Monoclonal antibodies isolated without screening by analyzing the variable-gene repertoire of plasma cells

Continuous cultures of fused cells secreting antibody of predefined specificity

Köhler, G., & Milstein, C. (1975) · Nature · doi:10.1038/256495a0 · Cited by 17,200

Next-generation DNA sequencing

Shendure, J., & Ji, H. (2008) · Nature Biotechnology · doi:10.1038/nbt1486 · Cited by 4,509

IMGT/V-QUEST: the highly customized and integrated system for IG and TR standardized V-J and V-D-J sequence analysis

Brochet, X., Lefranc, M., & Giudicelli, V. (2008) · Nucleic Acids Research · doi:10.1093/nar/gkn316 · Cited by 1,220

Making antibody fragments using phage display libraries

Clackson, T., Hoogenboom, H. R., Griffiths, A. D., & Winter, G. P. (1991) · Nature · doi:10.1038/352624a0 · Cited by 1,211

Competence and competition: the challenge of becoming a long-lived plasma cell

Radbruch, A., Muehlinghaus, G., Luger, E., Inamine, A., Smith, K. G. C., Dörner, T., & Hiepe, F. (2006) · Nature reviews. Immunology · doi:10.1038/nri1886 · Cited by 1,033

Rapid cloning of high-affinity human monoclonal antibodies against influenza virus

Wrammert, J., Smith, K., Miller, J., Langley, W. A., Kokko, K. E., Larsen, C. P., Zheng, N., Mays, I., Garman, L., Helms, C., et al. (2008) · Nature · doi:10.1038/nature06890 · Cited by 1,026

Selecting and screening recombinant antibody libraries

Hoogenboom, H. R. (2005) · Nature Biotechnology · doi:10.1038/nbt1126 · Cited by 975

Regulation of plasma-cell development

Shelef, M. A., & Calame, K. L. (2005) · Nature reviews. Immunology · doi:10.1038/nri1572 · Cited by 917

Lifetime of plasma cells in the bone marrow

Manz, R. A., Thiel, A., & Radbruch, A. (1997) · Nature · doi:10.1038/40540 · Cited by 866

An efficient method to make human monoclonal antibodies from memory B cells: potent neutralization of SARS coronavirus

Traggiai, E., Becker, S., Subbarao, K., Kolesnikova, L., Uematsu, Y., Gismondo, M. R., Murphy, B. R., Rappuoli, R., & Lanzavecchia, A. (2004) · Nature Medicine · doi:10.1038/nm1080 · Cited by 697

Forecasting of emerging therapeutic monoclonal antibodies patents based on a decision model

Pereira, C. G., Lavoie, J. R., Garces, E., Basso, F. G., Dabić, M., Porto, G. S., & Daim, T. U. (2018) · Technological Forecasting and Social Change · doi:10.1016/j.techfore.2018.11.002 · Cited by 40

Antibody Repertoire Analysis of Hepatitis C Virus Infections Identifies Immune Signatures Associated With Spontaneous Clearance

Eliyahu, S., Sharabi, O., Elmedvi, S., Timor, R., Davidovich, A., Vigneault, F., Clouser, C., Hope, R., Assy, N., Braun, M., et al. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.03004 · Cited by 37

Identifying T Cell Receptors from High-Throughput Sequencing: Dealing with Promiscuity in TCRα and TCRβ Pairing

Lee, E. S., Thomas, P. G., Mold, J. E., & Yates, A. J. (2017) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1005313 · Cited by 39

Rare, high-affinity mouse anti-PD-1 antibodies that function in checkpoint blockade, discovered using microfluidics and molecular genomics

Adler, A. S., Mizrahi, R. A., Spindler, M. J., Adams, M. S., Asensio, M. A., Edgar, R. C., Leong, J., Leong, R., & Johnson, D. S. (2017) · mAbs · doi:10.1080/19420862.2017.1371386 · Cited by 40

Applications of Machine and Deep Learning in Adaptive Immunity

Pertseva, M., Gao, B., Neumeier, D., Yermanos, A., & Reddy, S. T. (2021) · Annual Review of Chemical and Biomolecular Engineering · doi:10.1146/annurev-chembioeng-101420-125021 · Cited by 38

Approaches to expand the conventional toolbox for discovery and selection of antibodies with drug-like physicochemical properties

Svilenov, H. L., Arosio, P., Menzen, T., Tessier, P. M., & Sormanni, P. (2023) · mAbs · doi:10.1080/19420862.2022.2164459 · Cited by 40

