A robust system for production of minicircle DNA vectors

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Improved Production and Purification of Minicircle DNA Vector Free of Plasmid Bacterial Sequences and Capable of Persistent Transgene Expression In Vivo

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An araC-controlled Bacterialcre Expression System to Produce DNA Minicircle Vectors for Nuclear and Mitochondrial Gene Therapy

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Long-term and homogeneous regulation of the Escherichia coli araBAD promoter by use of a lactose transporter of relaxed specificity

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Novel Minicircle Vector for Gene Therapy in Murine Myocardial Infarction

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Phage TP901-1 Site-Specific Integrase Functions in Human Cells

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Dual-In/Out strategy for genes integration into bacterial chromosome: a novel approach to step-by-step construction of plasmid-less marker-less recombinant E. coli strains with predesigned genome structure

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New Vector System for Random, Single-Step Integration of Multiple Copies of DNA into the Rhodococcus Genome

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Applications of genome editing technology in the targeted therapy of human diseases: mechanisms, advances and prospects

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In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration

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A Multifunctional AAV-CRISPR-Cas9 and Its Host Response

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Engineering CAR-T cells

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Niche stiffness underlies the ageing of central nervous system progenitor cells

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Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9

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Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases

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Overcoming Nonviral Gene Delivery Barriers: Perspective and Future

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Molecular mechanisms for enhanced DNA vaccine immunogenicity

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Hepatitis B Virus X Protein Promotes Degradation of SMC5/6 to Enhance HBV Replication

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Obligate Ligation-Gated Recombination (ObLiGaRe): Custom-designed nuclease-mediated targeted integration through nonhomologous end joiningPreprint

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Targeted Delivery of CRISPR/Cas9‐Mediated Cancer Gene Therapy via Liposome‐Templated Hydrogel Nanoparticles

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Enhanced CAR T-cell engineering using non-viral Sleeping Beauty transposition from minicircle vectors

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Viscous control of cellular respiration by membrane lipid composition

Budin, I., de Rond, T., Chen, Y., Chan, L. J. G., Petzold, C. J., & Keasling, J. D. (2018) · Science · doi:10.1126/science.aat7925 · Cited by 220 · see citing works

Resolving systematic errors in widely used enhancer activity assays in human cells

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Challenges and strategies for next-generation bispecific antibody-based antitumor therapeutics

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CRISPaint allows modular base-specific gene tagging using a ligase-4-dependent mechanism

Schmid-Burgk, J. L., Höning, K., Ebert, T. S., & Hornung, V. (2016) · Nature Communications · doi:10.1038/ncomms12338 · Cited by 190 · see citing works

The future of human DNA vaccines

Li, L., Saadé, F., & Petrovsky, N. (2012) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2012.08.012 · Cited by 180 · see citing works

Engineering DNA vaccines against infectious diseases

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Vector Design for Improved DNA Vaccine Efficacy, Safety and Production

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A Guide to Nucleic Acid Vaccines in the Prevention and Treatment of Infectious Diseases and Cancers: From Basic Principles to Current Applications

Qin, F., Xia, F., Chen, H., Cui, B., Feng, Y., Zhang, P., Chen, J., & Luo, M. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.633776 · Cited by 156 · see citing works

Engagement of the ATR-Dependent DNA Damage Response at the Human Papillomavirus 18 Replication Centers during the Initial Amplification

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Inhibition of HBV Transcription From cccDNA With Nitazoxanide by Targeting the HBx–DDB1 Interaction

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Minicircle DNA Vectors Achieve Sustained Expression Reflected by Active Chromatin and Transcriptional Level

Gracey, L. E., Maniar, J. M., Chen, Z.-Y., Lu, J., Fire, A. Z., & Kay, M. A. (2012) · Molecular Therapy · doi:10.1038/mt.2012.244 · Cited by 121 · see citing works

Engineering a far-red light–activated split-Cas9 system for remote-controlled genome editing of internal organs and tumors

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New Generation of Plasmid Backbones Devoid of Antibiotic Resistance Marker for Gene Therapy Trials

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Systematic evasion of the restriction-modification barrier in bacteria

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Core components of DNA lagging strand synthesis machinery are essential for hepatitis B virus cccDNA formation

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Gene therapy for monogenic liver diseases: clinical successes, current challenges and future prospects

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Hepatitis B virus cccDNA is formed through distinct repair processes of each strand

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Activation of melatonin receptor 2 but not melatonin receptor 1 mediates melatonin‐conferred cardioprotection against myocardial ischemia/reperfusion injury

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IL-17A gene transfer induces bone loss and epidermal hyperplasia associated with psoriatic arthritis

