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In situ cell manipulation through enzymatic hydrogel photopatterning
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The physicochemical properties of hydrogels can be manipulated in both space and time through the controlled application of a light beam. However, methods for hydrogel photopatterning either fail to maintain the bioactivity of fragile proteins and are thus limited to short peptides, or have been used in hydrogels that often do not support three-dimensional (3D) cell growth. Here, we show that the 3D invasion of primary human mesenchymal stem cells can be spatiotemporally controlled by micropatterning the hydrogel with desired extracellular matrix (ECM) proteins and growth factors. A peptide substrate of activated transglutaminase factor XIII (FXIIIa)--a key ECM crosslinking enzyme--is rendered photosensitive by masking its active site with a photolabile cage group. Covalent incorporation of the caged FXIIIa substrate into poly(ethylene glycol) hydrogels and subsequent laser-scanning lithography affords highly localized biomolecule tethering. This approach for the 3D manipulation of cells within gels should open up avenues for the study and manipulation of cell signalling.
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“PEG-based matrices that are responsive to physical (light) or chemical (enzymes) stimuli have been created to reproduce the dynamics of the reparative process, by modulating the local milieu in time and space (146).”
From Wound repair and regeneration: Mechanisms, signaling, and translation (2014) · cites this paper as [146]
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“Synthetic hydrogels can be functionalized with biologically active moieties that permit the growth and spread of cells146–151; concentration and spacing of these cues can be changed independently152–155.”
From Towards organoid culture without Matrigel (2021) · cites this paper as [154]
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Cites
Matrix elasticity directs stem cell lineage specification
Engler, A. J., Sen, S., Sweeney, H. L., & Discher, D. E. (2006) · Cell · doi:10.1016/j.cell.2006.06.044 · Cited by 13,915
Lutolf, M. P., & Hubbell, J. A. (2005) · Nature Biotechnology · doi:10.1038/nbt1055 · Cited by 4,551
Self-citationDesigning materials for biology and medicine
Langer, R., & Tirrell, D. A. (2004) · Nature · doi:10.1038/nature02388 · Cited by 3,198
Photodegradable Hydrogels for Dynamic Tuning of Physical and Chemical Properties
Kloxin, A. M., Kasko, A. M., Salinas, C. N., & Anseth, K. S. (2009) · Science · doi:10.1126/science.1169494 · Cited by 1,748
Substrate Elasticity Regulates Skeletal Muscle Stem Cell Self-Renewal in Culture
Gilbert, P. M., Havenstrite, K. L., Magnusson, K. E. G., Sacco, A., Leonardi, N. A., Kraft, P., Nguyen, N. K., Thrun, S., Lutolf, M. P., & Blau, H. M. (2010) · Science · doi:10.1126/science.1191035 · Cited by 1,587
Transglutaminases: crosslinking enzymes with pleiotropic functions
Lorand, L., & Graham, R. M. (2003) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1014 · Cited by 1,439
Lee, K., Silva, E. A., & Mooney, D. J. (2010) · Journal of The Royal Society Interface · doi:10.1098/rsif.2010.0223 · Cited by 1,424
Caged compounds: photorelease technology for control of cellular chemistry and physiology
Ellis‐Davies, G. C. R. (2007) · Nature Methods · doi:10.1038/nmeth1072 · Cited by 959
Sequential click reactions for synthesizing and patterning three-dimensional cell microenvironments
DeForest, C. A., Polizzotti, B. D., & Anseth, K. S. (2009) · Nature Materials · doi:10.1038/nmat2473 · Cited by 853
A photolabile hydrogel for guided three-dimensional cell growth and migration
Luo, Y., & Shoichet, M. S. (2004) · Nature Materials · doi:10.1038/nmat1092 · Cited by 801
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Items 101–200 of 307
Qiu, W., Gehlen, J., Bernero, M., Gehre, C., Schädli, G. N., Müller, R., & Qin, X. (2023) · Advanced Functional Materials · doi:10.1002/adfm.202214393 · Cited by 68
Alge, D. L., & Anseth, K. S. (2013) · Nature Materials · doi:10.1038/nmat3794 · Cited by 70
Organoid Technology: Current Standing and Future Perspectives
