α-Tanycytes of the adult hypothalamic third ventricle include distinct populations of FGF-responsive neural progenitors
Open accessAbstract
Emerging evidence suggests that new cells, including neurons, can be generated within the adult hypothalamus, suggesting the existence of a local neural stem/progenitor cell niche. Here, we identify α-tanycytes as key components of a hypothalamic niche in the adult mouse. Long-term lineage tracing in vivo using a GLAST::CreERT2conditional driver indicates that α-tanycytes are self-renewing cells that constitutively give rise to new tanycytes, astrocytes and sparse numbers of neurons.In vitro studies demonstrate that α-tanycytes, but not β-tanycytes or parenchymal cells, are neurospherogenic. Distinct subpopulations of α-tanycytes exist, amongst which only GFAP-positive dorsal α2-tanycytes possess stem-like neurospherogenic activity.Fgf-10 and Fgf-18 are expressed specifically within ventral tanycyte subpopulations; α-tanycytes require fibroblast growth factor signalling to maintain their proliferation ex vivo and elevated fibroblast growth factor levels lead to enhanced proliferation of α-tanycytes in vivo.Our results suggest that α-tanycytes form the critical component of a hypothalamic stem cell niche, and that local fibroblast growth factor signalling governs their proliferation.
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This corroborates findings indicating the existence of neural stem cells in the OVLT, SFO, ME and AP.
“which corroborates findings indicating the existence of neural stem cells in the OVLT, SFO, ME and AP78–81.”
From A high-resolution transcriptomic and spatial atlas of cell types in the whole mouse brain (2023) · cites this paper as [81]
The Discussion (page unavailable) reports evidence of neural stem-cell potential in median eminence (ME) β2-tanycytes in postnatal or juvenile animals, while distinguishing that finding from this paper’s results in adults. The cited paper does not address neural stem cells in the OVLT, SFO or AP, so it supports only the ME portion of the attributed claim. (LLM, conf=0.87)
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Cites
Massagué, J. (1998) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.67.1.753 · Cited by 7,571 · see citing works
Reynolds, B. A., & Weiss, S. (1992) · Science · doi:10.1126/science.1553558 · Cited by 5,413 · see citing works
Generalized lacZ expression with the ROSA26 Cre reporter strain
Soriano, P. (1999) · Nature Genetics · doi:10.1038/5007 · Cited by 5,237 · see citing works
Subventricular zone astrocytes are neural stem cells in the adult mammalian brain
Doetsch, F., Caillé, I., Lim, D. A., García-Verdugo, J. M., & Alvarez-Buylla, A. (1999) · Cell · doi:10.1016/s0092-8674(00)80783-7 · Cited by 3,898 · see citing works
Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence
Bignami, A., Eng, L. F., Dahl, D. R., & Uyeda, C. T. (1972) · Brain Research · doi:10.1016/0006-8993(72)90398-8 · Cited by 1,456 · see citing works
Morshead, C. M., Reynolds, B. A., Craig, C. G., McBurney, M. W., Staines, W. A., Morassutti, D., Weiss, S., & van der Kooy, D. (1994) · Neuron · doi:10.1016/0896-6273(94)90046-9 · Cited by 1,403 · see citing works
Lois, C., & Álvarez-Buylla, A. (1993) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.90.5.2074 · Cited by 1,339 · see citing works
