The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
Visualization of a short-range Wnt gradient in the intestinal stem-cell niche
Abstract
Mammalian Wnt proteins are believed to act as short-range signals, yet have not been previously visualized in vivo. Self-renewal, proliferation and differentiation are coordinated along a putative Wnt gradient in the intestinal crypt. Wnt3 is produced specifically by Paneth cells. Here we have generated an epitope-tagged, functional Wnt3 knock-in allele. Wnt3 covers basolateral membranes of neighbouring stem cells. In intestinal organoids, Wnt3-transfer involves direct contact between Paneth cells and stem cells. Plasma membrane localization requires surface expression of Frizzled receptors, which in turn is regulated by the transmembrane E3 ligases Rnf43/Znrf3 and their antagonists Lgr4-5/R-spondin. By manipulating Wnt3 secretion and by arresting stem-cell proliferation, we demonstrate that Wnt3 mainly travels away from its source in a cell-bound manner through cell division, and not through diffusion. We conclude that stem-cell membranes constitute a reservoir for Wnt proteins, while Frizzled receptor turnover and 'plasma membrane dilution' through cell division shape the epithelial Wnt3 gradient.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“Because it was recently reported that Wnt3 transfer requires direct cell contact and has only a very limited range (Farin et al., 2016), the most likely cellular source of Wnts that influence Wnt signaling upon aging might thus be mostly Paneth cells and, in part, mesenchyme.”
From Canonical Wnt Signaling Ameliorates Aging of Intestinal Stem Cells (2017) · cites this paper as Farin et al., 2016
In the citing paper
“Because it was recently reported that Wnt3 transfer requires direct cell contact and has only a very limited range (Farin et al., 2016)”
From Canonical Wnt Signaling Ameliorates Aging of Intestinal Stem Cells (2017) · cites this paper as Farin et al., 2016
Citations
Cites
Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche
Sato, T., Vries, R. G., Snippert, H. J., van de Wetering, M., Barker, N., Stange, D. E., van Es, J. H., Abo, A., Kujala, P., Peters, P. J., et al. (2009) · Nature · doi:10.1038/nature07935 · Cited by 7,204
Self-citationIdentification of stem cells in small intestine and colon by marker gene Lgr5
Barker, N., van Es, J. H., Kuipers, J., Kujala, P., van den Born, M., Cozijnsen, M., Haegebarth, A., Korving, J., Begthel, H., Peters, P. J., et al. (2007) · Nature · doi:10.1038/nature06196 · Cited by 5,687
Self-citationPaneth cells constitute the niche for Lgr5 stem cells in intestinal crypts
Sato, T., van Es, J. H., Snippert, H. J., Stange, D. E., Vries, R. G., van den Born, M., Barker, N., Shroyer, N. F., van de Wetering, M., & Clevers, H. (2011) · Nature · doi:10.1038/nature09637 · Cited by 2,510
Self-citationSchwank, G., Koo, B.-K., Sasselli, V., Dekkers, J. F., Heo, I., Demircan, T., Sasaki, N., Boymans, S., Cuppen, E., van der Ent, C. K., et al. (2013) · Cell Stem Cell · doi:10.1016/j.stem.2013.11.002 · Cited by 1,424
