Cited by 3,620Pluripotent Stem Cells ResearchNeurogenesis and neuroplasticity mechanismsDevelopmental Biology and Gene Regulation

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Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling

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Chambers, Stuart M, Fasano, Christopher A, Papapetrou, Eirini P, Tomishima, Mark, Sadelain, Michel, Studer, Lorenz·2009·Nature Biotechnology

DOI 10.1038/nbt.1529PMC PMC2756723

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Abstract

Current neural induction protocols for human embryonic stem (hES) cells rely on embryoid body formation, stromal feeder co-culture or selective survival conditions. Each strategy has considerable drawbacks, such as poorly defined culture conditions, protracted differentiation and low yield. Here we report that the synergistic action of two inhibitors of SMAD signaling, Noggin and SB431542, is sufficient to induce rapid and complete neural conversion of >80% of hES cells under adherent culture conditions. Temporal fate analysis reveals the appearance of a transient FGF5(+) epiblast-like stage followed by PAX6(+) neural cells competent to form rosettes. Initial cell density determines the ratio of central nervous system and neural crest progeny. Directed differentiation of human induced pluripotent stem (hiPS) cells into midbrain dopamine and spinal motoneurons confirms the robustness and general applicability of the induction protocol. Noggin/SB431542-based neural induction should facilitate the use of hES and hiPS cells in regenerative medicine and disease modeling and obviate the need for protocols based on stromal feeders or embryoid bodies.

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Authors: Chambers, S. M., Fasano, C. A., Papapetrou, E. P., Tomishima, M., Sadelain, M., & Studer, L.
Affiliations: Memorial Sloan Kettering Cancer Center (US)
Corresponding author: Chambers, Stuart M
Studer, Lorenz
ORCID: Chambers, Stuart M
Papapetrou, Eirini P
Sadelain, Michel
Studer, Lorenz
Venue: Nature Biotechnology
Publisher: Springer Science and Business Media LLC
Published: 2009-03
Volume: 27
Issue: 3
Pages: 275-280
ISSN: 0733-222X (ISSN-L)
1087-0156
1546-1696
DOI: 10.1038/nbt.1529
PMC: PMC2756723
OpenAlex: W2087786585
Cited by: 3,620
References: 24
Citations by year: 2026: 155 · 2025: 211 · 2024: 225 · 2023: 241 · 2022: 229 · 2021: 287 · 2020: 292 · 2019: 238 · 2018: 241 · 2017: 235 · 2016: 240 · 2015: 225 · 2014: 185 · 2013: 199 · 2012: 184
FWCI: 55.89
Type: journal article
Language: en
Update notices: erratum (2009-05-01)
Open access: green
www.ncbi.nlm.nih.gov
Licence: www.springer.com/tdm (tdm)
www.springer.com/tdm (vor)
Locations: Nature Biotechnology (publishedVersion)
PubMed (publishedVersion)
PubMed Central (acceptedVersion)
Funders: National Institute of Neurological Disorders and Stroke (R01NS052671)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords: neuronal differentiation, human embryonic stem cells, induced pluripotent stem cells, noggin, SB431542, epiblast-like cells, Pax6, rosette formation, midbrain dopaminergic neurons, spinal motoneurons, adherent culture
MeSH terms: Noggin Protein, Carrier Proteins, Cell Differentiation, Humans, Mesencephalon, Neurons, Signal Transduction, Cell Culture Techniques, Pluripotent Stem Cells, Smad Proteins, Embryonic Stem Cells

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Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling

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