Mussel protein adhesion depends on interprotein thiol-mediated redox modulation
Open accessAbstract
Mussel adhesion is mediated by foot proteins (mfps) rich in a catecholic amino acid, 3,4-dihydroxyphenylalanine (dopa), capable of forming strong bidentate interactions with a variety of surfaces. A tendency toward facile auto-oxidation, however, often renders dopa unreliable for adhesion. We demonstrate that mussels limit dopa oxidation during adhesive plaque formation by imposing an acidic, reducing regime based on the thiol-rich mfp-6, which restores dopa by coupling the oxidation of thiols to dopaquinone reduction.
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The catechol group is prone to oxidation, which may reduce adhesive performance and induce accidental crosslinking.
“First, the catechol group is prone to oxidation. The oxidation of catechol may reduce the adhesive performance of mussel-inspired hydrogels, especially for some mineral or metal substrates, and is prone to inducing accidental crosslinking [155].”
From Mussel-Inspired Injectable Adhesive Hydrogels for Biomedical Applications (2024) · cites this paper as [155]
The opening passage following the Abstract states that dopa readily oxidizes to dopaquinone, diminishing adhesion to titania and mica by at least 80% and making further reactions, including cross-linking, likely. The paper supports the proposed mechanism, but the cited passage does not establish reduced adhesive performance specifically in mussel-inspired hydrogels. (LLM, conf=0.84)
Experimental — accuracy not yet measuredLong-term storage of precursor solutions of mussel-inspired hydrogels is usually difficult.
“The long-term storage of precursor solutions of mussel-inspired hydrogels is usually difficult.”
From Mussel-Inspired Injectable Adhesive Hydrogels for Biomedical Applications (2024) · cites this paper as [155]
The Abstract says dopa readily oxidizes and that this presents challenges for its use in synthetic polymers with dopa-like side chains. It does not address mussel-inspired hydrogel precursor solutions or say that their long-term storage is usually difficult; searches for storage and hydrogel precursors found no such passage. (LLM, conf=0.76)
Experimental — accuracy not yet measuredThe thiol group can restore dopa from oxidized dopaquinone, benefiting preservation of catechol groups.
“The thiol group has been reported to be able to restore dopa from oxidized dopaquinone [155].”
From Mussel-Inspired Injectable Adhesive Hydrogels for Biomedical Applications (2024) · cites this paper as [155]
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Cites
Thiol-based redox switches in eukaryotic proteins
Brandes, N., Schmitt, S., & Jakob, U. (2009) · Antioxidants & Redox Signaling · doi:10.1089/ars.2008.2285 · Cited by 373 · see citing works
Flagged first, then by citations
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Cited by
Sorted by citations
Mussel-Inspired Adhesives and Coatings
Lee, B. P., Messersmith, P. B., Israelachvili, J. N., & Waite, J. H. (2011) · Annual Review of Materials Research · doi:10.1146/annurev-matsci-062910-100429 · Cited by 1,668 · see citing works
Self-citationBiological materials: Functional adaptations and bioinspired designs
Chen, P.-Y., McKittrick, J., & Meyers, M. A. (2012) · Progress in Materials Science · doi:10.1016/j.pmatsci.2012.03.001 · Cited by 766 · see citing works
Adaptive synergy between catechol and lysine promotes wet adhesion by surface salt displacement
Maier, G. P., Rapp, M. V., Waite, J. H., Israelachvili, J. N., & Butler, A. (2015) · Science · doi:10.1126/science.aab0556 · Cited by 722 · see citing works
Self-citationRecent progress in synthesis and application of mussel-inspired adhesives
Guo, Q., Chen, J., Wang, J., Zeng, H., & Yu, J. (2020) · Nanoscale · doi:10.1039/c9nr09780e · Cited by 370 · see citing works
Self-citationLiu, Y., Zhu, Z., Pei, X., Zhang, X., Cheng, X., Hu, S., Gao, X., Wang, J., Chen, J., & Wan, Q. (2020) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.0c12090 · Cited by 259 · see citing works
Design principles for creating synthetic underwater adhesives
Narayanan, A., Dhinojwala, A., & Joy, A. (2021) · Chemical Society Reviews · doi:10.1039/d1cs00316j · Cited by 193 · see citing works
Wang, B., Liu, J., Niu, D., Wu, N., Yun, W., Wang, W., Zhang, K., Li, G., Yan, S., Xu, G., et al. (2021) · ACS Applied Materials & Interfaces · doi:10.1021/acsami.1c06058 · Cited by 109 · see citing works
Mussel-Inspired Injectable Adhesive Hydrogels for Biomedical Applications
Dou, W., Zeng, X., Zhu, S., Zhu, Y., Liu, H., & Li, S. (2024) · International Journal of Molecular Sciences · doi:10.3390/ijms25169100 · Cited by 52 · see citing works
“The thiol group has been reported to be able to restore dopa from oxidized dopaquinone [155].”
Background
- Authors
- Yu, J., Wei, W., Danner, E., Ashley, R. K., Israelachvili, J. N., & Waite, J. H.
- Affiliations
- University of California, Santa Barbara (US)
- Venue
- Nature Chemical Biology
- Published
- 2011-07-31
- Volume
- 7
- Issue
- 9
- Pages
- 588-590
- ISSN
- 1552-4450 (ISSN-L)
- 1552-4469
- PMC
- PMC3158268
- OpenAlex
- W1992011929
- Cited by
- 463
- References
- 1
- Citations by year
- 2026: 24 · 2025: 35 · 2024: 31 · 2023: 28 · 2022: 41 · 2021: 33 · 2020: 41 · 2019: 33 · 2018: 30 · 2017: 37 · 2016: 30 · 2015: 22 · 2014: 37 · 2013: 18 · 2012: 20
- FWCI
- 11.53
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Chemical Biology (publishedVersion)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- eScholarship (California Digital Library) (submittedVersion)
- Funders
- National Science Foundation (R01 DE018468)
- National Institutes of Health
- Materials Research Science and Engineering Center, Harvard University
- National Institute for Materials Science
- National Institute of Dental and Craniofacial Research (R01DE018468)
- Topics
- Physical Sciences › Materials Science › Surfaces, Coatings and Films › Polymer Surface Interaction Studies
- Physical Sciences › Engineering › Ocean Engineering › Marine Biology and Environmental Chemistry
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biochemical and Structural Characterization
- Keywords
- mussel foot proteins, mussel adhesion, DOPA, 3,4-dihydroxyphenylalanine, autoxidation
- MeSH terms
- Adhesiveness, Amino Acid Sequence, Animals, Dihydroxyphenylalanine, Molecular Sequence Data, Oxidation-Reduction, Proteins, Sulfhydryl Compounds, Benzoquinones, Bivalvia
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