Self-citationSmall molecule–mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer
Chen, B., Dodge, M. E., Tang, W., Lü, J., Ma, Z., Fan, C.-W., Wei, S., Hao, W., Kilgore, J. A., Williams, N. S., et al. (2009) · Nature Chemical Biology · doi:10.1038/nchembio.137 · Cited by 1,402
The intestinal crypt, a prototype stem cell compartment
Clevers, H. (2013) · Cell · doi:10.1016/j.cell.2013.07.004 · Cited by 1,223
Self-citationTissue‐specific and inducible Cre‐mediated recombination in the gut epithelium
Marjou, F. E., Janssen, K., Chang, B. H., Li, M., Hindie, V., Chan, L., Louvard, D., Chambon, P., METZGER, D., & Robine, S. (2004) · genesis · doi:10.1002/gene.20042 · Cited by 1,087
Tumour suppressor RNF43 is a stem-cell E3 ligase that induces endocytosis of Wnt receptors
Koo, B.-K., Spit, M., Jordens, I., Low, T. Y., Stange, D. E., van de Wetering, M., van Es, J. H., Mohammed, S., Heck, A. J. R., Maurice, M. M., et al. (2012) · Nature · doi:10.1038/nature11308 · Cited by 980
Self-citationZNRF3 promotes Wnt receptor turnover in an R-spondin-sensitive manner
Hao, H.-X., Xie, Y., Zhang, Y., Charlat, O., Oster, E., Avello, M., Lei, H., Mickanin, C., Liu, D., Ruffner, H., et al. (2012) · Nature · doi:10.1038/nature11019 · Cited by 962
Structural Basis of Wnt Recognition by Frizzled
Janda, C. Y., Waghray, D., Levin, A. M., Thomas, C., & García, K. C. (2012) · Science · doi:10.1126/science.1222879 · Cited by 901
Flagged first, then by citations
Cited by
Sorted by citations
Items 301–400 of 525
Phosphorylation of PPDPF via IL6‐JAK2 activates the Wnt/β‐catenin pathway in colorectal cancer
Zi, Y., Liu, L.-Y. D., Gao, J., Xu, X., Guan, Y., Rong, Z., Cao, Z., Li, M., Zeng, Z., Fan, Q., et al. (2023) · EMBO Reports · doi:10.15252/embr.202255060 · Cited by 15
Cheng, X., Shen, H., Zhang, W., Chen, B., Xu, S., & Wu, L. (2024) · Journal of Hazardous Materials · doi:10.1016/j.jhazmat.2024.135734 · Cited by 16
Matsuda, M., & Sokol, S. Y. (2021) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/bs.ctdb.2021.04.001 · Cited by 16
Unraveling the Control of Cell Cycle Periods during Intestinal Stem Cell Differentiation
Ballweg, R., Lee, S., Han, X., Maini, P. K., Byrne, H. M., Hong, C. I., & Zhang, T. (2018) · Biophysical Journal · doi:10.1016/j.bpj.2018.10.025 · Cited by 16
Sievers, C. K., Leystra, A. A., Clipson, L. J., Dove, W. F., & Halberg, R. B. (2016) · Cancer Prevention Research · doi:10.1158/1940-6207.capr-16-0096 · Cited by 15
Mechano-Chemical Coupling in Hydra Regeneration and Patterning
Wang, R., Bialas, A. L., Goel, T., & Collins, E. S. (2023) · Integrative and Comparative Biology · doi:10.1093/icb/icad070 · Cited by 15
Sticking Around: Short-Range Activity of Wnt Ligands
Boutros, M., & Niehrs, C. (2016) · Developmental Cell · doi:10.1016/j.devcel.2016.02.018 · Cited by 15
Extracellular WNTs: Trafficking, Exosomes, and Ligand-Receptor Interaction.
