Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

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1. Potential high-frequency off-target mutagenesis induced by CRISPR/Cas9 in Arabidopsis and its prevention
   Qiang Zhang, Hui-Li Xing, Zhi-Ping Wang, Haiyan Zhang, Fang Yang, Xuechen Wang, Qijun Chen · 2018 · Plant Molecular Biology
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   Dongrui Wang, Briana C. Prager, Ryan C. Gimple, Brenda Aguilar, Darya Alizadeh, Hongzhen Tang, Deguan Lv, Renate Starr, Alfonso Brito, Qiulian Wu et al. · 2020 · Cancer Discovery
3. A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells
   Hultquist, Judd F., Schumann, Kathrin, Woo, Jonathan M., Manganaro, Lara, McGregor, Michael J., Doudna, Jennifer, Simon, Viviana, Krogan, Nevan J., Marson, Alexander · 2016 · Cell Reports
4. Off-target predictions in CRISPR-Cas9 gene editing using deep learning
   Lin, Jiecong, Wong, Ka-Chun · 2018 · Bioinformatics
5. Precision genome editing in the CRISPR era
   Jayme Salsman, Graham Dellaire · 2016 · Biochemistry and Cell Biology
6. Marker-free coselection for CRISPR-driven genome editing in human cells
   Daniel Agudelo, Alexis Duringer, Lusiné Bozoyan, Caroline Huard, Sophie Carter, Jérémy Loehr, Dafni Synodinou, Mathieu Drouin, Jayme Salsman, Graham Dellaire et al. · 2017 · Nature Methods
7. Base editing correction of hypertrophic cardiomyopathy in human cardiomyocytes and humanized mice
   Chai, Andreas C., Cui, Miao, Chemello, Francesco, Li, Hui, Chen, Kenian, Tan, Wei, Atmanli, Ayhan, McAnally, John R., Zhang, Yu, Xu, Lin et al. · 2023 · Nature Medicine
8. Directing cellular information flow via CRISPR signal conductors
   Yuchen Liu, Yonghao Zhan, Zhicong Chen, Anbang He, Jianfa Li, Hanwei Wu, Li Liu, Chengle Zhuang, Junhao Lin, Xiaoqiang Guo et al. · 2016 · Nature Methods
9. Development of CRISPR-Cas systems for genome editing and beyond
   F. Zhang · 2019 · Quarterly Reviews of Biophysics
10. Natural variation of an EF-hand Ca2+-binding-protein coding gene confers saline-alkaline tolerance in maize
    Yibo Cao, Ming Zhang, Xiaoyan Liang, Fenrong Li, Yunlu Shi, Xiaohong Yang, Caifu Jiang · 2020 · Nature Communications
11. Insert, remove or replace: A highly advanced genome editing system using CRISPR/Cas9
    Ceasar, S. Antony, Rajan, Vinothkumar, Prykhozhij, Sergey V., Berman, Jason N., Ignacimuthu, S. · 2016 · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
12. CRISPR /Cas9‐mediated knockout of Ms1 enables the rapid generation of male‐sterile hexaploid wheat lines for use in hybrid seed production
    Anzu Okada, Taj Arndell, Nikolai V. Borisjuk, Niharika Sharma, Nathan S. Watson‐Haigh, Elise J. Tucker, Ute Baumann, Peter Langridge, Ryan Whitford · 2019 · Plant Biotechnology Journal
13. Translational Control through Differential Ribosome Pausing during Amino Acid Limitation in Mammalian Cells
    Alicia M. Darnell, Arvind Rasi Subramaniam, Erin K. O’Shea · 2018 · Molecular Cell
14. CRISPR–Cas: a tool for cancer research and therapeutics
    Hao Yin, Wen Xue, Daniel G. Anderson · 2019 · Nature Reviews Clinical Oncology
15. Machine learning meets omics: applications and perspectives