Shariati, L., Esmaeili, Y., Javanmard, S. H., Bidram, E., & Amini, A. (2021) · Stem Cells · doi:10.1002/stem.3379 · Cited by 71
“The next generation of regenerative therapy as an organ replacement therapy targets the whole organs through multiple cell types with a complex 3D structure.(243) Investigators have placed”
BackgroundUV‐Induced Tetrazole‐Thiol Reaction for Polymer Conjugation and Surface Functionalization
Feng, W., Li, L. X., Yang, C., Welle, A., Trapp, O., & Levkin, P. A. (2015) · Angewandte Chemie International Edition · doi:10.1002/anie.201502954 · Cited by 68
Directed Assembly and Development of Material‐Free Tissues with Complex Architectures
Vrij, E., Rouwkema, J., LaPointe, V. L. S., van Blitterswijk, C., Truckenmüller, R. K., & Rivron, N. C. (2016) · Advanced Materials · doi:10.1002/adma.201505723 · Cited by 65
Substrate-Bound Protein Gradients to Study Haptotaxis
Ricoult, S. G., Kennedy, T. E., & Juncker, D. (2015) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2015.00040 · Cited by 60
Photo-responsive polymers based on ο-Nitrobenzyl derivatives: from structural design to applications
Liu, T., Bao, B., Li, Y., Lin, Q., & Zhu, L. (2023) · Progress in Polymer Science · doi:10.1016/j.progpolymsci.2023.101741 · Cited by 64
Su, T., Tang, Z., He, H., Li, W., Wang, X., Liao, C., Sun, Y., & Wang, Q. (2014) · Chemical Science · doi:10.1039/c4sc01603c · Cited by 62
Engineered Extracellular Matrices as Biomaterials of Tunable Composition and Function
Bourgine, P. E., Gaudiello, E., Pippenger, B. E., Jaquiéry, C., Klein, T., Pigeot, S., Todorov, A., Feliciano, S., Banfi, A., & Martín, I. (2017) · Advanced Functional Materials · doi:10.1002/adfm.201605486 · Cited by 67
Patterned superhydrophobic surfaces to process and characterize biomaterials and 3D cell culture
Neto, A. I., Levkin, P. A., & Mano, J. F. (2018) · Materials Horizons · doi:10.1039/c7mh00877e · Cited by 59
Gharios, R., Francis, R. M., & DeForest, C. A. (2023) · Matter · doi:10.1016/j.matt.2023.10.012 · Cited by 60
Synthesis and High-Throughput Processing of Polymeric Hydrogels for 3D Cell Culture
Lowe, S. B., Tan, V. T. G., Soeriyadi, A. H., Davis, T. P., & Gooding, J. J. (2014) · Bioconjugate Chemistry · doi:10.1021/bc500310v · Cited by 60
Oksdath, M., Perrin, S. L., Bardy, C., Hilder, E. F., DeForest, C. A., Arrua, R. D., & Gomez, G. A. (2018) · APL Bioengineering · doi:10.1063/1.5045124 · Cited by 62
“…limitation, the Lutolf group developed a similar strategy, in which the bioconjugation step is achieved by an enzymatic ligation reaction, intrinsically compatible with biological systems and which lacks the need of complex chemical conjugation on large biomacromolecules (Mosiewicz et al., 2013).”
BackgroundMethodInfluentialHe, X. Y., Sun, A., Li, T., Qian, Y. J., Qian, H., Ling, Y. F., Zhang, L. H., Liu, Q. Y., Peng, T., & Qian, Z. (2020) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2020.116692 · Cited by 64
Bioorthogonal strategies for site-directed decoration of biomaterials with therapeutic proteins
Braun, A. C., Gutmann, M., Lühmann, T. C., & Meinel, L. (2018) · Journal of Controlled Release · doi:10.1016/j.jconrel.2018.01.018 · Cited by 57
Tan, Y., Huang, H. H., Ayers, D. C., & Song, J. (2018) · ACS Central Science · doi:10.1021/acscentsci.8b00170 · Cited by 58
Babu, S., Albertino, F., Omidinia‐Anarkoli, A., & Laporte, L. D. (2021) · Advanced Healthcare Materials · doi:10.1002/adhm.202002221 · Cited by 57
Loebel, C., Broguière, N., Alini, M., Zenobi‐Wong, M., & Eglin, D. (2015) · Biomacromolecules · doi:10.1021/acs.biomac.5b00363 · Cited by 56
Gomez, E. J., Gerhardt, K. P., Judd, J., Tabor, J. J., & Suh, J. (2015) · ACS Nano · doi:10.1021/acsnano.5b05558 · Cited by 56
Biomaterials for 4D stem cell culture
Hilderbrand, A. M., Ovadia, E. M., Rehmann, M. S., Kharkar, P. M., Guo, C., & Kloxin, A. M. (2016) · Current Opinion in Solid State and Materials Science · doi:10.1016/j.cossms.2016.03.002 · Cited by 54
“For example, Lutolf and coworkers formed hydrogels from multi-arm PEG vinyl-sulfone, the MMP-degradable peptide GCRDGPQG↓IWGQDRCG, and a substrate for the enzyme factor XIIIa that was protected by a light-sensitive cage (FK(Nvoc)GGERCG) [67].”