Gross, J. C. (2021) · Handbook of Experimental Pharmacology · doi:10.1007/164_2021_531 · Cited by 12
The evolution of a new cell type was associated with competition for a signaling ligand
Ettensohn, C. A., & Adomako‐Ankomah, A. (2019) · PLoS Biology · doi:10.1371/journal.pbio.3000460 · Cited by 15
The logistics of Wnt production and delivery
Alvarez‐Rodrigo, I., Willnow, D., & Vincent, J. (2023) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/bs.ctdb.2023.01.006 · Cited by 12
Bigaeva, E., Bomers, J. J. M., Biel, C., Mutsaers, H. A. M., de Graaf, I. A. M., Boersema, M., & Olinga, P. (2019) · Toxicology in Vitro · doi:10.1016/j.tiv.2019.05.024 · Cited by 15
Regulation of stem and progenitor cells in the adrenal cortex
Finco, I., Mohan, D. R., Hammer, G. D., & Lerário, A. M. (2019) · Current Opinion in Endocrine and Metabolic Research · doi:10.1016/j.coemr.2019.07.009 · Cited by 14
Clonal analysis of stem cells in differentiation and disease
Colom, B., & Jones, P. H. (2016) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2016.07.002 · Cited by 13
Trentesaux, C., Striedinger, K., Pomerantz, J. H., & Klein, O. D. (2020) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2020.01.004 · Cited by 15
Modelling adult stem cells and their niche in health and disease with epithelial organoids
Szabó, L., Seubert, A. C., & Kretzschmar, K. (2022) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2022.09.006 · Cited by 14
The Colonic Crypt: Cellular Dynamics and Signaling Pathways in Homeostasis and Cancer
Nguyen, A. L., Lausten, M. A., & Boman, B. M. (2025) · Cells · doi:10.3390/cells14181428 · Cited by 13
Sending signals – The microbiota’s contribution to intestinal epithelial homeostasis
Goyal, S., Tsang, D. K. L., Maisonneuve, C., & Girardin, S. E. (2020) · Microbes and Infection · doi:10.1016/j.micinf.2020.10.009 · Cited by 15
Li, Y., Wang, X., Zhao, G., Deng, L., & Yin, X. (2023) · Stem Cells and Development · doi:10.1089/scd.2023.0186 · Cited by 14
Intestinal Paneth cell differentiation relies on asymmetric regulation of Wnt signaling by Daam1/2
Colozza, G., Lee, H., Merenda, A., Wu, S.-H. S., Català-Bordes, A., Radaszkiewicz, T. W., Jordens, I., Lee, J., Bamford, A.-D., Farnhammer, F., et al. (2023) · Science Advances · doi:10.1126/sciadv.adh9673 · Cited by 15
Self-citationLu, G. U. O., Yan, R. Y., Zheng, D., Li, H., Li, G. L., & Wu, S. H. (2023) · Experimental Gerontology · doi:10.1016/j.exger.2023.112351 · Cited by 13
Yang, Y., Deng, G., Qiao, L., Yuan, H., Yu, X., Xu, L., Lu, S., Jiang, W. B., & Yu, X. (2022) · Journal of Molecular Cell Biology · doi:10.1093/jmcb/mjac038 · Cited by 13
Bao, L., Cui, X., Bai, R., & Chen, C. (2022) · Nano Research · doi:10.1007/s12274-022-5150-4 · Cited by 12
Using 3D Organoid Cultures to Model Intestinal Physiology and Colorectal Cancer
Short, S. P., Costacurta, P. W., & Williams, C. S. (2017) · Current Colorectal Cancer Reports · doi:10.1007/s11888-017-0363-8 · Cited by 14
New insights in ubiquitin-dependent Wnt receptor regulation in tumorigenesis
Tsukiyama, T. (2024) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-024-00855-w · Cited by 11
Cao, H., Yue, L., Shao, J., Kong, F., Liu, S., Huai, H., He, Z., Mao, Z., Yang, Y., Tan, Y., et al. (2024) · Stem Cell Research & Therapy · doi:10.1186/s13287-024-04115-2 · Cited by 15
Positional BMP signaling orchestrates villus length in the small intestine
