The DOI matches its Crossref record; 9 of the 9 references it cites were found in a registry or an index.
Quantitative super-resolution imaging of Bruchpilot distinguishes active zone states
Open accessAbstract
The precise molecular architecture of synaptic active zones (AZs) gives rise to different structural and functional AZ states that fundamentally shape chemical neurotransmission. However, elucidating the nanoscopic protein arrangement at AZs is impeded by the diffraction-limited resolution of conventional light microscopy. Here we introduce new approaches to quantify endogenous protein organization at single-molecule resolution in situ with super-resolution imaging by direct stochastic optical reconstruction microscopy ( d STORM). Focusing on the Drosophila neuromuscular junction (NMJ), we find that the AZ cytomatrix (CAZ) is composed of units containing ~137 Bruchpilot (Brp) proteins, three quarters of which are organized into about 15 heptameric clusters. We test for a quantitative relationship between CAZ ultrastructure and neurotransmitter release properties by engaging Drosophila mutants and electrophysiology. Our results indicate that the precise nanoscopic organization of Brp distinguishes different physiological AZ states and link functional diversification to a heretofore unrecognized neuronal gradient of the CAZ ultrastructure.
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Cites
Molecular anatomy of a trafficking organelle
Takamori, S., Holt, M., Stenius, K., Lemke, E. A., Grønborg, M., Riedel, D., Urlaub, H., Schenck, S., Brügger, B., Ringler, P., et al. (2006) · Cell · doi:10.1016/j.cell.2006.10.030 · Cited by 2,373
Südhof, T. C. (2012) · Neuron · doi:10.1016/j.neuron.2012.06.012 · Cited by 1,062
Wagh, D. A., Rasse, T. M., Asan, E., Hofbauer, A., Schwenkert, I., Dürrbeck, H., Buchner, S., Dabauvalle, M.-C., Schmidt, M., Qin, G., et al. (2006) · Neuron · doi:10.1016/j.neuron.2006.02.008 · Cited by 908
Self-citationBruchpilot Promotes Active Zone Assembly, Ca 2+ Channel Clustering, and Vesicle Release
Kittel, R. J., Wichmann, C., Rasse, T. M., Fouquet, W., Schmidt, M., Schmid, A., Wagh, D. A., Pawlu, C., Kellner, R. R., Willig, K. I., et al. (2006) · Science · doi:10.1126/science.1126308 · Cited by 772
Self-citationRelease probability of hippocampal glutamatergic terminals scales with the size of the active zone
Holderith, N., Lorincz, A., Katona, G., Rózsa, B., Kulik, A., Watanabe, M., & Nusser, Z. (2012) · Nature Neuroscience · doi:10.1038/nn.3137 · Cited by 405
Widespread Changes in Synaptic Markers as a Function of Sleep and Wakefulness in Drosophila
Gilestro, G. F., Tononi, G., & Cirelli, C. (2009) · Science · doi:10.1126/science.1166673 · Cited by 360
Calcium Dependence of Exo- and Endocytotic Coupling at a Glutamatergic Synapse
Hosoi, N., Holt, M., & Sakaba, T. (2009) · Neuron · doi:10.1016/j.neuron.2009.06.010 · Cited by 256
Bassoon speeds vesicle reloading at a central excitatory synapse
Hallermann, S., Fejtova, A., Schmidt, H., Weyhersmüller, A., Silver, R. A., Gundelfinger, E. D., & Eilers, J. (2010) · Neuron · doi:10.1016/j.neuron.2010.10.026 · Cited by 223
Self-citationDetermination of absolute protein numbers in single synapses by a GFP-based calibration technique
Sugiyama, Y., Kawabata, I., Sobue, K., & Okabe, S. (2005) · Nature Methods · doi:10.1038/nmeth783 · Cited by 189
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Human autoantibodies to amphiphysin induce defective presynaptic vesicle dynamics and composition
Werner, C., Pauli, M., Doose, S., Weishaupt, A., Haselmann, H., Grünewald, B., Sauer, M., Heckmann, M., Toyka, K. V., Asan, E., et al. (2016) · Brain · doi:10.1093/brain/awv324 · Cited by 75
Self-citationThe Cerebellar Mossy Fiber Synapse as a Model for High-Frequency Transmission in the Mammalian CNS
Delvendahl, I., & Hallermann, S. (2016) · Trends in Neurosciences · doi:10.1016/j.tins.2016.09.006 · Cited by 52
Self-citationHomeostatic synaptic plasticity rescues neural coding reliability
Rozenfeld, E., Ehmann, N., Manoim, J. E., Kittel, R. J., & Parnas, M. (2023) · Nature Communications · doi:10.1038/s41467-023-38575-6 · Cited by 19
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Metadata
- Authors
- Ehmann, N., van de Linde, S., Alon, A., Ljaschenko, D., Keung, X. Z., Holm, T., Rings, A., DiAntonio, A., Hallermann, S., Ashery, U., Heckmann, M., Sauer, M., & Kittel, R. J.
- Venue
- Nature Communications
- Published
- 2014-08-18
- Volume
- 5
- Issue
- 1
- Pages
- 4650-4650
- ISSN
- 2041-1723 (ISSN-L)
- OpenAlex
- W2014786857
- Cited by
- 232
- References
- 9
- FWCI
- 9.66
- Type
- journal article
- Language
- en
- Open access
- hybrid
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
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