The Y-family DNA polymerase κ (pol κ) functions in mammalian nucleotide-excision repair
Abstract
DNA polymerase kappa (pol kappa) is a member of the Y-family of DNA polymerases that are thought to function in translesion synthesis (TLS) past different types of DNA damage. Here, we show that pol kappa-deficient mouse cells have substantially reduced (but not absent) levels of nucleotide excision repair (NER) of UV damage, as measured by several methods. Our results provide evidence for an unexpected role for pol kappa in mammalian NER.
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DNA polymerase κ directly participates in NER repair synthesis in human cells, and loss of TLS activity greatly potentiates the checkpoint response to UV in yeast G1 cells, suggesting closely opposing UV lesions may be at least partly responsible for checkpoint activation.
“Interestingly, it was recently shown that DNA polymerase κ directly participates to NER repair synthesis in human cells [70,71] and that loss of TLS activity greatly potentiates the checkpoint response to UV irradiation in yeast G1 cells [61], suggesting that closely opposing UV lesions may indeed be at least partly responsible for checkpoint activation.”
From Mind the gap: keeping UV lesions in check (2011) · cites this paper as [70]
In human cells, DNA polymerase κ is responsible for approximately 30% of unscheduled DNA synthesis detected in UV-irradiated cells.
“an unexpected role for TLS polymerases in NER was described in human cells, where DNA polymerase κ was found to be responsible for approximately 30% of the unscheduled DNA synthesis detected in UV-irradiated cells [70,71].”
From Mind the gap: keeping UV lesions in check (2011) · cites this paper as [70]
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- Authors
- Ogi, T., & Lehmann, A. R.
- Affiliations
- University of Sussex (GB)
- Corresponding author
- Lehmann, Alan R.
- Venue
- Nature Cell Biology
- Published
- 2006-06
- Volume
- 8
- Issue
- 6
- Pages
- 640-642
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- OpenAlex
- W1535018042
- Cited by
- 211
- References
- 15
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 10 · 2023: 4 · 2022: 4 · 2021: 10 · 2020: 5 · 2019: 4 · 2018: 9 · 2017: 18 · 2016: 9 · 2015: 10 · 2014: 9 · 2013: 11 · 2012: 12
- FWCI
- 7.29
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Uehara Memorial Foundation
- Medical Research Council
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
- Keywords
- DNA polymerase kappa, Y-family DNA polymerases, nucleotide excision repair, UV-induced DNA damage, translesion synthesis, DNA repair
- MeSH terms
- Y-Family DNA Polymerases, Animals, Cell Line, DNA-Directed DNA Polymerase, DNA Repair, Fibroblasts, Kinetics, Ultraviolet Rays, Mice
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