DNA targeting specificity of RNA-guided Cas9 nucleases

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Items 701–800 of 1,040

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2. Simple and Efficient Targeting of Multiple Genes Through CRISPR-Cas9 in Physcomitrella patens
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3. Precise and efficient scarless genome editing in stem cells using CORRECT
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4. In Vitro Enzymology of Cas9
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5. sgRNA Sequence Motifs Blocking Efficient CRISPR/Cas9-Mediated Gene Editing
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6. Covalent Modification of Bacteriophage T4 DNA Inhibits CRISPR-Cas9
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7. CRISPR/Cas9: A Tool to Circumscribe Cotton Leaf Curl Disease
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8. An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells
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9. A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system
   Martin W. LaFleur, Thao H. Nguyen, Matthew A. Coxe, Kathleen B. Yates, Justin D. Trombley, Sarah A. Weiss, Flavian D. Brown, Jacob E. Gillis, Daniel J. Coxe, John G. Doench et al. · 2019 · Nature Communications
10. Single-gene imaging links genome topology, promoter–enhancer communication and transcription control
    Jieru Li, Angela M. Hsu, Yujing Hua, Guanshi Wang, Lingling Cheng, Hiroshi Ochiai, Takashi Yamamoto, Alexandros Pertsinidis · 2020 · Nature Structural & Molecular Biology
11. Function genomics of abiotic stress tolerance in plants: a CRISPR approach
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12. CRISPR/Cas9-mediated genome engineering and the promise of designer flies on demand
    Scott J. Gratz, Jill Wildonger, Melissa M. Harrison, Kate M. O’Connor-Giles · 2013 · Fly
13. Allele-Specific CRISPR-Cas9 Genome Editing of the Single-Base P23H Mutation for Rhodopsin-Associated Dominant Retinitis Pigmentosa
    Pingjuan Li, Benjamin P. Kleinstiver, Mihoko Leon, Michelle S. Prew, Daniel Navarro-Gomez, Scott H. Greenwald, Eric A. Pierce, J. Keith Joung, Qin Liu · 2018 · The CRISPR Journal
14. Highly efficient genome editing via CRISPR–Cas9 in human pluripotent stem cells is achieved by transient BCL-XL overexpression
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15. Mutagenesis and homologous recombination in Drosophila cell lines using CRISPR/Cas9
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16. DNA-binding-domain fusions enhance the targeting range and precision of Cas9
    Mehmet Fatih Bolukbasi, Ankit Gupta, Sarah Oikemus, Alan G. Derr, Manuel G. Garber, Michael H Brodsky, Lihua Julie Zhu, Scot A. Wolfe · 2015 · Nature Methods
17. Fanconi Anemia Gene Editing by the CRISPR/Cas9 System
    Mark J. Osborn, Richard Gabriel, Beau R. Webber, Anthony P. DeFeo, Amber N. McElroy, Jordan Jarjour, Colby G. Starker, John E. Wagner, J. Keith Joung, Daniel F. Voytas et al. · 2014 · Human Gene Therapy
18. CRISPR/Cas9 Genome-Editing System in Human Stem Cells: Current Status and Future Prospects
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19. Hybridization Kinetics Explains CRISPR-Cas Off-Targeting Rules
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20. Stress resilience is promoted by a Zfp189-driven transcriptional network in prefrontal cortex
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21. Cas9-induced large deletions and small indels are controlled in a convergent fashion
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22. Efficient production of biallelic GGTA 1 knockout pigs by cytoplasmic microinjection of CRISPR /Cas9 into zygotes
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23. Zinc Fingers, TALEs, and CRISPR Systems: A Comparison of Tools for Epigenome Editing
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24. Base and nucleotide excision repair facilitate resolution of platinum drugs-induced transcription blockage
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25. HIV-specific humoral immune responses by CRISPR/Cas9-edited B cells
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26. CRISPR/Cas9-mediated genome engineering of CHO cell factories: application and perspectives
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27. Precision cancer mouse models through genome editing with CRISPR-Cas9
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28. Spell Checking Nature: Versatility of CRISPR/Cas9 for Developing Treatments for Inherited Disorders
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29. Hit-and-run epigenetic editing prevents senescence entry in primary breast cells from healthy donors
    Emily A. Saunderson, Peter Stepper, Jennifer J. Gomm, Lily Hoa, Adrienne Aiken Morgan, Michael D. Allen, J. Louise Jones, John G. Gribben, Tomasz Piotr Jurkowski, Gabriella Ficz · 2017 · Nature Communications
30. CRISPR/Cas9-Mediated Deletion of Large Genomic Fragments in Soybean
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31. SpRY greatly expands the genome editing scope in rice with highly flexible PAM recognition
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32. A Defined Zebrafish Line for High-Throughput Genetics and Genomics: NHGRI-1
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33. Genome editing using FACS enrichment of nuclease-expressing cells and indel detection by amplicon analysis
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34. Massively Parallel Biophysical Analysis of CRISPR-Cas Complexes on Next Generation Sequencing Chips
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35. Human genetic variation alters CRISPR-Cas9 on- and off-targeting specificity at therapeutically implicated loci
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36. Reduced mitochondrial fusion and Huntingtin levels contribute to impaired dendritic maturation and behavioral deficits in Fmr1-mutant mice
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37. Tools for experimental and computational analyses of off-target editing by programmable nucleases
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38. How specific is CRISPR/Cas9 really?
