SOX17 links gut endoderm morphogenesis and germ layer segregation
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Gastrulation leads to three germ layers--ectoderm, mesoderm and endoderm--that are separated by two basement membranes. In the mouse embryo, the emergent gut endoderm results from the widespread intercalation of cells of two distinct origins: pluripotent epiblast-derived definitive endoderm (DE) and extra-embryonic visceral endoderm (VE). Here we image the trajectory of prospective DE cells before intercalating into the VE epithelium. We show that the transcription factor SOX17, which is activated in prospective DE cells before intercalation, is necessary for gut endoderm morphogenesis and the assembly of the basement membrane that separates gut endoderm from mesoderm. Our results mechanistically link gut endoderm morphogenesis and germ layer segregation, two central and conserved features of gastrulation.
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The gut endoderm is comprised of cells of both PrE and DE.
“Our previous studies revealed the gut endoderm to be comprised of cells of both PrE and DE (Extended Data Fig. 1a)5–7.”
From The emergent landscape of the mouse gut endoderm at single-cell resolution (2019) · cites this paper as [5]
At gastrulation (E7.0-E7.5), EPI-derived DE cells intercalate into the emVE epithelium to form the gut endoderm.
“At gastrulation (E7.0-E7.5), EPI-derived (embryonic) DE cells intercalate into the emVE epithelium to form the gut endoderm5,7.”
From The emergent landscape of the mouse gut endoderm at single-cell resolution (2019) · cites this paper as [5]
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Self-citation
- Authors
- Viotti, M., Nowotschin, S., & Hadjantonakis, A.-K.
- Affiliations
- Cornell University (US)
- Corresponding author
- Hadjantonakis, Anna-Katerina
- Venue
- Nature Cell Biology
- Published
- 2014-11-24
- Volume
- 16
- Issue
- 12
- Pages
- 1146-1156
- ISSN
- 1465-7392 (ISSN-L)
- 1476-4679
- PMC
- PMC4250291
- OpenAlex
- W2021593900
- Semantic Scholar
- 208e07481420494ae5e3cae7671f4899d6e0f0de
- Cited by
- 150
- References
- 39
- Citations by year
- 2026: 11 · 2025: 13 · 2024: 9 · 2023: 6 · 2022: 16 · 2021: 12 · 2020: 15 · 2019: 13 · 2018: 14 · 2017: 15 · 2016: 12 · 2015: 13 · 2014: 1
- FWCI
- 5.57
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Cell Biology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Cancer Institute (P30CA008748)
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK084391)
- Eunice Kennedy Shriver National Institute of Child Health and Human Development (R01HD052115)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Renal and related cancers
- Keywords
- SOX17, gastrulation, definitive endoderm, visceral endoderm, basement membrane assembly, cell intercalation, epiblast, transcription factors, mouse embryo
- MeSH terms
- Animals, Basement Membrane, Cell Differentiation, Embryo, Mammalian, Endoderm, Epithelium, Extracellular Matrix, Fibronectins, Germ Layers, Mesoderm, Mice, Transgenic, Morphogenesis, Cadherins, Extracellular Matrix Proteins, HMGB Proteins, Green Fluorescent Proteins, Mice, Hepatocyte Nuclear Factor 3-beta, Gastrulation, SOXF Transcription Factors, Optical Imaging
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