Wang, X., Li, S., Liu, Y., Kuang, X., Chen, J., Yu, P., Zhao, L., Zhang, Z., Huang, M., Liu, L., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-60643-2 · Cited by 13
Pluripotency state regulates cytoneme selectivity and self-organization of embryonic stem cells
Junyent, S., Reeves, J., Gentleman, E., & Habib, S. J. (2021) · The Journal of Cell Biology · doi:10.1083/jcb.202005095 · Cited by 12
Balance between the cell viability and death in 3D
Debruyne, A. C., Okkelman, I. A., & Dmitriev, R. I. (2022) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2022.09.005 · Cited by 16
Role of Wnt signaling in the maintenance and regeneration of the intestinal epithelium
Gasnier, M., Lim, H. Y. G., & Barker, N. (2023) · Current topics in developmental biology/Current Topics in Developmental Biology · doi:10.1016/bs.ctdb.2023.01.001 · Cited by 14
Environmental Impact on Intestinal Stem Cell Functions in Mucosal Homeostasis and Tumorigenesis
Augenlicht, L. H. (2016) · Journal of Cellular Biochemistry · doi:10.1002/jcb.25719 · Cited by 12
Chhabra, S., Liu, L., Goh, R., & Warmflash, A. (2018) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/440164 · Cited by 16
A 3D co-culture intestinal organoid system for exploring glucose metabolism
Nie, J., Liao, W., Zhang, Z., Zhang, M., Wen, Y., Çapanoğlu, E., Sarker, M. M. R., Zhu, R., & Zhao, C. (2022) · Current Research in Food Science · doi:10.1016/j.crfs.2022.11.021 · Cited by 11
Säisä-Borreill, S., Davidson, G., Kleiber, T., Thevenot, A., Martin, E., Mondot, S., Blottière, H. M., Helleux, A., Mengus, G., Plateroti, M., et al. (2023) · Cell Death and Differentiation · doi:10.1038/s41418-022-01109-6 · Cited by 11
M1 and M2 Macrophages Differentially Regulate Colonic Crypt Renewal
Raveenthiraraj, S., Awanis, G., Chieppa, M., O’Connell, A. E., & Sobolewski, A. (2023) · Inflammatory Bowel Diseases · doi:10.1093/ibd/izad270 · Cited by 11
Gall, L., Duckworth, C. A., Jardí, F., Lammens, L., Parker, A., Bianco, A., Kimko, H. H. C., Pritchard, D. M., & Pin, C. (2023) · eLife · doi:10.7554/elife.85478 · Cited by 12
Xie, D., Chen, B., Xue, Y., Sun, Z., Ning, B., Zhang, Z., Chi, J., Su, M., & Song, Y. (2024) · Science China Materials · doi:10.1007/s40843-024-2933-8 · Cited by 11
Yu, L.-E., Yang, W., & Liang, Y. (2024) · Biomedicines · doi:10.3390/biomedicines12122771 · Cited by 13
Diffusion and function of Wnt ligands
Stewart, R. A., Ramakrishnan, A., & Cadigan, K. M. (2019) · PLoS Genetics · doi:10.1371/journal.pgen.1008154 · Cited by 11
Philippi, M., Richter, C. P., Kappen, M., Watrinet, I., Miao, Y., Runge, M., Jorde, L., Korneev, S., Holtmannspötter, M., Kurre, R., et al. (2022) · Small · doi:10.1002/smll.202203723 · Cited by 11
Direct visualization of the Wntless-induced redistribution of WNT1 in developing chick embryos
Galli, L. M., Santana, F., Apollon, C., Szabo, L. A., Ngo, K. J., & Burrus, L. W. (2018) · Developmental Biology · doi:10.1016/j.ydbio.2018.04.025 · Cited by 13
Hmgcr promotes a long-range signal to attract Drosophila germ cells independently of Hedgehog
Kenwrick, K., Mukherjee, A., & Renault, A. D. (2019) · Journal of Cell Science · doi:10.1242/jcs.232637 · Cited by 11
Morphogen gradients are regulated by porous media characteristics of the developing tissue
Stark, J., Harish, R., Sbalzarini, I., & Brand, M. (2024) · bioRxiv · doi:10.1242/dev.204312 · Cited by 9