    Rufeng Li, Lixin Li, Yungang Xu, Juan Yang · 2021 · Briefings in Bioinformatics
16. CRISPR/Cas system for yeast genome engineering: advances and applications
    Vratislav Šťovíček, Carina Holkenbrink, Irina Borodina · 2017 · FEMS Yeast Research
17. The genomic landscape of TERT promoter wildtype-IDH wildtype glioblastoma
    Bill H. Diplas, Xujun He, Jacqueline A. Brosnan‐Cashman, Heng Liu, Lee H. Chen, Zhaohui Wang, Casey J. Moure, Patrick J. Killela, Daniel B. Loriaux, Eric Lipp et al. · 2018 · Nature Communications
18. Advancing genome editing with artificial intelligence: opportunities, challenges, and future directions
    Shriniket Dixit, Anant Kumar, Kathiravan Srinivasan, P. M. Durai Raj Vincent, Nadesh Ramu Krishnan · 2024 · Frontiers in Bioengineering and Biotechnology
19. Hyperactivation of HUSH complex function by Charcot–Marie–Tooth disease mutation in MORC2
    Iva A. Tchasovnikarova, Richard T. Timms, Christopher H. Douse, Rhys C. Roberts, Gordon Dougan, Robert E. Kingston, Yorgo Modis, Paul J. Lehner · 2017 · Nature Genetics
20. Generation of chromosomal deletions in dicotyledonous plants employing a user‐friendly genome editing toolkit
    Jana Ordon, Johannes Gantner, Jan Kemna, Schwalgun Lennart, Maik Reschke, Jana Streubel, Jens Boch, Johannes Stuttmann · 2016 · The Plant Journal
21. Binary pan-cancer classes with distinct vulnerabilities defined by pro- or anti-cancer YAP/TEAD activity
    Joel D. Pearson, Katherine Huang, Marek Pacal, Sean R. McCurdy, Suying Lü, Arthur Aubry, Tao Yu, Kristine M. Wadosky, Letian Zhang, Tao Wang et al. · 2021 · Cancer Cell
22. Cell surface RNAs control neutrophil recruitment
    Ningning Zhang, Wenwen Tang, Lidiane S. Torres, Xujun Wang, Yasmeen Ajaj, Li Zhu, Yi Luan, Hongyue Zhou, Yadong Wang, Dingyao Zhang et al. · 2024 · Cell
23. Cells Lacking the RB1 Tumor Suppressor Gene Are Hyperdependent on Aurora B Kinase for Survival
    Matthew G. Oser, Raquel Fonseca, Abhishek A. Chakraborty, Rachel Brough, Alexander Spektor, Rebecca B. Jennings, Abdallah Flaifel, Jesse S. S. Novak, Aditi Gulati, Elizabeth J. Buss et al. · 2018 · Cancer Discovery
24. Targeting TBK1 to overcome resistance to cancer immunotherapy
    Sun, Yi, Revach, Or-yam, Anderson, Seth, Kessler, Emily A., Wolfe, Clara H., Jenney, Anne, Mills, Caitlin E., Robitschek, Emily J., Davis, Thomas G. R., Kim, Sarah et al. · 2023 · Nature
25. Designing and executing prime editing experiments in mammalian cells
    Doman, Jordan L., Sousa, Alexander A., Randolph, Peyton B., Chen, Peter J., Liu, David R. · 2022 · Nature Protocols
26. Linear ubiquitination of cytosolic Salmonella Typhimurium activates NF-κB and restricts bacterial proliferation
    Sjoerd J. L. van Wijk, Franziska Fricke, Lina Herhaus, Jalaj Gupta, Katharina Hötte, Francesco Pampaloni, Paolo Grumati, Manuel Kaulich, Yu‐shin Sou, Masaaki Komatsu et al. · 2017 · Nature Microbiology
27. CRISPR-Cas9 off-targeting assessment with nucleic acid duplex energy parameters
    Ferhat Alkan, Anne Wenzel, Christian Anthon, Jakob H. Havgaard, Jan Gorodkin · 2018 · Genome biology
28. ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope tagging of endogenous proteins in neurons
