Optogenetic activation of Plexin-B1 reveals contact repulsion between osteoclasts and osteoblasts
Open accessAbstract
During bone remodelling, osteoclasts induce chemotaxis of osteoblasts and yet maintain spatial segregation. We show that osteoclasts express the repulsive guidance factor Semaphorin 4D and induce contact inhibition of locomotion (CIL) in osteoblasts through its receptor Plexin-B1. To examine causality and elucidate how localized Plexin-B1 stimulation may spatiotemporally coordinate its downstream targets in guiding cell migration, we develop an optogenetic tool for Plexin-B1 designated optoPlexin. Precise optoPlexin activation at the leading edge of migrating osteoblasts readily induces local retraction and, unexpectedly, distal protrusions to steer cells away. These morphological changes are accompanied by reorganization of Myosin II, PIP 3, adhesion and active Cdc42. We attribute the resultant repolarization to RhoA/ROCK-mediated redistribution of β-Pix, which activates Cdc42 and promotes protrusion. Thus, our data demonstrate a causal role of Plexin-B1 for CIL in osteoblasts and reveals a previously unknown effect of Semaphorin signalling on spatial distribution of an activator of cell migration.
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Recent studies have unveiled a role of Plexins in cell collision guidance.
“Plexin signaling is best known for growth cone collapse during neurodevelopment; recent studies from our laboratory and others have further unveiled a role of Plexins in cell collision guidance58, 59.”
From Regulation of cell distancing in peri-plaque glial nets by Plexin-B1 affects glial activation and amyloid compaction in Alzheimer’s disease (2024) · cites this paper as [58]
Contact inhibition of locomotion has been reported for Plexin-B1 mediated physical contact between osteoclasts and osteoblasts.
“This process, termed contact inhibition of locomotion (CIL), has also been reported for Plexin-B1 mediated physical contact between osteoclasts and osteoblasts58.”
From Regulation of cell distancing in peri-plaque glial nets by Plexin-B1 affects glial activation and amyloid compaction in Alzheimer’s disease (2024) · cites this paper as [58]
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Cites
Improved vectors and genome-wide libraries for CRISPR screening
Sanjana, N. E., Shalem, O., & Zhang, F. (2014) · Nature Methods · doi:10.1038/nmeth.3047 · Cited by 5,239 · see citing works
Phosphoinositides in cell regulation and membrane dynamics
Di Paolo, G., & De Camilli, P. (2006) · Nature · doi:10.1038/nature05185 · Cited by 2,790 · see citing works
Non-muscle myosin II takes centre stage in cell adhesion and migration
Vicente‐Manzanares, M., Ma, X., Adelstein, R. S., & Horwitz, A. R. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2786 · Cited by 2,041 · see citing works
TGF-β1–induced migration of bone mesenchymal stem cells couples bone resorption with formation
Tang, Y., Wu, X., Lei, W., Pang, L., Wan, C., Shi, Z., Zhao, L., Nagy, T. R., Peng, X., Hu, J., et al. (2009) · Nature Medicine · doi:10.1038/nm.1979 · Cited by 1,165 · see citing works
Rapid blue-light–mediated induction of protein interactions in living cells
Kennedy, M. J., Hughes, R. M., Peteya, L. A., Schwartz, J. W., Ehlers, M., & Tucker, C. L. (2010) · Nature Methods · doi:10.1038/nmeth.1524 · Cited by 1,126 · see citing works
Photoexcited CRY2 Interacts with CIB1 to Regulate Transcription and Floral Initiation in Arabidopsis
