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Molecular modeling, organ culture and reverse genetics for a newly identified human rhinovirus C
Open accessAbstract
A recently recognized human rhinovirus species C (HRV-C) is associated with up to half of HRV infections in young children. Here we propagated two HRV-C isolates ex vivo in organ culture of nasal epithelial cells, sequenced a new C15 isolate and developed the first, to our knowledge, reverse genetics system for HRV-C. Using contact points for the known HRV receptors, intercellular adhesion molecule-1 (ICAM-1) and low-density lipoprotein receptor (LDLR), inter- and intraspecies footprint analyses predicted a unique cell attachment site for HRV-Cs. Antibodies directed to binding sites for HRV-A and -B failed to inhibit HRV-C attachment, consistent with the alternative receptor footprint. HRV-A and HRV-B infected HeLa and WisL cells but HRV-C did not. However, HRV-C RNA synthesized in vitro and transfected into both cell types resulted in cytopathic effect and recovery of functional virus, indicating that the viral attachment mechanism is a primary distinguishing feature of HRV-C.
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Metadata
- Authors
- Bochkov, Y. A., Palmenberg, A. C., Lee, W.-M., Rathe, J. A., Amineva, S. P., Sun, X., Pasic, T. R., Jarjour, N. N., Liggett, S. B., & Gern, J. E.
- Venue
- Nature Medicine
- Published
- 2011-04-10
- Volume
- 17
- Issue
- 5
- Pages
- 627-632
- ISSN
- 1078-8956 (ISSN-L)
- 1546-170X
- OpenAlex
- W2158725700
- Cited by
- 203
- References
- 0
- FWCI
- 16.20
- Type
- journal article
- Language
- en
- Open access
- bronze
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