    Jelmer Willems, Arthur P.H. de Jong, Nicky Scheefhals, Eline Jasmijn Mertens, Lisa A.E. Catsburg, Rogier B. Poorthuis, Fred de Winter, Joost Verhaagen, Frank Julius Meye, Harold D. MacGillavry · 2020 · PLoS Biology
29. R-loop formation and conformational activation mechanisms of Cas9
    Martin Pačesa, Luuk Loeff, Irma Querques, Lena M. Muckenfuss, Marta Sawicka, Martin Jínek · 2022 · Nature
30. Exposure of iPSC-derived human microglia to brain substrates enables the generation and manipulation of diverse transcriptional states in vitro
    Dolan, Michael-John, Therrien, Martine, Jereb, Saša, Kamath, Tushar, Gazestani, Vahid, Atkeson, Trevor, Marsh, Samuel E., Goeva, Aleksandrina, Lojek, Neal M., Murphy, Sarah et al. · 2023 · Nature Immunology
31. Evaluating and enhancing target specificity of gene-editing nucleases and deaminases
    Kim, Daesik, Luk, Kevin, Wolfe, Scot A., Kim, Jin-Soo · 2019 · Annual Review of Biochemistry
32. Deubiquitinases Maintain Protein Homeostasis and Survival of Cancer Cells upon Glutathione Depletion
    Isaac S. Harris, Jennifer E. Endress, Jonathan L. Coloff, Laura M. Selfors, Samuel K. McBrayer, Jennifer M. Rosenbluth, Nobuaki Takahashi, Sabin Dhakal, Vidyasagar Koduri, Matthew G. Oser et al. · 2019 · Cell Metabolism
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    Hogg, Simon J., Motorna, Olga, Cluse, Leonie A., Johanson, Timothy M., Coughlan, Hannah D., Raviram, Ramya, Myers, Robert M., Costacurta, Matteo, Todorovski, Izabela, Pijpers, Lizzy et al. · 2021 · Molecular Cell
34. Neutrophil accumulation and NET release contribute to thrombosis in HIT
    Kandace L. Gollomp, Minna Kim, Ian Johnston, Vincent Hayes, John D. Welsh, Gowthami M. Arepally, Mark L. Kahn, Michele P. Lambert, Adam C. Cuker, Douglas B. Cines et al. · 2018 · JCI Insight
35. Recording development with single cell dynamic lineage tracing
    Aaron McKenna, James A. Gagnon · 2019 · Development
36. Golgi apparatus-synthesized sulfated glycosaminoglycans mediate polymerization and activation of the cGAMP sensor STING
    Run Fang, Qifei Jiang, Yukun Guan, Pengfei Gao, Rui Zhang, Zhen Zhao, Zhengfan Jiang · 2021 · Immunity
37. The phenotypic landscape of essential human genes
    Funk, Luke, Su, Kuan-Chung, Ly, Jimmy, Feldman, David, Singh, Avtar, Moodie, Brittania, Blainey, Paul C., Cheeseman, Iain M. · 2022 · Cell
38. Protein Barcodes Enable High-Dimensional Single-Cell CRISPR Screens
    Wroblewska, Aleksandra, Dhainaut, Maxime, Ben-Zvi, Benjamin, Rose, Samuel A., Park, Eun Sook, Amir, El-Ad David, Bektesevic, Anela, Baccarini, Alessia, Merad, Miriam, Rahman, Adeeb H. et al. · 2018 · Cell
39. Multiplexed precision genome editing with trackable genomic barcodes in yeast
    Kevin R. Roy, Justin Smith, Sibylle Chantal Vonesch, Gen Lin, Chelsea Szu‐Tu, Alex R. Lederer, Angela M. Chu, Sundari Suresh, Michelle Nguyen, Joe Horecka et al. · 2018 · Nature Biotechnology
40. A CRISPR/Cas9-Engineered ARID1A -Deficient Human Gastric Cancer Organoid Model Reveals Essential and Nonessential Modes of Oncogenic Transformation
    Lo, Yuan-Hung, Kolahi, Kevin S., Du, Yuhong, Chang, Chiung-Ying, Krokhotin, Andrey, Nair, Ajay, Sobba, Walter D., Karlsson, Kasper, Jones, Sunny J., Longacre, Teri A. et al. · 2021 · Cancer Discovery
