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Redox-responsive branched-bottlebrush polymers for in vivo MRI and fluorescence imaging

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Sowers, Molly A., McCombs, Jessica R., Wang, Ying, Paletta, Joseph T., Morton, Stephen W., Dreaden, Erik C., Boska, Michael D., Ottaviani, M. Francesca, Hammond, Paula T., Rajca, Andrzej, Johnson, Jeremiah A.·2014·Nature Communications

DOI 10.1038/ncomms6460

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Abstract

Stimuli-responsive multimodality imaging agents have broad potential in medical diagnostics. Herein, we report the development of a new class of branched-bottlebrush polymer dual-modality organic radical contrast agents—ORCAFluors—for combined magnetic resonance and near-infrared fluorescence imaging in vivo. These nitroxide radical-based nanostructures have longitudinal and transverse relaxation times that are on par with commonly used heavy-metal-based magnetic resonance imaging (MRI) contrast agents. Furthermore, these materials display a unique compensatory redox response: fluorescence is partially quenched by surrounding nitroxides in the native state; exposure to ascorbate or ascorbate/glutathione leads to nitroxide reduction and a concomitant 2- to 3.5-fold increase in fluorescence emission. This behaviour enables correlation of MRI contrast, fluorescence intensity and spin concentration with tissues known to possess high concentrations of ascorbate in mice. Our in vitro and in vivo results, along with our modular synthetic approach, make ORCAFluors a promising new platform for multimodality molecular imaging. Multimodal and stimuli-responsive imaging agents can yield more biological information than more typical single-mode or inert imaging probes. Here, the authors have made a dual-modal MRI-fluorescence probe and demonstrate its ability to image redox status in vivo.

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Authors: Sowers, M. A., McCombs, J. R., Wang, Y., Paletta, J. T., Morton, S. W., Dreaden, E. C., Boska, M. D., Ottaviani, M. F., Hammond, P. T., Rajca, A., & Johnson, J. A.
Venue: Nature Communications
Publisher: Springer Science and Business Media LLC
Published: 2014-11-18
Volume: 5
Issue: 1
Pages: 5460-5460
ISSN: 2041-1723 (ISSN-L)
DOI: 10.1038/ncomms6460
OpenAlex: W1977096237
Cited by: 279
References: 52
FWCI: 9.98
Type: journal article
Language: en
Open access: bronze

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Redox-responsive branched-bottlebrush polymers for in vivo MRI and fluorescence imaging

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