Liu, H., Yu, X., Li, K., John Klejnot, B., Yang, H., Lisiero, D. N., & Lin, C. (2008) · Science · doi:10.1126/science.1163927 · Cited by 720 · see citing works
Temporally precise in vivo control of intracellular signalling
Airan, R. D., Thompson, K. R., Fenno, L. E., Bernstein, H. L., & Deisseroth, K. A. (2009) · Nature · doi:10.1038/nature07926 · Cited by 715 · see citing works
Observations on the social behaviour of cells in tissue culture
Abercrombie, M., & Heaysman, J. E. M. (1954) · Experimental Cell Research · doi:10.1016/0014-4827(54)90176-7 · Cited by 621 · see citing works
Kuo, J., Han, X., Hsiao, C.-T., Yates, J. R., & Waterman, C. M. (2011) · Nature Cell Biology · doi:10.1038/ncb2216 · Cited by 591 · see citing works
Using Optogenetics to Interrogate the Dynamic Control of Signal Transmission by the Ras/Erk Module
Toettcher, J. E., Weiner, O. D., & Lim, W. A. (2013) · Cell · doi:10.1016/j.cell.2013.11.004 · Cited by 539 · see citing works
Suppression of bone formation by osteoclastic expression of semaphorin 4D
Negishi-Koga, T., Shinohara, M., Komatsu, N., Bito, H., Kodama, T., Friedel, R. H., & Takayanagi, H. (2011) · Nature Medicine · doi:10.1038/nm.2489 · Cited by 498 · see citing works
Observations on the social behaviour of cells in tissue culture
Abercrombie, M., & Heaysman, J. E. M. (1953) · Experimental Cell Research · doi:10.1016/0014-4827(53)90098-6 · Cited by 497 · see citing works
Semaphorins command cells to move
Kruger, R. P., Aurandt, J., & Guan, K. (2005) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1740 · Cited by 496 · see citing works
Regulation of PTEN by Rho small GTPases
Zhong, L., Dong, X., Wang, Z., Liu, W., Deng, N., Ding, Y., Tang, L.-Y., Hla, T., Zeng, R., Li, L., et al. (2005) · Nature Cell Biology · doi:10.1038/ncb1236 · Cited by 488 · see citing works
Optogenetic protein clustering and signaling activation in mammalian cells
Bugaj, L. J., Choksi, A. T., Mesuda, C. K., Kane, R. S., & Schaffer, D. V. (2013) · Nature Methods · doi:10.1038/nmeth.2360 · Cited by 456 · see citing works
The Semaphorin 4D Receptor Plexin-B1 Is a GTPase Activating Protein for R-Ras
Oinuma, I., Ishikawa, Y., Katoh, H., & Negishi, M. (2004) · Science · doi:10.1126/science.1097545 · Cited by 383 · see citing works
Plexin-B1 Directly Interacts with PDZ-RhoGEF/LARG to Regulate RhoA and Growth Cone Morphology
Swiercz, J. M., Kuner, R., Behrens, J., & Offermanns, S. (2002) · Neuron · doi:10.1016/s0896-6273(02)00750-x · Cited by 360 · see citing works
Chase-and-run between adjacent cell populations promotes directional collective migration
Théveneau, E., Steventon, B., Scarpa, E., García, S., Trepat, X., Streit, A., & Mayor, R. (2013) · Nature Cell Biology · doi:10.1038/ncb2772 · Cited by 321 · see citing works
Rho protein crosstalk: another social network?
Guilluy, C., García‐Mata, R., & Burridge, K. (2011) · Trends in Cell Biology · doi:10.1016/j.tcb.2011.08.002 · Cited by 290 · see citing works
Scarpa, E., Szabó, A., Bibonne, A., Théveneau, E., Parsons, M., & Mayor, R. (2015) · Developmental Cell · doi:10.1016/j.devcel.2015.06.012 · Cited by 279 · see citing works
Targeting and activation of Rac1 are mediated by the exchange factor β-Pix
ten Klooster, J. P., Jaffer, Z. M., Chernoff, J., & Hordijk, P. L. (2006) · The Journal of Cell Biology · doi:10.1083/jcb.200509096 · Cited by 250 · see citing works