BackgroundDynamic adaptation of mesenchymal stem cell physiology upon exposure to surface micropatterns
Beijer, N. R. M., Nauryzgaliyeva, Z., Arteaga, E. M., Pieuchot, L., Anselme, K., van de Peppel, J., Vasilevich, A. S., Gröen, N., Roumans, N. J. T., Hebels, D. G. A. J., et al. (2019) · Scientific Reports · doi:10.1038/s41598-019-45284-y · Cited by 52
Advancing Organoid Engineering for Tissue Regeneration and Biofunctional Reconstruction
Jin, H., Xue, Z., Liu, J., Ma, B., Yang, J., & Lei, L. (2024) · Biomaterials Research · doi:10.34133/bmr.0016 · Cited by 58
Staruch, R. M. T., Glass, G. E., Rickard, R. F., Hettiaratchy, S. P., & Butler, P. E. M. (2016) · Tissue Engineering Part B Reviews · doi:10.1089/ten.teb.2016.0305 · Cited by 53
“103 Transglutaminase factor XIIIa is a potent natural ECM cross-linking enzyme and, by blocking its active site with a photolabile cage group, it may become photosensitive.”
Photogenerated Aldehydes for Protein Patterns on Hydrogels and Guidance of Cell Behavior
Ming, Z., Fan, J., Bao, C., Xue, Y., Lin, Q., & Zhu, L. (2018) · Advanced Functional Materials · doi:10.1002/adfm.201706918 · Cited by 53
Atallah, P., Schirmer, L., Tsurkan, M. V., Limasale, Y. D. P., Zimmermann, R., Werner, C., & Freudenberg, U. (2018) · Biomaterials · doi:10.1016/j.biomaterials.2018.07.056 · Cited by 55
Cell‐Instructive Multiphasic Gel‐in‐Gel Materials
Kühn, S., Sievers, J., Stoppa, A., Träber, N., Zimmermann, R., Welzel, P. B., & Werner, C. (2020) · Advanced Functional Materials · doi:10.1002/adfm.201908857 · Cited by 53
Concise Review: Organ Engineering: Design, Technology, and Integration
Kaushik, G. N., Leijten, J., & Khademhosseini, A. (2016) · Stem Cells · doi:10.1002/stem.2502 · Cited by 52
Surface Patterning of Hydrogel Biomaterials to Probe and Direct Cell–Matrix Interactions
Munoz‐Robles, B. G., Kopyeva, I., & DeForest, C. A. (2020) · Advanced Materials Interfaces · doi:10.1002/admi.202001198 · Cited by 53
Microscale patterning of hydrogel stiffness through light-triggered uncaging of thiols
Mosiewicz, K. A., Kolb, L., van der Vlies, A. J., & Lütolf, M. P. (2014) · Biomaterials Science · doi:10.1039/c4bm00262h · Cited by 51
Self-citationModular and orthogonal synthesis of hybrid polymers and networks
Liu, S., Dicker, K. T., & Jia, X. (2015) · Chemical Communications · doi:10.1039/c4cc09568e · Cited by 54
Impact of the Molecular Environment on Thiol–Ene Coupling For Biofunctionalization and Conjugation
Colak, B., Silva, J. C. S. D., Soares, T. A., & Gautrot, J. E. (2016) · Bioconjugate Chemistry · doi:10.1021/acs.bioconjchem.6b00349 · Cited by 50
Bioprinted microvasculature: progressing from structure to function
Seymour, A. J., Westerfield, A. D., Cornelius, V., Skylar‐Scott, M. A., & Heilshorn, S. C. (2022) · Biofabrication · doi:10.1088/1758-5090/ac4fb5 · Cited by 56
“There is also potential for photocaged chemistry to be used for enzymatic and site-specific tethering or release of growth factors within hydrogels [95].”