From deep sequencing to actual clones

D’Angelo, S., Kumar, S., Naranjo, L. A., Ferrara, F., Kiss, C., & Bradbury, A. M. (2014) · Protein Engineering Design and Selection · doi:10.1093/protein/gzu032 · Cited by 38

Benchmarking Tree and Ancestral Sequence Inference for B Cell Receptor Sequences

Davidsen, K., & Matsen, F. A., IV (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.02451 · Cited by 35

An array of 60,000 antibodies for proteome-scale antibody generation and target discovery

Wang, Z., Li, Y., Hou, B., Pronobis, M. I., Wang, M., Wang, Y., Cheng, G., Zhang, Z., Wang, Y., Tang, Y., et al. (2020) · Science Advances · doi:10.1126/sciadv.aax2271 · Cited by 39

A computational method for immune repertoire mining that identifies novel binders from different clonotypes, demonstrated by identifying anti-pertussis toxoid antibodies

Richardson, E., Galson, J. D., Kellam, P., Kelly, D. F., Smith, S. E., Palser, A. L., Watson, S. J., & Deane, C. M. (2021) · mAbs · doi:10.1080/19420862.2020.1869406 · Cited by 41

Intrinsic bias and public rearrangements in the human immunoglobulin Vλ light chain repertoire

Hoi, K. H., & Ippolito, G. C. (2013) · Genes and Immunity · doi:10.1038/gene.2013.10 · Cited by 37

Structure-based cross-docking analysis of antibody–antigen interactions

Kilambi, K. P., & Gray, J. J. (2017) · Scientific Reports · doi:10.1038/s41598-017-08414-y · Cited by 37

Nanoscale integration of single cell biologics discovery processes using optofluidic manipulation and monitoring

Jorgolli, M., Nevill, T., Winters, A. G., Chen, I., Chong, S., Lin, F., Mock, M. L., Chen, C., Le, K., Tan, C., et al. (2019) · Biotechnology and Bioengineering · doi:10.1002/bit.27024 · Cited by 35

Recombinant outer membrane protein A induces a protective immune response against Escherichia coli infection in mice

Guan, Q., Wang, X., Wang, X., Teng, D., Mao, R., Zhang, Y., & Wang, J. (2015) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-014-6339-6 · Cited by 37

Isolation and characterization of antigen-specific alpaca (Lama pacos) VHH antibodies by biopanning followed by high-throughput sequencing

Miyazaki, N., Kiyose, N., Akazawa, Y., Takashima, M., Hagihara, Y., Inoue, N., Matsuda, T., Ogawa, R., Inoue, S., & Ito, Y. (2015) · The Journal of Biochemistry · doi:10.1093/jb/mvv038 · Cited by 36

Exploiting Highly Ordered Subnanoliter Volume Microcapillaries as Microtools for the Analysis of Antibody Producing Cells

Fitzgerald, V., Manning, B., O’Donnell, B., O’Reilly, B., O’Sullivan, D., O’Kennedy, R. J., & Leonard, P. (2014) · Analytical Chemistry · doi:10.1021/ac503547j · Cited by 36

De novo design of antibody complementarity determining regions binding a FLAG tetra-peptide

Entzminger, K. C., Hyun, J., Pantazes, R. J., Patterson-Orazem, A. C., Qerqez, A. N., Frye, Z., Hughes, R. A., Ellington, A. D., Lieberman, R. L., Maranas, C. D., et al. (2017) · Scientific Reports · doi:10.1038/s41598-017-10737-9 · Cited by 36

The Application of Next Generation Sequencing to the Understanding of Antibody Repertoires

Mathonet, P., & Ullman, C. G. (2013) · Frontiers in Immunology · doi:10.3389/fimmu.2013.00265 · Cited by 36

Antibody repertoire analysis of mouse immunization protocols using microfluidics and molecular genomics

Asensio, M. A., Lim, Y. W., Wayham, N. P., Stadtmiller, K., Edgar, R. C., Leong, J., Leong, R., Mizrahi, R. A., Adams, M. S., Simons, J. F., et al. (2019) · mAbs · doi:10.1080/19420862.2019.1583995 · Cited by 34

IgG variable region and VH CDR3 diversity in unimmunized mice analyzed by massively parallel sequencing

Lü, J., Panavas, T., Thys, K., Aerssens, J., Naso, M. F., Fisher, J., Rycyzyn, M. A., & Sweet, R. W. (2013) · Molecular Immunology · doi:10.1016/j.molimm.2013.09.008 · Cited by 33

