The DOI matches its Crossref record; 35 of the 35 references it cites were found in a registry or an index.
Transcription upregulation via force-induced direct stretching of chromatin
Open accessAbstract
Mechanical forces play critical roles in the function of living cells. However, the underlying mechanisms of how forces influence nuclear events remain elusive. Here, we show that chromatin deformation as well as force-induced transcription of a green-fluorescent-protein (GFP) tagged bacterial-chromosome dihydrofolate reductase (DHFR) transgene can be visualized in a living cell by using three-dimensional magnetic twisting cytometry to apply local stresses on the cell surface via an Arg-Gly-Asp-coated magnetic bead. Chromatin stretching depended on loading direction. DHFR transcription upregulation was sensitive to load direction and proportional to the magnitude of chromatin stretching. Disrupting filamentous actin or inhibiting actomyosin contraction abrogated or attenuated force-induced DHFR transcription, whereas activating endogenous contraction upregulated force-induced DHFR transcription. Our findings suggest that local stresses applied to integrins propagate from the tensed actin cytoskeleton to the LINC complex and then through lamina-chromatin interactions to directly stretch chromatin and upregulate transcription.
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Cites
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Cited by
Mechanical forces direct stem cell behaviour in development and regeneration
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Force triggers YAP nuclear entry by regulating transport across nuclear pores
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Integrins as biomechanical sensors of the microenvironment
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Lamina-Associated Domains: Links with Chromosome Architecture, Heterochromatin, and Gene Repression
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Metadata
- Authors
- Tajik, A., Zhang, Y., Wei, F., Sun, J., Jia, Q., Zhou, W., Singh, R., Khanna, N., Belmont, A. S., & Wang, N.
- Venue
- Nature Materials
- Published
- 2016-08-22
- Volume
- 15
- Issue
- 12
- Pages
- 1287-1296
- ISSN
- 1476-1122 (ISSN-L)
- 1476-4660
- OpenAlex
- W2508739522
- Cited by
- 629
- References
- 35
- FWCI
- 32.07
- Type
- journal article
- Language
- en
- Open access
- green
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- Tajik, A., Zhang, Y., Wei, F., Sun, J., Jia, Q., Zhou, W., Singh, R., Khanna, N., Belmont, A. S., & Wang, N. (2016). Transcription upregulation via force-induced direct stretching of chromatin. Nature Materials. https://doi.org/10.1038/nmat4729
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