Cited by 387Calcium signaling and nucleotide metabolismCellular transport and secretionAutophagy in Disease and Therapy

A molecular mechanism to regulate lysosome motility for lysosome positioning and tubulation

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Li, Xinran, Rydzewski, Nicholas, Hider, Ahmad, Zhang, Xiaoli, Yang, Junsheng, Wang, Wuyang, Gao, Qiong, Cheng, Xiping +1 more·2016·Nature Cell Biology

Abstract

To mediate the degradation of biomacromolecules, lysosomes must traffic towards cargo-carrying vesicles for subsequent membrane fusion or fission. Mutations of the lysosomal Ca(2+) channel TRPML1 cause lysosomal storage disease (LSD) characterized by disordered lysosomal membrane trafficking in cells. Here we show that TRPML1 activity is required to promote Ca(2+)-dependent centripetal movement of lysosomes towards the perinuclear region (where autophagosomes accumulate) following autophagy induction. ALG-2, an EF-hand-containing protein, serves as a lysosomal Ca(2+) sensor that associates physically with the minus-end-directed dynactin-dynein motor, while PtdIns(3,5)P(2), a lysosome-localized phosphoinositide, acts upstream of TRPML1. Furthermore, the PtdIns(3,5)P(2)-TRPML1-ALG-2-dynein signalling is necessary for lysosome tubulation and reformation. In contrast, the TRPML1 pathway is not required for the perinuclear accumulation of lysosomes observed in many LSDs, which is instead likely to be caused by secondary cholesterol accumulation that constitutively activates Rab7-RILP-dependent retrograde transport. Ca(2+) release from lysosomes thus provides an on-demand mechanism regulating lysosome motility, positioning and tubulation.

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  1. [29]Schroer TA Dynactin. Annual review of cell and developmental biology 2004 20 759 779 15473859

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Authors
Li, X., Rydzewski, N., Hider, A., Zhang, X., Yang, J., Wang, W., Gao, Q., Cheng, X., & Xu, H.
Affiliations
  • University of Michigan (US)
  • Zhejiang University of Technology (CN)
Corresponding author
Xu, Haoxing
Venue
Nature Cell Biology
Published
2016-03-07
Volume
18
Issue
4
Pages
404-417
ISSN
  • 1465-7392 (ISSN-L)
  • 1476-4679
OpenAlex
W2299614729
Cited by
387
References
146
Citations by year
2026: 26 · 2025: 25 · 2024: 38 · 2023: 36 · 2022: 46 · 2021: 44 · 2020: 42 · 2019: 30 · 2018: 44 · 2017: 39 · 2016: 17
FWCI
39.52
Type
journal article
Language
en
Open access
Locations
  • Nature Cell Biology (publishedVersion)
  • PubMed (publishedVersion)
  • PubMed Central (acceptedVersion)
  • Deep Blue (University of Michigan) (submittedVersion)
Funders
  • National Institute of Mental Health (MH096595, R03MH096595)
  • National Institute of Neurological Disorders and Stroke (R21NS091928, NS062792, R01NS062792)
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (AR060837, R01AR060837)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
Keywords
lysosome motility, lysosome positioning, TRPML1, lysosomal storage disease, ALG-2, PtdIns(3,5)P2, autophagy, lysosomal calcium release, retrograde transport, cholesterol accumulation, perinuclear accumulation
MeSH terms
rab7 GTP-Binding Proteins, Animals, Autophagy, Base Sequence, Calcium, Calcium-Binding Proteins, Cell Line, Chlorocebus aethiops, Dyneins, HeLa Cells, Humans, Luminescent Proteins, Lysosomes, Microscopy, Fluorescence, Models, Biological, Molecular Sequence Data, Mutation, Signal Transduction, Phosphatidylinositol Phosphates, Mice, Knockout, COS Cells, rab GTP-Binding Proteins, Fluorescence Recovery After Photobleaching, Adaptor Proteins, Signal Transducing, Transient Receptor Potential Channels, Apoptosis Regulatory Proteins, HEK293 Cells

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