Synthetic Standards Combined With Error and Bias Correction Improve the Accuracy and Quantitative Resolution of Antibody Repertoire Sequencing in Human Naïve and Memory B Cells

Friedensohn, S., Lindner, J. M., Cornacchione, V., Iazeolla, M., Miho, E., Zingg, A., Meng, S. M., Traggiai, E., & Reddy, S. T. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.01401 · Cited by 33

Antibody discovery and engineering by enhanced CRISPR-Cas9 integration of variable gene cassette libraries in mammalian cells

Parola, C., Neumeier, D., Friedensohn, S., Csepregi, L., Tacchio, M. D., Mason, D. M., & Reddy, S. T. (2019) · mAbs · doi:10.1080/19420862.2019.1662691 · Cited by 33

Frontier of therapeutic antibody discovery: The challenges and how to face them

Lu, Z. (2012) · World Journal of Biological Chemistry · doi:10.4331/wjbc.v3.i12.187 · Cited by 30

Discovery of high affinity anti-ricin antibodies by B cell receptor sequencing and by yeast display of combinatorial VH:VLlibraries from immunized animals

Wang, B., Lee, C., Johnson, E. L., Kluwe, C., Cunningham, J. C., Tanno, H., Crooks, R. M., Georgiou, G., & Ellington, A. D. (2016) · mAbs · doi:10.1080/19420862.2016.1190059 · Cited by 31

Expression Cloning and Production of Human Heavy-Chain-Only Antibodies from Murine Transgenic Plasma Cells

Drabek, D., Janssens, R., de Boer, E., Rademaker, R., Kloess, J., Skehel, J. J., & Grosveld, F. G. (2016) · Frontiers in Immunology · doi:10.3389/fimmu.2016.00619 · Cited by 31

Fast, accurate antibody structure prediction from deep learning on massive set of natural antibodiesPreprint

Ruffolo, J. A., Chu, L., Mahajan, S. P., & Gray, J. J. (2022) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2022.04.20.488972 · Cited by 33

Pacific Biosciences Sequencing and IMGT/HighV-QUEST Analysis of Full-Length Single Chain Fragment Variable from an In Vivo Selected Phage-Display Combinatorial Library

Hémadou, A., Giudicelli, V., Smith, M. L., Lefranc, M., Duroux, P., Κοσσίδα, Σ., Heiner, C. R., Hepler, N. L., Kuijpers, J., Groppi, A., et al. (2017) · Frontiers in Immunology · doi:10.3389/fimmu.2017.01796 · Cited by 29

Antibody-based nanotechnology

Hillman, Y., Lustiger, D., & Wine, Y. (2019) · Nanotechnology · doi:10.1088/1361-6528/ab12f4 · Cited by 29

The role of structural proteomics in vaccine development: recent advances and future prospects

Donnarumma, D., Faleri, A., Costantino, P., Rappuoli, R., & Norais, N. (2015) · Expert Review of Proteomics · doi:10.1586/14789450.2016.1121113 · Cited by 30

Profiling Germinal Center-like B Cell Responses to Conjugate Vaccines Using Synthetic Immune Organoids

Moeller, T. D., Shah, S. B., Lai, K., Lopez‐Barbosa, N., Desai, P., Wang, W., Zhong, Z., Redmond, D., Singh, A. K., & DeLisa, M. P. (2023) · ACS Central Science · doi:10.1021/acscentsci.2c01473 · Cited by 28

Identification of highly selective MMP‐14 inhibitory Fabs by deep sequencing

Lopez, T., Nam, D. H., Kaihara, E., Mustafa, Z., & Ge, X. (2017) · Biotechnology and Bioengineering · doi:10.1002/bit.26248 · Cited by 29

Improved computational epitope profiling using structural models identifies a broader diversity of antibodies that bind to the same epitope

Spoendlin, F. C., Abanades, B., Raybould, M. I. J., Wong, W. K., Georges, G., & Deane, C. M. (2023) · Frontiers in Molecular Biosciences · doi:10.3389/fmolb.2023.1237621 · Cited by 26

Systems analysis of adaptive immunity by utilization of high-throughput technologies

Reddy, S. T., & Georgiou, G. (2011) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2011.04.015 · Cited by 26

Escherichia coli outer membrane protein F (OmpF): an immunogenic protein induces cross-reactive antibodies against Escherichia coli and Shigella

Wang, X., Teng, D., Guan, Q., Mao, R., Hao, Y., Wang, X., Yao, J., & Wang, J. (2017) · AMB Express · doi:10.1186/s13568-017-0452-8 · Cited by 25