Cross-species comparison reveals therapeutic vulnerabilities halting glioblastoma progression
Foerster, L. C., Kaya, O., Wüst, V., Danciu, D.-P., Akcay, V., Bekavac, M., Ziegler, K. C., Stinchcombe, N., Tang, A., Kleber, S., et al. (2025) · Nature Communications · doi:10.1038/s41467-025-62528-w · Cited by 11
Tissue and cellular spatiotemporal dynamics in colon aging
Daly, A. C., Cambuli, F., Äijö, T., Lötstedt, B., Marjanovic, N. D., Fernandez, S., Kuksenko, O., Smith-Erb, M., Domovic, D., Wittenberghe, N. V., et al. (2025) · Nature Biotechnology · doi:10.1038/s41587-025-02830-6 · Cited by 11
Childress, K. O., Cencer, C. S., Tyska, M. J., & Lacy, D. B. (2023) · mBio · doi:10.1128/mbio.01857-23 · Cited by 10
Raineri, F., Bourgoin‐Voillard, S., Cossutta, M., Habert, D., Ponzo, M., Houppe, C., Vallée, B., Boniotto, M., Chalabi‐Dchar, M., Bouvet, P., et al. (2021) · Cancers · doi:10.3390/cancers13122986 · Cited by 10
Taking a Step Back: Insights into the Mechanisms Regulating Gut Epithelial Dedifferentiation
Ouladan, S., & Gregorieff, A. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22137043 · Cited by 9
Microbial Genes, Enzymes, and Metabolites: To Improve Rhizosphere and Plant Health Management
Fazeli‐Nasab, B., & Rahmani, A. F. (2021) · Springer eBooks · doi:10.1007/978-981-16-2922-8_19 · Cited by 11
Shifting the Focus of Signaling Abnormalities in Colon Cancer
Brown, M. A., & Ried, T. (2022) · Cancers · doi:10.3390/cancers14030784 · Cited by 11
Single-Molecule Imaging of Wnt3A Protein Diffusion on Living Cell Membranes
Lippert, A. H., Janeczek, A. A., Fürstenberg, A., Ponjavic, A., Moerner, W. E., Nusse, R., Helms, J. A., Evans, N. D., & Lee, S. F. (2017) · Biophysical Journal · doi:10.1016/j.bpj.2017.08.060 · Cited by 10
Dickkopf-1 blocks 17β-estradiol-enhanced object memory consolidation in ovariectomized female mice
Taxier, L. R., Philippi, S. M., Fortress, A. M., & Frick, K. M. (2019) · Hormones and Behavior · doi:10.1016/j.yhbeh.2019.06.009 · Cited by 10
Murrow, L. M., Weber, R. J., Caruso, J. A., McGinnis, C. S., Phong, K. T., Gascard, P. D., Borowsky, A. D., Desai, T. A., Thomson, M., Tlsty, T. D., et al. (2018) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/430611 · Cited by 11
Tu, J.-J., Zang, Y., Shi, Y. S., & Teng, X. (2024) · Cells · doi:10.3390/cells13211784 · Cited by 10
Matsumoto, K., Akieda, Y., Haraoka, Y., Hirono, N., Sasaki, H., & Ishitani, T. (2024) · Nature Communications · doi:10.1038/s41467-024-55108-x · Cited by 9
Long-range organization of intestinal 2D-crypts using exogenous Wnt3a micropatterning
Larrañaga, E., Marín-Riera, M., Abad‐Lázaro, A., Bartolomé-Català, D., Otero-Tarrazón, A., Fernández‐Majada, V., Batlle, E., Sharpe, J. A., Ojosnegros, S., Comelles, J., et al. (2025) · Nature Communications · doi:10.1038/s41467-024-55651-7 · Cited by 9
Boswell, C. W., Hoppe, C., Sherrard, A., Miao, L., Kojima, M. L., Martino, P. A., Zhao, N., Stasevich, T. J., Nicoli, S., & Giráldez, A. J. (2025) · Nature Communications · doi:10.1038/s41467-025-61003-w · Cited by 9
Roles of intestinal stem cells in inflammatory bowel disease pathogenesis
Zhang, M.-J., Chan, S.-X., Jia, Z.-G., Lv, C., Chen, J., & Hong, S. (2025) · World Journal of Stem Cells · doi:10.4252/wjsc.v17.i8.107639 · Cited by 10
