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The DOI matches its Crossref record; 8 of the 8 references it cites were found in a registry or an index.

Reconstitution of human telomerase with the template RNA component hTR and the catalytic protein subunit hTRT

Weinrich, Scott L., Pruzan, Ron, Ma, Libin, Ouellette, Michel, Tesmer, Valeric M., Holt, Shawn E., Bodnar, Andrea G., Lichtsteiner, Serge, Kim, Nam W., Trager, James B., Taylor, Rebecca D., Carlos, Ruben, Andrews, William H., Wright, Woodring E., Shay, Jerry W., Harley, Calvin B., Morin, Gregg B.·1997·Nature Genetics
DOI 10.1038/ng1297-498
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Abstract

The maintenance of chromosome termini, or telomeres, requires the action of the enzyme telomerase, as conventional DNA polymerases cannot fully replicate the ends of linear molecules. Telomerase is expressed and telomere length is maintained in human germ cells and the great majority of primary human tumours. However, telomerase is not detectable in most normal somatic cells; this corresponds to the gradual telomere loss observed with each cell division. It has been proposed that telomere erosion eventually signals entry into senescence or cell crisis and that activation of telomerase is usually required for immortal cell proliferation. In addition to the human telomerase RNA component (hTR; ref. 11), TR1/TLP1 (refs 12, 13), a protein that is homologous to the p80 protein associated with the Tetrahymena enzyme, has been identified in humans. More recently, the human telomerase reverse transcriptase (hTRT; refs 15, 16), which is homologous to the reverse transcriptase (RT)-like proteins associated with the Euplotes aediculatus (Ea_p123), Saccharomyces cerevisiae (Est2p) and Schizosaccharomyces pombe (5pTrt1) telomerases, has been reported to be a telomerase protein subunit. A catalytic function has been demonstrated for Est2p in the RT-like class but not for p80 or its homologues. We now report that in vitro transcription and translation of hTRT when co-synthesized or mixed with hTR reconstitutes telomerase activity that exhibits enzymatic properties like those of the native e…

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Crossref · 10.1038/ng1297-498

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Research works printed but not linked: 23

  1. Olovnikov, A.M. A theory of marginotomy: the incomplete copying of template margin in enzymic synthesis of pronucleotides and biological significance of the phenomenon. J. Theor. Biol. 41, 181–190 (1973). · DOI
  2. Blackburn, E.H. Structure and function of telomeres. Nature 350, 569–573 (1991). · DOI · Paper page
  3. Lingner, J., Cooper, J.P. & Cech, T.R. Telomerase and DNA replication: no longer a lagging strand problem? Science 269, 1533–1534 (1995). · DOI
  4. Harley, C.B. Telomere loss: mitotic clock or genetic time bomb? Mutat. Res. 256, 271–282 (1991). · DOI · Paper page
  5. Harrington, L. et al. A mammalian telomerase-associated protein. Science 274, 973–977 (1997). · DOI
  6. Nakayama, J., Saito, M., Nakamura, H., Matsuura, A. & Ishikawa, F. TLP1: a gene encoding a protein component of mammalian telomerase is a novel member of WD repeats family. Cell 88, 1–20 (1997). · DOI
  7. Collins, K., Kobayashi, R. & Greider, C.W. Purification of Tetrahymena telomerase and cloning of genes encoding the two protein components of the enzyme. Cell 81, 677–686 (1995). · DOI
  8. Nakamura, T.M. et al. Telomerase catalytic subunit homologs from fission yeast and human. Science 277, 955–959 (1997). · DOI · Paper page
  9. Meyerson, M.I. et al. hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Cell 90, 785–795 (1997). · DOI · Paper page
  10. Lingner, J., Hughes, T.R., Shevchenko, A., Mann, M., Lundblad, V. & Cech, T.R. Reverse transcriptase motifs in the catalytic subunit of telomerase. Science 276, 561–567 (1997). · DOI · Paper page
  11. Counter, C.M., Meyerson, M., Eaton, E.N. & Weinberg, R.A. A catalytic subunit of yeast telomerase. Proc. Watl. Acad. Sci. USA 94, 9202–9207 (1997). · DOI
  12. Morin, G.B. The implications of telomerase biochemistry for human disease. Eur. J. Cancer 33, 750–760 (1997). · DOI
  13. Morin, G.B. Recognition of a chromosome truncation site associated with alpha thalassaemia by human telomerase. Nature 353, 454–456 (1991). · DOI
  14. Joyce, C.M. & Steitz, T.A. Function and structure relationships in DNA polymerases. Annu. Rev. Biochem. 63, 777–822 (1994). · DOI
  15. Sousa, R. Structural and mechanistic relationships between nucleic acid polymerases. TIBS 21, 186–190 (1996).
  16. Autexier, C., Pruzan, R., Funk, W.D. & Greider, C.W. Reconstitution of human telomerase activity and identification of a minimal functional region of the human telomerase RNA. EMBO J. 15, 5928–5935 (1996). · DOI
  17. Perez, D.H., Vilander, L., Andrews, W.H. & Holmes, R. Human formyl peptide receptor ligand binding domain(s): studies using an improved mutagenesis/expression vector reveal a novel mechanism or the regulation of receptor occupancy. J. Biol. Chem. 269, 22485–22487 (1994). · DOI
  18. Lin, J.-H., Wang, M., Andrews, W.H., Wydro, R. & Morser, J. Expression efficiency of the human thrombomodulin-encoding gene in various vector and host systems. Gene 147, 287–292 (1994). · DOI
  19. Melton, D.A., Krieg, P.A., Rebagliati, M.R., Maniatis, T., Zinn, K. & Green, M.R. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res. 12, 7035–7056 (1984). · DOI · Paper page
  20. Studier, F.W. & Moffatt, B.A. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189, 113–130 (1986). · DOI
  21. Kim, N.W. & Wu, F. Advances in quantification and characterization of telomerase activity by the telomeric repeat amplification protocol (TRAP). Nucleic Acids Res. 25, 2595–2597 (1997). · DOI
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Cited by

Metadata

Authors
Weinrich, S. L., Pruzan, R., Ma, L., Ouellette, M., Tesmer, V. M., Holt, S. E., Bodnar, A. G., Lichtsteiner, S., Kim, N. W., Trager, J. B., Taylor, R. D., Carlos, R., Andrews, W. H., Wright, W. E., Shay, J. W., Harley, C. B., & Morin, G. B.
Venue
Nature Genetics
Publisher
Springer Science and Business Media LLC
Published
1997-12
Volume
17
Issue
4
Pages
498-502
ISSN
  • 1061-4036
  • 1546-1718
DOI
10.1038/ng1297-498
Cited by
766
References
8
Language
en
Licence
www.springer.com/tdm (tdm)

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