Astin, J. W., Batson, J., Kadir, S. R., Charlet, J., Persad, R. A., Gillatt, D. A., Oxley, J., & Nobes, C. D. (2010) · Nature Cell Biology · doi:10.1038/ncb2122 · Cited by 245 · see citing works
Structural basis of semaphorin–plexin signalling
Janssen, B. J., Robinson, R., Pérez-Brangulí, F., Bell, C. H., Mitchell, K., Siebold, C., & Jones, E. Y. (2010) · Nature · doi:10.1038/nature09468 · Cited by 236 · see citing works
MicroRNA-27a/b controls endothelial cell repulsion and angiogenesis by targeting semaphorin 6A
Urbich, C., Kaluza, D., Frömel, T., Knau, A., Bennewitz, K., Boon, R. A., Bonauer, A., Doebele, C., Boeckel, J., Hergenreider, E., et al. (2011) · Blood · doi:10.1182/blood-2011-08-373886 · Cited by 231 · see citing works
Structural basis for semaphorin signalling through the plexin receptor
Nogi, T., Yasui, N., Mihara, E., Matsunaga, Y., Noda, M., Yamashita, N., Toyofuku, T., Uchiyama, S., Goshima, Y., Kumanogoh, A., et al. (2010) · Nature · doi:10.1038/nature09473 · Cited by 225 · see citing works
Mechanisms and in vivo functions of contact inhibition of locomotion
Stramer, B. M., & Mayor, R. (2016) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm.2016.118 · Cited by 211 · see citing works
Early contacts between fibroblasts
Heaysman, J. E. M., & Pegrum, S. M. (1973) · Experimental Cell Research · doi:10.1016/0014-4827(73)90039-6 · Cited by 197 · see citing works
An Eph receptor regulates integrin activity through R-Ras
Zou, J. X., Wang, B., Kalo, M. S., Zisch, A. H., Pasquale, E. B., & Ruoslahti, E. I. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.24.13813 · Cited by 192 · see citing works
Driessens, M. H. E., Hu, H., Nobes, C. D., Self, A. J., Jordens, I., Goodman, C. S., & Hall, A. (2001) · Current Biology · doi:10.1016/s0960-9822(01)00092-6 · Cited by 192 · see citing works
Füller, T., Korff, T., Kilian, A., Dandekar, G., & Augustin, H. G. (2003) · Journal of Cell Science · doi:10.1242/jcs.00426 · Cited by 172 · see citing works
Hota, P. K., & Buck, M. (2012) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-012-1019-0 · Cited by 170 · see citing works
Aurandt, J., Vikis, H. G., Gutkind, J. S., Ahn, N. G., & Guan, K. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.142433199 · Cited by 166 · see citing works
Plexin-B1/RhoGEF–mediated RhoA activation involves the receptor tyrosine kinase ErbB-2
Swiercz, J. M., Kuner, R., & Offermanns, S. (2004) · The Journal of Cell Biology · doi:10.1083/jcb.200312094 · Cited by 165 · see citing works
Light-inducible receptor tyrosine kinases that regulate neurotrophin signalling
Chang, K.-Y., Woo, D., Jung, H., Lee, S., Kim, S., Won, J., Kyung, T., Park, H., Kim, N., Yang, H. W., et al. (2014) · Nature Communications · doi:10.1038/ncomms5057 · Cited by 148 · see citing works
Basile, J. R., Afkhami, T., & Gutkind, J. S. (2005) · Molecular and Cellular Biology · doi:10.1128/mcb.25.16.6889-6898.2005 · Cited by 145 · see citing works
Tong, Y., Chugha, P., Hota, P. K., Alviani, R. S., Li, M., Tempel, W., Shen, L., Park, H.-W., & Buck, M. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m703800200 · Cited by 137 · see citing works
Oinuma, I., Katoh, H., & Negishi, M. (2004) · Journal of Neuroscience · doi:10.1523/jneurosci.3257-04.2004 · Cited by 129 · see citing works
Osteoclasts Control Osteoblast Chemotaxis via PDGF-BB/PDGF Receptor Beta Signaling
Sanchez-Fernandez, M. A., Gallois, A., Riedl, T., Jurdic, P., & Hoflack, B. (2008) · PLoS ONE · doi:10.1371/journal.pone.0003537 · Cited by 128 · see citing works