Guided Homing of Cells in Multi‐Photon Microfabricated Bioscaffolds
Skylar‐Scott, M. A., Liu, M., Wu, Y., Dixit, A. C., & Yanik, M. F. (2016) · Advanced Healthcare Materials · doi:10.1002/adhm.201600082 · Cited by 49
Grow with the Flow: When Morphogenesis Meets Microfluidics
Samal, P., van Blitterswijk, C., Truckenmüller, R. K., & Giselbrecht, S. (2019) · Advanced Materials · doi:10.1002/adma.201805764 · Cited by 48
Application and Prospects of Hydrogel Additive Manufacturing
Zhao, C., Lv, Q., & Wu, W. (2022) · Gels · doi:10.3390/gels8050297 · Cited by 52
Guo, C., Kim, H., Ovadia, E. M., Mourafetis, C. M., Yang, M., Chen, W., & Kloxin, A. M. (2017) · Acta Biomaterialia · doi:10.1016/j.actbio.2017.04.002 · Cited by 46
Gunnewiek, M. K., Luca, A. D., Bollemaat, H. Z., van Blitterswijk, C., Vancso, G. J., Moroni, L., & Benetti, E. M. (2015) · Advanced Healthcare Materials · doi:10.1002/adhm.201400797 · Cited by 45
Membrane distillation by novel hydrogel composite membranes
Salehi, S. M., Profio, G. D., Fontananova, E., Nicoletta, F. P., Curcio, E., & Filpo, G. D. (2016) · Journal of Membrane Science · doi:10.1016/j.memsci.2015.12.062 · Cited by 45
Ming, Z., Ruan, X., Bao, C., Lin, Q., Yang, Y. A., & Zhu, L. (2017) · Advanced Functional Materials · doi:10.1002/adfm.201606258 · Cited by 45
Lau, H. K., Li, L., Jurusik, A., Sabanayagam, C. R., & Kiick, K. L. (2016) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.6b00076 · Cited by 44
Bioresponsive Hydrogels: Chemical Strategies and Perspectives in Tissue Engineering
Sgambato, A., Cipolla, L., & Russo, L. (2016) · Gels · doi:10.3390/gels2040028 · Cited by 42
Benetti, E. M., Gunnewiek, M. K., van Blitterswijk, C., Vancso, G. J., & Moroni, L. (2016) · Journal of Materials Chemistry B · doi:10.1039/c6tb00947f · Cited by 43
Emerging Methods for Enhancing Pluripotent Stem Cell Expansion
Chan, S. W., Rizwan, M., & Yim, E. K. F. (2020) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2020.00070 · Cited by 43
“Consistent with these findings, hESCs and hiPSCs, cultured on PA hydrogels functionalized with a glucosaminoglycan binding peptide, attached better and formed more spread out and robust colonies on substrates coated with and without a Matrigel coating (Musah et al., 2012).”
