The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.
Cross-neutralization of influenza A viruses mediated by a single antibody loop
Open accessAbstract
Immune recognition of protein antigens relies on the combined interaction of multiple antibody loops, which provide a fairly large footprint and constrain the size and shape of protein surfaces that can be targeted. Single protein loops can mediate extremely high-affinity binding, but it is unclear whether such a mechanism is available to antibodies. Here we report the isolation and characterization of an antibody called C05, which neutralizes strains from multiple subtypes of influenza A virus, including H1, H2 and H3. X-ray and electron microscopy structures show that C05 recognizes conserved elements of the receptor-binding site on the haemagglutinin surface glycoprotein. Recognition of the haemagglutinin receptor-binding site is dominated by a single heavy-chain complementarity-determining region 3 loop, with minor contacts from heavy-chain complementarity-determining region 1, and is sufficient to achieve nanomolar binding with a minimal footprint. Thus, binding predominantly with a single loop can allow antibodies to target small, conserved functional sites on otherwise hypervariable antigens.
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Metadata
- Authors
- Ekiert, D. C., Kashyap, A. K., Steel, J., Rubrum, A., Bhabha, G., Khayat, R., Lee, J. H., Dillon, M. A., O’Neil, R. E., Faynboym, A. M., Horowitz, M., Horowitz, L., Ward, A. B., Palese, P., Webby, R., Lerner, R. A., Bhatt, R. R., & Wilson, I. A.
- Venue
- Nature
- Published
- 2012-09
- Volume
- 489
- Issue
- 7417
- Pages
- 526-532
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2077219861
- Cited by
- 481
- References
- 8
- FWCI
- 24.77
- Type
- journal article
- Language
- en
- Open access
- green
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- Ekiert, D. C., Kashyap, A. K., Steel, J., Rubrum, A., Bhabha, G., Khayat, R., Lee, J. H., Dillon, M. A., O’Neil, R. E., Faynboym, A. M., Horowitz, M., Horowitz, L., Ward, A. B., Palese, P., Webby, R., Lerner, R. A., Bhatt, R. R., & Wilson, I. A. (2012). Cross-neutralization of influenza A viruses mediated by a single antibody loop. Nature. https://doi.org/10.1038/nature11414
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
