Phosphorylation of BLUS1 kinase by phototropins is a primary step in stomatal opening
Open accessAbstract
Opening of stomata in the plant facilitates photosynthetic CO2 fixation and transpiration. Blue-light perception by phototropins (phot1, phot2) activates the plasma membrane H+-ATPase, causing stomata to open. Here we describe a regulator that connects these components, a Ser/Thr protein kinase, BLUS1 (BLU E LIGHT S IGNALING 1), which mediates a primary step for phototropin signalling in guard cells. blus1 mutants identified by infrared thermography result in a loss of blue light-dependent stomatal opening. BLUS1 encodes a protein kinase that is directly phosphorylated by phot1 in vitro and in vivo at Ser-348 within its C-terminus. Both phosphorylation of Ser-348 and BLUS1 kinase activity are essential for activation of the H+-ATPase. blus1 mutants show lower stomatal conductance and CO2 assimilation than wild-type plants under decreased ambient CO2. Together, our analyses demonstrate that BLUS1 functions as a phototropin substrate and primary regulator of stomatal control to enhance photosynthetic CO2 assimilation under natural light conditions. Blue light stimulates stomatal opening in Arabidopsis by activating phototropins. By screening for mutants that lack this response, the authors isolate the protein kinase blus1, and show that its phosphorylation by phototropin-1 promotes guard cell signalling and stomatal opening.
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Stomatal opening is regulated by the functional redundancy of PHOT1 and PHOT2.
“Stomatal opening is regulated by the functional redundancy of PHOT1 and PHOT2”
From The action of enhancing weak light capture via phototropic growth and chloroplast movement in plants (2022) · cites this paper as Takemiya et al., 2013
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“Under low fluence blue light circumstances, photoreceptors PHOT1 and PHOT2 mediate accumulation responses via RPT2, NCH1/ PI3K activation.”
BackgroundInfluentialSuetsugu, N., & Wada, M. (2017) · International Journal of Molecular Sciences · doi:10.3390/ijms18071469 · Cited by 7 · see citing works
- Authors
- Takemiya, A., Sugiyama, N., Fujimoto, H., Tsutsumi, T., Yamauchi, S., Hiyama, A., Tada, Y., Christie, J. M., & Shimazaki, K.-I.
- Affiliations
- Kyushu University (JP)
- Kyoto University (JP)
- Kagawa University (JP)
- University of Glasgow (GB)
- Corresponding author
- Shimazaki, Ken-ichiro
- ORCID
- Venue
- Nature Communications
- Published
- 2013-06-28
- Volume
- 4
- Issue
- 1
- Pages
- 2094-2094
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W1982064178
- Cited by
- 219
- References
- 42
- Citations by year
- 2026: 18 · 2025: 26 · 2024: 21 · 2023: 14 · 2022: 18 · 2021: 30 · 2020: 16 · 2019: 11 · 2018: 10 · 2017: 11 · 2016: 16 · 2015: 13 · 2014: 12 · 2013: 3
- FWCI
- 17.67
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Nature Communications (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- RePEc: Research Papers in Economics (submittedVersion)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (submittedVersion)
- Funders
- Sumitomo Foundation
- Ministry of Education, Culture, Sports, Science and Technology (21227001, 23120521)
- Research Foundation for Opto-Science and Technology
- Japan Society for the Promotion of Science (25120719, 13J05118, 23120521, 21227001)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- phototropin, phot1, phot2, stomatal opening, blue light signaling, protein phosphorylation, serine/threonine kinase, plasma membrane H+-ATPase, guard cells, stomatal conductance, carbon assimilation, Arabidopsis thaliana, infrared thermography
- MeSH terms
- Amino Acid Sequence, Carbon Dioxide, Cloning, Molecular, Light, Molecular Sequence Data, Mutation, Phosphorylation, Phosphoserine, Temperature, Genes, Plant, Arabidopsis, Arabidopsis Proteins, Plant Stomata, Phototropins, Carbon Cycle
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