Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

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2. Base editing rescue of spinal muscular atrophy in cells and in mice
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3. CRISPR-UMI: single-cell lineage tracing of pooled CRISPR–Cas9 screens
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4. High‐efficiency genome editing using a dmc1 promoter‐controlled CRISPR /Cas9 system in maize
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5. Modeling Human Digestive Diseases With CRISPR-Cas9–Modified Organoids
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6. Role of artificial intelligence in revolutionizing drug discovery
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7. Simultaneous CRISPR screening and spatial transcriptomics reveal intracellular, intercellular, and functional transcriptional circuits
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8. Genome-wide CRISPR-Cas9 Screen Identifies Leukemia-Specific Dependence on a Pre-mRNA Metabolic Pathway Regulated by DCPS
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9. DNA stretching induces Cas9 off-target activity
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11. CRISPR/Cas9 Screens Reveal Epstein-Barr Virus-Transformed B Cell Host Dependency Factors
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13. Cell fitness screens reveal a conflict between LINE-1 retrotransposition and DNA replication
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14. Computational and experimental performance of CRISPR homing gene drive strategies with multiplexed gRNAs
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15. Mitigating off-target effects in CRISPR/Cas9-mediated in vivo gene editing
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16. Generation and validation of homozygous fluorescent knock-in cells using CRISPR-Cas9 genome editing
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17. RIG-I activation is critical for responsiveness to checkpoint blockade
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18. Biosystems Design by Machine Learning
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19. Activating PTEN Tumor Suppressor Expression with the CRISPR/dCas9 System
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20. Massively parallel kinetic profiling of natural and engineered CRISPR nucleases
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21. Promoting Cas9 degradation reduces mosaic mutations in non-human primate embryos
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22. The CRISPR/Cas revolution continues: From efficient gene editing for crop breeding to plant synthetic biology
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23. Engineered Extracellular Vesicle-Delivered CRISPR/Cas9 for Radiotherapy Sensitization of Glioblastoma
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24. Suppression of Adaptive Responses to Targeted Cancer Therapy by Transcriptional Repression
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25. VPS37A directs ESCRT recruitment for phagophore closure
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26. Targeting advanced prostate cancer with STEAP1 chimeric antigen receptor T cell and tumor-localized IL-12 immunotherapy
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27. Donafenib and GSK‐J4 Synergistically Induce Ferroptosis in Liver Cancer by Upregulating HMOX1 Expression
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28. Genome-scale screens identify JNK–JUN signaling as a barrier for pluripotency exit and endoderm differentiation
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29. Functional regulatory variants implicate distinct transcriptional networks in dementia
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30. Exosomal PD‐L1 functions as an immunosuppressant to promote wound healing
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31. Last step in the path of LDL cholesterol from lysosome to plasma membrane to ER is governed by phosphatidylserine
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32. Versatile detection with CRISPR/Cas system from applications to challenges
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33. Computational Tools and Resources for CRISPR/Cas Genome Editing
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34. ATXN1L, CIC, and ETS Transcription Factors Modulate Sensitivity to MAPK Pathway Inhibition
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35. Genome-wide specificity of prime editors in plants
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36. Manipulating mitochondrial electron flow enhances tumor immunogenicity
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37. In Silico Meets In Vivo : Towards Computational CRISPR-Based sgRNA Design
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38. Rapid generation of genetic diversity by multiplex CRISPR/Cas9 genome editing in rice
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39. Somatic Superenhancer Duplications and Hotspot Mutations Lead to Oncogenic Activation of the KLF5 Transcription Factor
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40. CRISPR–Cas9 genome engineering of primary CD4+ T cells for the interrogation of HIV–host factor interactions
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41. STAG2 Loss Rewires Oncogenic and Developmental Programs to Promote Metastasis in Ewing Sarcoma
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42. Efficient Inactivation of Symbiotic Nitrogen Fixation Related Genes in Lotus japonicus Using CRISPR-Cas9
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43. CRISPR Screens Uncover Genes that Regulate Target Cell Sensitivity to the Morphogen Sonic Hedgehog
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44. Functional analysis of the TM6 MADS-box gene in the octoploid strawberry by CRISPR/Cas9-directed mutagenesis
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47. Optimizing the CRISPR/Cas9 system for genome editing in grape by using grape promoters
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48. Genome-wide screen identifies cullin-RING ligase machinery required for lenalidomide-dependent CRL4CRBN activity
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49. The Current State and Future of CRISPR-Cas9 gRNA Design Tools
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50. Human genetic diversity alters off-target outcomes of therapeutic gene editing
