Cited by 697SARS-CoV-2 and COVID-19 ResearchMonoclonal and Polyclonal Antibodies Researchvaccines and immunoinformatics approaches

An efficient method to make human monoclonal antibodies from memory B cells: potent neutralization of SARS coronavirus

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Traggiai, Elisabetta, Becker, Stephan, Subbarao, Kanta, Kolesnikova, Larissa, Uematsu, Yasushi, Gismondo, Maria Rita, Murphy, Brian R, Rappuoli, Rino +1 more·2004·Nature Medicine

Abstract

Passive serotherapy can confer immediate protection against microbial infection, but methods to rapidly generate human neutralizing monoclonal antibodies are not yet available. We have developed an improved method for Epstein-Barr virus transformation of human B cells. We used this method to analyze the memory repertoire of a patient who recovered from severe acute respiratory syndrome coronavirus (SARS-CoV) infection and to isolate monoclonal antibodies specific for different viral proteins, including 35 antibodies with in vitro neutralizing activity ranging from 10(-8)M to 10(-11)M. One such antibody confers protection in vivo in a mouse model of SARS-CoV infection. These results show that it is possible to interrogate the memory repertoire of immune donors to rapidly and efficiently isolate neutralizing antibodies that have been selected in the course of natural infection.

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Authors
Traggiai, E., Becker, S., Subbarao, K., Kolesnikova, L., Uematsu, Y., Gismondo, M. R., Murphy, B. R., Rappuoli, R., & Lanzavecchia, A.
Affiliations
  • Institute for Research in Biomedicine (CH)
  • Philipps University of Marburg (DE)
  • National Institutes of Health (US)
  • Luigi Sacco Hospital (IT)
Corresponding author
Lanzavecchia, Antonio
Venue
Nature Medicine
Published
2004-07-11
Volume
10
Issue
8
Pages
871-875
ISSN
  • 1078-8956 (ISSN-L)
  • 1546-170X
OpenAlex
W1998383538
Cited by
697
References
31
Citations by year
2026: 4 · 2025: 9 · 2024: 22 · 2023: 24 · 2022: 31 · 2021: 55 · 2020: 107 · 2019: 15 · 2018: 19 · 2017: 19 · 2016: 28 · 2015: 31 · 2014: 28 · 2013: 24 · 2012: 30
FWCI
24.13
Type
journal article
Language
en
Open access
Locations
  • Nature Medicine (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Helmut Horten Stiftung
  • National Institute of Allergy and Infectious Diseases
Topics
  • Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 and COVID-19 Research
  • Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Monoclonal and Polyclonal Antibodies Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › vaccines and immunoinformatics approaches
Keywords
SARS coronavirus, neutralizing monoclonal antibodies, memory B cells, Epstein-Barr virus transformation, antibody repertoire, in vivo protection
MeSH terms
Adult, alpha-Macroglobulins, Animals, Antibodies, Monoclonal, B-Lymphocytes, Chlorocebus aethiops, Disease Models, Animal, Enzyme-Linked Immunosorbent Assay, Herpesvirus 4, Human, Humans, Immunization, Passive, Immunologic Memory, Membrane Glycoproteins, Neutralization Tests, Transformation, Genetic, Vero Cells, Viral Envelope Proteins, Polymerase Chain Reaction, Microscopy, Immunoelectron, DNA Primers, Severe Acute Respiratory Syndrome, Mice, Spike Glycoprotein, Coronavirus

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