Functionally distinct kinesin-13 family members cooperate to regulate microtubule dynamics during interphase
Abstract
Regulation of microtubule polymerization and depolymerization is required for proper cell development. Here, we report that two proteins of the Drosophila melanogaster kinesin-13 family, KLP10A and KLP59C, cooperate to drive microtubule depolymerization in interphase cells. Analyses of microtubule dynamics in S2 cells depleted of these proteins indicate that both proteins stimulate depolymerization, but alter distinct parameters of dynamic instability; KLP10A stimulates catastrophe (a switch from growth to shrinkage) whereas KLP59C suppresses rescue (a switch from shrinkage to growth). Moreover, immunofluorescence and live analyses of cells expressing tagged kinesins reveal that KLP10A and KLP59C target to polymerizing and depolymerizing microtubule plus ends, respectively. Our data also suggest that KLP10A is deposited on microtubules by the plus-end tracking protein, EB1. Our findings support a model in which these two members of the kinesin-13 family divide the labour of microtubule depolymerization.
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MCAK plus-end tracking requires interaction between MCAK and members of the end-binding protein (EB) family.
“This plus-end tracking behavior requires the interaction between MCAK and members of the end-binding protein (EB) family [5-8]”
From In vitro reconstitution of the functional interplay between MCAK and EB3 at microtubule plus ends (2010) · cites this paper as [8]
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- Authors
- Mennella, V., Rogers, G. C., Rogers, S. L., Buster, D. W., Vale, R. D., & Sharp, D. J.
- Affiliations
- Albert Einstein College of Medicine (US)
- Howard Hughes Medical Institute (US)
- Corresponding author
- Sharp, David J.
- Venue
- Nature Cell Biology
- Published
- 2005-02-20
- Volume
- 7
- Issue
- 3
- Pages
- 235-245
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W2134553114
- Cited by
- 154
- References
- 47
- Citations by year
- 2026: 1 · 2025: 3 · 2024: 4 · 2023: 4 · 2022: 13 · 2021: 2 · 2020: 5 · 2019: 3 · 2018: 2 · 2017: 2 · 2016: 8 · 2015: 6 · 2014: 7 · 2013: 8 · 2012: 13
- FWCI
- 5.29
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- ePrints Soton (University of Southampton) (submittedVersion)
- Funders
- Leukemia and Lymphoma Society
- National Institutes of Health
- Albert Einstein College of Medicine, Yeshiva University
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Health Sciences › Medicine › Oncology › Cancer Treatment and Pharmacology
- Keywords
- kinesin-13, microtubule dynamics, microtubule depolymerization, dynamic instability, catastrophe, rescue, EB1, interphase, Drosophila melanogaster, S2 cells
- MeSH terms
- Animals, Cell Line, Drosophila, Drosophila melanogaster, Glutathione Transferase, Interphase, Microscopy, Fluorescence, Microtubules, Models, Biological, Polymers, RNA, Double-Stranded, Time Factors, Blotting, Western, Kinesins, Protein Structure, Tertiary, RNA Interference, Green Fluorescent Proteins
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