Multipotent CNS Stem Cells Are Present in the Adult Mammalian Spinal Cord and Ventricular Neuroaxis
Weiss, S., Dunne, C., Hewson, J., Wohl, C. A., Wheatley, M., Peterson, A. C., & Reynolds, B. A. (1996) · Journal of Neuroscience · doi:10.1523/jneurosci.16-23-07599.1996 · Cited by 1,219 · see citing works
Shen, Q., Wang, Y., Kokovay, E., Lin, G., Chuang, S.-M., Goderie, S. K., Roysam, B., & Temple, S. (2008) · Cell stem cell · doi:10.1016/j.stem.2008.07.026 · Cited by 1,027 · see citing works
Mirzadeh, Z., Merkle, F. T., Soriano-Navarro, M., Garcia-Verdugo, J. M., & Alvarez-Buylla, A. (2008) · Cell stem cell · doi:10.1016/j.stem.2008.07.004 · Cited by 1,015 · see citing works
Radial glia give rise to adult neural stem cells in the subventricular zone
Merkle, F. T., Tramontin, A. D., García‐Verdugo, J. M., & Álvarez-Buylla, A. (2004) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0407893101 · Cited by 849 · see citing works
Neurogenesis in the Hypothalamus of Adult Mice: Potential Role in Energy Balance
Kokoeva, M. V., Yin, H., & Flier, J. S. (2005) · Science · doi:10.1126/science.1115360 · Cited by 704 · see citing works
Self-citationZ/AP, a Double Reporter for Cre-Mediated Recombination
Lobe, C. G., Koop, K. E., Kreppner, W., Lomelı́, H., Gertsenstein, M., & Nagy, A. (1999) · Developmental Biology · doi:10.1006/dbio.1999.9209 · Cited by 528 · see citing works
Tanycytes of the hypothalamic median eminence form a diet-responsive neurogenic niche
Lee, D. A., Bedont, J. L., Pak, T., Wang, H., Song, J., Miranda-Angulo, A., Takiar, V., Charubhumi, V., Balordi, F., Takebayashi, H., et al. (2012) · Nature Neuroscience · doi:10.1038/nn.3079 · Cited by 502 · see citing works
Hypothalamic tanycytes: a key component of brain-endocrine interaction
RODRIGUEZ, E., BLAZQUEZ, J., PASTOR, F., PELAEZ, B., PENA, P., PERUZZO, B., & AMAT, P. (2005) · International review of cytology · doi:10.1016/s0074-7696(05)47003-5 · Cited by 475 · see citing works
Virchow, V. R. (1861) · The American Journal of the Medical Sciences · doi:10.1097/00000441-186104000-00025 · Cited by 402 · see citing works
Mori, T., Tanaka, K., Buffo, A., Wurst, W., Kühn, R., & Götz, M. (2006) · Glia · doi:10.1002/glia.20350 · Cited by 398 · see citing works
Li, J., Tang, Y., & Cai, D. (2012) · Nature Cell Biology · doi:10.1038/ncb2562 · Cited by 344 · see citing works
Neurogenesis in the ependymal layer of the adult rat 3rd ventricle
Xu, Y., Tamamaki, N., Noda, T., Kimura, K., Itokazu, Y., Matsumoto, N., Dezawa, M., & Idé, C. (2005) · Experimental Neurology · doi:10.1016/j.expneurol.2004.12.021 · Cited by 331 · see citing works
Werner, S., Duan, D., de Vries, C. D., Peters, K., Johnson, D. E., & Williams, L. (1992) · Molecular and Cellular Biology · doi:10.1128/mcb.12.1.82-88.1992 · Cited by 331 · see citing works
Remodeling of the arcuate nucleus energy-balance circuit is inhibited in obese mice
McNay, D. E. G., Briançon, N., Kokoeva, M. V., Maratos–Flier, E., & Flier, J. S. (2011) · Journal of Clinical Investigation · doi:10.1172/jci43134 · Cited by 312 · see citing works
Self-citationHypothalamic tanycytes: potential roles in the control of feeding and energy balance
Bolborea, M., & Dale, N. (2013) · Trends in Neurosciences · doi:10.1016/j.tins.2012.12.008 · Cited by 301 · see citing works
Notch1 is required for neuronal and glial differentiation in the cerebellum
