Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

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Items 501–600 of 1,030

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6. CRISPR Approaches to Small Molecule Target Identification
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13. CRISPR/Cas9: Transcending the Reality of Genome Editing
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18. CRISPR/Cas9-mediated glycolate oxidase disruption is an efficacious and safe treatment for primary hyperoxaluria type I
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19. Minimal genome-wide human CRISPR-Cas9 library
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24. Genomes in Focus: Development and Applications of CRISPR‐Cas9 Imaging Technologies
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25. Hallmarks of cancer: The CRISPR generation
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26. Improving CRISPR-Cas9 Genome Editing Efficiency by Fusion with Chromatin-Modulating Peptides
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27. Development of Improved Fruit, Vegetable, and Ornamental Crops Using the CRISPR/Cas9 Genome Editing Technique
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28. Deep learning improves the ability of sgRNA off-target propensity prediction
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29. Programmable human histone phosphorylation and gene activation using a CRISPR/Cas9-based chromatin kinase
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30. An In Vivo CRISPR Screening Platform for Prioritizing Therapeutic Targets in AML
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32. Integrated design, execution, and analysis of arrayed and pooled CRISPR genome-editing experiments
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33. GenomeCRISPR - a database for high-throughput CRISPR/Cas9 screens
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34. CRISPR/Cas9-mediated gene knockout is insensitive to target copy number but is dependent on guide RNA potency and Cas9/sgRNA threshold expression level
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35. MicroRNA-focused CRISPR-Cas9 library screen reveals fitness-associated miRNAs
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36. On-target activity predictions enable improved CRISPR–dCas9 screens in bacteria
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37. Influenza virus repurposes the antiviral protein IFIT2 to promote translation of viral mRNAs
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38. Anterior thalamic dysfunction underlies cognitive deficits in a subset of neuropsychiatric disease models
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39. Recent advances in machine learning applications in metabolic engineering
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40. Mitophagy Promotes Resistance to BH3 Mimetics in Acute Myeloid Leukemia
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41. Transcriptional and epigenetic regulators of human CD8+ T cell function identified through orthogonal CRISPR screens
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42. The key role of indole-3-acetic acid biosynthesis by Bacillus thuringiensis RZ2MS9 in promoting maize growth revealed by the ipdC gene knockout mediated by the CRISPR-Cas9 system
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44. Deep learning in CRISPR-Cas systems: a review of recent studies
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45. MARCH6 and TRC8 facilitate the quality control of cytosolic and tail‐anchored proteins
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46. Increasing consensus of context-specific metabolic models by integrating data-inferred cell functions
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47. Development and characterization of a CRISPR/Cas9n-based multiplex genome editing system for Bacillus subtilis
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48. Prediction of off-target specificity and cell-specific fitness of CRISPR-Cas System using attention boosted deep learning and network-based gene feature
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49. CRISPR Tools To Control Gene Expression in Bacteria
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50. Valine tRNA levels and availability regulate complex I assembly in leukaemia
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52. Beyond the promise: evaluating and mitigating off-target effects in CRISPR gene editing for safer therapeutics
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53. Enhanced NK-92 Cytotoxicity by CRISPR Genome Engineering Using Cas9 Ribonucleoproteins
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54. ZNF410 represses fetal globin by singular control of CHD4
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55. Targeting CCR5 as a Component of an HIV-1 Therapeutic Strategy
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57. Tandem chimeric antigen receptor (CAR) T cells targeting EGFRvIII and IL-13Rα2 are effective against heterogeneous glioblastoma
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58. New traits in crops produced by genome editing techniques based on deletions
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60. Optimization of CRISPR/Cas9 genome editing for loss-of-function in the early chick embryo
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65. Design, execution, and analysis of pooled in vitro CRISPR /Cas9 screens
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66. Toward an integrated map of genetic interactions in cancer cells
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67. Efficient combinatorial targeting of RNA transcripts in single cells with Cas13 RNA Perturb-seq
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68. INPP5D regulates inflammasome activation in human microglia
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69. CRISPRpred: A flexible and efficient tool for sgRNAs on-target activity prediction in CRISPR/Cas9 systems
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70. Inducible high-efficiency CRISPR-Cas9-targeted gene editing and precision base editing in African trypanosomes
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71. Are the current gRNA ranking prediction algorithms useful for genome editing in plants?
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72. C-RNNCrispr: Prediction of CRISPR/Cas9 sgRNA activity using convolutional and recurrent neural networks
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77. Deep learning to predict the lab-of-origin of engineered DNA
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80. A Ubiquitination Cascade Regulating the Integrated Stress Response and Survival in Carcinomas
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83. The YAP-TEAD complex promotes senescent cell survival by lowering endoplasmic reticulum stress
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84. CRISPR-Cas9 cleavage efficiency correlates strongly with target-sgRNA folding stability: from physical mechanism to off-target assessment
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85. Simultaneous Deletion of Endogenous TCRαβ for TCR Gene Therapy Creates an Improved and Safe Cellular Therapeutic
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86. gRNA validation for wheat genome editing with the CRISPR-Cas9 system
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87. A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells
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88. Highly efficient PD-1-targeted CRISPR-Cas9 for tumor-infiltrating lymphocyte-based adoptive T cell therapy
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89. Thymidine nucleotide metabolism controls human telomere length
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90. Enhancement of specialized metabolites using CRISPR/Cas gene editing technology in medicinal plants
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91. A genome-wide atlas of human cell morphology
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92. VDAC2 loss elicits tumour destruction and inflammation for cancer therapy
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93. Genetic modification of primary human B cells to model high-grade lymphoma
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94. CRISPR-mediated modeling and functional validation of candidate tumor suppressor genes in small cell lung cancer
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95. Prioritizing disease and trait causal variants at the TNFAIP3 locus using functional and genomic features
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96. Lessons from Enzyme Kinetics Reveal Specificity Principles for RNA-Guided Nucleases in RNA Interference and CRISPR-Based Genome Editing
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97. BE-FLARE: a fluorescent reporter of base editing activity reveals editing characteristics of APOBEC3A and APOBEC3B
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98. Evolution of plant mutagenesis tools: a shifting paradigm from random to targeted genome editing
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99. Keap1 mutation renders lung adenocarcinomas dependent on Slc33a1
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100. CRISPR gRNA phenotypic screening in zebrafish reveals pro-regenerative genes in spinal cord injury
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