The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Generation of stable monoclonal antibody–producing B cell receptor–positive human memory B cells by genetic programming
Open accessAbstract
The B cell lymphoma-6 (Bcl-6) and Bcl-xL proteins are expressed in germinal center B cells and enable them to endure the proliferative and mutagenic environment of the germinal center. By introducing these genes into peripheral blood memory B cells and culturing these cells with two factors produced by follicular helper T cells, CD40 ligand (CD40L) and interleukin-21 (IL-21), we convert them to highly proliferating, cell surface B cell receptor (BCR)–positive, immunoglobulin-secreting B cells with features of germinal center B cells, including expression of activation-induced cytidine deaminase (AID). We generated cloned lines of B cells specific for respiratory syncytial virus and used these cells as a source of antibodies that effectively neutralized this virus in vivo. This method provides a new tool to study B cell biology and signal transduction through antigen-specific B cell receptors and for the rapid generation of high-affinity human monoclonal antibodies.
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Metadata
- Authors
- Kwakkenbos, M. J., Diehl, S. A., Yasuda, E., Bakker, A. Q., van Geelen, C. M. M., Lukens, M. V., van Bleek, G. M., Widjojoatmodjo, M. N., Bogers, W. M. J. M., Mei, H., Radbruch, A., Scheeren, F. A., Spits, H., & Beaumont, T.
- Venue
- Nature Medicine
- Published
- 2009-12-20
- Volume
- 16
- Issue
- 1
- Pages
- 123-128
- ISSN
- 1078-8956 (ISSN-L)
- 1546-170X
- OpenAlex
- W2143237309
- Cited by
- 300
- References
- 37
- FWCI
- 4.20
- Type
- journal article
- Language
- en
- Open access
- green
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