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51. Functional Genomics Identify Distinct and Overlapping Genes Mediating Resistance to Different Classes of Heterobifunctional Degraders of Oncoproteins
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52. Engineering cell signaling using tunable CRISPR–Cpf1-based transcription factors
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53. Base editing sensor libraries for high-throughput engineering and functional analysis of cancer-associated single nucleotide variants
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54. A Genome-wide CRISPR Screen Reveals a Role for the Non-canonical Nucleosome-Remodeling BAF Complex in Foxp3 Expression and Regulatory T Cell Function
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55. Imaging-based pooled CRISPR screening reveals regulators of lncRNA localization
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56. CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells
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57. Applications of CRISPR-Cas Enzymes in Cancer Therapeutics and Detection
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58. Unsupervised correction of gene-independent cell responses to CRISPR-Cas9 targeting
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59. Genome-wide CRISPR-Cas9 Interrogation of Splicing Networks Reveals a Mechanism for Recognition of Autism-Misregulated Neuronal Microexons
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61. Droplet-based forward genetic screening of astrocyte–microglia cross-talk
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62. Ovarian cancer-derived IL-4 promotes immunotherapy resistance
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63. Plasticity of the Cullin-RING Ligase Repertoire Shapes Sensitivity to Ligand-Induced Protein Degradation
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64. Cancer CRISPR Screens In Vivo
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65. MYC Controls the Epstein-Barr Virus Lytic Switch
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66. A versatile CRISPR-Cas13d platform for multiplexed transcriptomic regulation and metabolic engineering in primary human T cells
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67. sgRNA Sequence Motifs Blocking Efficient CRISPR/Cas9-Mediated Gene Editing
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68. The hyaluronidase, TMEM2, promotes ER homeostasis and longevity independent of the UPRER
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70. Brainwide silencing of prion protein by AAV-mediated delivery of an engineered compact epigenetic editor
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71. Genome-Wide CRISPR Screen for Essential Cell Growth Mediators in Mutant KRAS Colorectal Cancers
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72. A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system
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74. In vivo CRISPR/Cas9 targeting of fusion oncogenes for selective elimination of cancer cells
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76. sgRNACNN: identifying sgRNA on-target activity in four crops using ensembles of convolutional neural networks
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77. Natural variation in gene expression and viral susceptibility revealed by neural progenitor cell villages
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78. Hybridization Kinetics Explains CRISPR-Cas Off-Targeting Rules
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79. Targeted Deletion of the USTA and UvSLT2 Genes Efficiently in Ustilaginoidea virens With the CRISPR-Cas9 System
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80. Salvage of ribose from uridine or RNA supports glycolysis in nutrient-limited conditions
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81. Complementary information derived from CRISPR Cas9 mediated gene deletion and suppression
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82. Zinc Fingers, TALEs, and CRISPR Systems: A Comparison of Tools for Epigenome Editing
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83. Targetable vulnerabilities in T- and NK-cell lymphomas identified through preclinical models
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85. Tracking Calcium Dynamics and Immune Surveillance at the Choroid Plexus Blood-Cerebrospinal Fluid Interface
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86. TRIM8 modulates the EWS/FLI oncoprotein to promote survival in Ewing sarcoma
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88. Human genetic variation alters CRISPR-Cas9 on- and off-targeting specificity at therapeutically implicated loci
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89. Reduced mitochondrial fusion and Huntingtin levels contribute to impaired dendritic maturation and behavioral deficits in Fmr1-mutant mice
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90. Receptor‐mediated clustering of FIP200 bypasses the role of LC3 lipidation in autophagy
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91. Behavior of homing endonuclease gene drives targeting genes required for viability or female fertility with multiplexed guide RNAs
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92. CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity
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93. Targeted inhibition of tumor-derived exosomes as a novel therapeutic option for cancer
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94. Using traditional machine learning and deep learning methods for on- and off-target prediction in CRISPR/Cas9: a review
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95. Selective degradation of multimeric proteins by TRIM21-based molecular glue and PROTAC degraders
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97. PTPN14 degradation by high-risk human papillomavirus E7 limits keratinocyte differentiation and contributes to HPV-mediated oncogenesis
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98. Prediction of CRISPR sgRNA Activity Using a Deep Convolutional Neural Network
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99. Myc and Dnmt1 impede the pluripotent to totipotent state transition in embryonic stem cells
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100. Validating genome-wide CRISPR-Cas9 function improves screening in the oleaginous yeast Yarrowia lipolytica
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