High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

Pattanayak, Vikram, Lin, Steven, Guilinger, John P, Ma, Enbo, Doudna, Jennifer A, Liu, David R·2013·Nature Biotechnology

DOI 10.1038/nbt.2673

Cited by (1,002)

Items 101–200 of 1,002

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Yueping Zhang, Juan Wang, Zibai Wang, Yiming Zhang, Shuobo Shi, Jens Nielsen, Zihe Liu · 2019 · Nature Communications
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He Huang, Guosong Zheng, Weihong Jiang, Haifeng Hu, Yinhua Lü · 2015 · Acta Biochimica et Biophysica Sinica
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Pengcheng Wang, Jun Zhang, Lin Sun, Yizan Ma, Jiao Xu, Sijia Liang, Jinwu Deng, Jiafu Tan, Qinghua Zhang, Lili Tu et al. · 2017 · Plant Biotechnology Journal
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Ryan, Daniel E, Taussig, David, Steinfeld, Israel, Phadnis, Smruti M, Lunstad, Benjamin D, Singh, Madhurima, Vuong, Xuan, Okochi, Kenji D, McCaffrey, Ryan, Olesiak, Magdalena et al. · 2018 · Nucleic Acids Research
A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9
Lun Cui, A. Vigouroux, François Rousset, Hugo Varet, Varun Khanna, David Bikard · 2018 · Nature Communications
Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases
Young Hoon Sung, Jong Min Kim, Hyun-Taek Kim, Jaehoon Lee, Jisun Jeon, Young Jin, Jung-Hwa Choi, Young Ho Ban, Sang‐Jun Ha, Cheol‐Hee Kim et al. · 2013 · Genome Research
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Thorsten Langner, Sophien Kamoun, Khaoula Belhaj · 2018 · Annual Review of Phytopathology
PAM identification by CRISPR-Cas effector complexes: diversified mechanisms and structures
Daniel Gleditzsch, Patrick Pausch, Hanna Müller-Esparza, Ahsen Özcan, Xiaohan Guo, Gert Bange, Lennart Randau · 2018 · RNA Biology
Megabase-scale deletion using CRISPR/Cas9 to generate a fully haploid human cell line
Patrick Essletzbichler, Tomasz Konopka, Federica Santoro, Doris Chen, Bianca V. Gapp, Róbert Královics, Thijn R. Brummelkamp, Sebastian M. B. Nijman, Tilmann Bürckstümmer · 2014 · Genome Research
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Hongyang Du, Xuanrui Zeng, Meng Zhao, Xiaopei Cui, Qing Wang, Hui Yang, Hao Cheng, Deyue Yu · 2015 · Journal of Biotechnology
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Digvijay Singh, Samuel H. Sternberg, Jingyi Fei, Jennifer A. Doudna, Taekjip Ha · 2016 · Nature Communications
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Sharon, Eilon, Chen, Shi-An A., Khosla, Neil M., Smith, Justin D., Pritchard, Jonathan K., Fraser, Hunter B. · 2018 · Cell
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De Dong, Minghui Guo, Sihan Wang, Yuwei Zhu, Shuo Wang, Zhi Xiong, Jianzheng Yang, Zengliang Xu, Zhiwei Huang · 2017 · Nature
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Weixin Tang, David R. Liu · 2018 · Science
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Savić, Nataša, Schwank, Gerald · 2016 · Translational Research
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Ormond, Kelly E., Mortlock, Douglas P., Scholes, Derek T., Bombard, Yvonne, Brody, Lawrence C., Faucett, W. Andrew, Garrison, Nanibaa’ A., Hercher, Laura, Isasi, Rosario, Middleton, Anna et al. · 2017 · The American Journal of Human Genetics
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Tatjana I. Cornu, Claudio Mussolino, Toni Cathomen · 2017 · Nature Medicine
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Yeh, Wei-Hsi, Chiang, Hao, Rees, Holly A., Edge, Albert S. B., Liu, David R. · 2018 · Nature Communications
