Cited by 65Microbial Metabolic Engineering and BioproductionCRISPR and Genetic EngineeringBacterial Genetics and Biotechnology

Programming biological operating systems: genome design, assembly and activation.

Gibson, Daniel G·2014·Nature Methods

Abstract

The DNA technologies developed over the past 20 years for reading and writing the genetic code converged when the first synthetic cell was created 4 years ago. An outcome of this work has been an extraordinary set of tools for synthesizing, assembling, engineering and transplanting whole bacterial genomes. Technical progress, options and applications for bacterial genome design, assembly and activation are discussed.

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Crossref · 10.1038/nmeth.2894

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Authors
Gibson, D. G.
Affiliations
J. Craig Venter Institute (US)
Corresponding author
Gibson, Daniel G
Venue
Nature Methods
Published
2014-04-29
Volume
11
Issue
5
Pages
521-526
ISSN
  • 1548-7091 (ISSN-L)
  • 1548-7105
OpenAlex
W1970045399
Cited by
65
References
50
Citations by year
2026: 3 · 2025: 2 · 2024: 5 · 2023: 2 · 2021: 6 · 2020: 6 · 2019: 4 · 2018: 5 · 2017: 10 · 2016: 9 · 2015: 9 · 2014: 4
FWCI
4.37
Type
journal article
Language
en
Open access
closed
Locations
  • Nature Methods (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › CRISPR and Genetic Engineering
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
synthetic biology, synthetic genome, bacterial genome assembly, genome activation, genome design, genome editing, artificial cells, genome synthesis
MeSH terms
Biotechnology, Cell-Free System, Cloning, Molecular, Deoxyribonucleases, DNA, Gene Expression Regulation, Genetic Engineering, Genetic Vectors, Mycoplasma, Saccharomyces cerevisiae, Genome, Bacterial, Systems Biology, Synthetic Biology

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