Shin, E., Shin, K., Lee, C., Woo, K.-N., Quan, S., Soung, N., Kim, Y. G., Ik, C., Kim, S., Park, D., et al. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m203754200 · Cited by 127 · see citing works
Basile, J. R., Gavard, J., & Gutkind, J. S. (2007) · Journal of Biological Chemistry · doi:10.1074/jbc.m705467200 · Cited by 126 · see citing works
Oinuma, I., Katoh, H., Harada, A., & Negishi, M. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m303047200 · Cited by 124 · see citing works
Blue-Light-Induced Changes in Arabidopsis Cryptochrome 1 Probed by FTIR Difference Spectroscopy
Kottke, T., Batschauer, A., Ahmad, M., & Heberle, J. (2006) · Biochemistry · doi:10.1021/bi051964b · Cited by 118 · see citing works
Stramer, B. M., Moreira, S., Millard, T. H., Evans, I. R., Huang, C.-Y., Sabet, O., Milner, M., Dunn, G., Martin, P. B., & Wood, W. (2010) · The Journal of Cell Biology · doi:10.1083/jcb.200912134 · Cited by 115 · see citing works
Inter-Cellular Forces Orchestrate Contact Inhibition of Locomotion
Davis, J. R., Luchici, A., Mosis, F., Thackery, J., Salazar, J. A., Mao, Y., Dunn, G., Betz, T., Miodownik, M. A., & Stramer, B. M. (2015) · Cell · doi:10.1016/j.cell.2015.02.015 · Cited by 115 · see citing works
Optogenetic control of chemokine receptor signal and T-cell migration
Xu, Y., Hyun, Y., Lim, K., Lee, H., Cummings, R. J., Gerber, S. A., Bae, S., Cho, T. Y., Lord, E. M., & Kim, M. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1319296111 · Cited by 111 · see citing works
Chemotaxis of human osteoblasts
Lind, M., Deleuran, B. W., Thestrup‐Pedersen, K., Søballé, K., Eriksen, E. F., & Bünger, C. E. (1995) · Apmis · doi:10.1111/j.1699-0463.1995.tb01089.x · Cited by 110 · see citing works
Contact Repulsion Controls the Dispersion and Final Distribution of Cajal-Retzius Cells
Villar‐Cerviño, V., Molano‐Mazón, M., Catchpole, T., Valdeolmillos, M., Henkemeyer, M. J., Martı́nez, L. M., Borrell, V., & Marín, Ó. (2013) · Neuron · doi:10.1016/j.neuron.2012.11.023 · Cited by 108 · see citing works
Migrating fibroblasts reorient directionality by a metastable, PI3K-dependent mechanism
Welf, E. S., Ahmed, S., Johnson, H. E., Melvin, A. T., & Haugh, J. M. (2012) · The Journal of Cell Biology · doi:10.1083/jcb.201108152 · Cited by 100 · see citing works
Chemotactic response of osteoblast-like cells to transforming growth factor beta
Lucas, P. A. (1989) · Bone · doi:10.1016/8756-3282(89)90079-3 · Cited by 96 · see citing works
Kutys, M. L., & Yamada, K. M. (2014) · Nature Cell Biology · doi:10.1038/ncb3026 · Cited by 91 · see citing works
Schaefer, G., Shao, L., Tótpál, K., & Akita, R. W. (2007) · Cancer Research · doi:10.1158/0008-5472.can-06-3493 · Cited by 87 · see citing works
Interaction of plexin-B1 with PDZ domain-containing Rho guanine nucleotide exchange factors
Hirotani, M., Ohoka, Y., Yamamoto, T., Nirasawa, H., Furuyama, T., Kogo, M., Matsuya, T., & Inagaki, S. (2002) · Biochemical and Biophysical Research Communications · doi:10.1016/s0006-291x(02)02122-8 · Cited by 87 · see citing works
Directional Collective Cell Migration Emerges as a Property of Cell Interactions
Woods, M. L., Carmona‐Fontaine, C., Barnes, C. P., Couzin, I. D., Mayor, R., & Page, K. M. (2014) · PLoS ONE · doi:10.1371/journal.pone.0104969 · Cited by 81 · see citing works
B plexins activate Rho through PDZ‐RhoGEF
Driessens, M. H. E., Olivo, C., Nagata, K., Inagaki, M., & Collard, J. G. (2002) · FEBS Letters · doi:10.1016/s0014-5793(02)03323-9 · Cited by 81 · see citing works