Li, X., Shi, J., Zhao, W., Huang, X., Cui, L., Liu, L., Jin, X., Li, D., Zhang, X., & Du, M. (2022) · Reproduction · doi:10.1530/rep-21-0282 · Cited by 9
Transfer of polarity information via diffusion of Wnt ligands in C. elegans embryos
Recouvreux, P., Pai, P., Dunsing, V., Torro, R., Ludányi, M., Mélénec, P., Boughzala, M., Bertrand, V., & Lenne, P. (2024) · Current Biology · doi:10.1016/j.cub.2024.03.030 · Cited by 9
Wang, L., Long, J., Chen, H., Sun, S., Lv, K., Li, Q., & Wang, X. (2020) · International Journal of Cancer · doi:10.1002/ijc.33458 · Cited by 9
Jones, W. D., Guadiana, S. M., & Grove, E. A. (2019) · The Journal of Comparative Neurology · doi:10.1002/cne.24643 · Cited by 9
Spatially defined microenvironment for engineering organoids
Zhang, Y., Qi, F., Chen, P., Liu, B., & Li, Y. (2024) · Biophysics Reviews · doi:10.1063/5.0198848 · Cited by 9
Winsor, N. J., Tsang, D. K. L., Ranger, A., Singh, O., Goyal, S., Philpott, D. J., & Girardin, S. E. (2025) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2417149122 · Cited by 9
Telocytes deliver essential Wnts directly to murine intestinal stem cells via synapse-like contacts
Greicius, G., Mittermeier, L., Liang, R., Sigmundsson, K., Chan, Y. K., Liao, P.-J., Ludwig, A., & Virshup, D. M. (2025) · Developmental Cell · doi:10.1016/j.devcel.2025.06.040 · Cited by 8
Cambra, H. M., Tallapragada, N. P., Mannam, P., Breault, D. T., & Klein, A. M. (2022) · Current Protocols · doi:10.1002/cpz1.330 · Cited by 8
The Relationship between LRP6 and Wnt/β-Catenin Pathway in Colorectal and Esophageal Cancer
Shishido, A., Miyo, M., Oishi, K., Nishiyama, N., Wu, M., Yamamoto, H., Kouda, S., Wu, X., Shibata, S., Yokoyama, Y., et al. (2023) · Life · doi:10.3390/life13030615 · Cited by 11
Biased precursor ingression underlies the center-to-pole pattern of male sex determination in mouse
Bunce, C., Barske, L. A., Zhang, G. X., & Capel, B. (2023) · Development · doi:10.1242/dev.201060 · Cited by 8
Melamed, D., Choi, A., Reilein, A. R., Tavaré, S., & Kalderon, D. (2023) · PLoS Genetics · doi:10.1371/journal.pgen.1010965 · Cited by 7
Won, J.-H., & Cho, K.-O. (2020) · Cell Death and Differentiation · doi:10.1038/s41418-020-00632-8 · Cited by 8
Building bridges between fields: bringing together development and homeostasis
Weterings, S. D. C., van Oostrom, M. J., & Sonnen, K. F. (2021) · Development · doi:10.1242/dev.193268 · Cited by 10
Roads to the Fountain of Youth? Rejuvenating Intestinal Stem Cells
Larrick, J. W., & Mendelsohn, A. R. (2019) · Rejuvenation Research · doi:10.1089/rej.2019.2251 · Cited by 9
An Overview on the Pharmaceutical Applications of Nanocellulose
Kamel, R., & ElKasabgy, N. A. (2023) · Composites science and technology · doi:10.1007/978-981-99-2473-8_13 · Cited by 7
Horton, C., Liu, Y., Wang, J., Green, J., Tsyporin, J., Chen, B., & Wang, Z. A. (2023) · Stem Cell Reports · doi:10.1016/j.stemcr.2023.04.003 · Cited by 11
Hasebe, T., Fujimoto, K., Buchholz, D. R., & Ishizuya‐Oka, A. (2020) · General and Comparative Endocrinology · doi:10.1016/j.ygcen.2020.113441 · Cited by 7
A new gain‐of‐function mouse line to study the role of Wnt3a in development and disease
Chalamalasetty, R. B., Ajima, R., Garriock, R. J., Kennedy, M. W., Tessarollo, L., & Yamaguchi, T. P. (2016) · genesis · doi:10.1002/dvg.22959 · Cited by 6
Active β-Catenin Signaling in the Small Intestine of Humans During Infancy