BackgroundResultOvadia, E. M., Colby, D. W., & Kloxin, A. M. (2018) · Biomaterials Science · doi:10.1039/c8bm00099a · Cited by 40
Chado, G. R., Stoykovich, M. P., & Kaar, J. L. (2016) · ACS Catalysis · doi:10.1021/acscatal.6b01302 · Cited by 42
Gouveia, R. M., Castelletto, V., Hamley, I. W., & Connon, C. J. (2015) · Tissue Engineering Part A · doi:10.1089/ten.tea.2014.0671 · Cited by 41
Zhang, M., Chen, S., Sheng, N., Wang, B., Wu, Z., Liang, Q., & Wang, H. (2021) · Nanoscale · doi:10.1039/d1nr00867f · Cited by 41
Spatiotemporal functional assembly of split protein pairs through a light-activated SpyLigation
Ruskowitz, E. R., Munoz‐Robles, B. G., Strange, A. C., Butcher, C. H., Kurniawan, S., Filteau, J. R., & DeForest, C. A. (2023) · Nature Chemistry · doi:10.1038/s41557-023-01152-x · Cited by 40
Imam, S. S., Hussain, A., Altamimi, M. A., & Alshehri, S. (2021) · Polymers · doi:10.3390/polym13213858 · Cited by 43
Dicker, K. T., Song, J., Moore, A. C., Zhang, H., Li, Y., Burris, D. L., Jia, X., & Fox, J. M. (2018) · Chemical Science · doi:10.1039/c8sc00495a · Cited by 38
Topical hemostatic materials for coagulopathy
Hu, B., Bao, G., Xu, X., & Yang, K. (2022) · Journal of Materials Chemistry B · doi:10.1039/d1tb02523f · Cited by 38
A Fluorescence‐Based Array Screen for Transglutaminase Substrates
Malešević, M., Migge, A., Hertel, T. C., & Pietzsch, M. (2015) · ChemBioChem · doi:10.1002/cbic.201402709 · Cited by 37
Methods for producing microstructured hydrogels for targeted applications in biology
Garcia, C. G., & Kiick, K. L. (2018) · Acta Biomaterialia · doi:10.1016/j.actbio.2018.11.028 · Cited by 37
“Photopatterning allows the generation of almost any desired 2-D pattern, but appropriate design and fabrication of the corresponding photomask are required [61].”
BackgroundStimulus-responsive viral vectors for controlled delivery of therapeutics
Brun, M. J., Gomez, E. J., & Suh, J. (2017) · Journal of Controlled Release · doi:10.1016/j.jconrel.2017.08.021 · Cited by 36
An alternative technique for patterning cells on poly(ethylene glycol) diacrylate hydrogels
Peter, M., & Tayalia, P. (2016) · RSC Advances · doi:10.1039/c6ra08852j · Cited by 35
DNA microbeads for spatio-temporally controlled morphogen release within organoids
Afting, C., Walther, T., Drozdowski, O. M., Schlagheck, C., Schwarz, U. S., Wittbrodt, J., & Göpfrich, K. (2024) · Nature Nanotechnology · doi:10.1038/s41565-024-01779-y · Cited by 38
Koçer, G., & Jonkheijm, P. (2018) · Advanced Healthcare Materials · doi:10.1002/adhm.201701192 · Cited by 34
Magneto‐Driven Gradients of Diamagnetic Objects for Engineering Complex Tissues
Zlotnick, H. M., Clark, A. T., Gullbrand, S. E., Carey, J. L., Cheng, X. M., & Mauck, R. L. (2020) · Advanced Materials · doi:10.1002/adma.202005030 · Cited by 34
Li, Y., Wong, I. Y., & Guo, M. (2022) · Small · doi:10.1002/smll.202107305 · Cited by 39
Chemical Approaches to Dynamically Modulate the Properties of Synthetic Matrices
LeValley, P. J., & Kloxin, A. M. (2018) · ACS Macro Letters · doi:10.1021/acsmacrolett.8b00808 · Cited by 33
Liu, G., Zhou, Y., Zhang, X., & Guo, S. (2022) · Journal of Materials Chemistry B · doi:10.1039/d2tb01044e · Cited by 35
Cha, G. D., Kim, M.-J., Park, O. K., Sunwoo, S., Kang, T., Lee, W. H., Nam, S., Hyeon, T., Choi, S. H., & Kim, D. (2023) · Small Methods · doi:10.1002/smtd.202300032 · Cited by 34
Zhang, Z., Du, J., Li, Y., Wu, J., Yu, F., & Chen, Y. (2017) · Journal of Materials Chemistry B · doi:10.1039/c7tb00883j · Cited by 32
Yi, Z., Zhang, Y., Kootala, S., Hilborn, J. G., & Ossipov, D. A. (2014) · ACS Applied Materials & Interfaces · doi:10.1021/am506926w · Cited by 31
Focal Molography: Coherent Microscopic Detection of Biomolecular Interaction