Lütolf, S., Radtke, F., Aguet, M., Suter, U., & Taylor, V. (2002) · Development · doi:10.1242/dev.129.2.373 · Cited by 264 · see citing works
Self-citationFriedman, B., Hockfield, S. J., Black, J. A., Woodruff, K., & Waxman, S. G. (1989) · Glia · doi:10.1002/glia.440020510 · Cited by 248 · see citing works
Evidence for constitutive neural cell proliferation in the adult murine hypothalamus
Kokoeva, M. V., Yin, H., & Flier, J. S. (2007) · The Journal of Comparative Neurology · doi:10.1002/cne.21492 · Cited by 246 · see citing works
Self-citationHaan, N., Goodman, T. H., Najdi-Samiei, A., Stratford, C. M., Rice, R., Agha, E. E., Bellusci, S., & Hajihosseini, M. K. (2013) · Journal of Neuroscience · doi:10.1523/jneurosci.2437-12.2013 · Cited by 239 · see citing works
Distinct Modes of Neuron Addition in Adult Mouse Neurogenesis
Ninkovic, J., Mori, T., & Götz, M. (2007) · The Journal of Neuroscience · doi:10.1523/jneurosci.2572-07.2007 · Cited by 235 · see citing works
Self-citationDe Novo Neurogenesis in Adult Hypothalamus as a Compensatory Mechanism to Regulate Energy Balance
Pierce, A. A., & Xu, A. W. (2010) · Journal of Neuroscience · doi:10.1523/jneurosci.2479-09.2010 · Cited by 224 · see citing works
Structural basis by which alternative splicing modulates the organizer activity of FGF8 in the brainPreprint
Olsen, S. K., Li, J. Y. H., Bromleigh, C., Eliseenkova, A. V., Ibrahimi, O. A., Lao, Z., Zhang, F., Linhardt, R. J., Joyner, A. L., & Mohammadi, M. (2005) · Genes & Development · doi:10.1101/gad.1365406 · Cited by 204 · see citing works
Microtubule-associated proteins: a monoclonal antibody to MAP2 binds to differentiated neurons.
Izant, J. G., & McIntosh, J. R. (1980) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.77.8.4741 · Cited by 203 · see citing works
Migaud, M., Batailler, M., Segura, S., Duittoz, A. H., Franceschini, I., & Pillon, D. (2010) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.2010.07521.x · Cited by 194 · see citing works
Zheng, W., Nowakowski, R. S., & Vaccarino, F. M. (2004) · Developmental Neuroscience · doi:10.1159/000082136 · Cited by 191 · see citing works
IGF‐I stimulates neurogenesis in the hypothalamus of adult rats
Pérez‐Martín, M., Cifuentes, M., Grondona, J. M., López-Ávalos, M. D., Gómez‐Pinedo, U., García‐Verdugo, J. M., & Fernández‐Llebrez, P. (2010) · European Journal of Neuroscience · doi:10.1111/j.1460-9568.2010.07220.x · Cited by 173 · see citing works
The FGF-2/FGFRs neurotrophic system promotes neurogenesis in the adult brain
Mudò, G., Bonomo, A., Di Liberto, V., Frinchi, M., Fuxe, K., & Belluardo, N. (2009) · Journal of Neural Transmission · doi:10.1007/s00702-009-0207-z · Cited by 158 · see citing works
Endogenous and exogenous ciliary neurotrophic factor enhances forebrain neurogenesis in adult mice
Emsley, J. G. (2003) · Experimental Neurology · doi:10.1016/s0014-4886(03)00129-8 · Cited by 158 · see citing works
Novel Neuronal Phenotypes from Neural Progenitor Cells
Markakis, E. A., Palmer, T. D., Randolph‐Moore, L., RAKIC, P. T., & Gage, F. H. (2004) · Journal of Neuroscience · doi:10.1523/jneurosci.4161-03.2004 · Cited by 149 · see citing works
A Golgi study of third ventricle tanycytes in the adult rodent brain
Millhouse, O. E. (1971) · Cell and Tissue Research · doi:10.1007/bf00330913 · Cited by 120 · see citing works
