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In vivo tumor targeting and spectroscopic detection with surface-enhanced Raman nanoparticle tags
Abstract
We describe biocompatible and nontoxic nanoparticles for in vivo tumor targeting and detection based on pegylated gold nanoparticles and surface-enhanced Raman scattering (SERS). Colloidal gold has been safely used to treat rheumatoid arthritis for 50 years, and has recently been found to amplify the efficiency of Raman scattering by 14-15 orders of magnitude. Here we show that large optical enhancements can be achieved under in vivo conditions for tumor detection in live animals. An important finding is that small-molecule Raman reporters such as organic dyes were not displaced but were stabilized by thiol-modified polyethylene glycols. These pegylated SERS nanoparticles were considerably brighter than semiconductor quantum dots with light emission in the near-infrared window. When conjugated to tumor-targeting ligands such as single-chain variable fragment (ScFv) antibodies, the conjugated nanoparticles were able to target tumor biomarkers such as epidermal growth factor receptors on human cancer cells and in xenograft tumor models.
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“For example, Raman spectroscopy, a laboratory technique used to observe vibrational, rotational, and other low-frequency changes in molecules of a system, is now being used for in vivo detection of tumors (Qian et al., 2008).”
From The Astrobiology Primer v2.0 (2016) · cites this paper as Qian et al., 2008
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Metadata
- Authors
- Qian, X., Peng, X.-H., Ansari, D. O., Yin-Goen, Q., Chen, G. Z., Shin, D. M., Yang, L., Young, A. N., Wang, M. D., & Nie, S.
- Affiliations
- Georgia Institute of Technology (US)
- Emory University (US)
- Corresponding author
- Nie, Shuming
- Venue
- Nature Biotechnology
- Published
- 2007-12-23
- Volume
- 26
- Issue
- 1
- Pages
- 83-90
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- OpenAlex
- W4234368842
- Cited by
- 2,201
- References
- 55
- Citations by year
- 2026: 38 · 2025: 43 · 2024: 62 · 2023: 57 · 2022: 74 · 2021: 82 · 2020: 100 · 2019: 116 · 2018: 121 · 2017: 163 · 2016: 149 · 2015: 183 · 2014: 199 · 2013: 165 · 2012: 166
- FWCI
- 51.55
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute (R01CA108468, U54CA119338, P50CA128613)
- National Institute of General Medical Sciences (T32GM008169)
- Topics
- Physical Sciences › Materials Science › Electronic, Optical and Magnetic Materials › Gold and Silver Nanoparticles Synthesis and Applications
- Physical Sciences › Engineering › Biomedical Engineering › Nanoplatforms for cancer theranostics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Spectroscopy Techniques in Biomedical and Chemical Research
- Keywords
- surface-enhanced Raman scattering, gold nanoparticles, PEGylated nanoparticles, Raman imaging, scFv, epidermal growth factor receptor, tumor xenografts, cancer detection, biocompatible nanoparticles
- MeSH terms
- Animals, Carcinoma, Squamous Cell, Humans, Particle Size, Spectrum Analysis, Raman, Drug Delivery Systems, Cell Line, Tumor, Mice, Nanoparticles
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