2-hydroxyglutarate detection by magnetic resonance spectroscopy in IDH-mutated patients with gliomas
Open accessAbstract
Mutations in isocitrate dehydrogenases 1 and 2 (IDH1 and IDH2) have been shown to be present in most World Health Organization grade 2 and grade 3 gliomas in adults. These mutations are associated with the accumulation of 2-hydroxyglutarate (2HG) in the tumor. Here we report the noninvasive detection of 2HG by proton magnetic resonance spectroscopy (MRS). We developed and optimized the pulse sequence with numerical and phantom analyses for 2HG detection, and we estimated the concentrations of 2HG using spectral fitting in the tumors of 30 subjects. Detection of 2HG correlated with mutations in IDH1 or IDH2 and with increased levels of D-2HG by mass spectrometry of the resected tumors. Noninvasive detection of 2HG may prove to be a valuable diagnostic and prognostic biomarker.
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2-HG abundance has been used for 1H magnetic resonance spectroscopy-based imaging of IDH-mutant gliomas.
“The abundance of 2-HG has been utilized for 1H magnetic resonance spectroscopy-based imaging of IDH-mutant gliomas (Choi et al., 2012).”
From The emerging hallmarks of cancer metabolism (2016) · cites this paper as Choi et al., 2012
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- Authors
- Choi, C., Ganji, S. K., DeBerardinis, R. J., Hatanpaa, K. J., Rakheja, D., Kovacs, Z., Yang, X.-L., Mashimo, T., Raisanen, J. M., Marin-Valencia, I., Pascual, J. M., Madden, C. J., Mickey, B. E., Malloy, C. R., Bachoo, R. M., & Maher, E. A.
- Affiliations
- The University of Texas Southwestern Medical Center (US)
- Harold C. Simmons Comprehensive Cancer Center (US)
- Children's Medical Center (US)
- VA North Texas Health Care System (US)
- Corresponding author
- Choi, Changho
- Maher, Elizabeth A
- ORCID
- Venue
- Nature Medicine
- Published
- 2012-01-26
- Volume
- 18
- Issue
- 4
- Pages
- 624-629
- ISSN
- 1078-8956 (ISSN-L)
- 1546-170X
- PMC
- PMC3615719
- OpenAlex
- W2058562945
- Cited by
- 839
- References
- 31
- Citations by year
- 2026: 36 · 2025: 40 · 2024: 40 · 2023: 64 · 2022: 49 · 2021: 63 · 2020: 72 · 2019: 73 · 2018: 67 · 2017: 85 · 2016: 75 · 2015: 53 · 2014: 52 · 2013: 44 · 2012: 24
- FWCI
- 26.34
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Medicine (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- Cancer Research Institute
- Cancer Prevention and Research Institute of Texas
- University of Texas Southwestern Medical Center
- National Institutes of Health
- National Cancer Institute (R01CA154843, R01CA157996, R21CA159128)
- National Institute of Neurological Disorders and Stroke (RC1NS0760675, RC1NS070675)
- National Center for Research Resources (P41RR002584, RR‐02584)
- Topics
- Health Sciences › Medicine › Genetics › Glioma Diagnosis and Treatment
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Advanced MRI Techniques and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Keywords
- 2-hydroxyglutarate, IDH1 mutation, IDH2 mutation, glioma, 1H NMR, non-invasive cancer detection, metabolic biomarkers, pulse sequence optimization
- MeSH terms
- Algorithms, Aspartic Acid, Brain, Brain Mapping, Brain Neoplasms, Choline, Creatine, Female, Glioma, Glutarates, Humans, Isocitrate Dehydrogenase, Male, Mutation, Magnetic Resonance Spectroscopy, Statistics as Topic
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