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Xiaoqing Zhou, Jige Xin, Nana Fan, Qingjian Zou, Jiao Huang, Zhen Ouyang, Yu Zhao, Bentian Zhao, Zhaoming Liu, Sisi Lai et al. · 2014 · Cellular and Molecular Life Sciences
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Wu, Yuxuan, Zhou, Hai, Fan, Xiaoying, Zhang, Ying, Zhang, Man, Wang, Yinghua, Xie, Zhenfei, Bai, Meizhu, Yin, Qi, Liang, Dan et al. · 2015 · Cell Research
Potential pitfalls of CRISPR /Cas9‐mediated genome editing
Rongxue Peng, Guigao Lin, Jinming Li · 2015 · FEBS Journal
Repurposing endogenous type I CRISPR-Cas systems for programmable gene repression
Michelle L. Luo, Adam S. Mullis, Ryan T. Leenay, Chase L. Beisel · 2014 · Nucleic Acids Research
Review of CRISPR/Cas9 sgRNA Design Tools
Yingbo Cui, Jiaming Xu, Minxia Cheng, Xiangke Liao, Shaoliang Peng · 2018 · Interdisciplinary Sciences Computational Life Sciences
Development of the CRISPR/Cas9 System for Targeted Gene Disruption in Aspergillus fumigatus
Fuller, Kevin K., Chen, Shan, Loros, Jennifer J., Dunlap, Jay C. · 2015 · Eukaryotic Cell
Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM
Alan S.L. Wong, Gigi C.G. Choi, Cheryl H. Cui, Gabriela Pregernig, Pamela Milani, Miriam Adam, Samuel David Perli, Samuel W. Kazer, Aleth Gaillard, Mario Hermann et al. · 2016 · Proceedings of the National Academy of Sciences
Single Cas9 nickase induced generation of NRAMP1 knockin cattle with reduced off-target effects
Yuanpeng Gao, Haibo Wu, Yongsheng Wang, Xin Liu, Linlin Chen, Qian Li, Chenchen Cui, Xu Liu, Jingcheng Zhang, Yong Zhang · 2017 · Genome biology
Generation of inheritable and “transgene clean” targeted genome-modified rice in later generations using the CRISPR/Cas9 system
Rongfang Xu, Hao Li, Ruiying Qin, Juan Li, Chun-Hong Qiu, Ya-Chun Yang, Hui Ma, Li Li, Pengcheng Wei, Jian-Bo Yang · 2015 · Scientific Reports
Cas9-chromatin binding information enables more accurate CRISPR off-target prediction
Singh, Ritambhara, Kuscu, Cem, Quinlan, Aaron, Qi, Yanjun, Adli, Mazhar · 2015 · Nucleic Acids Research
A genome-wide analysis of Cas9 binding specificity using ChIP-seq and targeted sequence capture
O'Geen, Henriette, Henry, Isabelle M., Bhakta, Mital S., Meckler, Joshua F., Segal, David J. · 2015 · Nucleic Acids Research
Efficient CRISPR-Cas9–mediated genome editing in Plasmodium falciparum
Jeffrey C. Wagner, Randall J. Platt, Stephen J. Goldfless, Feng Zhang, Jacquin C. Niles · 2014 · Nature Methods
Rapid characterization of CRISPR-Cas9 protospacer adjacent motif sequence elements
Tautvydas Karvelis, Giedrius Gasiūnas, Joshua K. Young, Greta Bigelyte, Arūnas Šilanskas, Mark Cigan, Virginijus Šikšnys · 2015 · Genome biology
CRISPR base editors: genome editing without double-stranded breaks
Ayman Eid, Sahar Abdulaziz Alshareef, Magdy M. Mahfouz · 2018 · Biochemical Journal
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Geurts, Maarten H., de Poel, Eyleen, Amatngalim, Gimano D., Oka, Rurika, Meijers, Fleur M., Kruisselbrink, Evelien, van Mourik, Peter, Berkers, Gitte, de Winter-de Groot, Karin M., Michel, Sabine et al. · 2020 · Cell Stem Cell
Biological plasticity rescues target activity in CRISPR knock outs
Smits, Arne H., Ziebell, Frederik, Joberty, Gerard, Zinn, Nico, Mueller, William F., Clauder-Münster, Sandra, Eberhard, Dirk, Fälth Savitski, Maria, Grandi, Paola, Jakob, Petra et al. · 2019 · Nature Methods
An easy and efficient inducible CRISPR/Cas9 platform with improved specificity for multiple gene targeting
Jian Ping Cao, Lizhen Wu, Shang‐Min Zhang, Min Lu, William K.C. Cheung, Wesley L. Cai, Molly Gale, Qi Xu, Qin Yan · 2016 · Nucleic Acids Research
Multigene Knockout Utilizing Off-Target Mutations of the CRISPR/Cas9 System in Rice
