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MicroRNAs can generate thresholds in target gene expression
Open accessAbstract
MicroRNAs (miRNAs) are short, highly conserved noncoding RNA molecules that repress gene expression in a sequence-dependent manner. We performed single-cell measurements using quantitative fluorescence microscopy and flow cytometry to monitor a target gene's protein expression in the presence and absence of regulation by miRNA. We find that although the average level of repression is modest, in agreement with previous population-based measurements, the repression among individual cells varies dramatically. In particular, we show that regulation by miRNAs establishes a threshold level of target mRNA below which protein production is highly repressed. Near this threshold, protein expression responds sensitively to target mRNA input, consistent with a mathematical model of molecular titration. These results show that miRNAs can act both as a switch and as a fine-tuner of gene expression.
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Metadata
- Authors
- Mukherji, S., Ebert, M. S., Zheng, G. X. Y., Tsang, J. S., Sharp, P. A., & van Oudenaarden, A.
- Venue
- Nature Genetics
- Published
- 2011-08-21
- Volume
- 43
- Issue
- 9
- Pages
- 854-859
- ISSN
- 1061-4036 (ISSN-L)
- 1546-1718
- OpenAlex
- W2143145944
- Cited by
- 632
- References
- 28
- FWCI
- 19.82
- Type
- journal article
- Language
- en
- Open access
- green
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