CoChat
Sign Up
Cited by 297

The DOI matches its Crossref record; 6 of the 6 references it cites were found in a registry or an index.

Clathrin and phosphatidylinositol-4,5-bisphosphate regulate autophagic lysosome reformation

Rong, Yueguang, Liu, Mei, Ma, Liang, Du, Wanqing, Zhang, Hanshuo, Tian, Yuan, Cao, Zhen, Li, Ying, Ren, He, Zhang, Chuanmao, Li, Lin, Chen, She, Xi, Jianzhong, Yu, Li·2012·Nature Cell Biology
DOI 10.1038/ncb2557
Open this paper in CoChat — free account

Abstract

Autophagy is a lysosome-based degradation pathway. During autophagy, lysosomes fuse with autophagosomes to form autolysosomes. Following starvation-induced autophagy, nascent lysosomes are formed from autolysosomal membranes through an evolutionarily conserved cellular process, autophagic lysosome reformation (ALR), which is critical for maintaining lysosome homeostasis. Here we report that clathrin and phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P(2)) regulate ALR. Combining a screen of candidates identified through proteomic analysis of purified ALR tubules, and large-scale RNAi knockdown, we unveiled a tightly regulated molecular pathway that controls lysosome homeostasis, in which clathrin and PtdIns(4,5)P(2) are the central components. Our functional study demonstrates the central role of clathrin and its associated proteins in cargo sorting, phospholipid conversion, initiation of autolysosome tubulation, and proto-lysosome budding during ALR. Our data not only uncover a molecular pathway by which lysosome homeostasis is maintained through the ALR process, but also reveal unexpected functions of clathrin and PtdIns(4,5)P(2) in lysosome homeostasis.

Overview

Integrity signals

Registry

Crossref · 10.1038/ncb2557

Cites

6 of 6 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Citations

Cites

Flagged first, then by citations

Research works printed but not linked: 23

  1. Klionsky, D. J. Autophagy: from phenomenology to molecular understanding in less than a decade. Nat. Rev. Mol. Cell Biol. 8, 931–937 (2007). · DOI
  2. Mizushima, N. Autophagy: process and function. Genes Dev. 21, 2861–2873 (2007). · DOI
  3. Kraft, C. & Martens, S. Mechanisms and regulation of autophagosome formation. Curr. Opin. Cell Biol. http://dx.doi.org/10.1016/j.ceb.2012.05.001 (2012). · DOI
  4. Klionsky, D. J. & Emr, S. D. Autophagy as a regulated pathway of cellular degradation. Science 290, 1717–1721 (2000). · DOI
  5. Zhang, H. et al. Genome-wide functional screening of miR-23b asa pleiotropic modulator suppressing cancer metastasis. Nat. Commun. 2, 554 (2011). · DOI
  6. Pawlowski, N. Dynamin self-assembly and the vesicle scission mechanism: how dynamin oligomers cleave the membrane neck of clathrin-coated pits during endocytosis. Bioessays 32, 1033–1039 (2010). · DOI
  7. Raiborg, C. et al. Hrs sorts ubiquitinated proteins into clathrin-coated microdomains of early endosomes. Nat. Cell Biol. 4, 394–398 (2002). · DOI · Paper page
  8. Popoff, V. et al. The retromer complex and clathrin define an early endosomal retrograde exit site. J. Cell Sci. 120, 2022–2031 (2007). · DOI
  9. Shi, A. et al. Regulation of endosomal clathrin and retromer-mediated endosome to Golgi retrograde transport by the J-domain protein RME-8. EMBO J. 28, 3290–3302 (2009). · DOI
  10. Di Paolo, G. & De Camilli, P. Phosphoinositides in cell regulation and membrane dynamics. Nature 443, 651–657 (2006). · DOI · Paper page
  11. Divecha, N. & Irvine, R. F. Phospholipid signaling. Cell 80, 269–278 (1995). · DOI
  12. Funakoshi, Y., Hasegawa, H. & Kanaho, Y. Activation mechanisms of PIP5K isozymes by the small GTPase ARF6. Adv. Enzyme Regul. 50, 72–80 (2010). · DOI
  13. van den Bout, I. & Divecha, N. PIP5K-driven PtdIns(4,5)P2 synthesis: regulation and cellular functions. J. Cell Sci. 122, 3837–3850 (2009). · DOI
  14. Boronenkov, I. V. & Anderson, R. A. The sequence of phosphatidylinositol-4-phosphate 5-kinase defines a novel family of lipid kinases. J. Biol. Chem. 270, 2881–2884 (1995). · DOI
  15. Rohde, G., Wenzel, D. & Haucke, V. A phosphatidylinositol (4,5)-bisphosphate binding site within mu2-adaptin regulates clathrin-mediated endocytosis. J. Cell Biol. 158, 209–214 (2002). · DOI
  16. Levine, T. P. & Munro, S. Targeting of Golgi-specific pleckstrin homology domains involves both PtdIns 4-kinase-dependent and -independent components. Curr. Biol. 12, 695–704 (2002). · DOI
  17. Lehto, M. & Olkkonen, V. M. The OSBP-related proteins: a novel protein family involved in vesicle transport, cellular lipid metabolism, and cell signalling. Biochim. Biophys. Acta 1631, 1–11 (2003). · DOI
  18. Ford, M. G. J. et al. Simultaneous binding of PtdIns(4,5)P2 and clathrin by AP180 in the nucleation of clathrin lattices on membranes. Science 291, 1051–1055 (2001). · DOI · Paper page
  19. Smythe, E., Carter, L. L. & Schmid, S. L. Cytosol- and clathrin-dependent stimulation of endocytosis in vitro by purified adaptors. J. Cell Biol. 119, 1163–1171 (1992). · DOI
  20. McNiven, M. A. & Thompson, H. M. Vesicle formation at the plasma membrane and trans-golgi network: the same but different. Science 313, 1591–1594 (2006). · DOI
  21. Schmid, S. L. Clathrin-coated vesicle formation and protein sorting: an integrated process. Annu. Rev. Biochem. 66, 511–548 (1997). · DOI
  22. Traub, L. M. et al. AP-2-containing clathrin coats assemble on mature lysosomes. J. Cell Biol. 135, 1801–1814 (1996). · DOI
  23. Popoff, V. et al. Analysis of articulation between clathrin and retromer in retrograde sorting on early endosomes. Traffic 10, 1868–1880 (2009). · DOI

Cited by

Sorted by citations

Metadata

Authors
Rong, Y., Liu, M., Ma, L., Du, W., Zhang, H., Tian, Y., Cao, Z., Li, Y., Ren, H., Zhang, C., Li, L., Chen, S., Xi, J., & Yu, L.
Venue
Nature Cell Biology
Publisher
Springer Science and Business Media LLC
Published
2012-08-12
Volume
14
Issue
9
Pages
924-934
ISSN
  • 1465-7392
  • 1476-4679
DOI
10.1038/ncb2557
Cited by
297
References
6
Language
en
Licence
www.springer.com/tdm (tdm)

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review