The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
In situ cell manipulation through enzymatic hydrogel photopatterning
Open accessAbstract
The physicochemical properties of hydrogels can be manipulated in both space and time through the controlled application of a light beam. However, methods for hydrogel photopatterning either fail to maintain the bioactivity of fragile proteins and are thus limited to short peptides, or have been used in hydrogels that often do not support three-dimensional (3D) cell growth. Here, we show that the 3D invasion of primary human mesenchymal stem cells can be spatiotemporally controlled by micropatterning the hydrogel with desired extracellular matrix (ECM) proteins and growth factors. A peptide substrate of activated transglutaminase factor XIII (FXIIIa)--a key ECM crosslinking enzyme--is rendered photosensitive by masking its active site with a photolabile cage group. Covalent incorporation of the caged FXIIIa substrate into poly(ethylene glycol) hydrogels and subsequent laser-scanning lithography affords highly localized biomolecule tethering. This approach for the 3D manipulation of cells within gels should open up avenues for the study and manipulation of cell signalling.
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“PEG-based matrices that are responsive to physical (light) or chemical (enzymes) stimuli have been created to reproduce the dynamics of the reparative process, by modulating the local milieu in time and space (146).”
From Wound repair and regeneration: Mechanisms, signaling, and translation (2014) · cites this paper as [146]
In the citing paper
“Synthetic hydrogels can be functionalized with biologically active moieties that permit the growth and spread of cells146–151; concentration and spacing of these cues can be changed independently152–155.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [154]
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Citations
Cites
Matrix elasticity directs stem cell lineage specification
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Self-citationDesigning materials for biology and medicine
Langer, R., & Tirrell, D. A. (2004) · Nature · doi:10.1038/nature02388 · Cited by 3,198
Photodegradable Hydrogels for Dynamic Tuning of Physical and Chemical Properties
Kloxin, A. M., Kasko, A. M., Salinas, C. N., & Anseth, K. S. (2009) · Science · doi:10.1126/science.1169494 · Cited by 1,748
Substrate Elasticity Regulates Skeletal Muscle Stem Cell Self-Renewal in Culture
Gilbert, P. M., Havenstrite, K. L., Magnusson, K. E. G., Sacco, A., Leonardi, N. A., Kraft, P., Nguyen, N. K., Thrun, S., Lutolf, M. P., & Blau, H. M. (2010) · Science · doi:10.1126/science.1191035 · Cited by 1,587
Transglutaminases: crosslinking enzymes with pleiotropic functions
Lorand, L., & Graham, R. M. (2003) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1014 · Cited by 1,439
Lee, K., Silva, E. A., & Mooney, D. J. (2010) · Journal of The Royal Society Interface · doi:10.1098/rsif.2010.0223 · Cited by 1,424
Caged compounds: photorelease technology for control of cellular chemistry and physiology
Ellis‐Davies, G. C. R. (2007) · Nature Methods · doi:10.1038/nmeth1072 · Cited by 959
Sequential click reactions for synthesizing and patterning three-dimensional cell microenvironments
DeForest, C. A., Polizzotti, B. D., & Anseth, K. S. (2009) · Nature Materials · doi:10.1038/nmat2473 · Cited by 853
A photolabile hydrogel for guided three-dimensional cell growth and migration
Luo, Y., & Shoichet, M. S. (2004) · Nature Materials · doi:10.1038/nmat1092 · Cited by 801
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Cell-Material Interaction in Sustainable Biomedical Engineering
Ghosh, S., Sen, A., Digar, A., Basu, B., Dutta, S., Mukherjee, S., & Singh, S. K. (2025) · Springer eBooks · doi:10.1007/978-3-031-91790-5_8
Multiscale cytometry of 3D cell cultures in microfluidic hydrogel arrays
Tomasi, R. F.-X. (2016) · theses.fr (ABES) · doi:10.70675/74b0d94cza3c4z464dz90c9z64a0735ee93b
“Alternatively, synthetic hydrogels can be engineered and provide a high level of flexibility [10] regarding composition and properties.”
BackgroundDalier, F. (2016) · doi:10.70675/aac5eeb5zc2d3z4d13z8be4zf923a1e2cb65
“Recent development of this principle propose the use of biocompatible mild photocleavable enzymes (Mosiewicz et al., 2013), or thiol-based chemistry to modify (irreversibly) hydrogel stiffness with spatiotemporal control (Mosiewicz et al., 2014).”
Biomaterials as blueprints: Architecting functional vascularized cardiac organoids
Cao, Y., Wang, C., Gao, S., & Xiao, Z. (2026) · Chemical Engineering Journal · doi:10.1016/j.cej.2026.177392
Applications in Biomedical and Bioengineering
(2026) · Royal Society of Chemistry eBooks · doi:10.1039/9781837678525-00428
Metadata
- Authors
- Mosiewicz, K. A., Kolb, L., van der Vlies, A. J., Martino, M. M., Lienemann, P. S., Hubbell, J. A., Ehrbar, M., & Lutolf, M. P.
- Affiliations
- École Polytechnique Fédérale de Lausanne (CH)
- Immunology Frontier Research Center, The University of Osaka (JP)
- University of Zurich (CH)
- Venue
- Nature Materials
- Published
- 2013-10-13
- Volume
- 12
- Issue
- 11
- Pages
- 1072-1078
- ISSN
- 1476-1122 (ISSN-L)
- 1476-4660
- OpenAlex
- W2099164785
- Cited by
- 308
- References
- 36
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 11 · 2023: 18 · 2022: 20 · 2021: 22 · 2020: 28 · 2019: 18 · 2018: 34 · 2017: 38 · 2016: 43 · 2015: 37 · 2014: 27 · 2013: 4
- FWCI
- 17.04
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Nature Materials (publishedVersion)
- PubMed (publishedVersion)
- Zurich Open Repository and Archive (University of Zurich) (acceptedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- Zurich Open Repository and Archive (University of Zurich) (submittedVersion)
- Zurich Open Repository and Archive (University of Zurich)
- Funders
- École Polytechnique Fédérale de Lausanne
- Topics
- Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- enzymatic crosslinking, photolabile protecting groups, poly(ethylene glycol) hydrogels, mesenchymal stem cells, extracellular matrix proteins, growth factor immobilization, signal transduction
- MeSH terms
- Amino Acid Sequence, Animals, Enzymes, Immobilized, Humans, Light, Photolysis, Polyethylene Glycols, Rabbits, Hydrogels, Factor XIIIa, Mice, Microtechnology, Mesenchymal Stem Cells, Cell Engineering
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