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Recombinant Adeno-Associated Viral Vectors (rAAV)-Vector Elements in Ocular Gene Therapy Clinical Trials and Transgene Expression and Bioactivity Assays

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Poly(2-ethyl-2-oxazoline)-PLA-g-PEI amphiphilic triblock micelles for co-delivery of minicircle DNA and chemotherapeutics

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Biomaterials as vectors for the delivery of CRISPR–Cas9

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Minicircle DNA vectors for gene therapy: advances and applications

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Expanding the scope of site‐specific recombinases for genetic and metabolic engineering

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Antibiotic-free selection in biotherapeutics: Now and forever

Mignon, C., Sodoyer, R., & Werle, B. (2015) · Pathogens · doi:10.3390/pathogens4020157 · Cited by 87 · see citing works

A comprehensive comparison of DNA and RNA vaccines

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Versatile Trans-Replication Systems for Chikungunya Virus Allow Functional Analysis and Tagging of Every Replicase Protein

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Nanotechnology and immunoengineering: How nanotechnology can boost CAR-T therapy

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HBVcircle: A novel tool to investigate hepatitis B virus covalently closed circular DNA

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Nanomedicine-based combination anticancer therapy between nucleic acids and small-molecular drugs

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Immune Cell Hacking: Challenges and Clinical Approaches to Create Smarter Generations of Chimeric Antigen Receptor T Cells

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Universal allogeneic CAR T cells engineered with Sleeping Beauty transposons and CRISPR-CAS9 for cancer immunotherapy

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Safety and efficacy of DNA vaccines

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Treatment of phenylketonuria using minicircle-based naked-DNA gene transfer to murine liver

Viecelli, H. M., Harbottle, R. P., Wong, S. P., Schlegel, A. A., Chuah, M., VandenDriessche, T., Harding, C. O., & Thöny, B. (2014) · Hepatology · doi:10.1002/hep.27104 · Cited by 74 · see citing works

Minicircle DNA Provides Enhanced and Prolonged Transgene Expression Following Airway Gene Transfer

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Converting extracellular vesicles into nanomedicine: loading and unloading of cargo

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Microvesicle-Mediated Delivery of Minicircle DNA Results in Effective Gene-Directed Enzyme Prodrug Cancer Therapy

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Derivation of Human Induced Pluripotent Stem Cells for Cardiovascular Disease Modeling

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Self-assembled magnetic theranostic nanoparticles for highly sensitive MRI of minicircle DNA delivery

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The Extragenic Spacer Length Between the 5′ and 3′ Ends of the Transgene Expression Cassette Affects Transgene Silencing From Plasmid-based Vectors

Lu, J., Zhang, F., Xu, S., Fire, A. Z., & Kay, M. A. (2012) · Molecular Therapy · doi:10.1038/mt.2012.65 · Cited by 64 · see citing works

DNA vaccines for prostate cancer

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Novel codon-optimized mini-intronic plasmid for efficient, inexpensive and xeno-free induction of pluripotency

Diecke, S., Lu, J., Lee, J., Termglinchan, V., Kooreman, N. G., Burridge, P. W., Ebert, A., Churko, J. M., Sharma, A., Kay, M. A., et al. (2015) · Scientific Reports · doi:10.1038/srep08081 · Cited by 62 · see citing works

Improving DNA Vaccine Performance Through Vector Design

Williams, J. (2014) · Current Gene Therapy · doi:10.2174/156652321403140819122538 · Cited by 62 · see citing works

Minicircle DNA is Superior to Plasmid DNA in Eliciting Antigen-specific CD8+ T-cell Responses

Dietz, W., Skinner, N., Hamilton, S. E., Jund, M. D., Heitfeld, S. M., Litterman, A. J., Hwu, P., Chen, Z.-Y., Salazar, A. Μ., Ohlfest, J. R., et al. (2013) · Molecular Therapy · doi:10.1038/mt.2013.85 · Cited by 62 · see citing works

Nonintegrating Knockdown and Customized Scaffold Design Enhances Human Adipose-Derived Stem Cells in Skeletal Repair

Lévi, B., Hyun, J. S., Nelson, E. R., Li, S., Montoro, D. T., Wan, D. C., Jia, F., Glotzbach, J. P., James, A. W., Lee, M., et al. (2011) · Stem Cells · doi:10.1002/stem.757 · Cited by 61 · see citing works

How Far Are Non-Viral Vectors to Come of Age and Reach Clinical Translation in Gene Therapy?