Dynamics of contacts between lamellae of fibroblasts: Essential role of the actin cytoskeleton
Gloushankova, N. A., Krendel, M., Alieva, N. O., Bonder, E. M., Feder, H. H., Vasiliev, J. M., & Gelfand, I. M. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.8.4362 · Cited by 74 · see citing works
Control of Bone Resorption by Semaphorin 4D Is Dependent on Ovarian Function
Dacquin, R., Domenget, C., Kumanogoh, A., Kikutani, H., Jurdic, P., & Machuca‐Gayet, I. (2011) · PLoS ONE · doi:10.1371/journal.pone.0026627 · Cited by 73 · see citing works
Che, D. L., Duan, L., Zhang, K., & Cui, B. (2015) · ACS Synthetic Biology · doi:10.1021/acssynbio.5b00048 · Cited by 73 · see citing works
Eph receptors inactivate R-Ras through different mechanisms to achieve cell repulsion
Dail, M., Richter, M., Godement, P., & Pasquale, E. B. (2006) · Journal of Cell Science · doi:10.1242/jcs.02842 · Cited by 72 · see citing works
(Pre-)Osteoclasts Induce Retraction of Osteoblasts Before Their Fusion to Osteoclasts
Perez-Amodio, S., Beertsen, W., & Everts, V (2004) · Journal of Bone and Mineral Research · doi:10.1359/jbmr.040509 · Cited by 70 · see citing works
Design and Optimization of Genetically Encoded Fluorescent Biosensors: GTPase Biosensors
Hodgson, L., Pertz, O. C., & Hahn, K. M. (2008) · Methods in cell biology · doi:10.1016/s0091-679x(08)85004-2 · Cited by 66 · see citing works
Hense, A., Herman, E., Oldemeyer, S., & Kottke, T. (2014) · Journal of Biological Chemistry · doi:10.1074/jbc.m114.606327 · Cited by 64 · see citing works
Oinuma, I., Ito, Y., Katoh, H., & Negishi, M. (2010) · Journal of Biological Chemistry · doi:10.1074/jbc.m110.147546 · Cited by 60 · see citing works
A novel method to study contact inhibition of locomotion using micropatterned substrates
Scarpa, E., Roycroft, A., Théveneau, E., Terriac, E., Piel, M., & Mayor, R. (2013) · Biology Open · doi:10.1242/bio.20135504 · Cited by 58 · see citing works
A Dual Binding Mode for RhoGTPases in Plexin Signalling
Bell, C., Aricescu, A. R., Jones, E. Y., & Siebold, C. (2011) · PLoS Biology · doi:10.1371/journal.pbio.1001134 · Cited by 58 · see citing works
Kato, K., Yazawa, T., Taki, K., Mori, K., Wang, S., Nishioka, T., Hamaguchi, T., Itoh, T., Takenawa, T., Kataoka, C., et al. (2012) · Molecular Biology of the Cell · doi:10.1091/mbc.e11-11-0958 · Cited by 52 · see citing works
Paddock, S., & Dunn, G. (1986) · Journal of Cell Science · doi:10.1242/jcs.81.1.163 · Cited by 47 · see citing works
Rapid Remodeling of Invadosomes by Gi-coupled Receptors
Kedziora, K. M., Leyton-Puig, D., Argenzio, E., Boumeester, A. J., van Butselaar, B., Yin, T., Wu, Y., van Leeuwen, F. N., Innocenti, M., Jalink, K., et al. (2016) · Journal of Biological Chemistry · doi:10.1074/jbc.m115.695940 · Cited by 38 · see citing works
Self-citationSwiercz, J. M., Worzfeld, T., & Offermanns, S. (2009) · Molecular and Cellular Biology · doi:10.1128/mcb.00103-09 · Cited by 35 · see citing works
Choudhary, S., Blackwell, K. A., Voznesensky, O. S., Roy, A. D., & Pilbeam, C. C. (2013) · Bone · doi:10.1016/j.bone.2013.04.017 · Cited by 24 · see citing works
Self-citationDisplacement and translocation of osteoblast-like cells by osteoclasts
Ferrier, J., Xia, S.-L., Lagan, E., Aubin, J. E., & Heersche, J. N. M. (1994) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.5650090911 · Cited by 21 · see citing works
Flagged first, then by citations
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Sorted by citations