41. Functional interrogation of a SARS-CoV-2 host protein interactome identifies unique and shared coronavirus host factors
    Hans-Heinrich Hoffmann, Francisco J. Sánchez Rivera, William M. Schneider, Joseph M. Luna, Yadira M. Soto‐Feliciano, Alison W. Ashbrook, Jérémie Le Pen, Andrew A. Leal, Inna Ricardo-Lax, Eleftherios Michailidis et al. · 2020 · Cell Host & Microbe
42. SCORPIUS improves trajectory inference and identifies novel modules in dendritic cell development
    Robrecht Cannoodt, Wouter Saelens, Dorine Sichien, Simon Jan Tavernier, Sophie Janssens, Martin Guilliams, Bart N. Lambrecht, Katleen De Preter, Yvan Saeys · 2016 · bioRxiv (Cold Spring Harbor Laboratory)
43. Titrating gene expression using libraries of systematically attenuated CRISPR guide RNAs
    Jost, Marco, Santos, Daniel A., Saunders, Reuben A., Horlbeck, Max A., Hawkins, John S., Scaria, Sonia M., Norman, Thomas M., Hussmann, Jeffrey A., Liem, Christina R., Gross, Carol A. et al. · 2020 · Nature Biotechnology
44. Dnmt3a -mutated clonal hematopoiesis promotes osteoporosis
    Kim, Peter Geon, Niroula, Abhishek, Shkolnik, Veronica, McConkey, Marie, Lin, Amy E., Słabicki, Mikołaj, Kemp, John P., Bick, Alexander, Gibson, Christopher J., Griffin, Gabriel et al. · 2021 · Journal of Experimental Medicine
45. METTL16 drives leukemogenesis and leukemia stem cell self-renewal by reprogramming BCAA metabolism
    Li Han, Lei Dong, Keith Leung, Zhicong Zhao, Yang-Chan Li, Lei Gao, Zhen-Hua Chen, Jingling Xue, Ying Qing, Wei Li et al. · 2023 · Cell Stem Cell
46. KEAP1 loss modulates sensitivity to kinase targeted therapy in lung cancer
    Krall, Elsa B, Wang, Belinda, Munoz, Diana M, Ilic, Nina, Raghavan, Srivatsan, Niederst, Matthew J, Yu, Kristine, Ruddy, David A, Aguirre, Andrew J, Kim, Jong Wook et al. · 2017 · eLife
47. Machine Learning and Deep Learning in Synthetic Biology: Key Architectures, Applications, and Challenges
    Manoj Kumar Goshisht · 2024 · ACS Omega
48. CRISPR/Cas9-Mediated Scanning for Regulatory Elements Required for HPRT1 Expression via Thousands of Large, Programmed Genomic Deletions
    Gasperini, Molly, Findlay, Gregory M., McKenna, Aaron, Milbank, Jennifer H., Lee, Choli, Zhang, Melissa D., Cusanovich, Darren A., Shendure, Jay · 2017 · The American Journal of Human Genetics
49. Simultaneous repression of multiple bacterial genes using nonrepetitive extra-long sgRNA arrays
    Alexander C. Reis, Sean M. Halper, Grace E. Vezeau, Daniel P. Cetnar, Ayaan Hossain, Phillip R. Clauer, Howard M. Salis · 2019 · Nature Biotechnology
50. Exploring the potential of genome editing CRISPR-Cas9 technology
    Vijai Singh, Darren Braddick, Pawan Kumar Dhar · 2016 · Gene
51. Size-Dependent Segregation Controls Macrophage Phagocytosis of Antibody-Opsonized Targets
    Matthew H. Bakalar, Aaron M. Joffe, Eva M. Schmid, Sungmin Son, Marija Podolski, Daniel A. Fletcher · 2018 · Cell
52. Three million images and morphological profiles of cells treated with matched chemical and genetic perturbations
    Srinivas Niranj Chandrasekaran, Beth A. Cimini, Amy B. Goodale, Lisa Miller, Maria Kost‐Alimova, Nasim Jamali, John G. Doench, Briana Fritchman, Adam P. Skepner, Michelle Melanson et al. · 2024 · Nature Methods