Sainz-Ramos, M., Gallego, I., Villate-Beitia, I., Sesma, J. Z., Maldonado, I., Puras, G., & Pedraz, J. L. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22147545 · Cited by 59 · see citing works

Comparative analysis of enzymatically produced novel linear DNA constructs with plasmids for use as DNA vaccines

Walters, A. A., Kinnear, E., Shattock, R. J., McDonald, J. U., Caproni, L. J., Porter, N., & Tregoning, J. S. (2014) · Gene Therapy · doi:10.1038/gt.2014.37 · Cited by 58 · see citing works

Vectorology and Factor Delivery in Induced Pluripotent Stem Cell Reprogramming

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Pevonedistat, a Neuronal Precursor Cell‐Expressed Developmentally Down‐Regulated Protein 8–Activating Enzyme Inhibitor, Is a Potent Inhibitor of Hepatitis B Virus

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A Mini-intronic Plasmid (MIP): A Novel Robust Transgene Expression Vector In Vivo and In Vitro

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Mapping of Betapapillomavirus Human Papillomavirus 5 Transcription and Characterization of Viral-Genome Replication Function

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Detecting cancers through tumor-activatable minicircles that lead to a detectable blood biomarker

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Mapping the Interactions of HBV cccDNA with Host Factors

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Non-viral therapeutic approaches to ocular diseases: An overview and future directions

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Managing MicroRNAs with Vector-Encoded Decoy-Type Inhibitors

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Vaccine development for bacterial pathogens: Advances, challenges and prospects

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Rational engineering of Escherichia coli strains for plasmid biopharmaceutical manufacturing

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The Cell Cycle Timing of Human Papillomavirus DNA Replication

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Merkel Cell Polyomavirus Encodes Circular RNAs (circRNAs) Enabling a Dynamic circRNA/microRNA/mRNA Regulatory Network

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In VivoElectroporation of Minicircle DNA as a Novel Method of Vaccine Delivery To Enhance HIV-1-Specific Immune Responses

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Cell Culture Models and Animal Models for HBV Study

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The recombined cccDNA produced using minicircle technology mimicked HBV genome in structure and function closely

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A Chikungunya Virus trans -Replicase System Reveals the Importance of Delayed Nonstructural Polyprotein Processing for Efficient Replication Complex Formation in Mosquito Cells

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Generation of minipigs with targeted transgene insertion by recombinase-mediated cassette exchange (RMCE) and somatic cell nuclear transfer (SCNT)

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Mammalian synthetic biology for studying the cell

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Calcium phosphate nanoneedle based gene delivery system for cancer genetic immunotherapy

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Etanercept-Synthesising Mesenchymal Stem Cells Efficiently Ameliorate Collagen-Induced Arthritis

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The march of pluripotent stem cells in cardiovascular regenerative medicine

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Efficacy of MRI visible iron oxide nanoparticles in delivering minicircle DNA into liver via intrabiliary infusion

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Minicircle microporation-based non-viral gene delivery improved the targeting of mesenchymal stem cells to an injury site

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Delivery of a sebum modulator by an engineered skin microbe in mice

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A Dual Target-directed Agent against Interleukin-6 Receptor and Tumor Necrosis Factor α ameliorates experimental arthritis

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An update on ACE2 amplification and its therapeutic potential

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Bacteriophage recombination systems and biotechnical applications

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From scaffold to structure: the synthetic production of cell derived extracellular matrix for liver tissue engineering

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Improving cell and gene therapy safety and performance using next-generation Nanoplasmid vectors

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DNA-Based Nonviral Gene Therapy─Challenging but Promising

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Macrophage-specific in vivo RNA editing promotes phagocytosis and antitumor immunity in mice

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Efficient Sleeping Beauty DNA Transposition From DNA Minicircles

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Micro-minicircle Gene Therapy: Implications of Size on Fermentation, Complexation, Shearing Resistance, and Expression

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DNA Ministrings: Highly Safe and Effective Gene Delivery Vectors

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Improved Minicircle DNA Biosynthesis for Gene Therapy Applications

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Towards effective non-viral gene delivery vector

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Gene engineered mesenchymal stem cells: greater transgene expression and efficacy with minicircle vs. plasmid DNA vectors in a mouse model of acute lung injury

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A Simple And Rapid Minicircle DNA Vector Manufacturing System

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Technical Report Improved Production and Purification of Minicircle DNA Vector Free of Plasmid Bacterial Sequences and Capable of Persistent Transgene Expression In Vivo

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Production of Minicircle DNA Vectors Using Site-Specific Recombinases

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Minicircle: an improved DNA molecule for in vitro and in vivo gene transfer

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