M. Endo, Masafumi Mikami, Seiichi Toki · 2014 · Plant and Cell Physiology
Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity
John P. Guilinger, Vikram Pattanayak, Deepak Reyon, Shengdar Q. Tsai, Jeffry D. Sander, J. Keith Joung, David R. Liu · 2014 · Nature Methods
The Functions and Unique Features of LncRNAs in Cancer Development and Tumorigenesis
Kenzui Taniue, Nobuyoshi Akimitsu · 2021 · International Journal of Molecular Sciences
Structural basis for Cas9 off-target activity
Pacesa, Martin, Lin, Chun-Han, Cléry, Antoine, Saha, Aakash, Arantes, Pablo R., Bargsten, Katja, Irby, Matthew J., Allain, Frédéric H.-T., Palermo, Giulia, Cameron, Peter et al. · 2022 · Cell
Genome-wide target specificities of CRISPR-Cas9 nucleases revealed by multiplex Digenome-seq
Kim, Daesik, Kim, Sojung, Kim, Sunghyun, Park, Jeongbin, Kim, Jin-Soo · 2016 · Genome Research
CRISPR-Cas: New Tools for Genetic Manipulations from Bacterial Immunity Systems
Wenyan Jiang, Luciano A. Marraffini · 2015 · Annual Review of Microbiology
Rapid and Scalable Characterization of CRISPR Technologies Using an E. coli Cell-Free Transcription-Translation System
Ryan Marshall, Colin S. Maxwell, Scott P. Collins, Thomas Jacobsen, Michelle L. Luo, Matthew B. Begemann, Benjamin N. Gray, Emma January, Anna Singer, Yonghua He et al. · 2018 · Molecular Cell
Measuring and Reducing Off-Target Activities of Programmable Nucleases Including CRISPR-Cas9
Taeyoung Koo, Jungjoon Lee, Jin‐Soo Kim · 2015 · Molecules and Cells
C9ORF72 repeat expansion causes vulnerability of motor neurons to Ca2+-permeable AMPA receptor-mediated excitotoxicity
Bhuvaneish Thangaraj Selvaraj, Matthew R. Livesey, Chen Zhao, Jenna M. Gregory, Owain T. James, Elaine M. Cleary, Amit K. Chouhan, Angus B. Gane, Emma Perkins, Owen Dando et al. · 2018 · Nature Communications
CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos
Yanjiao Shao, Yuting Guan, Liren Wang, Zhongwei Qiu, Meizhen Liu, Yuting Chen, Lijuan Wu, Yongmei Li, Xueyun Ma, Mingyao Liu et al. · 2014 · Nature Protocols
The CRISPR–Cas system for plant genome editing: advances and opportunities
Vinay Satish Kumar, Mukesh Jain · 2014 · Journal of Experimental Botany
High-throughput biochemical profiling reveals sequence determinants of dCas9 off-target binding and unbinding
Evan A. Boyle, Johan O. L. Andreasson, Lauren M. Chircus, Samuel H. Sternberg, Michelle J. Wu, Chantal K. Guegler, Jennifer A. Doudna, William James Greenleaf · 2017 · Proceedings of the National Academy of Sciences
Sequence features associated with the cleavage efficiency of CRISPR/Cas9 system
Xiaoxi Liu, Ayaka Homma, Jamasb Joshua Sayadi, Shu Yang, Jun Ohashi, Toru Takumi · 2016 · Scientific Reports
Efficiency and Inheritance of Targeted Mutagenesis in Maize Using CRISPR-Cas9
Jinjie Zhu, Ning Song, Silong Sun, Weilong Yang, Haiming Zhao, Weibin Song, Jinsheng Lai · 2015 · Journal of genetics and genomics/Journal of Genetics and Genomics
A machine learning approach for predicting CRISPR-Cas9 cleavage efficiencies and patterns underlying its mechanism of action
Shiran Abadi, Winston X. Yan, David Amar, Itay Mayrose · 2017 · PLoS Computational Biology
Comparison of non-canonical PAMs for CRISPR/Cas9-mediated DNA cleavage in human cells
Yilan Zhang, Xianglian Ge, Fayu Yang, Liping Zhang, Jiayong Zheng, Xuefang Tan, Zi‐Bing Jin, Jia Qu, Feng Gu · 2014 · Scientific Reports
A CRISPR toolbox to study virus–host interactions
Andreas S. Puschnik, Karim Majzoub, Yaw Shin Ooi, Jan E. Carette · 2017 · Nature Reviews Microbiology
Delivery and Specificity of CRISPR/Cas9 Genome Editing Technologies for Human Gene Therapy
