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Cited by 300

The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.

Generation of stable monoclonal antibody–producing B cell receptor–positive human memory B cells by genetic programming

Open access
Kwakkenbos, Mark J, Diehl, Sean A, Yasuda, Etsuko, Bakker, Arjen Q, van Geelen, Caroline M M, Lukens, Michaël V, van Bleek, Grada M, Widjojoatmodjo, Myra N, Bogers, Willy M J M, Mei, Henrik, Radbruch, Andreas, Scheeren, Ferenc A, Spits, Hergen, Beaumont, Tim·2010·Nature Medicine
DOI 10.1038/nm.2071
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Abstract

The B cell lymphoma-6 (Bcl-6) and Bcl-xL proteins are expressed in germinal center B cells and enable them to endure the proliferative and mutagenic environment of the germinal center. By introducing these genes into peripheral blood memory B cells and culturing these cells with two factors produced by follicular helper T cells, CD40 ligand (CD40L) and interleukin-21 (IL-21), we convert them to highly proliferating, cell surface B cell receptor (BCR)–positive, immunoglobulin-secreting B cells with features of germinal center B cells, including expression of activation-induced cytidine deaminase (AID). We generated cloned lines of B cells specific for respiratory syncytial virus and used these cells as a source of antibodies that effectively neutralized this virus in vivo. This method provides a new tool to study B cell biology and signal transduction through antigen-specific B cell receptors and for the rapid generation of high-affinity human monoclonal antibodies.

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Crossref · 10.1038/nm.2071

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Metadata

Authors
Kwakkenbos, M. J., Diehl, S. A., Yasuda, E., Bakker, A. Q., van Geelen, C. M. M., Lukens, M. V., van Bleek, G. M., Widjojoatmodjo, M. N., Bogers, W. M. J. M., Mei, H., Radbruch, A., Scheeren, F. A., Spits, H., & Beaumont, T.
Venue
Nature Medicine
Publisher
Springer Science and Business Media LLC
Published
2009-12-20
Volume
16
Issue
1
Pages
123-128
ISSN
  • 1078-8956 (ISSN-L)
  • 1546-170X
DOI
10.1038/nm.2071
OpenAlex
W2143237309
Cited by
300
References
37
FWCI
4.20
Type
journal article
Language
en
Open access
green

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