Prévot, V., Bellefontaine, N., Baroncini, M., Sharif, A., Hanchate, N. K., Parkash, J., Campagne, C., & Seranno, S. D. (2010) · Journal of Neuroendocrinology · doi:10.1111/j.1365-2826.2010.02033.x · Cited by 105 · see citing works
Altman, J., & Bayer, S. A. (1978) · The Journal of Comparative Neurology · doi:10.1002/cne.901820513 · Cited by 99 · see citing works
Caccamo, D. V., Katsetos, C. D., Herman, M. M., Frankfurter, A. J., Collins, V. P., & Rubinstein, L. J. (1989) · PubMed · Cited by 97 · see citing works
The relations of ependyma to neurons and capillaries in the hypothalamus: A Golgi‐Cox study
Bleier, R. (1971) · The Journal of Comparative Neurology · doi:10.1002/cne.901420404 · Cited by 89 · see citing works
Wnt Signaling Regulates Postembryonic Hypothalamic Progenitor Differentiation
Wang, X., Kopinke, D., Lin, J., McPherson, A. D., Duncan, R. N., Otsuna, H., Moro, E., Hoshijima, K., Grunwald, D. J., Argenton, F., et al. (2012) · Developmental Cell · doi:10.1016/j.devcel.2012.07.012 · Cited by 87 · see citing works
Morphological aspects of the hypothalamic-hypophyseal system
Akmayev, I. G., Фиделина, О. В., Kabolova, Z. A., Popov, A. P., & Schitkova, T. A. (1973) · Cell and Tissue Research · doi:10.1007/bf00307226 · Cited by 82 · see citing works
The fine structure of the ependymal tanycytes at the level of the arcuate nucleus
Brawer, J. R. (1972) · The Journal of Comparative Neurology · doi:10.1002/cne.901450103 · Cited by 74 · see citing works
Morphological aspects of the hypothalamic-hypophyseal system
Akmayev, I. G. (1969) · Cell and Tissue Research · doi:10.1007/bf00973337 · Cited by 57 · see citing works
Lechan, R. M., & Fekete, C. (2007) · Acta bio-medica dell'Ateneo parmense/Acta biomedica · Cited by 51 · see citing works
Hajihosseini, M. K., Langhe, S. D., Lana‐Elola, E., et al. (2008) · Molecular and Cellular Neuroscience · doi:10.1016/j.mcn.2008.01.008 · Cited by 51 · see citing works
Morphological aspects of the hypothalamic-hypophyseal system
Akmayev, I. G., & Фиделина, О. В. (1976) · Cell and Tissue Research · doi:10.1007/bf00220328 · Cited by 48 · see citing works
Scanning and Transmission Electron Microscopy of the Ependymal Lining of the Third Ventricle
Bruni, J. (1974) · Canadian Journal of Neurological Sciences / Journal Canadien des Sciences Neurologiques · doi:10.1017/s0317167100019570 · Cited by 39 · see citing works
Ultrastructure of supraependymal cells and ependyma of hypothalamic third ventricle of mouse
Bleier, R. (1977) · The Journal of Comparative Neurology · doi:10.1002/cne.901740211 · Cited by 34 · see citing works
Role of Fibroblast Growth Factor Receptors in Astrocytic Stem Cells
Gálvez‐Contreras, A. Y., González-Castañeda, R. E., Luquı́n, S., & González-Pérez, Ó. (2012) · Current Signal Transduction Therapy · doi:10.2174/157436212799278205 · Cited by 26 · see citing works
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A high-resolution transcriptomic and spatial atlas of cell types in the whole mouse brain
Yao, Z., van Velthoven, C. T. J., Kunst, M., Zhang, M., McMillen, D., Lee, C., Jung, W., Goldy, J., Abdelhak, A., Aitken, M., et al. (2023) · Nature · doi:10.1038/s41586-023-06812-z · Cited by 1,194 · see citing works
Single-Cell RNA-Seq Reveals Hypothalamic Cell Diversity
Chen, R., Wu, X., Jiang, L., & Zhang, Y. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.03.004 · Cited by 592 · see citing works