Fattinger, C. (2014) · Physical Review X · doi:10.1103/physrevx.4.031024 · Cited by 31
Dynamic bioengineered hydrogels as scaffolds for advanced stem cell and organoid culture
Bahlmann, L. C., Fokina, A., & Shoichet, M. S. (2017) · MRS Communications · doi:10.1557/mrc.2017.72 · Cited by 32
Marusina, A. I., Merleev, A. A., Luna, J. I., Olney, L. P., Haigh, N. E., Yoon, D. J., Guo, C., Ovadia, E. M., Shimoda, M., Luxardi, G., et al. (2019) · Stem Cells · doi:10.1002/stem.3105 · Cited by 32
Ma, H., Caldwell, A. S., Azagarsamy, M. A., Rodriguez, A. G., & Anseth, K. S. (2020) · Biomaterials · doi:10.1016/j.biomaterials.2020.120205 · Cited by 31
Click-functionalized hydrogel design for mechanobiology investigations
Hui, E., Sumey, J. L., & Caliari, S. R. (2021) · Molecular Systems Design & Engineering · doi:10.1039/d1me00049g · Cited by 33
Biophysical Control of Bile Duct Epithelial Morphogenesis in Natural and Synthetic Scaffolds
Funfak, A., Bouzhir-Sima, L., Gontran, E., Minier, N., Dupuis‐Williams, P., & Gobaa, S. (2019) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2019.00417 · Cited by 31
Davey, S. K., Aung, A., Agrawal, G., Lim, H. L., Kar, M., & Varghese, S. (2015) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2015.0179 · Cited by 30
“8,12,14,18,19 Among the 3D patterning methods, the use of stereolithography-based approaches has garnered the most attention due to its versatility and ease of construction.”
Zerobin, E., Marković, M., Tomášiková, Z., Qin, X., Ret, D., Steinbauer, P., Kitzmüller, J., Steiger, W., Gruber, P., Ovsianikov, A., et al. (2020) · Journal of Polymer Science · doi:10.1002/pol.20200073 · Cited by 29
Engineered cartilage utilizing fetal cartilage-derived progenitor cells for cartilage repair
Park, D. Y., Min, B., Park, S. R., Oh, H. J., Truong, M., Kim, M., Choi, J., Park, I., & Choi, B. H. (2020) · Scientific Reports · doi:10.1038/s41598-020-62580-0 · Cited by 29
Density gradients at hydrogel interfaces for enhanced cell penetration
Simona, B. R., Hirt, L., Demkó, L., Zambelli, T., Vörös, J., Ehrbar, M., & Milleret, V. (2015) · Biomaterials Science · doi:10.1039/c4bm00416g · Cited by 28
Self-citationPhotolabile ROMP gels using ortho-nitrobenzyl functionalized crosslinkers
Hu, X., Shi, J., & Thomas, S. W. (2015) · Polymer Chemistry · doi:10.1039/c5py00562k · Cited by 27
Wu, T., & Branda, N. R. (2016) · Chemical Communications · doi:10.1039/c6cc03864f · Cited by 27
Kamperman, T., Henke, S., Crispim, J. F., Willemen, N. G. A., Dijkstra, P. J., Lee, W., Offerhaus, H. L., Neubauer, M. P., Smink, A. M., de Vos, P., et al. (2021) · Advanced Materials · doi:10.1002/adma.202102660 · Cited by 27
Synthetic developmental biology: Engineering approaches to guide multicellular organization
Zarkesh, I., Ashtiani, M. K., Shiri, Z., Aran, S., Braun, T. W., & Baharvand, H. (2022) · Stem Cell Reports · doi:10.1016/j.stemcr.2022.02.004 · Cited by 27
“Biomolecule gradients have also been attached to hydrogels in a stepwise or continuous manner (Mosiewicz et al., 2013).”
BackgroundSelf-regenerating giant hyaluronan polymer brushes
Wei, W., Faubel, J. L., Selvakumar, H., Kovari, D. T., Tsao, J., Rivas, F., Mohabir, A. T., Krecker, M. C., Rahbar, E., Hall, A. R., et al. (2019) · Nature Communications · doi:10.1038/s41467-019-13440-7 · Cited by 26
Machining water through laser cutting of nanoparticle-encased water pancakes
Niu, J., Liu, W., Li, J. X., Pang, X., Liu, Y., Zhang, C., Yue, K., Zhou, Y., Xu, F., Li, X., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-39574-3 · Cited by 26
“Hydrogel patterning plays a vital role in the development of multifunctional hydrogels 41 – 43 .”