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39. Creating and evaluating accurate CRISPR-Cas9 scalpels for genomic surgery
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40. A Biophysical Model of CRISPR/Cas9 Activity for Rational Design of Genome Editing and Gene Regulation
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41. Functional significance of rare neuroligin 1 variants found in autism
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42. Precise Excision of the CAG Tract from the Huntingtin Gene by Cas9 Nickases
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43. Sharpening the Molecular Scissors: Advances in Gene-Editing Technology
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44. Using traditional machine learning and deep learning methods for on- and off-target prediction in CRISPR/Cas9: a review
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45. Genome Editing in Human Pluripotent Stem Cells: Approaches, Pitfalls, and Solutions
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46. A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells
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47. Editing the genome of hiPSC with CRISPR/Cas9: disease models
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48. Efficient CRISPR–Cas9 mediated multiplex genome editing in yeasts
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49. Prediction of CRISPR sgRNA Activity Using a Deep Convolutional Neural Network
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50. Integration of innate immune signalling by caspase-8 cleavage of N4BP1
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51. Sclerostin and Osteocalcin: Candidate Bone-Produced Hormones
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52. A Scalable Genome-Editing-Based Approach for Mapping Multiprotein Complexes in Human Cells
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53. Regulation of TET Protein Stability by Calpains
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54. A glance at genome editing with CRISPR–Cas9 technology
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55. Targeting Cdk11 in osteosarcoma cells using the CRISPR‐cas9 system
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56. Mouse Genome Engineering via CRISPR-Cas9 for Study of Immune Function
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57. FASTKD2 is an RNA-binding protein required for mitochondrial RNA processing and translation
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58. Site-specific genome editing for correction of induced pluripotent stem cells derived from dominant dystrophic epidermolysis bullosa
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59. Editing Transgenic DNA Components by Inducible Gene Replacement in Drosophila melanogaster
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60. Genome editing: the road of CRISPR/Cas9 from bench to clinic
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61. CRISPR/Cas9 in zebrafish: an efficient combination for human genetic diseases modeling
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62. CRISPR/Cas9-mediated multiple gene editing in Brassica oleracea var. capitata using the endogenous tRNA-processing system
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63. GPCR signaling inhibits mTORC1 via PKA phosphorylation of Raptor
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64. Strategies to Increase On-Target and Reduce Off-Target Effects of the CRISPR/Cas9 System in Plants
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65. The SPPL3-Defined Glycosphingolipid Repertoire Orchestrates HLA Class I-Mediated Immune Responses
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66. CRISPR-based technologies: prokaryotic defense weapons repurposed
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67. Functional genetics for all: engineered nucleases, CRISPR and the gene editing revolution
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68. Efficient Genome Editing in the Oomycete Phytophthora sojae Using CRISPR/Cas9
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69. Genome Editing Tools in Plants
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70. MAP4K3 mediates amino acid-dependent regulation of autophagy via phosphorylation of TFEB
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71. Advancements and Obstacles of CRISPR-Cas9 Technology in Translational Research
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72. Optimizing genome editing strategy by primer-extension-mediated sequencing
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73. Generation of heritable germline mutations in the jewel wasp Nasonia vitripennis using CRISPR/Cas9
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74. Locally Fixed Alleles: A method to localize gene drive to island populations
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75. Biodegradable Amino-Ester Nanomaterials for Cas9 mRNA Delivery in Vitro and in Vivo
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76. Cas9-Based Genome Editing in Xenopus tropicalis
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77. Profiling single-guide RNA specificity reveals a mismatch sensitive core sequence
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79. Loss of heterozygosity of essential genes represents a widespread class of potential cancer vulnerabilities
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80. CRISPR/Cas9-Enabled Multiplex Genome Editing and Its Application
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81. CRISPR‐Net: A Recurrent Convolutional Network Quantifies CRISPR Off‐Target Activities with Mismatches and Indels
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82. Patient-specific induced pluripotent stem cells (iPSCs) for the study and treatment of retinal degenerative diseases
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83. Progress in Genome Editing Technology and Its Application in Plants
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84. Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci
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85. Targeted genome engineering in human induced pluripotent stem cells from patients with hemophilia B using the CRISPR-Cas9 system
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86. Multi-faceted immunomodulatory and tissue-tropic clinical bacterial isolate potentiates prostate cancer immunotherapy
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87. Precise CRISPR/Cas9 editing of the NHE1 gene renders chickens resistant to the J subgroup of avian leukosis virus
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88. CRISPR/Cas9 gene editing for curing sickle cell disease
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89. Genome Editing Weds CRISPR: What Is in It for Phytoremediation?
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90. Resources for the design of CRISPR gene editing experiments
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91. CRISPR/Cas9: an advanced tool for editing plant genomes
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92. The Conformational Dynamics of Cas9 Governing DNA Cleavage Are Revealed by Single-Molecule FRET
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93. Improving CRISPR Genome Editing by Engineering Guide RNAs
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94. Harnessing Genome Editing Techniques to Engineer Disease Resistance in Plants
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95. Engineered CRISPR-OsCas12f1 and RhCas12f1 with robust activities and expanded target range for genome editing
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96. Enabling functional genomics with genome engineering
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97. Understanding and Editing the Zebrafish Genome
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98. CRISPR/Cas9 for Human Genome Engineering and Disease Research
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99. Different Effects of sgRNA Length on CRISPR-mediated Gene Knockout Efficiency
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100. Modulation of Host Learning in Aedes aegypti Mosquitoes
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