DNA targeting specificity of RNA-guided Cas9 nucleases

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Items 201–300 of 1,041

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18. WU-CRISPR: characteristics of functional guide RNAs for the CRISPR/Cas9 system
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19. Selection of chromosomal DNA libraries using a multiplex CRISPR system
    Owen W. Ryan, Jeffrey M. Skerker, Matthew J. Maurer, Xin Li, Jordan C. Tsai, Snigdha Poddar, Michael E. Lee, Will DeLoache, John E. Dueber, Adam P. Arkin et al. · 2014 · eLife
20. Targeted mutagenesis in soybean using the CRISPR-Cas9 system
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21. NLRP3 inflammasome activation downstream of cytoplasmic LPS recognition by both caspase‐4 and caspase‐5
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22. Optimizing sgRNA structure to improve CRISPR-Cas9 knockout efficiency
    Ying Dang, Gengxiang Jia, Jennie Choi, Hongming Ma, Edgar Anaya, Chunting Ye, Premlata Shankar, Haoquan Wu · 2015 · Genome biology
23. Gene Editing and Crop Improvement Using CRISPR-Cas9 System
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24. Saturation editing of genomic regions by multiplex homology-directed repair
    Findlay, Gregory M., Boyle, Evan A., Hause, Ronald J., Klein, Jason C., Shendure, Jay · 2014 · Nature
25. Structure and Engineering of Francisella novicida Cas9
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26. Target specificity of the CRISPR‐Cas9 system
    Xuebing Wu, Andrea J. Kriz, Phillip A. Sharp · 2014 · Quantitative Biology
27. Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping
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28. Prediction of off-target activities for the end-to-end design of CRISPR guide RNAs
    Listgarten, Jennifer, Weinstein, Michael, Kleinstiver, Benjamin P., Sousa, Alexander A., Joung, J. Keith, Crawford, Jake, Gao, Kevin, Hoang, Luong, Elibol, Melih, Doench, John G. et al. · 2018 · Nature Biomedical Engineering
29. CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons
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30. The Fanconi Anemia Pathway Maintains Genome Stability by Coordinating Replication and Transcription
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31. p62 filaments capture and present ubiquitinated cargos for autophagy
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32. Engineering of CRISPR-Cas12b for human genome editing
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33. A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection
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34. A STAT3 palmitoylation cycle promotes TH17 differentiation and colitis
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35. Making sense of GWAS: using epigenomics and genome engineering to understand the functional relevance of SNPs in non-coding regions of the human genome
    Yu Gyoung Tak, Peggy Farnham · 2015 · Epigenetics & Chromatin
36. Dynamics of CRISPR-Cas9 genome interrogation in living cells
    Spencer C. Knight, Liangqi Xie, Wulan Deng, Benjamin Guglielmi, Lea B. Witkowsky, Lana Bosanac, Elisa Zhang, Mohamed El Beheiry, Jean‐Baptiste Masson, Maxime Dahan et al. · 2015 · Science
37. Disabling Cas9 by an anti-CRISPR DNA mimic
    Jiyung J. Shin, Fuguo Jiang, Junjie Liu, Nicolas Bray, Benjamin J. Rauch, Ron Baik, Eva Nogales, Joseph Bondy‐Denomy, Jacob E. Corn, Jennifer A. Doudna · 2017 · Science Advances
38. Rapid reverse genetic screening using CRISPR in zebrafish
    Arish N Shah, Crystal F. Davey, Alex C Whitebirch, Adam C. Miller, Cecilia B. Moens · 2015 · Nature Methods
39. Kinetic Basis for DNA Target Specificity of CRISPR-Cas12a
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40. Application of genomics-assisted breeding for generation of climate resilient crops: progress and prospects
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41. WDFY4 is required for cross-presentation in response to viral and tumor antigens
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42. Characterization of Genomic Deletion Efficiency Mediated by Clustered Regularly Interspaced Palindromic Repeats (CRISPR)/Cas9 Nuclease System in Mammalian Cells*
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43. Functional Enhancers Shape Extrachromosomal Oncogene Amplifications
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44. CRISPR/Cas9-mediated targeted mutagenesis in Nicotiana tabacum
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45. CRISPR/Cas9 Based Genome Editing of Penicillium chrysogenum
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48. Precise Gene Editing Preserves Hematopoietic Stem Cell Function following Transient p53-Mediated DNA Damage Response
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49. Gene editing in plants: progress and challenges
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50. A Single Kinase Generates the Majority of the Secreted Phosphoproteome
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53. Detection of on-target and off-target mutations generated by CRISPR/Cas9 and other sequence-specific nucleases
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54. Highly Efficient Mouse Genome Editing by CRISPR Ribonucleoprotein Electroporation of Zygotes
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56. CRISPR-Based Methods for Caenorhabditis elegans Genome Engineering
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90. Targeting Hepatitis B Virus With CRISPR/Cas9
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97. CHANGE-seq reveals genetic and epigenetic effects on CRISPR–Cas9 genome-wide activity
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100. TALEN and CRISPR/Cas Genome Editing Systems: Tools of Discovery
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