Transferring the Characteristics of Naturally Occurring and Biased Antibody Repertoires to Human Antibody Libraries by Trapping CDRH3 Sequences

Venet, S., Ravn, U., Buatois, V., Gueneau, F., Calloud, S., Kosco‐Vilbois, M. H., & Fischer, N. (2012) · PLoS ONE · doi:10.1371/journal.pone.0043471 · Cited by 24

Phenotypic determinism and stochasticity in antibody repertoires of clonally expanded plasma cells

Neumeier, D., Yermanos, A., Agrafiotis, A., Csepregi, L., Chowdhury, T., Ehling, R. A., Kuhn, R., Cotet, T., Roberto, R. B. D., Tacchio, M. D., et al. (2022) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2113766119 · Cited by 24

Impact of new sequencing technologies on studies of the human B cell repertoire

Finn, J. A., & Crowe, J. E. (2013) · Current Opinion in Immunology · doi:10.1016/j.coi.2013.09.010 · Cited by 23

Large-scale analysis of 2,152 Ig-seq datasets reveals key features of B cell biology and the antibody repertoire

Yang, X., Wang, M., Wu, J., Shi, D., Zhang, Y., Zeng, H., Zhu, Y., Lan, C., Deng, Y., Guo, S., et al. (2021) · Cell Reports · doi:10.1016/j.celrep.2021.109110 · Cited by 23

Comprehensive analysis of the T-cell receptor beta chain gene in rhesus monkey by high throughput sequencing

Li, Z., Liu, G., Tong, Y., Zhang, M., Xu, Y., Li, Q., Wang, Z., Chen, X., & He, J. (2015) · Scientific Reports · doi:10.1038/srep10092 · Cited by 22

Application of germline antibody features to vaccine development, antibody discovery, antibody optimization and disease diagnosis

Zhang, Y., Li, Q., Luo, L., Duan, C., Shen, J., & Wang, Z. (2023) · Biotechnology Advances · doi:10.1016/j.biotechadv.2023.108143 · Cited by 23

Immunological principles regulating immunomodulation with biomaterials

Khan, T. A., & Reddy, S. T. (2013) · Acta Biomaterialia · doi:10.1016/j.actbio.2013.12.011 · Cited by 22

Development of Antibody-Based Therapeutics

Tabrizi, M. A., Klakamp, S. L., & Bornstein, G. G. (2012) · Springer eBooks · doi:10.1007/978-1-4419-5955-3 · Cited by 23

High-throughput retrieval of physical DNA for NGS-identifiable clones in phage display library

Noh, J., Kim, O., Jung, Y., Han, H., Kim, J., Kim, S., Lee, S., Park, J., Jung, R. H., Kim, S. I., et al. (2019) · mAbs · doi:10.1080/19420862.2019.1571878 · Cited by 22

ASAP - A Webserver for Immunoglobulin-Sequencing Analysis Pipeline

Avram, O., Vaisman‐Mentesh, A., Yehezkel, D., Ashkenazy, H., Pupko, T., & Wine, Y. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.01686 · Cited by 21

SARS-CoV-2 reactive and neutralizing antibodies discovered by single-cell sequencing of plasma cells and mammalian display

Ehling, R. A., Weber, C. R., Mason, D. M., Friedensohn, S., Wagner, B., Bieberich, F., Kapetanovic, E., Vazquez-Lombardi, R., Roberto, R. B. D., Hong, K., et al. (2021) · Cell Reports · doi:10.1016/j.celrep.2021.110242 · Cited by 27

Exploring the impact of clonal definition on B-cell diversity: implications for the analysis of immune repertoires

Pélissier, A., Luo, S., Stratigopoulou, M., Guikema, J. E. J., & Martínez, M. R. (2023) · Frontiers in Immunology · doi:10.3389/fimmu.2023.1123968 · Cited by 21

The dengue-specific immune response and antibody identification with machine learning

Natali, E. N., Horst, A., Meier, P., Greiff, V., Nuvolone, M., Babrak, L. M., Fink, K., & Miho, E. (2024) · npj Vaccines · doi:10.1038/s41541-023-00788-7 · Cited by 21

Characterization of human IgG repertoires in an acute HIV-1 infection

Chen, W., Prabakaran, P., Zhu, Z., Feng, Y., Streaker, E. D., & Dimitrov, D. S. (2012) · Experimental and Molecular Pathology · doi:10.1016/j.yexmp.2012.09.022 · Cited by 21