Dudhwala, Z. M., Drew, P. A., Howarth, G. S., Moore, D., & Cummins, A. G. (2018) · Digestive Diseases and Sciences · doi:10.1007/s10620-018-5286-y · Cited by 6
Cre-Recombinase Induces Apoptosis and Cell Death in Enterocyte Organoids
Moll, F., Spaeth, M., & Schröder, K. (2022) · Antioxidants · doi:10.3390/antiox11081452 · Cited by 6
Cheng, J., Tsuda, M., Okolotowicz, K. J., Dwyer, M., Bushway, P., Colas, A. R., Lancman, J. J., Schade, D., Perea‐Gil, I., Bruyneel, A. A. N., et al. (2021) · Cell chemical biology · doi:10.1016/j.chembiol.2021.01.001 · Cited by 7
Real‐Time Analysis of Endogenous Wnt Signalling in 3D Mesenchymal Stromal Cells
Saleh, F., Carstairs, A., Etheridge, S. L., & Genever, P. G. (2016) · Stem Cells International · doi:10.1155/2016/7132529 · Cited by 6
Notch inhibition counteracts Paneth cell death in absence of caspase-8
Jeon, M., Kaemmerer, E., Schneider, U., Schiffer, M., Klaus, C., Hennings, J., Clahsen, T., Ackerstaff, T., Niggemann, M., Schippers, A., et al. (2018) · Archiv für Pathologische Anatomie und Physiologie und für Klinische Medicin · doi:10.1007/s00428-018-2368-3 · Cited by 7
Chen, Z., Qi, H., Xue, Y., Zhang, Y., Zhang, Z., Xu, S., Liao, S., Zeng, X., Wu, J., Lv, X., et al. (2025) · MedComm – Oncology · doi:10.1002/mog2.70015 · Cited by 6
Insights from tissue “omics” analysis on intestinal remodeling in celiac disease
Stamnæs, J. (2021) · PROTEOMICS · doi:10.1002/pmic.202100057 · Cited by 6
Dickkopf1 induces enteric neurogenesis and gliogenesis in vitro if apoptosis is evaded
Scharr, M., Scherer, S., Hirt, B., & Neckel, P. H. (2023) · Communications Biology · doi:10.1038/s42003-023-05072-x · Cited by 5
Enteroendocrine cells switch hormone expression along the crypt-to-villus BMP signalling gradient
Beumer, J., Artegiani, B., Post, Y., Reimann, F. M., Gribble, F. M., Nguyen, T. N., Zeng, H., Van, D. B. M., Van, E. J. H., & Clevers, H. C. (2019) · Yearbook of pediatric endocrinology · doi:10.1530/ey.16.15.14 · Cited by 6
Self-citationLymphatics constitute a novel component of the intestinal stem cell nichePreprint
Goto, N., Imada, S., Deshpande, V., & Yilmaz, Ö. H. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.01.28.478205 · Cited by 5
Breastfeeding lifespan control of growth, maintenance, and metabolism of small intestinal epithelium
da Costa, A. V., Rattes, I. C., Goes, C. P., Lobo, L. H. G., Barreto, L. B. E., & Gama, P. (2023) · Journal of Cellular Physiology · doi:10.1002/jcp.31089 · Cited by 4
A flexible strategy to fabricate trumpet-shaped porous PDMS membranes for organ-on-chip application
Xie, Y., Guo, Y., Xie, F., Dong, Y., Zhang, X., Li, X., & Zhang, X. (2024) · Biomicrofluidics · doi:10.1063/5.0227148 · Cited by 4
Bose, A., & Visweswariah, S. S. (2025) · Biochemical Journal · doi:10.1042/bcj20253055 · Cited by 5
Erkert, L., Haag, L.-M., & Becker, C. (2026) · Seminars in Immunopathology · doi:10.1007/s00281-026-01068-x · Cited by 5
Patterning human kidney organoids with synthetic Wnt-secreting organizers
Fausto, C. C., Glykofrydis, F., Kumar, N., Schnell, J., Csipán, R. L., Kuyper, F. D., Kunnan, M., Grubbs, B. H., Thornton, M. E., Thompson, M., et al. (2026) · Science · doi:10.1126/science.adu9122 · Cited by 4
Hasebe, T., Fujimoto, K., & Ishizuya‐Oka, A. (2022) · Vitamins and hormones · doi:10.1016/bs.vh.2022.11.006 · Cited by 4
A Bio‐Functional Wnt3a Gradient Microarray for Cell Niche Studies