53. CRISPR/Cas9-mediated mutagenesis of the dihydroflavonol-4-reductase-B (DFR-B) locus in the Japanese morning glory Ipomoea (Pharbitis) nil
    Kenta Watanabe, Anna Kobayashi, M. Endo, Kimiyo Sage‐Ono, Seiichi Toki, Michiyuki Ono · 2017 · Scientific Reports
54. Cleave and Rescue, a novel selfish genetic element and general strategy for gene drive
    Georg Oberhofer, Tobin Ivy, Bruce A. Hay · 2019 · Proceedings of the National Academy of Sciences
55. Multi-phenotype CRISPR-Cas9 Screen Identifies p38 Kinase as a Target for Adoptive Immunotherapies
    Devikala Gurusamy, Amanda N. Henning, Tori N. Yamamoto, Zhiya Yu, Nikolaos Zacharakis, Sri Krishna, Rigel J. Kishton, Suman Kumar Vodnala, Arash Eidizadeh, Li Jia et al. · 2020 · Cancer Cell
56. A web tool for the design of prime-editing guide RNAs
    Ryan Dz-Wei Chow, Jennifer Chen, Johanna Shen, Sidi Chen · 2020 · Nature Biomedical Engineering
57. Radiotherapy orchestrates natural killer cell dependent antitumor immune responses through CXCL8
    Thomas Walle, Joscha A. Kraske, Boyu Liao, Bénédicte Lenoir, Carmen Timke, Emilia von Bohlen und Halbach, Florian Tran, Paul Griebel, Dorothee Albrecht, Azaz Ahmed et al. · 2022 · Science Advances
58. Derivation and differentiation of haploid human embryonic stem cells
    Ido Sagi, Gloryn Chia, Tamar Golan‐Lev, Mordecai Peretz, Uri Weissbein, Lina Sui, Mark V. Sauer, Ofra Yanuka, Dieter M. Egli, Nissim Benvenisty · 2016 · Nature
59. Advances in Engineering the Fly Genome with the CRISPR-Cas System
    Ethan Bier, Melissa M. Harrison, Kate M. O’Connor-Giles, Jill Wildonger · 2018 · Genetics
60. The midnolin-proteasome pathway catches proteins for ubiquitination-independent degradation
    Xin Gu, Christopher Nardone, Nolan Kamitaki, Aoyue Mao, Stephen Joseph Elledge, Michael E. Greenberg · 2023 · Science
61. Multilayered VBC score predicts sgRNAs that efficiently generate loss-of-function alleles
    Michlits, Georg, Jude, Julian, Hinterndorfer, Matthias, de Almeida, Melanie, Vainorius, Gintautas, Hubmann, Maria, Neumann, Tobias, Schleiffer, Alexander, Burkard, Thomas Rainer, Fellner, Michaela et al. · 2020 · Nature Methods
62. Enhancer Architecture and Essential Core Regulatory Circuitry of Chronic Lymphocytic Leukemia
    Christopher J. Ott, Alexander Joel Federation, Logan Sari Schwartz, Siddha N. Kasar, Josephine L. Klitgaard, Romina Lenci, Qiyuan Li, Matthew A. Lawlor, Stacey M. Fernandes, Amanda Souza et al. · 2018 · Cancer Cell
63. Glycosidase and glycan polymorphism control hydrolytic release of immunogenic flagellin peptides
    Pierre Buscaill, Balakumaran Chandrasekar, Nattapong Sanguankiattichai, Jiorgos Kourelis, Farnusch Kaschani, Emma Thomas, Kyoko Morimoto, Markus Kaiser, Gail M. Preston, Yuki Ichinose et al. · 2019 · Science
64. Paralog knockout profiling identifies DUSP4 and DUSP6 as a digenic dependence in MAPK pathway-driven cancers
    Takahiro Ito, Michael J. Young, Ruitong Li, Sidharth S Jain, Andreas Wernitznig, John Michael Krill-Burger, Christopher T. Lemke, Davide Monducci, Diego J. Rodriguez, Liang Chang et al. · 2021 · Nature Genetics
65. A first-generation pediatric cancer dependency map