Discovery of Novel Candidate Therapeutics and Diagnostics Based on Engineered Human Antibody Domains

Chen, W., Gong, R., Ying, T., Prabakaran, P., Zhu, Z., Feng, Y., & Dimitrov, D. S. (2014) · Current Drug Discovery Technologies · doi:10.2174/15701638113109990032 · Cited by 21

A Shorter Route to Antibody Binders via Quantitative in vitro Bead-Display Screening and Consensus Analysis

Mankowska, S. A., Gatti‐Lafranconi, P., Chodorge, M., Sridharan, S., Minter, R., & Hollfelder, F. (2016) · Scientific Reports · doi:10.1038/srep36391 · Cited by 20

Identification of high affinity HER2 binding antibodies using CHO Fab surface display

Nguyen, A. W., Le, K. C., & Maynard, J. A. (2018) · Protein Engineering Design and Selection · doi:10.1093/protein/gzy004 · Cited by 21

Predominant structural configuration of natural antibody repertoires enables potent antibody responses against protein antigens

Chen, H.-S., Hou, S.-C., Jian, J.-W., Goh, K.-S., Shen, S.-T., Lee, Y., You, J.-J., Peng, H., Kuo, W., Chen, S., et al. (2015) · Scientific Reports · doi:10.1038/srep12411 · Cited by 21

Recent advances using FcRn overexpression in transgenic animals to overcome impediments of standard antibody technologies to improve the generation of specific antibodies

Kacskovıcs, I., Cervenak, J., Erdei, A., Goldsby, R. A., & Butler, J. E. (2011) · mAbs · doi:10.4161/mabs.3.5.17023 · Cited by 20

Systems Biology Approach for New Target and Biomarker Identification

Wang, I., Stone, D. J., Nickle, D. C., Loboda, A. P., Puig, Ó., & Roberts, C. (2012) · Current topics in microbiology and immunology · doi:10.1007/82_2012_252 · Cited by 19

Fine epitope signature of antibody neutralization breadth at the HIV-1 envelope CD4-binding site

Cheng, H. D., Grimm, S. K., Gilman, M. S. A., Gwom, L. C., Sok, D., Sundling, C., Donofrio, G. C., Hedestam, G. B. K., Bonsignori, M., Haynes, B. F., et al. (2018) · JCI Insight · doi:10.1172/jci.insight.97018 · Cited by 19

Describing the diversity of Ag specific receptors in vertebrates: Contribution of repertoire deep sequencing

Castro, R., Navelsaker, S., Krasnov, A., Pasquier, L. D., & Boudinot, P. (2017) · Developmental & Comparative Immunology · doi:10.1016/j.dci.2017.02.018 · Cited by 17

Clonal array profiling of scFv-displaying phages for high-throughput discovery of affinity-matured antibody mutants

Kiguchi, Y., Oyama, H., Morita, I., Morikawa, M., Nakano, A., Fujihara, W., Inoue, Y., Sasaki, M., Saijo, Y., Kanemoto, Y., et al. (2020) · Scientific Reports · doi:10.1038/s41598-020-71037-3 · Cited by 18

A Novel and Effective Cancer Immunotherapy Mouse Model Using Antigen-Specific B Cells Selected In Vitro

Moutai, T., Yamana, H., Nojima, T., & Kitamura, D. (2014) · PLoS ONE · doi:10.1371/journal.pone.0092732 · Cited by 18

Metabolic Engineering of Saccharomyces cerevisiae for Production of Fragrant Terpenoids from Agarwood and Sandalwood

Promdonkoy, P., Sornlek, W., Preechakul, T., Tanapongpipat, S., & Runguphan, W. (2022) · Fermentation · doi:10.3390/fermentation8090429 · Cited by 18

Generation of a single-cell B cell atlas of antibody repertoires and transcriptomes to identify signatures associated with antigen specificity

Agrafiotis, A., Neumeier, D., Hong, K., Chowdhury, T., Ehling, R. A., Kuhn, R., Sandu, I., Kreiner, V., Cotet, T., Shlesinger, D., et al. (2023) · iScience · doi:10.1016/j.isci.2023.106055 · Cited by 15

Development of proteome‐wide binding reagents for research and diagnostics

Taussig, M., Schmidt, R., Cook, E. A., & Stoevesandt, O. (2013) · PROTEOMICS - CLINICAL APPLICATIONS · doi:10.1002/prca.201300060 · Cited by 17

