Student or educator? Sign up with your school email and get 6 months of Pro free.
CoChat
Sign Up
Cited by 1,951

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Nanometre-scale thermometry in a living cell

Open access
Kucsko, G., Maurer, P. C., Yao, N. Y., Kubo, M., Noh, H. J., Lo, P. K., Park, H., Lukin, M. D.·2013·Nature
DOI 10.1038/nature12373
Open this paper in CoChat — free account

Abstract

Sensitive probing of temperature variations on nanometre scales is an outstanding challenge in many areas of modern science and technology. In particular, a thermometer capable of subdegree temperature resolution over a large range of temperatures as well as integration within a living system could provide a powerful new tool in many areas of biological, physical and chemical research. Possibilities range from the temperature-induced control of gene expression and tumour metabolism to the cell-selective treatment of disease and the study of heat dissipation in integrated circuits. By combining local light-induced heat sources with sensitive nanoscale thermometry, it may also be possible to engineer biological processes at the subcellular level. Here we demonstrate a new approach to nanoscale thermometry that uses coherent manipulation of the electronic spin associated with nitrogen–vacancy colour centres in diamond. Our technique makes it possible to detect temperature variations as small as 1.8 mK (a sensitivity of 9 mK Hz−1/2) in an ultrapure bulk diamond sample. Using nitrogen–vacancy centres in diamond nanocrystals (nanodiamonds), we directly measure the local thermal environment on length scales as short as 200 nanometres. Finally, by introducing both nanodiamonds and gold nanoparticles into a single human embryonic fibroblast, we demonstrate temperature-gradient control and mapping at the subcellular level, enabling unique potential applications in life sciences.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1038/nature12373