    Dharia, Neekesh V., Kugener, Guillaume, Guenther, Lillian M., Malone, Clare F., Durbin, Adam D., Hong, Andrew L., Howard, Thomas P., Bandopadhayay, Pratiti, Wechsler, Caroline S., Fung, Iris et al. · 2021 · Nature Genetics
66. CRISPR-Cas9 screen reveals a MYCN-amplified neuroblastoma dependency on EZH2
    Liying Chen, Gabriela Alexe, Neekesh V. Dharia, Linda S. Ross, Amanda Balboni Iniguez, Amy Saur Conway, Emily Jue Wang, Veronica Veschi, Norris Lam, Jun Qi et al. · 2017 · Journal of Clinical Investigation
67. A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV
    Molly OhAinle, Louisa Helms, Jolien Vermeíre, Ferdinand Roesch, Daryl G. Humes, Ryan Basom, Jeffrey J. Delrow, Julie M. Overbaugh, Michael Emerman · 2018 · eLife
68. TCR signal strength defines distinct mechanisms of T cell dysfunction and cancer evasion
    Mojdeh Shakiba, Paul Zumbo, Gabriel Espinosa-Carrasco, Laura Menocal, Friederike Dündar, Sandra E. Carson, Emmanuel M. Bruno, Francisco J. Sánchez Rivera, Scott W. Lowe, Steven Camara et al. · 2021 · The Journal of Experimental Medicine
69. The proteogenomic subtypes of acute myeloid leukemia
    Ashok Kumar Jayavelu, Sebastian Wolf, Florian Buettner, Gabriela Alexe, Björn Häupl, Federico Comoglio, Constanze Schneider, Carmen Doebele, Dominik Christian Fuhrmann, Sebastian Wagner et al. · 2022 · Cancer Cell
70. FlashFry: A fast and flexible tool for large-scale CRISPR target design
    McKenna, Aaron, Shendure, Jay · 2018 · BMC Biology
71. What are housekeeping genes?
    Chintan Jagdishchandra Joshi, Wenfan Ke, Anna Drangowska-Way, Eyleen J. O’Rourke, Nathan E. Lewis · 2022 · PLoS Computational Biology
72. Cholesterol accessibility at the ciliary membrane controls hedgehog signaling
    Maia Kinnebrew, Ellen J. Iverson, Bhaven B. Patel, Ganesh Varma Pusapati, Jennifer H. Kong, Kristen A. Johnson, Giovanni Luchetti, Kaitlyn M. Eckert, Jeffrey G. McDonald, Douglas F. Covey et al. · 2019 · eLife
73. Engineered CRISPR-Cas systems for the detection and control of antibiotic-resistant infections
    Yuye Wu, Dheerendranath Battalapalli, Mohammed J. Hakeem, Venkatarao Selamneni, Pengfei Zhang, Mohamed Shehata Draz, Zhi Ruan · 2021 · Journal of Nanobiotechnology
74. Defective ALC1 nucleosome remodeling confers PARPi sensitization and synthetic lethality with HRD
    Graeme Hewitt, Valérie Borel, Sandra Segura‐Bayona, Tohru Takaki, Phil Ruis, Roberto Bellelli, Laura C. Lehmann, Lucia Sommerová, Aleksandra Vančevska, Antonia Tomás‐Loba et al. · 2020 · Molecular Cell
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    Nicolaas J. Boon, Rafaela A. Oliveira, Pierre-René Körner, Adva Kochavi, Sander Mertens, Yuval Malka, Rhianne Voogd, Suzanne E. M. van der Horst, Maarten A. Huismans, Lidwien P. Smabers et al. · 2024 · Science
76. A Compendium of Genetic Modifiers of Mitochondrial Dysfunction Reveals Intra-organelle Buffering
    Tsz‐Leung To, Alejandro M. Cuadros, Hardik Shah, Wendy H.W. Hung, Li Yang, Sharon H. Kim, Daniel H. F. Rubin, Ryan H. Boe, Sneha P. Rath, John K. Eaton et al. · 2019 · Cell
77. QRICH1 dictates the outcome of ER stress through transcriptional control of proteostasis
