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In situ cell manipulation through enzymatic hydrogel photopatterning
Open accessAbstract
The physicochemical properties of hydrogels can be manipulated in both space and time through the controlled application of a light beam. However, methods for hydrogel photopatterning either fail to maintain the bioactivity of fragile proteins and are thus limited to short peptides, or have been used in hydrogels that often do not support three-dimensional (3D) cell growth. Here, we show that the 3D invasion of primary human mesenchymal stem cells can be spatiotemporally controlled by micropatterning the hydrogel with desired extracellular matrix (ECM) proteins and growth factors. A peptide substrate of activated transglutaminase factor XIII (FXIIIa)--a key ECM crosslinking enzyme--is rendered photosensitive by masking its active site with a photolabile cage group. Covalent incorporation of the caged FXIIIa substrate into poly(ethylene glycol) hydrogels and subsequent laser-scanning lithography affords highly localized biomolecule tethering. This approach for the 3D manipulation of cells within gels should open up avenues for the study and manipulation of cell signalling.
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“PEG-based matrices that are responsive to physical (light) or chemical (enzymes) stimuli have been created to reproduce the dynamics of the reparative process, by modulating the local milieu in time and space (146).”
From Wound repair and regeneration: Mechanisms, signaling, and translation (2014) · cites this paper as [146]
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“Synthetic hydrogels can be functionalized with biologically active moieties that permit the growth and spread of cells146–151; concentration and spacing of these cues can be changed independently152–155.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [154]
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Metadata
- Authors
- Mosiewicz, K. A., Kolb, L., van der Vlies, A. J., Martino, M. M., Lienemann, P. S., Hubbell, J. A., Ehrbar, M., & Lutolf, M. P.
- Affiliations
- École Polytechnique Fédérale de Lausanne (CH)
- Immunology Frontier Research Center, The University of Osaka (JP)
- University of Zurich (CH)
- Venue
- Nature Materials
- Published
- 2013-10-13
- Volume
- 12
- Issue
- 11
- Pages
- 1072-1078
- ISSN
- 1476-1122 (ISSN-L)
- 1476-4660
- OpenAlex
- W2099164785
- Cited by
- 308
- References
- 36
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 11 · 2023: 18 · 2022: 20 · 2021: 22 · 2020: 28 · 2019: 18 · 2018: 34 · 2017: 38 · 2016: 43 · 2015: 37 · 2014: 27 · 2013: 4
- FWCI
- 17.04
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Nature Materials (publishedVersion)
- PubMed (publishedVersion)
- Zurich Open Repository and Archive (University of Zurich) (acceptedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- Zurich Open Repository and Archive (University of Zurich) (submittedVersion)
- Zurich Open Repository and Archive (University of Zurich)
- Funders
- École Polytechnique Fédérale de Lausanne
- Topics
- Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- enzymatic crosslinking, photolabile protecting groups, poly(ethylene glycol) hydrogels, mesenchymal stem cells, extracellular matrix proteins, growth factor immobilization, signal transduction
- MeSH terms
- Amino Acid Sequence, Animals, Enzymes, Immobilized, Humans, Light, Photolysis, Polyethylene Glycols, Rabbits, Hydrogels, Factor XIIIa, Mice, Microtechnology, Mesenchymal Stem Cells, Cell Engineering
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