Next-Generation DNA Sequencing of VH/VL Repertoires: A Primer and Guide to Applications in Single-Domain Antibody Discovery

Henry, K. A. (2017) · Methods in molecular biology · doi:10.1007/978-1-4939-7447-4_24 · Cited by 17

Simple paired heavy- and light-chain antibody repertoire sequencing using endoplasmic reticulum microsomes

Devulapally, P. R., Bürger, J., Mielke, T., Konthur, Z., Lehrach, H., Yaspo, M., Glökler, J., & Warnatz, H.-J. (2018) · Genome Medicine · doi:10.1186/s13073-018-0542-5 · Cited by 17

Comprehensive Assessment of Potential Multiple Myeloma Immunoglobulin Heavy Chain V-D-J Intraclonal Variation Using Massively Parallel Pyrosequencing

Tschumper, R. C., Asmann, Y. W., Hossain, A., Huddleston, P. M., Wu, X., Dispenzieri, A. A., Eckloff, B. W., & Jelinek, D. F. (2012) · Oncotarget · doi:10.18632/oncotarget.469 · Cited by 17

High‐throughput sequencing of immune repertoires in multiple sclerosis

Lossius, A., Johansen, J. N., Vartdal, F., & Holmøy, T. (2016) · Annals of Clinical and Translational Neurology · doi:10.1002/acn3.295 · Cited by 16

Barcoded sequencing workflow for high throughput digitization of hybridoma antibody variable domain sequences

Chen, Y., Kim, S. H., Shang, Y., Guillory, J., Stinson, J., Zhang, Q., Hötzel, I., & Hoi, K. H. (2018) · Journal of Immunological Methods · doi:10.1016/j.jim.2018.01.004 · Cited by 16

Antibody–antigen complex modelling in the era of immunoglobulin repertoire sequencing

Raybould, M. I. J., Wong, W. K., & Deane, C. M. (2019) · Molecular Systems Design & Engineering · doi:10.1039/c9me00034h · Cited by 14

Accelerating antibody discovery using transgenic animals overexpressing the neonatal Fc receptor as a result of augmented humoral immunity

Cervenak, J., Kurrle, R., & Kacskovıcs, I. (2015) · Immunological Reviews · doi:10.1111/imr.12364 · Cited by 16

Paving the way to construct a new vaccine against Escherichia coli from its recombinant outer membrane protein C via a murine model

Wang, X., Guan, Q., Wang, X., Teng, D., Mao, R., Yao, J., & Wang, J. (2015) · Process Biochemistry · doi:10.1016/j.procbio.2015.05.001 · Cited by 16

Single B Cell Cloning and Production of Rabbit Monoclonal Antibodies

Rashidian, J., & Lloyd, J. (2019) · Methods in molecular biology · doi:10.1007/978-1-4939-9853-1_23 · Cited by 16

Conserved heavy/light contacts and germline preferences revealed by a large-scale analysis of natively paired human antibody sequences and structural data

Dudzic, P., Chomicz, D., Bielska, W., Jaszczyszyn, I., Zieliński, M., Janusz, B., Wróbel, S., Pannérer, M.-M. L., Phillips, A., Prabakaran, P., et al. (2025) · Communications Biology · doi:10.1038/s42003-025-08388-y · Cited by 15

Advances in antibody discovery from human BCR repertoires

Xu, Z., Ismanto, H. S., Zhou, H., Saputri, D. S., Sugihara, F., & Standley, D. M. (2022) · Frontiers in Bioinformatics · doi:10.3389/fbinf.2022.1044975 · Cited by 18

A Microfluidic Chip for Screening and Sequencing of Monoclonal Antibody at a Single-Cell Level

Zhang, W., Li, Q., Jia, F., Hu, Z., & Wei, Z. (2021) · Analytical Chemistry · doi:10.1021/acs.analchem.1c00918 · Cited by 15

Trends in computational biology—2010

Mak, H. C. (2011) · Nature Biotechnology · doi:10.1038/nbt.1747 · Cited by 14

Fusion Cytokines IL-7-Linker-IL-15 Promote Mycobacterium Tuberculosis Subunit Vaccine to Induce Central Memory like T Cell-Mediated Immunity

Bai, C., Zhou, L., Tang, J. X., He, J., Han, J., Niu, H., & Zhu, B. (2020) · Vaccines · doi:10.3390/vaccines8040715 · Cited by 14

Large-scale antibody immune response mapping of splenic B cells and bone marrow plasma cells in a transgenic mouse model