Navaee, F., Khornian, N., Longet, D., Heub, S., Pasche, S., Weder, G., Kleger, A., Renaud, P., & Braschler, T. M. (2023) · Cells · doi:10.3390/cells12040576 · Cited by 25
“Photolithographic hydrogel in situ patterning [3,22] and hydrogel molding in microgrooves [1,23,24] are typically employed in order to demonstrate a general propensity of various cell types, including cardiomyocytes, to align with microfabricated geometrical features.”
MethodLight-mediated Formation and Patterning of Hydrogels for Cell Culture Applications
Sawicki, L. A., & Kloxin, A. M. (2016) · Journal of Visualized Experiments · doi:10.3791/54462 · Cited by 24
“Subsequently, these gels then may be patterned with biochemical cues to elicit desired cellular responses such as differentiation or invasion.(30,31)”
BackgroundCornwell, D. J., Okesola, B. O., & Smith, D. K. (2014) · Angewandte Chemie · doi:10.1002/ange.201405098 · Cited by 24
Expanding Toolbox of Imageable Protein-Gold Hybrid Materials
Ding, H., Li, H., Wang, X., Zhou, Y., Li, Z., Hiltunen, J. K., Shen, J., & Chen, Z. (2017) · Chemistry of Materials · doi:10.1021/acs.chemmater.7b03011 · Cited by 24
4D Biochemical Photocustomization of Hydrogel Scaffolds for Biomimetic Tissue Engineering
Francis, R. M., & DeForest, C. A. (2023) · Accounts of Materials Research · doi:10.1021/accountsmr.3c00062 · Cited by 23
Reversible control of biomaterial properties for dynamically tuning cell behavior
Fumasi, F. M., Stephanopoulos, N., & Holloway, J. L. (2020) · Journal of Applied Polymer Science · doi:10.1002/app.49058 · Cited by 23
Break Up to Make Up: Utilization of Photocleavable Groups in Biolabeling of Hydrogel Scaffolds
Truong, V. X. (2020) · ChemPhotoChem · doi:10.1002/cptc.202000072 · Cited by 23
Salerno, A., & Netti, P. A. (2023) · Journal of Functional Biomaterials · doi:10.3390/jfb14020101 · Cited by 24
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Metadata
- Authors
- Mosiewicz, K. A., Kolb, L., van der Vlies, A. J., Martino, M. M., Lienemann, P. S., Hubbell, J. A., Ehrbar, M., & Lutolf, M. P.
- Affiliations
- École Polytechnique Fédérale de Lausanne (CH)
- Immunology Frontier Research Center, The University of Osaka (JP)
- University of Zurich (CH)
- Venue
- Nature Materials
- Published
- 2013-10-13
- Volume
- 12
- Issue
- 11
- Pages
- 1072-1078
- ISSN
- 1476-1122 (ISSN-L)
- 1476-4660
- OpenAlex
- W2099164785
- Cited by
- 308
- References
- 36
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 11 · 2023: 18 · 2022: 20 · 2021: 22 · 2020: 28 · 2019: 18 · 2018: 34 · 2017: 38 · 2016: 43 · 2015: 37 · 2014: 27 · 2013: 4
- FWCI
- 17.04
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Nature Materials (publishedVersion)
- PubMed (publishedVersion)
- Zurich Open Repository and Archive (University of Zurich) (acceptedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion)
- Infoscience (Ecole Polytechnique Fédérale de Lausanne) (submittedVersion, cc-by-nc-nd)
- Zurich Open Repository and Archive (University of Zurich) (submittedVersion)
- Zurich Open Repository and Archive (University of Zurich)
- Funders
- École Polytechnique Fédérale de Lausanne
- Topics
- Physical Sciences › Materials Science › Biomaterials › Hydrogels: synthesis, properties, applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular Mechanics and Interactions
- Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
- Keywords
- enzymatic crosslinking, photolabile protecting groups, poly(ethylene glycol) hydrogels, mesenchymal stem cells, extracellular matrix proteins, growth factor immobilization, signal transduction
- MeSH terms
- Amino Acid Sequence, Animals, Enzymes, Immobilized, Humans, Light, Photolysis, Polyethylene Glycols, Rabbits, Hydrogels, Factor XIIIa, Mice, Microtechnology, Mesenchymal Stem Cells, Cell Engineering
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