Zhou, X., Wahane, S., Friedl, M.-S., Kluge, M., Friedel, C. C., Avrampou, K., Zachariou, V., Guo, L., Zhang, B., He, X., et al. (2020) · Nature Neuroscience · doi:10.1038/s41593-020-0597-7 · Cited by 275 · see citing works
Glucocorticoids and bone: consequences of endogenous and exogenous excess and replacement therapy
Hardy, R. S., Zhou, H., Seibel, M. J., & Cooper, M. S. (2018) · Endocrine Reviews · doi:10.1210/er.2018-00097 · Cited by 258 · see citing works
Crosstalk between Bone and Nerves within Bone
Wan, Q., Qin, W., Ma, Y., Shen, M., Li, J., Zhang, Z., Chen, J., Tay, F. R., Niu, L., & Jiao, K. (2021) · Advanced Science · doi:10.1002/advs.202003390 · Cited by 212 · see citing works
Wang, N., Liu, X., Tang, Z., Wei, X., Dong, H., Liu, Y., Wu, H., Wu, Z., Li, X., Ma, X., et al. (2022) · Journal of Nanobiotechnology · doi:10.1186/s12951-022-01267-2 · Cited by 74 · see citing works
Emerging roles of nerve‐bone axis in modulating skeletal system
Li, J., Zhang, Z., Tang, J., Hou, Z., Li, L., & Li, B. (2024) · Medicinal Research Reviews · doi:10.1002/med.22031 · Cited by 67 · see citing works
The Role of Semaphorin 4D in Bone Remodeling and Cancer Metastasis
Lontos, K., Adamik, J., Tsagianni, A., Galson, D. L., Chirgwin, J. M., & Suvannasankha, A. (2018) · Frontiers in Endocrinology · doi:10.3389/fendo.2018.00322 · Cited by 64 · see citing works
Crosstalk between skeletal and neural tissues is critical for skeletal health
Abeynayake, N., Arthur, A., & Gronthos, S. (2020) · Bone · doi:10.1016/j.bone.2020.115645 · Cited by 64 · see citing works
Li, Y., Kang, S., Halawani, D., Wang, Y., Alves, C. J., Ramakrishnan, A., Estill, M. S., Shen, L., Li, F., He, X., et al. (2022) · Genes & Development · doi:10.1101/gad.349063.121 · Cited by 53 · see citing works
Role of Slit/Robo Signaling pathway in Bone Metabolism
Jiang, L., Sun, J., & Huang, D. (2022) · International Journal of Biological Sciences · doi:10.7150/ijbs.66931 · Cited by 51 · see citing works
Ligand-independent receptor clustering modulates transmembrane signaling: a new paradigm
Sánchez, M. F., & Tampé, R. (2022) · Trends in Biochemical Sciences · doi:10.1016/j.tibs.2022.08.002 · Cited by 47 · see citing works
Stedden, C. G., Menegas, W., Zajac, A. L., Williams, A. M., Cheng, S., Özkan, E., & Horne‐Badovinac, S. (2019) · Current Biology · doi:10.1016/j.cub.2019.01.049 · Cited by 47 · see citing works
Biochemical activity of magnesium ions on human osteoblast migration
Choi, S., Kim, K., Cheon, S., Kim, E., Kim, Y.-A., Park, C., & Kim, K. K. (2020) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2020.07.057 · Cited by 44 · see citing works
SLPI is a critical mediator that controls PTH-induced bone formation
Morimoto, A., Kikuta, J., Nishikawa, K., Sudo, T., Uenaka, M., Furuya, M., Hasegawa, T., Hashimoto, K., Tsukazaki, H., Seno, S., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-22402-x · Cited by 44 · see citing works
Xie, J., Wang, Z., & Wang, W. (2020) · Frontiers in Immunology · doi:10.3389/fimmu.2020.02151 · Cited by 43 · see citing works
Huang, Y., Wang, M., Ni, H., Zhang, J., Li, A., Hu, B., Junqueira Alves, C., Wahane, S., Rios de Anda, M., Ho, L., et al. (2024) · Nature Neuroscience · doi:10.1038/s41593-024-01664-w · Cited by 42 · see citing works
“Plexin signaling is best known for growth cone collapse during neurodevelopment; recent studies from our laboratory and others have further unveiled a role of plexins in cell collision guidance 58,59 .”