Pan, X., Acevedo, S. N. L., Cuziol, C., Tavernier, E. D., Fahad, A. S., Longjam, P. S., Rao, S. P., Aguilera, D., Rezé, M., Bricault, C. A., et al. (2023) · Frontiers in Immunology · doi:10.3389/fimmu.2023.1137069 · Cited by 14

An elaborate landscape of the human antibody repertoire against enterovirus 71 infection is revealed by phage display screening and deep sequencing

Chen, Z., Ren, X., Yang, J., Dong, J., Xue, Y., Sun, L., Zhu, Y., & Jin, Q. (2016) · mAbs · doi:10.1080/19420862.2016.1267086 · Cited by 13

Landscape of infection enhancing antibodies in COVID-19 and healthy donors

Ismanto, H. S., Xu, Z., Saputri, D. S., Wilamowski, J., Li, S., Nugraha, D. K., Horiguchi, Y., Okada, M., Arase, H., & Standley, D. M. (2022) · Computational and Structural Biotechnology Journal · doi:10.1016/j.csbj.2022.11.001 · Cited by 13

The analysis of VH and VL genes repertoires of Fab library built from peripheral B cells of human rabies virus vaccinated donors

Houimel, M. (2014) · Human Immunology · doi:10.1016/j.humimm.2014.05.005 · Cited by 13

ScaffoldSeq: Software for characterization of directed evolution populations

Woldring, D., Holec, P. V., & Hackel, B. J. (2016) · Proteins Structure Function and Bioinformatics · doi:10.1002/prot.25040 · Cited by 12

Monitoring Phage Biopanning by Next-Generation Sequencing

Vaisman‐Mentesh, A., & Wine, Y. (2017) · Methods in molecular biology · doi:10.1007/978-1-4939-7447-4_26 · Cited by 12

Efficient Screening and Design of Variable Domain of Heavy Chain Antibody Ligands Through High Throughput Sequencing for Affinity Chromatography to Purify Fab Fragments

Rafique, A., Satake, K., Kishimoto, S., Khan, K. H., Kato, D., & Ito, Y. (2019) · Monoclonal Antibodies in Immunodiagnosis and Immunotherapy · doi:10.1089/mab.2019.0027 · Cited by 12

CellectSeq: In silico discovery of antibodies targeting integral membrane proteins combining in situ selections and next-generation sequencing

Kelil, A., Gallo, E., Banerjee, S., Adams, J., & Sidhu, S. S. (2021) · Communications Biology · doi:10.1038/s42003-021-02066-5 · Cited by 12

The Cloning and Expression of Human Monoclonal Antibodies: Implications for Allergen Immunotherapy

James, L. K. (2016) · Current Allergy and Asthma Reports · doi:10.1007/s11882-015-0588-z · Cited by 12

Optimizing the translational value of animal models of glomerulonephritis: insights from recent murine prototypes

Foster, M. H. (2016) · American Journal of Physiology-Renal Physiology · doi:10.1152/ajprenal.00275.2016 · Cited by 12

Characteristics of Plasmablast Repertoire in Chronically HIV-Infected Individuals for Immunoglobulin H and L Chain Profiled by Single-Cell Analysis

Liao, H., Li, S., Yu, Y., Yue, Y., Su, K., Zheng, Q., Jiang, N., & Zhang, Z. (2020) · Frontiers in Immunology · doi:10.3389/fimmu.2019.03163 · Cited by 12

Isolation of Monoclonal Antibodies with Predetermined Conformational Epitope Specificity

Sholukh, A. M., Mukhtar, M. M., Humbert, M., Essono, S., Watkins, J. D., Vyas, H. K., Shanmuganathan, V., Hemashettar, G., Kahn, M., Hu, S., et al. (2012) · PLoS ONE · doi:10.1371/journal.pone.0038943 · Cited by 12

Profiling the IgOme: Meeting the challenge

Weiss-Ottolenghi, Y., & Gershoni, J. M. (2013) · FEBS Letters · doi:10.1016/j.febslet.2013.11.005 · Cited by 11

Full-length single-cell BCR sequencing paired with RNA sequencing reveals convergent responses to pneumococcal vaccination

Morgan, D. M., Zhang, Y. J., Kim, J. H., Murillo, M., Singh, S., Loschko, J., Surendran, N., Sekulović, O., Feng, E., Shi, S., et al. (2024) · Communications Biology · doi:10.1038/s42003-024-06823-0 · Cited by 10

The rise of big data: deep sequencing-driven computational methods are transforming the landscape of synthetic antibody design

Gallo, E. (2024) · Journal of Biomedical Science · doi:10.1186/s12929-024-01018-5 · Cited by 13

Old questions, new tools: does next-generation sequencing hold the key to unraveling intestinal B-cell responses?