Cites

1 of 1 references verified0 citation errors · 0 not checkable

Statistics

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 1 references

Research works printed but not linked: 29

  1. Yue, Y. & Wang, X. Nanoscale thermal probing. Nano Rev. http://dx.doi.org/10.3402/nano.v3i0.11586 (2012) · DOI
  2. Lucchetta, E., Lee, J., Fu, L., Patel, N. & Ismagilov, R. Dynamics of Drosophila embryonic patterning network perturbed in space and time using microfluidics. Nature 434, 1134–1138 (2005) · DOI · Paper page
  3. Lauschke, V. M., Tsiairis, C. D., François, P. & Aulehla, A. Scaling of embryonic patterning based on phase-gradient encoding. Nature 493, 101–105 (2012) · DOI
  4. Kamei, Y. et al. Infrared laser-mediated gene induction in targeted single cells in vivo. Nature Methods 6, 79–81 (2009) · DOI · Paper page
  5. Vreugdenburg, T., Willis, C., Mundy, L. & Hiller, J. A systematic review of elastography, electrical impedance scanning, and digital infrared thermography for breast cancer screening and diagnosis. Breast Cancer Res. Treat. 137, 665–676 (2013) · DOI
  6. Schroeder, A. et al. Treating metastatic cancer with nanotechnology. Nature Rev. Cancer 12, 39–50 (2011) · DOI · Paper page
  7. O’Neal, D. P., Hirsch, L. R., Halas, N. J., Payne, J. D. & West, J. L. Photo-thermal tumor ablation in mice using near infrared-absorbing nanoparticles. Cancer Lett. 209, 171–176 (2004) · DOI · Paper page
  8. Majumdar, A. Scanning thermal microscopy. Annu. Rev. Mater. Sci. 29, 505–585 (1999) · DOI · Paper page
  9. Kim, S. H. et al. Micro-Raman thermometry for measuring the temperature distribution inside the microchannel of a polymerase chain reaction chip. J. Micromech. Microeng. 16, 526 (2006) · DOI
  10. Yang, J., Yang, H. & Lin, L. Quantum dot nano thermometers reveal heterogeneous local thermogenesis in living cells. ACS Nano 5, 5067–5071 (2011) · DOI · Paper page
  11. Vetrone, F. et al. Temperature sensing using fluorescent nanothermometers. ACS Nano 4, 3254–3258 (2010) · DOI · Paper page
  12. Okabe, K. et al. Intracellular temperature mapping with a fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy. Nature Commun. 3, 705 (2012) · DOI · Paper page
  13. Donner, J., Thompson, S., Kreuzer, M., Baffou, G. & Quidant, R. Mapping intracellular temperature using green fluorescent protein. Nano Lett. 12, 2107–2111 (2012) · DOI · Paper page
  14. Shalek, A. K. et al. Vertical silicon nanowires as a universal platform for delivering biomolecules into living cells. Proc. Natl Acad. Sci. USA 107, 1870–1875 (2010) · DOI · Paper page
  15. Acosta, V. et al. Temperature dependence of the nitrogen-vacancy magnetic resonance in diamond. Phys. Rev. Lett. 104, 070801 (2010) · DOI · Paper page
  16. Toyli, D. et al. Measurement and control of single nitrogen-vacancy center spins above 600 K. Phys. Rev. X 2, 031001 (2012)
  17. Chen, X.-D. et al. Temperature dependent energy level shifts of nitrogen-vacancy centers in diamond. Appl. Phys. Lett. 99, 161903–161903 (2011) · DOI · Paper page
  18. Taylor, J. et al. High-sensitivity diamond magnetometer with nanoscale resolution. Nature Phys. 4, 810–816 (2008) · DOI · Paper page
  19. Jin, C., Li, Z., Williams, R., Lee, K. & Park, I. Localized temperature and chemical reaction control in nanoscale space by nanowire array. Nano Lett. 11, 4818–4825 (2011) · doi:10.1021/nl2026585
  20. Maze, J. et al. Nanoscale magnetic sensing with an individual electronic spin in diamond. Nature 455, 644–647 (2008) · DOI · Paper page
  21. Balasubramanian, G. et al. Nanoscale imaging magnetometry with diamond spins under ambient conditions. Nature 455, 648–651 (2008) · DOI · Paper page
  22. Hodges, J. et al. Time keeping with electron spin states in diamond. Phys. Rev. A 87, 032118 (2013) · DOI
  23. Balasubramanian, G. et al. Ultralong spin coherence time in isotopically engineered diamond. Nature Mater. 8, 383–387 (2009) · DOI · Paper page
  24. Dolde, F. et al. Electric-field sensing using single diamond spins. Nature Phys. 7, 459–463 (2011) · DOI · Paper page
  25. Maurer, P. et al. Far-field optical imaging and manipulation of individual spins with nanoscale resolution. Nature Phys. 6, 912–918 (2010) · DOI · Paper page
  26. Xu, G. et al. Identification of proteins sensitive to thermal stress in human neuroblastoma and glioma cell lines. PLoS ONE 7, e49021 (2012) · DOI
  27. Helmchen, F. & Denk, W. Deep tissue two-photon microscopy. Nature Methods 2, 932–940 (2005) · DOI · Paper page
  28. Wee, T.-L. et al. Two-photon excited fluorescence of nitrogen-vacancy centers in proton-irradiated type Ib diamond. J. Phys. Chem. A 111, 9379–9386 (2007) · DOI
  29. Tsoli, M. et al. Activation of thermogenesis in brown adipose tissue and dysregulated lipid metabolism associated with cancer cachexia in mice. Cancer Res. 72, 4372–4382 (2012) · DOI · Paper page

Cited by

See all 1,951

Metadata

Authors
Kucsko, G., Maurer, P. C., Yao, N. Y., Kubo, M., Noh, H. J., Lo, P. K., Park, H., & Lukin, M. D.
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2013-07-31
Volume
500
Issue
7460
Pages
54-58
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
DOI
10.1038/nature12373
OpenAlex
W2159974629
Cited by
1,951
References
1
FWCI
50.92
Type
journal article
Language
en
Open access
bronze
Licence
www.springer.com/tdm (tdm)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

How to cite

Cite CoChat for the checks on this page, and the paper for its own findings.

This page
CoChat. (2026). Citation check: Nanometre-scale thermometry in a living cell. https://app.cochat.ai/papers/10.1038/nature12373
The paper
Kucsko, G., Maurer, P. C., Yao, N. Y., Kubo, M., Noh, H. J., Lo, P. K., Park, H., & Lukin, M. D. (2013). Nanometre-scale thermometry in a living cell. Nature. https://doi.org/10.1038/nature12373

Data sources

Bibliographic data: Semantic Scholar, OpenAlex, Crossref.

Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.