Cited by 123Hematopoietic Stem Cell TransplantationMicroRNA in disease regulationDevelopmental Biology and Gene Regulation

Macrophage development from HSCs requires PU.1-coordinated microRNA expression

Open access
Ghani, Saeed, Riemke, Pia, Schönheit, Jörg, Lenze, Dido, Stumm, Jürgen, Hoogenkamp, Maarten, Lagendijk, Anne, Heinz, Sven +5 more·2011·Blood

Abstract

The differentiation of HSCs into myeloid lineages requires the transcription factor PU.1. Whereas PU.1-dependent induction of myeloid-specific target genes has been intensively studied, negative regulation of stem cell or alternate lineage programs remains incompletely characterized. To test for such negative regulatory events, we searched for PU.1-controlled microRNAs (miRs) by expression profiling using a PU.1-inducible myeloid progenitor cell line model. We provide evidence that PU.1 directly controls expression of at least 4 of these miRs (miR-146a, miR-342, miR-338, and miR-155) through temporally dynamic occupation of binding sites within regulatory chromatin regions adjacent to their genomic coding loci. Ectopic expression of the most robustly induced PU.1 target miR, miR-146a, directed the selective differentiation of HSCs into functional peritoneal macrophages in mouse transplantation assays. In agreement with this observation, disruption of Dicer expression or specific antagonization of miR-146a function inhibited the formation of macrophages during early zebrafish (Danio rerio) development. In the present study, we describe a PU.1-orchestrated miR program that mediates key functions of PU.1 during myeloid differentiation.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1182/blood-2011-02-335141

Cites

52 of 52 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

1 of the 53 references couldn’t be matched to a known paper.

Cited by

Sorted by citations

Authors
Ghani, S., Riemke, P., Schönheit, J., Lenze, D., Stumm, J., Hoogenkamp, M., Lagendijk, A., Heinz, S., Bonifer, C., Bakkers, J., Abdelilah-Seyfried, S., Hummel, M., & Rosenbauer, F.
Affiliations
  • Max Delbrück Center (DE)
  • Charité – Universitätsmedizin Berlin (DE)
  • University of Leeds (GB)
  • Royal Netherlands Academy of Arts and Sciences (NL)
  • University of California San Diego (US)
  • Max-Delbrück-Center for Molecular Medicine, Berlin, Germany;
Venue
Blood
Published
2011-08-25
Volume
118
Issue
8
Pages
2275-2284
ISSN
  • 0006-4971 (ISSN-L)
  • 1528-0020
OpenAlex
W1997338207
Cited by
123
References
53
Citations by year
2026: 6 · 2025: 3 · 2024: 4 · 2023: 5 · 2022: 7 · 2021: 3 · 2020: 6 · 2019: 9 · 2018: 8 · 2017: 6 · 2016: 10 · 2015: 5 · 2014: 11 · 2013: 28 · 2012: 11
FWCI
10.75
Type
journal article
Language
en
Open access
Locations
  • Blood (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • KNAW Research Portal (The Royal Netherlands Academy of Arts and Sciences) (publishedVersion, other-oa)
  • Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
  • MDC Repository (Max-Delbrueck-Center for Molecular Medicine) (submittedVersion)
  • University of Birmingham Research Portal (University of Birmingham) (submittedVersion)
  • KNAW research portal (Royal Academy of Art and Sciences (KNAW)) (submittedVersion, other-oa)
  • University of Groningen research database (University of Groningen / Centre for Information Technology) (submittedVersion)
Topics
  • Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
Keywords
PU.1, microRNA, hematopoietic stem cells, macrophage differentiation, myeloid differentiation, miR-146a, miR-155, miR-342, miR-338, Dicer, transcriptional regulation, peritoneal macrophages, zebrafish development, chromatin binding sites
MeSH terms
Proto-Oncogene Protein Spi-1, Animals, Cell Differentiation, Cell Line, Hematopoietic Stem Cells, Mice, Inbred C57BL, Proto-Oncogene Proteins, Zebrafish, Trans-Activators, Macrophages, Peritoneal, Cell Lineage, RNA, Small Interfering, MicroRNAs, Myelopoiesis, Mice, In Vitro Techniques

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review