An efficient method to make human monoclonal antibodies from memory B cells: potent neutralization of SARS coronavirus
Open accessAbstract
Passive serotherapy can confer immediate protection against microbial infection, but methods to rapidly generate human neutralizing monoclonal antibodies are not yet available. We have developed an improved method for Epstein-Barr virus transformation of human B cells. We used this method to analyze the memory repertoire of a patient who recovered from severe acute respiratory syndrome coronavirus (SARS-CoV) infection and to isolate monoclonal antibodies specific for different viral proteins, including 35 antibodies with in vitro neutralizing activity ranging from 10(-8)M to 10(-11)M. One such antibody confers protection in vivo in a mouse model of SARS-CoV infection. These results show that it is possible to interrogate the memory repertoire of immune donors to rapidly and efficiently isolate neutralizing antibodies that have been selected in the course of natural infection.
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Human neutralizing antibodies protected mice challenged with SARS-CoV and MERS-CoV.
“previous reports have shown that human neutralizing antibodies protected mice challenged with SARS-CoV (12–14) and Middle East respiratory syndrome (MERS)-CoV (15)”
From An <i>Alphavirus</i> -derived replicon RNA vaccine induces SARS-CoV-2 neutralizing antibody and T cell responses in mice and nonhuman primates (2020) · cites this paper as [14]
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- Authors
- Traggiai, E., Becker, S., Subbarao, K., Kolesnikova, L., Uematsu, Y., Gismondo, M. R., Murphy, B. R., Rappuoli, R., & Lanzavecchia, A.
- Affiliations
- Institute for Research in Biomedicine (CH)
- Philipps University of Marburg (DE)
- National Institutes of Health (US)
- Luigi Sacco Hospital (IT)
- Corresponding author
- Lanzavecchia, Antonio
- ORCID
- Venue
- Nature Medicine
- Published
- 2004-07-11
- Volume
- 10
- Issue
- 8
- Pages
- 871-875
- ISSN
- 1078-8956 (ISSN-L)
- 1546-170X
- PMC
- PMC7095806
- OpenAlex
- W1998383538
- Cited by
- 697
- References
- 31
- Citations by year
- 2026: 4 · 2025: 9 · 2024: 22 · 2023: 24 · 2022: 31 · 2021: 55 · 2020: 107 · 2019: 15 · 2018: 19 · 2017: 19 · 2016: 28 · 2015: 31 · 2014: 28 · 2013: 24 · 2012: 30
- FWCI
- 24.13
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Nature Medicine (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Helmut Horten Stiftung
- National Institute of Allergy and Infectious Diseases
- Topics
- Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
- Keywords
- SARS coronavirus, neutralizing monoclonal antibodies, memory B cells, Epstein-Barr virus transformation, antibody repertoire, in vivo protection
- MeSH terms
- Adult, alpha-Macroglobulins, Animals, Antibodies, Monoclonal, B-Lymphocytes, Chlorocebus aethiops, Disease Models, Animal, Enzyme-Linked Immunosorbent Assay, Herpesvirus 4, Human, Humans, Immunization, Passive, Immunologic Memory, Membrane Glycoproteins, Neutralization Tests, Transformation, Genetic, Vero Cells, Viral Envelope Proteins, Polymerase Chain Reaction, Microscopy, Immunoelectron, DNA Primers, Severe Acute Respiratory Syndrome, Mice, Spike Glycoprotein, Coronavirus
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