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Fiorelli, R., Azim, K., Fischer, B., & Raineteau, O. (2015) · Development · doi:10.1242/dev.119966 · Cited by 116 · see citing works
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Kiecker, C. (2018) · Journal of Anatomy · doi:10.1111/joa.12771 · Cited by 92 · see citing works
Morita, S., Furube, E., Mannari, T., Okuda, H., Tatsumi, K., Wanaka, A., & Miyata, S. (2016) · Cell and Tissue Research · doi:10.1007/s00441-015-2207-7 · Cited by 88 · see citing works
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Miyata, S. (2022) · Frontiers in Neuroscience · doi:10.3389/fnins.2022.991779 · Cited by 66 · see citing works
Furube, E., Morita, M., & Miyata, S. (2015) · Cell and Tissue Research · doi:10.1007/s00441-015-2201-0 · Cited by 47 · see citing works
de Vries, E. M., Nagel, S., Haenold, R., Sundaram, S. M., Pfrieger, F. W., Fliers, E., Heuer, H., & Boelen, A. (2016) · Endocrinology · doi:10.1210/en.2016-1027 · Cited by 23 · see citing works
- Authors
- Robins, S. C., Stewart, I., McNay, D. E., Taylor, V., Giachino, C., Goetz, M., Ninkovic, J., Briancon, N., Maratos-Flier, E., Flier, J. S., Kokoeva, M. V., & Placzek, M.
- Affiliations
- University of Sheffield (GB)
- Beth Israel Deaconess Medical Center (US)
- University of Basel (CH)
- Helmholtz Munich (DE)
- Corresponding author
- Placzek, M.
- Venue
- Nature Communications
- Published
- 2013-06-27
- Volume
- 4
- Issue
- 1
- Pages
- 2049-2049
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2080692887
- Cited by
- 306
- References
- 52
- Citations by year
- 2026: 8 · 2025: 13 · 2024: 12 · 2023: 26 · 2022: 17 · 2021: 32 · 2020: 36 · 2019: 15 · 2018: 27 · 2017: 37 · 2016: 25 · 2015: 29 · 2014: 20 · 2013: 9
- FWCI
- 10.27
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Nature Communications (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Repository@Nottingham (University of Nottingham) (publishedVersion, other-oa)
- White Rose Research Online (University of Leeds, The University of Sheffield, University of York) (acceptedVersion)
- Repository@Nottingham (University of Nottingham) (submittedVersion, cc-by)
- RePEc: Research Papers in Economics (submittedVersion)
- Aberdeen University Research Archive (Aberdeen University) (submittedVersion)
- Site cant be reached (submittedVersion)
- Funders
- Deutsche Forschungsgemeinschaft (SPP1109, SPP1109; TA-310)
- Medical Research Council
- Biotechnology and Biological Sciences Research Council
- National Institute of Diabetes and Digestive and Kidney Diseases (P01DK56116, P01DK056116, DK-R37-28082, R37DK028082)
- Topics
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Regulation of Appetite and Obesity
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Keywords
- hypothalamus, third ventricle, neural stem/progenitor cells, adult neurogenesis, FGF signaling, FGF10, FGF18, astrocyte differentiation, lineage tracing, cell proliferation
- MeSH terms
- Aging, Animals, Epidermal Growth Factor, Fibroblast Growth Factors, Glial Fibrillary Acidic Protein, Hypothalamus, Immunohistochemistry, Mice, Inbred C57BL, Neuroglia, Signal Transduction, Spheroids, Cellular, Integrases, Third Ventricle, Excitatory Amino Acid Transporter 1, Cell Proliferation, Mice, Fibroblast Growth Factor 10, Neural Stem Cells, Ependymoglial Cells
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