Bone metabolism regulation: Implications for the treatment of bone diseases
Yao, D., Huang, L., Ke, J., Zhang, M., Xiao, Q., & Zhu, X. M. (2020) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2020.110494 · Cited by 38 · see citing works
Qian, D., Zhang, Q., He, C., Guo, J., Huang, X., Zhao, J., Zhang, H., Xu, C., & Peng, W. (2024) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2024.118234 · Cited by 25 · see citing works
Steering Molecular Activity with Optogenetics: Recent Advances and Perspectives
Oh, T., Fan, H., Skeeters, S. S., & Zhang, K. (2021) · Advanced Biology · doi:10.1002/adbi.202000180 · Cited by 24 · see citing works
Directing with restraint: Mechanisms of protrusion restriction in collective cell migrations
Roberto, G. M., & Emery, G. (2022) · Seminars in Cell and Developmental Biology · doi:10.1016/j.semcdb.2022.03.037 · Cited by 19 · see citing works
Crosstalk between axon guidance signaling and bone remodeling
Nakanishi, Y., Kang, S., & Kumanogoh, A. (2021) · Bone · doi:10.1016/j.bone.2021.116305 · Cited by 18 · see citing works
The emerging roles of semaphorin4D/CD100 in immunological diseases
Wang, L., Li, X., Song, Y., Song, D., & Huang, D. (2020) · Biochemical Society Transactions · doi:10.1042/bst20200821 · Cited by 16 · see citing works
Marie, P. J., & Cohen-Solal, M. E. (2017) · Journal of Bone and Mineral Research · doi:10.1002/jbmr.3356 · Cited by 15 · see citing works
Impact of Different Cell Types on the Osteogenic Differentiation Process of Mesenchymal Stem Cells
Wang, Z., Ren, L., Li, Z., Qingyuan, Q., Wang, H., Huang, X., & Ma, D. (2025) · Stem Cells International · doi:10.1155/sci/5551222 · Cited by 16 · see citing works
Xu, Q., Liu, H., Yu, B., Chen, W., Zhai, L., Li, X., & Fang, Y. (2019) · OncoTargets and Therapy · doi:10.2147/ott.s212749 · Cited by 12 · see citing works
Matsubayashi, H. T., Mountain, J., Takahashi, N., Roy, A. D., Yao, T., Peterson, A. F., Gonzalez, C. S., Kawamata, I., & Inoue, T. (2024) · Nature Communications · doi:10.1038/s41467-024-46855-y · Cited by 10 · see citing works
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Nojima, S. (2022) · Pathology International · doi:10.1111/pin.13270 · Cited by 8 · see citing works
Xu, X., Li, X., Wang, Z., Yu, J., Li, C., Du, M., Guo, Q., Guo, Q., & Zhou, E. (2023) · Chemical Engineering Journal · doi:10.1016/j.cej.2023.147206 · Cited by 8 · see citing works
Ni, H., Zhou, Z., Estill, M. S., Halawani, D., Alves, C. J., Shen, L., Xie, N., Friedel, R. H., & Zou, H. (2025) · Nature Communications · doi:10.1038/s41467-025-65095-2 · Cited by 7 · see citing works
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Buck, M. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-819460-7.00345-5 · Cited by 7 · see citing works
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Ueda, Y., & Sato, M. (2018) · ChemBioChem · doi:10.1002/cbic.201700635 · Cited by 6 · see citing works
Takada, A., Asano, T., Nakahama, K., Ono, T., Nakata, T., & Ishii, T. (2024) · Scientific Reports · doi:10.1038/s41598-024-52056-w · Cited by 5 · see citing works
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Teng, X., Wang, P., Yang, T., Huang, W., Yu, H., Li, W., Chen, Z., & Fan, D. (2020) · Human & Experimental Toxicology · doi:10.1177/0960327120975125 · Cited by 3 · see citing works
Povoroznyuk, V. V., Dedukh, N. V., Bystrytska, M. А., & Shapovalov, V. S. (2021) · Regulatory Mechanisms in Biosystems · doi:10.15421/022130 · Cited by 4 · see citing works
Deb Roy, A., Saez Gonzalez, C., Shahid, F., Yadav, E., & Inoue, T. (2024) · doi:10.1101/2024.12.01.626286 · Cited by 4 · see citing works