Jennifer L. Gori, Patrick D. Hsu, Morgan L. Maeder, Shen Shen, G. Grant Welstead, David A. Bumcrot · 2015 · Human Gene Therapy
Incorporation of bridged nucleic acids into CRISPR RNAs improves Cas9 endonuclease specificity
Cromwell, Christopher R., Sung, Keewon, Park, Jinho, Krysler, Amanda R., Jovel, Juan, Kim, Seong Keun, Hubbard, Basil P. · 2018 · Nature Communications
TALENs facilitate targeted genome editing in human cells with high specificity and low cytotoxicity
Claudio Mussolino, Jamal Alzubi, Eli J. Fine, Robert Morbitzer, Thomas James Cradick, Thomas Lahaye, Gang Bao, Toni Cathomen · 2014 · Nucleic Acids Research
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Sanskriti Vats, Surbhi Kumawat, Virender Kumar, Gunvant Patil, Trupti Joshi, Humira Sonah, Tilak Raj Sharma, Rupesh Kailasrao Deshmukh · 2019 · Cells
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Basudeb Maji, Soumyashree A. Gangopadhyay, Miseon Lee, Meng‐Chao Shi, Peng Wu, Robert Heler, Beverly Y. Mok, Donghyun Lim, Sachini U. Siriwardena, Bishwajit Paul et al. · 2019 · Cell
Synthetic CRISPR RNA-Cas9–guided genome editing in human cells
Meghdad Rahdar, Moira A. McMahon, Thazha P. Prakash, Eric E. Swayze, C. Frank Bennett, Don W. Cleveland · 2015 · Proceedings of the National Academy of Sciences
CRISPR/Cas9 genome editing technology in filamentous fungi: progress and perspective
Runjie Song, Qing Zhai, Lu Sun, Enxia Huang, Yu Zhang, Yanli Zhu, Qingyun Guo, Yanan Tian, Baoyu Zhao, Hao Xin Lu · 2019 · Applied Microbiology and Biotechnology
Off-target predictions in CRISPR-Cas9 gene editing using deep learning
Lin, Jiecong, Wong, Ka-Chun · 2018 · Bioinformatics
Analysis of short tandem repeat expansions and their methylation state with nanopore sequencing
Pay Gießelmann, Björn Brändl, Etienne Raimondeau, Rebecca Bowen, Christian Rohrandt, Rashmi Tandon, Helene Kretzmer, Günter Assum, Christina Galonska, Reiner Siebert et al. · 2019 · Nature Biotechnology
TALE nickase-mediated SP110 knockin endows cattle with increased resistance to tuberculosis
Haibo Wu, Yongsheng Wang, Yan Zhang, Mingqi Yang, Jiaxing Lv, Jun Liu, Yong Zhang · 2015 · Proceedings of the National Academy of Sciences
Development of CRISPR-Cas systems for genome editing and beyond
F. Zhang · 2019 · Quarterly Reviews of Biophysics
Heritable genome editing with CRISPR/Cas9 induces anosmia in a crop pest moth
Fotini A. Koutroumpa, Christelle Monsempès, Marie‐Christine François, Anne De Cian, Corinne Royer, Jean‐Paul Concordet, Emmanuelle Jacquin‐Joly · 2016 · Scientific Reports
Deciphering, Communicating, and Engineering the CRISPR PAM
Ryan T. Leenay, Chase L. Beisel · 2016 · Journal of Molecular Biology
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Park, Chul-Yong, Halevy, Tomer, Lee, Dongjin R., Sung, Jin Jea, Lee, Jae Souk, Yanuka, Ofra, Benvenisty, Nissim, Kim, Dong-Wook · 2015 · Cell Reports
Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing
Carlson-Stevermer, Jared, Abdeen, Amr A., Kohlenberg, Lucille, Goedland, Madelyn, Molugu, Kaivalya, Lou, Meng, Saha, Krishanu · 2017 · Nature Communications
The Impact of Chromatin Dynamics on Cas9-Mediated Genome Editing in Human Cells
René Daer, Josh Cutts, David A. Brafman, Karmella Ann Haynes · 2016 · ACS Synthetic Biology
CRISPR-Cas9D10A nickase-based genotypic and phenotypic screening to enhance genome editing
Ting-Wei Will Chiang, Carlos le Sage, Delphine Larrieu, Mukerrem Demir, Stephen P. Jackson · 2016 · Scientific Reports
CRISPR/Cas9: A powerful tool for crop genome editing
Gaoyuan Song, Meiling Jia, Kai Chen, Xingchen Kong, Bushra Khattak, Chuanxiao Xie, Aili Li, Long Mao · 2016 · The Crop Journal