    Kwontae You, Lingfei Wang, Chih‐Hung Chou, Kai Liu, Toru Nakata, Alok Jaiswal, Junmei Yao, Ariel J. Lefkovith, Abdifatah S. Omar, Jacqueline Perrigoue et al. · 2020 · Science
78. MHC class II proteins mediate cross-species entry of bat influenza viruses
    Umut Karakus, Thiprampai Thamamongood, Kevin Ciminski, Wei Ran, Sira Carolin Günther, Marie O. Pohl, Davide Eletto, Csaba Jeney, Donata Hoffmann, Sven Reiche et al. · 2019 · Nature
79. Mitigation of off-target toxicity in CRISPR-Cas9 screens for essential non-coding elements
    Josh Tycko, Michael Wainberg, Georgi K. Marinov, Oana Ursu, Gaelen T. Hess, Braeden K. Ego, Aradhana, Amy Li, Alisa Truong, Alexandro E. Trevino et al. · 2019 · Nature Communications
80. Template-assisted covalent modification underlies activity of covalent molecular glues
    Yen-Der Li, Michelle W. Ma, Muhammad Murtaza Hassan, Moritz Hunkeler, Mingxing Teng, Kedar Puvar, Justine C. Rutter, Ryan J. Lumpkin, Brittany Sandoval, Cyrus Y. Jin et al. · 2024 · Nature Chemical Biology
81. Cystine uptake through the cystine/glutamate antiporter xCT triggers glioblastoma cell death under glucose deprivation
    Takeo Goji, Kazuhiko Takahara, Masahiko Negishi, Hironori Katoh · 2017 · Journal of Biological Chemistry
82. R-spondins can potentiate WNT signaling without LGRs
    Andres M. Lebensohn, Rajat Rohatgi · 2018 · eLife
83. Applications of the CRISPR/Cas9 System for Rice Grain Quality Improvement: Perspectives and Opportunities
    Sajid Fiaz, Shakeel Ahmad, Mehmood Ali Noor, Xiukang Wang, Afifa Younas, Aamir Riaz, Adeel Riaz, Fahad Ali · 2019 · International Journal of Molecular Sciences
84. CRISPR-GPT for agentic automation of gene-editing experiments
    Yuanhao Qu, Kaixuan Huang, Ming Yin, Kanghong Zhan, Dyllan Liu, Di Yin, Henry C. Cousins, William A. Johnson, Xiaotong Wang, Mihir M. Shah et al. · 2025 · Nature Biomedical Engineering
85. CRISPR screening using an expanded toolkit of autophagy reporters identifies TMEM41B as a novel autophagy factor
    Christopher J. Shoemaker, Tina Qing Huang, Nicholas R. Weir, Nicole J. Polyakov, Sebastian Wolfgang Schultz, Vladimir Denic · 2019 · PLoS Biology
86. Comprehensive Molecular Characterization Identifies Distinct Genomic and Immune Hallmarks of Renal Medullary Carcinoma
    Pavlos Msaouel, Gabriel G. Malouf, Xiaoping Su, Hui Yao, Durga Nand Tripathi, Melinda Soeung, Jianjun Gao, Priya Rao, Cristian Coarfa, Chad J. Creighton et al. · 2019 · Cancer Cell
87. Genome-wide Screens Identify Lineage- and Tumor-Specific Genes Modulating MHC-I- and MHC-II-Restricted Immunosurveillance of Human Lymphomas
    Devin Dersh, James D. Phelan, Megan E. Gumina, Boya Wang, Jesse H. Arbuckle, Jaroslav Hollý, Rigel J. Kishton, Tovah E. Markowitz, Mina O. Seedhom, Nathan Fridlyand et al. · 2020 · Immunity
88. Blockade or Deletion of IFNγ Reduces Macrophage Activation without Compromising CAR T-cell Function in Hematologic Malignancies
    Stefanie R. Bailey, Sonika Vatsa, Rebecca C. Larson, Amanda A. Bouffard, Irene Scarfò, Michael C. Kann, Trisha R. Berger, Mark B. Leick, Marc Wehrli, Andrea Schmidts et al. · 2021 · Blood Cancer Discovery
89. ESCRT-dependent STING degradation inhibits steady-state and cGAMP-induced signalling