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Deb Roy, A., Gonzalez, C. S., Stanislauskas, M., Shahid, F., Yadav, E., Rezek, J., & Inoue, T. (2026) · Journal of Cell Biology · doi:10.1083/jcb.202508095 · Cited by 2 · see citing works
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Endo, M., & Ozawa, T. (2021) · Advances in experimental medicine and biology · doi:10.1007/978-981-15-8763-4_15 · Cited by 2 · see citing works
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Roy, T. B., Heidari, M., & Dokholyan, N. V. (2025) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2025.103126 · Cited by 1 · see citing works
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Li, J., Ni, H., Halawani, D., Estill, M. S., Ramakrishnan, A., Alves, C. J., Shen, L., He, X., Friedel, R. H., & Zou, H. (2026) · Science Advances · doi:10.1126/sciadv.adw4136 · Cited by 1 · see citing works
Biological Events at the Interface Between the Radicular Part of a Dental Implant and Bone
Boyan, B. D., Lotz, E. M., Berger, M. B., Deng, J., Cohen, D. J., & Schwartz, Z. (2023) · Springer eBooks · doi:10.1007/978-3-031-37234-6_11 · Cited by 1 · see citing works
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Wang, N., Liu, X., Tang, Z., Wei, X., Dong, H., Liu, Y., Wu, H., Wu, Z., Li, X., Ma, X., et al. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.09.07.459238
Redondo, G. M., Malta, F. S., da Silva, V. S., & Bastos, M. F. (2021) · Colloquium health and education · doi:10.37497/colloquium.v1i2.12
Qualitative and asymptotic analysis of repulsion systems with diverse applications
Solís, F. J., Sánchez, F. S., & Gonzalez, L. M. (2024) · The Journal of Difference Equations and Applications · doi:10.1080/10236198.2024.2348514
Guiding Cells with Light and Chemicals: A Toolbox for Dynamic Studies of Cell MigrationPreprint
Roy, A. D., Rho, E., & Inoue, T. (2026) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a041754
Yang, Y., & Zhang, C. (2026) · Electronic Journal of Biotechnology · doi:10.1016/j.ejbt.2026.100712
- Authors
- Deb Roy, A., Yin, T., Choudhary, S., Rodionov, V., Pilbeam, C. C., & Wu, Y. I.
- Affiliations
- University of Connecticut (US)
- Corresponding author
- Wu, Yi I.
- Venue
- Nature Communications
- Published
- 2017-06-21
- Volume
- 8
- Issue
- 1
- Pages
- 15831-15831
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC5482063
- OpenAlex
- W2663175999
- Cited by
- 44
- References
- 70
- Citations by year
- 2026: 4 · 2025: 3 · 2024: 7 · 2023: 2 · 2022: 6 · 2021: 9 · 2020: 7 · 2019: 2 · 2018: 3 · 2017: 1
- FWCI
- 1.74
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Nature Communications (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Institute of General Medical Sciences (R01GM117061, R01GM062290)
- National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR060286)
- Topics
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Axon Guidance and Neuronal Signaling
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bone Metabolism and Diseases
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Keywords
- Plexin-B1, Sema4D, contact inhibition of locomotion, osteoclasts, osteoblasts, bone remodeling, cell migration, optogenetic stimulation, Cdc42, RhoA/ROCK signaling, betaPIX, myosin II, phosphatidylinositol 3,4,5-trisphosphate, cell protrusions, chemotaxis
- MeSH terms
- Animals, Cell Movement, Light, Male, Mice, Inbred C57BL, Nerve Tissue Proteins, Osteoblasts, Osteoclasts, Receptors, Cell Surface, Signal Transduction, Cell Polarity, rhoA GTP-Binding Protein, cdc42 GTP-Binding Protein, Myosin Type II, Semaphorins, Mice, Optogenetics
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