Wang, X., Ou, W., Feng, D., Gao, B., & Chan, B. P. (2023) · Advanced Functional Materials · doi:10.1002/adfm.202301941 · Cited by 4
Understanding and manipulating extracellular behaviors of Wnt ligands
Mii, Y. (2024) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-024-00856-9 · Cited by 4
Cancer systems biology: Live imaging of intestinal tissue in health and disease
Ponsioen, B., & Snippert, H. J. G. (2017) · Current Opinion in Systems Biology · doi:10.1016/j.coisb.2017.02.001 · Cited by 4
Bioartificial gut—current state of small intestinal tissue engineering
Däullary, T., Fey, C., Berger, C., Metzger, M., & Zdzieblo, D. (2020) · Elsevier eBooks · doi:10.1016/b978-0-08-102906-0.00025-8 · Cited by 4
Area patterning of the mammalian neocortex
Grove, E. A. (2020) · Elsevier eBooks · doi:10.1016/b978-0-12-814405-3.00003-5 · Cited by 4
Kurup, S., Tan, C., & Kume, T. (2023) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2023.1329770 · Cited by 4
Boswell, C. W., Hoppe, C., Sherrard, A., Miao, L., Kojima, M. L., Gopinath, S. K., Musaev, D., Zhao, N., Stasevich, T. J., & Giráldez, A. J. (2023) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2023.11.15.567075 · Cited by 4
Cell competition as an emerging mechanism and therapeutic target in cancer
Yang, D., Sun, W., Gao, L., Zhao, K., Zhuang, Q., & Cai, Y. (2025) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2025.167769 · Cited by 3
Secreted Frizzled-related proteins in animal development
Sadriev, K. (2025) · Histochemistry and Cell Biology · doi:10.1007/s00418-025-02415-z · Cited by 3
Metadata
- Authors
- Farin, H. F., Jordens, I., Mosa, M. H., Basak, O., Korving, J., Tauriello, D. V. F., de Punder, K., Angers, S., Peters, P. J., Maurice, M. M., & Clevers, H.
- Affiliations
- Royal Netherlands Academy of Arts and Sciences (NL)
- University Medical Center Utrecht (NL)
- German Cancer Research Center (DE)
- Maastricht University (NL)
- Corresponding author
- Farin, Henner F.
- Clevers, Hans
- ORCID
- Venue
- Nature
- Published
- 2016-02
- Volume
- 530
- Issue
- 7590
- Pages
- 340-343
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2258083311
- Cited by
- 525
- References
- 38
- Citations by year
- 2026: 27 · 2025: 41 · 2024: 31 · 2023: 48 · 2022: 47 · 2021: 46 · 2020: 55 · 2019: 56 · 2018: 76 · 2017: 62 · 2016: 35
- FWCI
- 39.70
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- KNAW Research Portal (The Royal Netherlands Academy of Arts and Sciences) (publishedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- Research Publications (Maastricht University) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Wnt/β-catenin signaling in development and cancer
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Hedgehog Signaling Pathway Studies
- Keywords
- Wnt3, Wnt signaling, intestinal stem cells, Paneth cells, short-range signaling, intestinal crypts, intestinal organoids, Frizzled receptors, cell division, stem cell self-renewal
- MeSH terms
- Alleles, Animals, Cell Adhesion, Cell Division, Cell Membrane, Diffusion, Female, Intestinal Mucosa, Male, Organoids, Stem Cells, Paneth Cells, Intercellular Signaling Peptides and Proteins, Receptors, G-Protein-Coupled, Ubiquitin-Protein Ligases, Frizzled Receptors, Mice, Stem Cell Niche, Gene Knock-In Techniques, Wnt Signaling Pathway, Wnt3 Protein
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review