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Localization of Rac2 via the C terminus and aspartic acid 150 specifies superoxide generation, actin polarity and chemotaxis in neutrophils

Filippi, Marie-Dominique, Harris, Chad E, Meller, Jarek, Gu, Yi, Zheng, Yi, Williams, David A·2004·Nature Immunology
DOI 10.1038/ni1081
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Abstract

Despite having a high degree of sequence similarity, the Rho guanosine triphosphatases Rac1 and Rac2 regulate distinct functions in neutrophils. Here we demonstrate that the unique Rac2 localization and functions in neutrophils are regulated by two separate C-terminal motifs, the hypervariable domain and aspartic acid 150, one of which has not previously been linked to the function of Rho GTPases. In addition, we show an unexpected dependence of Rac1 localization on Rac2 activity in these same cells, demonstrating a degree of crosstalk between two closely related Rho GTPases. Thus, we have defined specific sequences in Rac that specify subcellular localization and determine the specificity of Rac2 in neutrophil chemotaxis and superoxide generation.

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Cited by

Metadata

Authors
Filippi, M.-D., Harris, C. E., Meller, J., Gu, Y., Zheng, Y., & Williams, D. A.
Venue
Nature Immunology
Publisher
Springer Science and Business Media LLC
Published
2004-05-30
Volume
5
Issue
7
Pages
744-751
ISSN
  • 1529-2908
  • 1529-2916
DOI
10.1038/ni1081
Cited by
110
References
0
Language
en
Licence
www.springer.com/tdm (tdm)

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