The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.
A small molecule enhances RNA interference and promotes microRNA processing
Open accessAbstract
Small interfering RNAs (siRNAs) and microRNAs (miRNAs) are sequence-specific post-transcriptional regulators of gene expression. Although major components of the RNA interference (RNAi) pathway have been identified, regulatory mechanisms for this pathway remain largely unknown. Here we demonstrate that the RNAi pathway can be modulated intracellularly by small molecules. We have developed a cell-based assay to monitor the activity of the RNAi pathway and find that the small-molecule enoxacin (Penetrex) enhances siRNA-mediated mRNA degradation and promotes the biogenesis of endogenous miRNAs. We show that this RNAi-enhancing activity depends on the trans-activation-responsive region RNA-binding protein. Our results provide a proof-of-principle demonstration that small molecules can be used to modulate the activity of the RNAi pathway. RNAi enhancers may be useful in the development of research tools and therapeutics.
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Cites
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TRBP recruits the Dicer complex to Ago2 for microRNA processing and gene silencing
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Cited by
Non-coding RNAs in human disease
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MicroRNA functions in stress responses
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MicroRNAs in neuronal function and dysfunction
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MicroRNAs as novel biological targets for detection and regulation
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Metadata
- Authors
- Shan, G., Li, Y., Zhang, J., Li, W., Szulwach, K. E., Duan, R., Faghihi, M. A., Khalil, A. M., Lu, L., Paroo, Z., Chan, A. W. S., Shi, Z., Liu, Q., Wahlestedt, C., He, C., & Jin, P.
- Venue
- Nature Biotechnology
- Published
- 2008-07-20
- Volume
- 26
- Issue
- 8
- Pages
- 933-940
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W2107101690
- Cited by
- 263
- References
- 28
- FWCI
- 4.59
- Type
- journal article
- Language
- en
- Open access
- green
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- Shan, G., Li, Y., Zhang, J., Li, W., Szulwach, K. E., Duan, R., Faghihi, M. A., Khalil, A. M., Lu, L., Paroo, Z., Chan, A. W. S., Shi, Z., Liu, Q., Wahlestedt, C., He, C., & Jin, P. (2008). A small molecule enhances RNA interference and promotes microRNA processing. Nature Biotechnology. https://doi.org/10.1038/nbt.1481
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