Manipulation of nuclear architecture through CRISPR-mediated chromosomal looping
Open accessAbstract
Chromatin looping is key to gene regulation, yet no broadly applicable methods to selectively modify chromatin loops have been described. We have engineered a method for chromatin loop reorganization using CRISPR-dCas9 (CLOuD9) to selectively and reversibly establish chromatin loops. We demonstrate the power of this technology to selectively modulate gene expression at targeted loci.
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Stimulation of promoter activity by forced looping with an enhancer is a strong argument in favor of the loop model.
“Stimulation of promoter activity by forced looping with an enhancer is a strong argument in favor of this model [87,88,110,111].”
From Enhancer Function in the 3D Genome (2023) · cites this paper as [110]
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Cites
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Self-citation
- Authors
- Morgan, S. L., Mariano, N. C., Bermudez, A., Arruda, N. L., Wu, F., Luo, Y., Shankar, G., Jia, L., Chen, H., Hu, J.-F., Hoffman, A. R., Huang, C.-C., Pitteri, S. J., & Wang, K. C.
- Affiliations
- Stanford Medicine (US)
- Bridgewater State University (US)
- System Biosciences (United States) (US)
- Jilin University (CN)
- Central South University (CN)
- VA Palo Alto Health Care System (US)
- ORCID
- Venue
- Nature Communications
- Published
- 2017-07-13
- Volume
- 8
- Issue
- 1
- Pages
- 15993-15993
- ISSN
- 2041-1723 (ISSN-L)
- PMC
- PMC5511349
- OpenAlex
- W2736242246
- Cited by
- 279
- References
- 3
- Citations by year
- 2026: 13 · 2025: 22 · 2024: 19 · 2023: 26 · 2022: 33 · 2021: 46 · 2020: 39 · 2019: 41 · 2018: 34 · 2017: 6
- FWCI
- 13.41
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Nature Communications (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Funders
- National Science Foundation
- U.S. Department of Veterans Affairs (I01 BX002905)
- Burroughs Wellcome Fund
- Donald E. and Delia B. Baxter Foundation
- National Defense Science and Engineering Graduate
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Chromatin Remodeling and Cancer
- Keywords
- chromatin looping, CRISPR/dCas9, nuclear organization, gene expression regulation, gene expression modulation
- MeSH terms
- Humans, Promoter Regions, Genetic, K562 Cells, Chromatin Assembly and Disassembly, DEAD-box RNA Helicases, beta-Globins, HEK293 Cells, CRISPR-Cas Systems
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