited by 47
Catalyst activity comparison of alcohols over zeolites
Ramasamy, K. K., & Wang, Y. (2013) · Journal of Energy Chemistry · doi:10.1016/s2095-4956(13)60008-x · Cited by 47
Synthesis and physical properties of new estolide esters
Cermak, S. C., Bredsguard, J. W., John, B. L., McCalvin, J. S., Thompson, T. D., Isbell, K. N., Feken, K. A., Isbell, T. A., & Murray, R. E. (2013) · Industrial Crops and Products · doi:10.1016/j.indcrop.2013.02.006 · Cited by 47
Genome Engineering of the 2,3-Butanediol Biosynthetic Pathway for Tight Regulation in Cyanobacteria
Nozzi, N. E., & Atsumi, S. (2015) · ACS Synthetic Biology · doi:10.1021/acssynbio.5b00057 · Cited by 47
Self-citation
Selective upgrading of ethanol with methanol in water for the production of improved biofuel—isobutanol
Liu, Q., Xu, G., Wang, X., & Mu, X. (2016) · Green Chemistry · doi:10.1039/c5gc02963e · Cited by 47
High-throughput enzyme screening platform for the IPP-bypass mevalonate pathway for isopentenol production
Kang, A., Meadows, C. W., Canu, N., Keasling, J. D., & Lee, T. S. (2017) · Metabolic Engineering · doi:10.1016/j.ymben.2017.03.010 · Cited by 47
d-1,2,4-Butanetriol production from renewable biomass with optimization of synthetic pathway in engineered Escherichia coli
Wang, X., Xu, N., Hu, S., Yang, J., Gao, Q., Xu, S., Chen, K., & Ouyang, P. (2017) · Bioresource Technology · doi:10.1016/j.biortech.2017.11.062 · Cited by 47
Sugar- and Starch-Based Biorefineries
Höfer, R. (2015) · Elsevier eBooks · doi:10.1016/b978-0-444-63453-5.00005-7 · Cited by 47
A Comprehensive Functional Characterization of Escherichia coli Lipid Genes
Jeucken, A., Molenaar, M. R., van de Lest, C. H. A., Jansen, J. W. A., Helms, J. B., & Brouwers, J. F. H. M. (2019) · Cell Reports · doi:10.1016/j.celrep.2019.04.018 · Cited by 47
Engineering transcription factor BmoR for screening butanol overproducers
Yu, H., Chen, Z., Wang, N., Yu, S., Yan, Y., & Huo, Y. (2019) · Metabolic Engineering · doi:10.1016/j.ymben.2019.08.015 · Cited by 45
DEVELOPMENT OF MICROORGANISMS FOR CELLULOSE-BIOFUEL CONSOLIDATED BIOPROCESSINGS: METABOLIC ENGINEERS' TRICKS
Mazzoli, R. (2012) · Computational and Structural Biotechnology Journal · doi:10.5936/csbj.201210007 · Cited by 47
2-Butanol and Butanone Production in Saccharomyces cerevisiae through Combination of a B12 Dependent Dehydratase and a Secondary Alcohol Dehydrogenase Using a TEV-Based Expression System
Ghiaci, P., Norbeck, J., & Larsson, C. (2014) · PLoS ONE · doi:10.1371/journal.pone.0102774 · Cited by 46
EnzymeMap: curation, validation and data-driven prediction of enzymatic reactions
Heid, E., Probst, D., Green, W. H., & Madsen, G. K. H. (2023) · Chemical Science · doi:10.1039/d3sc02048g · Cited by 52
Biochemical, genetic, and metabolic engineering strategies to enhance coproduction of 1-propanol and ethanol in engineered Escherichia coli
Srirangan, K., Liu, X., Westbrook, A. W., Akawi, L., Pyne, M. E., Moo‐Young, M., & Chou, C.-H. P. (2014) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-6093-9 · Cited by 46
Bioenergy and Biofuel Production from Wastes/Residues of Emerging Biofuel Industries
Khanal, S. K., Rasmussen, M. L., Shrestha, P., Leeuwen, H. J. V., Visvanathan, C., & Liu, H. (2008) · Water Environment Research · doi:10.2175/106143008x328752 · Cited by 46
Metabolic pathway engineering: Perspectives and applications
Dasgupta, A., Chowdhury, N., & De, R. K. (2020) · Computer Methods and Programs in Biomedicine · doi:10.1016/j.cmpb.2020.105436 · Cited by 47

Authors: Atsumi, S., Hanai, T., & Liao, J. C.
Affiliations: University of California, Los Angeles (US)
Corresponding author: Liao, James C.
ORCID: Atsumi, Shota
Hanai, Taizo
Liao, James C.
Venue: Nature
Publisher: Springer Science and Business Media LLC
Published: 2008-01
Volume: 451
Issue: 7174
Pages: 86-89
ISSN: 0028-0836 (ISSN-L)
1476-4687
DOI: 10.1038/nature06450
OpenAlex: W1978119727
Cited by: 1,945
References: 32
Citations by year: 2026: 50 · 2025: 55 · 2024: 66 · 2023: 72 · 2022: 113 · 2021: 111 · 2020: 121 · 2019: 86 · 2018: 98 · 2017: 103 · 2016: 131 · 2015: 137 · 2014: 148 · 2013: 153 · 2012: 162
FWCI: 46.30
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature (publishedVersion)
PubMed (publishedVersion)
RePEc: Research Papers in Economics (submittedVersion)
Funders: U.S. Department of Energy
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Biochemical and biochemical processes
Keywords: isobutanol, n-butanol, 2-phenylethanol, Escherichia coli metabolic engineering, glucose fermentation, biofuels, gasoline substitute, octane number
MeSH terms: Butanols, Bioelectric Energy Sources, Escherichia coli, Fermentation, Genetic Engineering, Glucose

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Non-fermentative pathways for synthesis of branched-chain higher alcohols as biofuels

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Citations

Cites

Cited by

Related

Metadata

Record

About these checks