Pabst, O., Hazanov, H., & Mehr, R. (2014) · Mucosal Immunology · doi:10.1038/mi.2014.103 · Cited by 11

In Vivo VL-Targeted Microbial Superantigen Induced Global Shifts in the B Cell Repertoire

Grönwall, C., Pond, S. L. K., Young, J. A., & Silverman, G. J. (2012) · The Journal of Immunology · doi:10.4049/jimmunol.1200245 · Cited by 11

Deep immunoglobulin repertoire sequencing depicts a comprehensive atlas of spike-specific antibody lineages shared among COVID-19 convalescents

Yan, Q., Zhang, Y., Hou, R., Pan, W., Liang, H., Gao, X., Deng, W., Huang, X., Qu, L., Tang, C., et al. (2023) · Emerging Microbes & Infections · doi:10.1080/22221751.2023.2290841 · Cited by 9

A Novel Affinity Tag, ABTAG, and Its Application to the Affinity Screening of Single-Domain Antibodies Selected by Phage Display

Hussack, G., Baral, T. N., Baardsnes, J., van Faassen, H., Raphael, S., Henry, K. A., Zhang, J., & MacKenzie, C. R. (2017) · Frontiers in Immunology · doi:10.3389/fimmu.2017.01406 · Cited by 10

Animal immunization merges with innovative technologies: A new paradigm shift in antibody discovery

Prabakaran, P., Rao, S. P., & Wendt, M. (2021) · mAbs · doi:10.1080/19420862.2021.1924347 · Cited by 11

Strategies to Obtain Diverse and Specific Human Monoclonal Antibodies From Transgenic Animals

Brüggemann, M., Osborn, M. J., Ma, B., & Buelow, R. (2017) · Transplantation · doi:10.1097/tp.0000000000001702 · Cited by 9

Characterization of RBD-specific cross-neutralizing antibodies responses against SARS-CoV-2 variants from COVID-19 convalescents

Wang, Z., Li, D., Chen, Y., Sun, Y., Jin, C., Hu, C., Feng, Y., Su, J., Ren, L., Hao, Y., et al. (2023) · Frontiers in Immunology · doi:10.3389/fimmu.2023.1160283 · Cited by 8

Membrane-anchored stalk domain of influenza HA enhanced immune responses in mice

Gao, D., Chen, Y., Han, D., Qi, Q., Sun, X., Zhang, H., Feng, H., & Wang, M. (2017) · Microbial Pathogenesis · doi:10.1016/j.micpath.2017.11.025 · Cited by 9

Preferential Identification of Agonistic OX40 Antibodies by Using Cell Lysate to Pan Natively Paired, Humanized Mouse-Derived Yeast Surface Display Libraries

Medina‐Cucurella, A. V., Mizrahi, R. A., Asensio, M. A., Edgar, R. C., Leong, J., Leong, R., Lim, Y. W., Nelson, A. L., Niedecken, A. R., Simons, J. F., et al. (2019) · Antibodies · doi:10.3390/antib8010017 · Cited by 9

Antigen-Specific Human Monoclonal Antibodies from Transgenic Mice

Magadán, S., & González‐Fernández, Á. (2013) · Methods in molecular biology · doi:10.1007/978-1-62703-586-6_13 · Cited by 9

Using Next-Generation Sequencing for Discovery of High-Frequency Monoclonal Antibodies in the Variable Gene Repertoires from Immunized Mice

Haessler, U., & Reddy, S. T. (2014) · Methods in molecular biology · doi:10.1007/978-1-62703-992-5_12 · Cited by 8

News in brief

(2010) · Nature Methods · doi:10.1038/nmeth1110-871

Global survey of the immunoglobulin repertoire using next generation sequencing technology

Hoi, K. H. (2014) · Texas ScholarWorks (Texas Digital Library)

Generating antigen-specific antibodies through analysis of the variable-gene repertoire of plasma cells

(2010) · Science-Business eXchange · doi:10.1038/scibx.2010.1078

Identification of antigen-specific human monoclonal antibodies using high-throughput sequencing of the antibody repertoire

Liu, J., Li, R., Liu, K., Li, L., Zai, X., Chi, X., Fu, L. L., Xu, J., & Chen, W. (2016) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2016.03.038 · Cited by 5