    Matteo Gentili, Bingxu Liu, Malvina Papanastasiou, Deborah O. Dele-Oni, Marc A. Schwartz, Rebecca J. Carlson, Aziz M. Al’Khafaji, Karsten Krug, Adam Brown, John G. Doench et al. · 2023 · Nature Communications
90. Autoregulatory control of mitochondrial glutathione homeostasis
    Yuyang Liu, Shanshan Liu, Anju Tomar, Frederick S. Yen, Gökhan Ünlü, Nathalie Ropek, Ross A. Weber, Ying Wang, Artem Khan, Mark Gad et al. · 2023 · Science
91. High-throughput analysis of the activities of xCas9, SpCas9-NG and SpCas9 at matched and mismatched target sequences in human cells
    Kim, Hui Kwon, Lee, Sungtae, Kim, Younggwang, Park, Jinman, Min, Seonwoo, Choi, Jae Woo, Huang, Tony P., Yoon, Sungroh, Liu, David R., Kim, Hyongbum Henry · 2020 · Nature Biomedical Engineering
92. CRISPR-Cas9 for in vivo Gene Therapy: Promise and Hurdles
    Wei-Jing Dai, Liyao Zhu, Zhongyi Yan, Yong Xu, Qilong Wang, Xiaojie Lu · 2016 · Molecular Therapy — Nucleic Acids
93. Targeted mutagenesis in wheat microspores using CRISPR/Cas9
    Pankaj Bhowmik, Evan E. Ellison, Brittany Polley, Venkatesh Bollina, Manoj M Kulkarni, Kaveh Ghanbarnia, Halim Song, Caixia Gao, Daniel F. Voytas, Sateesh Kagale · 2018 · Scientific Reports
94. Predicting cell health phenotypes using image-based morphology profiling
    Gregory P. Way, Maria Kost‐Alimova, Tsukasa Shibue, William F. Harrington, Stanley Gill, Federica Piccioni, Tim Becker, Hamdah Shafqat-Abbasi, William C. Hahn, Anne Elizabeth Carpenter et al. · 2021 · Molecular Biology of the Cell
95. Genetic interaction mapping and exon-resolution functional genomics with a hybrid Cas9–Cas12a platform
    Gonatopoulos-Pournatzis, Thomas, Aregger, Michael, Brown, Kevin R., Farhangmehr, Shaghayegh, Braunschweig, Ulrich, Ward, Henry N., Ha, Kevin C. H., Weiss, Alexander, Billmann, Maximilian, Durbic, Tanja et al. · 2020 · Nature Biotechnology
96. CRISPR/Cas9-mediated editing of CsWRKY22 reduces susceptibility to Xanthomonas citri subsp. citri in Wanjincheng orange (Citrus sinensis (L.) Osbeck)
    Lijuan Wang, Shanchun Chen, Aihong Peng, Zhu Xie, Yongrui He, Xiuping Zou · 2019 · Plant Biotechnology Reports
97. Natural Killer Cells Suppress T Cell-Associated Tumor Immune Evasion
    Andrew J. Freeman, Stephin J. Vervoort, Kelly M. Ramsbottom, Madison J. Kelly, Jessica Michie, Lizzy Pijpers, Ricky W. Johnstone, Conor J. Kearney, Jane Oliaro · 2019 · Cell Reports
98. Mitochondrial cristae architecture protects against mtDNA release and inflammation
    Baiyu He, Huatong Yu, Shanshan Liu, Huayun Wan, Song Fu, Siqi Liu, Jun Jie Yang, Zihan Zhang, Huanwei Huang, Qi Li et al. · 2022 · Cell Reports
99. High-Resolution Analysis of the Efficiency, Heritability, and Editing Outcomes of CRISPR/Cas9-Induced Modifications of NCED4 in Lettuce ( Lactuca sativa )
    Lien D. Bertier, Mily Ron, Heqiang Huo, Kent J. Bradford, Anne Britt, Richard W. Michelmore · 2018 · G3 Genes Genomes Genetics
100. Identification of DHODH as a therapeutic target in small cell lung cancer
     Leanne Li, Sheng Rong Ng, Caterina I. Colón, Benjamin Jacob Drapkin, Peggy P. Hsu, Zhaoqi Li, Christopher S. Nabel, Caroline A. Lewis, Rodrigo Romero, Kim L. Mercer et al. · 2019 · Science Translational Medicine